| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 473,924 | A→C | V461G (GTC→GGC) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 473,924 | 0 | A | C | 99.7% | 1284.8 / NA | 320 | V461G (GTC→GGC) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (193/125); total (194/126) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 331 total aligned reads displayed.
AGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGA > REL606/473852‑473996 | tgCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGcccc < 1:4385761/75‑1 (MQ=255)aGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGcccc < 1:4101140/76‑1 (MQ=255)aGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGcccc > 1:644343/1‑76 (MQ=255) cATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCcgc > 1:7562166/1‑76 (MQ=255) aTTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCcgcg > 1:1006280/1‑76 (MQ=255) aTTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCcgcg > 1:303369/1‑76 (MQ=255) ttGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGctc < 2:8464300/76‑1 (MQ=255) ttGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGctc < 2:8736397/76‑1 (MQ=255) tGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGctct < 2:6080967/76‑1 (MQ=255) tGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGctct > 1:9505157/1‑76 (MQ=255) tGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGctct < 2:3846147/76‑1 (MQ=255) gCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGAGCGGCCCCCGCGCtctc > 1:2283382/1‑76 (MQ=255) cacaTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCg > 1:2483627/1‑76 (MQ=255) cacaTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCg < 2:8370053/76‑1 (MQ=255) cacaTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCg < 2:1296235/76‑1 (MQ=255) acaTTCCAGATTCCCCAGTTGCATAAGTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGc < 2:2273491/76‑1 (MQ=255) caTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCa > 2:5735829/1‑76 (MQ=255) caTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCa < 1:4926904/76‑1 (MQ=255) aTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAg < 2:4841705/76‑1 (MQ=255) aTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAg < 2:446438/76‑1 (MQ=255) aTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAg < 2:5762173/76‑1 (MQ=255) ccAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtc < 1:5948067/76‑1 (MQ=255) ccAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtc < 2:1693894/76‑1 (MQ=255) ccAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtc > 1:1676740/1‑76 (MQ=255) cAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtca < 1:8505014/76‑1 (MQ=255) gATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcatc > 1:8019601/1‑76 (MQ=255) gATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcatc < 1:502723/76‑1 (MQ=255) gATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcatc < 1:5402/76‑1 (MQ=255) gATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcatc > 2:5091273/1‑76 (MQ=255) gATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcatc > 2:4572309/1‑76 (MQ=255) aggcgcCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcatca < 2:2947681/74‑1 (MQ=255) aTGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcatca < 2:3506841/76‑1 (MQ=255) cgcCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAaca > 2:5619315/1‑76 (MQ=255) cgcCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAaca > 1:3939473/1‑76 (MQ=255) cgcCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAaca > 1:3836730/1‑76 (MQ=255) cgcCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAaca < 1:2347797/76‑1 (MQ=255) gcCACTTGCATCATTTGTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCGCTCGCAGCTCATCAacac < 2:2158300/76‑1 (MQ=255) aGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACggg < 2:677846/76‑1 (MQ=255) gTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGt > 2:9403910/1‑76 (MQ=255) gTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGt > 2:4377221/1‑76 (MQ=255) ttGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGtt < 2:1052725/76‑1 (MQ=255) tGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTc > 1:7244263/1‑76 (MQ=255) tGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTc > 1:9337948/1‑76 (MQ=255) aTAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTg < 2:7848142/76‑1 (MQ=255) aTAATTTCTGCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTg < 1:1393616/76‑1 (MQ=255) tAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGa > 2:8916745/1‑76 (MQ=255) tAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGa > 2:9208410/1‑76 (MQ=255) tAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGa > 2:5503172/1‑76 (MQ=255) aaTTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGAc > 2:3138563/1‑76 (MQ=255) aaTTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGAGCTCTCGCAGCTCATCAACACGGGATCGTGAc > 2:8138299/1‑76 (MQ=255) ttcttcGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCCGCTCATCAACACGGGTTCGTGACGtc > 1:5723087/1‑76 (MQ=255) ttcttcGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGtc > 2:6335441/1‑76 (MQ=255) ttcgttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCa < 1:4716433/76‑1 (MQ=255) ttcgttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCa > 1:4685494/1‑76 (MQ=255) tcgttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCaa > 1:6811892/1‑76 (MQ=255) tcgttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCaa < 1:1322442/76‑1 (MQ=255) gttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATc < 1:5642664/76‑1 (MQ=255) ttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATca > 2:7605454/1‑76 (MQ=255) ttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATca < 2:644343/76‑1 (MQ=255) ttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGGCTCAATca > 1:2196037/1‑76 (MQ=255) tcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcac < 2:3075403/76‑1 (MQ=255) cgccgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcatc < 2:5992058/48‑1 (MQ=255) cgccgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcacc > 1:1198818/1‑76 (MQ=255) gccgcATTGCCCAATACTGCGCTGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcacca < 1:8138299/76‑1 (MQ=255) gccgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcacca < 1:3138563/76‑1 (MQ=255) gccgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcacca < 2:4043522/76‑1 (MQ=255) gccgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcacca < 1:6980470/76‑1 (MQ=255) ccgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcaccac < 2:3699886/76‑1 (MQ=255) gcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCAccacca > 2:7224594/1‑76 (MQ=255) cATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAg > 2:1924505/1‑76 (MQ=255) aTTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGc > 2:4630871/1‑76 (MQ=255) tGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCgg > 2:4108368/1‑76 (MQ=255) tGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCgg > 2:7478784/1‑76 (MQ=255) tGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCgg > 1:7946054/1‑76 (MQ=255) tGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCgg > 2:6172631/1‑76 (MQ=255) gCCCAATACTGCGCGGGCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGa < 1:5096598/76‑1 (MQ=255) gCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGa > 2:7115757/1‑76 (MQ=255) ccAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAat > 2:2831031/1‑76 (MQ=255) cAATACTGCGCGTCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcacc < 1:4481956/64‑1 (MQ=255) cAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcacc > 2:2515853/1‑64 (MQ=255) atttACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAata > 2:14698/4‑76 (MQ=255) atttACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAata > 2:4983273/4‑76 (MQ=255) atttACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAata > 2:5407623/4‑76 (MQ=255) aaTACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAatat > 2:66395/1‑76 (MQ=255) aaTACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAatat > 2:6538447/1‑76 (MQ=255) aaTACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAatat > 2:9312419/1‑76 (MQ=255) aaTACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAatat > 2:9419810/1‑76 (MQ=255) aaTACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAatat > 2:4226180/1‑76 (MQ=255) aCTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCag < 1:7165933/76‑1 (MQ=255) aCTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCag > 1:2638409/1‑76 (MQ=255) cTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCaga > 2:9141585/1‑76 (MQ=255) cTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCaga > 1:8740465/1‑76 (MQ=255) tGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCagag > 2:2699840/1‑76 (MQ=255) tGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCagag > 2:893468/1‑76 (MQ=255) gcgcGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAgg > 2:7552269/1‑76 (MQ=255) gcgcGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAgg > 2:3301808/1‑76 (MQ=255) gcgcGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAgg > 2:2073816/1‑76 (MQ=255) gcgcGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAgg < 1:3180286/76‑1 (MQ=255) gcgcGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAgg > 2:9489247/1‑76 (MQ=255) cgGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGa > 2:1358499/1‑76 (MQ=255) | AGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGA > REL606/473852‑473996 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |