| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 649,079 | T→C | T575A (ACC→GCC) | mrdA ← | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 649,079 | 0 | T | C | 96.7% | 671.1 / 7.3 | 180 | T575A (ACC→GCC) | mrdA | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base C (86/87); total (87/93) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01 | |||||||||||
Only 100 of 220 total aligned reads displayed.
CGGACCCGCGCCGCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAA > REL606/649008‑649151 | cgGACCCGCGCCGCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGTGGCAAAGGCGGccatc > 2:3796172/1‑76 (MQ=255)cgGACCCGCGCCGCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCCAAAGGGGccatc > 2:1048540/1‑76 (MQ=255)cgGACCCGCGCCGCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAATCGGgcata > 2:1338935/1‑75 (MQ=255) ccgccccgccTTCCTCCAGAATCATGGCGCCAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGttt < 2:7323705/76‑1 (MQ=255) ccGCGCCGCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTTGATGGCGCAAAAGCGGGCCTCCGttt > 2:123065/1‑76 (MQ=255) ccGCGCCGCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGGGCAAAGGCGGCCATCAGttt > 2:8555591/1‑76 (MQ=255) ccGCGCCGCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCCTCAGgtt > 2:8403570/1‑76 (MQ=255) ccGCGCCGCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGttt > 2:9258769/1‑76 (MQ=255) ccGCGCCGCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGttt > 2:9034024/1‑76 (MQ=255) ccGCGCCGCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGttt > 2:7103039/1‑76 (MQ=255) ccGCGCCGCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGttt > 2:2693992/1‑76 (MQ=255) ccccgccgTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTtgtg < 2:1196497/74‑1 (MQ=255) gccgccGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTTTgg > 2:4456475/1‑76 (MQ=255) gccgccGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTgg > 2:3124353/1‑76 (MQ=255) gccgccGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAAGCGGCCATCAGTTTGTgg > 2:2577504/1‑76 (MQ=255) cccgcctTTCTCCAGAATCATGGCCCCAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTgg < 2:9361586/75‑1 (MQ=255) cccgccgTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTgg < 1:2039459/75‑1 (MQ=255) ccgccgTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGGTTGTGGt > 1:8300180/1‑76 (MQ=255) ccgccgTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGGTTGTGGt > 1:6151546/1‑76 (MQ=255) cgccgTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTc > 2:9568487/1‑76 (MQ=255) cgccgTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTc < 1:3013504/76‑1 (MQ=255) cgccgTTCCCCAGAATCAGGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTc < 1:4903655/76‑1 (MQ=255) cgTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGGCATCAGTTTGTGGTCACg > 1:8251200/1‑76 (MQ=255) cgTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGGCACg > 1:4019746/1‑76 (MQ=255) gtactcCAGAAAAAGGGGGGCAGCTACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGt < 1:7599312/73‑1 (MQ=255) gTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCCGTTTGTGGTCACGt > 2:3373856/1‑76 (MQ=255) gTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGt < 1:1363017/76‑1 (MQ=255) ttCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGGCACGTa > 2:2496968/1‑76 (MQ=255) tctcCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTaa < 1:492122/76‑1 (MQ=255) ctcCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTaaa < 2:2348152/76‑1 (MQ=255) tcCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAAc < 1:1048540/76‑1 (MQ=255) tcCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAAc < 1:1338935/76‑1 (MQ=255) cccAGAATCTGGGCCACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAAc < 1:9208914/75‑1 (MQ=255) cccAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAAc < 1:2358531/75‑1 (MQ=255) cccAGAATCATGCCGCCAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAAc < 1:3796172/75‑1 (MQ=255) aGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCt > 2:6974889/1‑76 (MQ=255) aGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCt < 1:186332/76‑1 (MQ=255) aGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGGAAACGCt > 2:8306940/1‑76 (MQ=255) aaTCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCg < 1:4701069/76‑1 (MQ=255) tCATGGCGACAGCCACTTGCGGGTTGTTGTATGGCGCAAAGGCGGCCATCAGCTTGTGGTCACGTAAAAGCTCggg > 2:7776323/1‑75 (MQ=255) tCATGGCAACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGc < 2:9524900/76‑1 (MQ=255) cATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCa > 1:2231114/1‑76 (MQ=255) cATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCAGCa > 1:2835902/1‑76 (MQ=255) ggCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCCTCAGTTTGTGGTCACGTAAACGCTCGGGAAtt > 2:5302298/1‑76 (MQ=255) ggCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCCTCAGTTTGTGGTCACGTAAACGCTCGGCAAtt > 1:5489093/1‑76 (MQ=255) ggCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAAtt > 1:2580011/1‑76 (MQ=255) gcgcCAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAAttt < 1:1842821/76‑1 (MQ=255) gcgcCAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAAttt < 2:6923387/76‑1 (MQ=255) aCAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTtgt > 2:5755278/1‑76 (MQ=255) aGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCCTCAGTTTGTGGTCACGGAAACGCTCGGCAATTTTGTgc > 2:6482063/1‑76 (MQ=255) gCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTgcg < 2:5886454/76‑1 (MQ=255) gCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTgcg < 1:5767731/76‑1 (MQ=255) cTTGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCAtt < 1:3452167/76‑1 (MQ=255) ttGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATta > 2:2088898/1‑76 (MQ=255) ttGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATta > 2:1500420/1‑76 (MQ=255) ttGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATta > 2:2946679/1‑76 (MQ=255) ttGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATta < 1:932641/76‑1 (MQ=255) ttGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATta < 1:8916690/76‑1 (MQ=255) tGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATtat < 1:8623020/76‑1 (MQ=255) tGCGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATtat < 2:6194791/76‑1 (MQ=255) cGGATTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGGGCATTATAg < 2:406898/76‑1 (MQ=255) aTTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGtt < 1:288670/76‑1 (MQ=255) aTTGTTGTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGtt > 1:2173779/1‑76 (MQ=255) ttgttgTATGGCGCAAAGGCGGTCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGttt < 1:1971200/76‑1 (MQ=255) ttgttgTATGGCGCAAAGGCGGTCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGttt < 1:3124050/76‑1 (MQ=255) ttgttgTATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGttt > 2:6571632/1‑76 (MQ=255) tgttgtATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTttc < 2:5807809/76‑1 (MQ=255) gttgtATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTttcg > 1:1670716/1‑76 (MQ=255) gttgtATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTttcg > 1:6039371/1‑76 (MQ=255) gttgtATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTttcg > 1:9140066/1‑76 (MQ=255) gttgtATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTttcg < 2:3695909/76‑1 (MQ=255) ttgtATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTttcgt > 2:2607594/1‑76 (MQ=255) gtATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTttcgttc > 2:6102365/1‑76 (MQ=255) tATGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTttcgttcg > 2:1858534/1‑76 (MQ=255) aTGGCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGc < 2:7640993/76‑1 (MQ=255) ggCGCAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCtt < 2:5119089/76‑1 (MQ=255) cgcAAAGGCGTCCATCAGGTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTc < 2:3907001/76‑1 (MQ=255) cgcAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTc < 2:1670716/76‑1 (MQ=255) cgcAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTc < 1:7309090/76‑1 (MQ=255) cAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAg > 1:4584860/1‑76 (MQ=255) cAAAGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAg < 1:1889168/76‑1 (MQ=255) aaaGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGa > 2:4717955/1‑76 (MQ=255) aaaGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGa > 2:1766029/1‑76 (MQ=255) aaaGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGa > 2:4206278/1‑76 (MQ=255) aaGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTGGTTCGCTTTCAGAc > 1:6571246/1‑76 (MQ=255) aaGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGAc < 1:3918606/76‑1 (MQ=255) aaGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGAc > 1:5584187/1‑76 (MQ=255) aaGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGAc < 1:4119866/76‑1 (MQ=255) aaGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGAc < 1:8462539/76‑1 (MQ=255) aGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGAcc > 1:4734662/1‑76 (MQ=255) aGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGAcc > 1:9525750/1‑76 (MQ=255) aGGCGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGAcc > 2:6443314/1‑76 (MQ=255) ggcggcCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCg < 1:9566323/76‑1 (MQ=255) ggcggcCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCg > 2:6785833/1‑76 (MQ=255) ggcggcCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCg < 1:155378/76‑1 (MQ=255) ggcggcCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCg > 2:2840814/1‑76 (MQ=255) cGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGaa > 1:7460332/1‑76 (MQ=255) cGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGaa > 1:6969842/1‑76 (MQ=255) cGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGaa > 1:1284156/1‑76 (MQ=255) cGGCCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGaa > 1:3660884/1‑76 (MQ=255) | CGGACCCGCGCCGCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAA > REL606/649008‑649151 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |