| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,504,889 | (A)8→7 | intergenic (+633/‑92) | insB‑25 → / → ECB_04162 | b4563(b4576); IS1 protein insB/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,504,882 | 0 | A | . | 96.6% | 1281.0 / 34.0 | 268 | intergenic (+626/‑99) | insB‑25/ECB_04162 | b4563(b4576); IS1 protein insB/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (4/5); new base . (109/150); total (113/155) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
Only 100 of 301 total aligned reads displayed.
AGATAATGTTTTTAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCACAAAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTTATATAGCAAAATA > REL606/4504807‑4504953 | aGATAATGTTTTTAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTcaca > 1:2188520/1‑76 (MQ=255) aTAATGTTTTTAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCACaaa > 1:3181787/1‑76 (MQ=255) tAATGTTTTTAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCACaaaa > 1:10210062/1‑76 (MQ=255) tAATGTTTTTAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCACaaaa > 1:4400823/1‑76 (MQ=255) tAATGTTTTTAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCACaaaa > 2:8061263/1‑76 (MQ=255) aTGTTTTTAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCACaaaaaa < 1:8502505/76‑1 (MQ=255) tGTTTTTAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCACaaaaaaa < 1:3655824/76‑1 (MQ=255) ttAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCAtt < 1:7650953/76‑1 (MQ=255) ttAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCAtt < 1:2590136/76‑1 (MQ=255) tAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTa < 1:106591/76‑1 (MQ=255) tAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTa < 2:5221508/76‑1 (MQ=255) aGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAg > 2:1538/1‑76 (MQ=255) aGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAg > 2:3062388/1‑76 (MQ=255) aGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAg > 2:8773589/1‑76 (MQ=255) gTAAAAAAGAGTTGATTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGt > 1:7452201/1‑76 (MQ=255) aaaaaaGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCt > 1:5727507/1‑76 (MQ=255) aaaaaGAGTTGACTGAATATAATTCAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTg < 2:7725504/76‑1 (MQ=255) aaaaaGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTg < 2:9097371/76‑1 (MQ=255) aaaaaGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTg < 2:4932219/76‑1 (MQ=255) aaaGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCACAAAAAAAACCATTAGTCTGc > 2:5056190/1‑76 (MQ=255) aaGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCtt < 1:8529377/76‑1 (MQ=255) aaGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCtt > 2:5277348/1‑76 (MQ=255) agTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCt < 2:2671170/76‑1 (MQ=255) ttGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGt > 2:8473012/1‑76 (MQ=255) gACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTc > 1:9471348/1‑76 (MQ=255) gACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTc > 1:5938197/1‑76 (MQ=255) gACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTc > 2:5259702/1‑76 (MQ=255) cTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCACAAAAAAAACCATTAGTCTGCTTCCTGTTCt < 2:6122936/76‑1 (MQ=255) cTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCtt < 1:7199989/76‑1 (MQ=255) tGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTa > 1:7088092/1‑76 (MQ=255) tGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTa < 2:6884183/76‑1 (MQ=255) tGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTa < 1:1981426/76‑1 (MQ=255) gAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTAc < 2:9307745/76‑1 (MQ=255) gAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTAc < 1:13558/76‑1 (MQ=255) aaTATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACa > 2:5975451/1‑76 (MQ=255) aaTATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACa > 1:7342190/1‑76 (MQ=255) aaTATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACa < 1:10008348/76‑1 (MQ=255) atatAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACaa < 1:9909062/76‑1 (MQ=255) atatAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACaa < 2:3559112/76‑1 (MQ=255) ataataCAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACaata < 1:1606994/76‑1 (MQ=255) ataataCAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACaata > 1:2950550/1‑76 (MQ=255) taataCAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACaataa < 1:795611/76‑1 (MQ=255) taataCAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACaataa < 2:455176/76‑1 (MQ=255) taataCAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACaataa > 1:7759771/1‑76 (MQ=255) taataCAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACaataa > 1:3420593/1‑76 (MQ=255) aataCAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACaataat > 1:5450222/1‑76 (MQ=255) ataCAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATc < 2:4622523/76‑1 (MQ=255) ataCAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATc < 2:9539667/76‑1 (MQ=255) ataCAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATc > 2:3206576/1‑76 (MQ=255) taCAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCa < 2:10091009/76‑1 (MQ=255) taCAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCa < 1:8908268/76‑1 (MQ=255) aCAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAg < 2:7158616/76‑1 (MQ=255) cAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAACCCATTAGTCTGCTTCCTGTTCTTACAATAATCAGt > 2:10215757/1‑76 (MQ=255) cAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGt < 1:6015779/76‑1 (MQ=255) ccTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGtatt < 2:3899253/76‑1 (MQ=255) ccTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGtatt < 1:2363544/76‑1 (MQ=255) ccTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGtatt < 1:9994929/76‑1 (MQ=255) ctctTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGtatta < 2:4458334/76‑1 (MQ=255) tctTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGtattat > 2:1456650/1‑76 (MQ=255) tctTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGtattat > 2:5733557/1‑76 (MQ=255) ctTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATg < 2:7604936/76‑1 (MQ=255) ttCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGt > 1:6627021/1‑76 (MQ=255) ccTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtat > 2:127744/1‑76 (MQ=255) ccTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtat > 1:6195442/1‑76 (MQ=255) cTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtatt > 2:6721500/1‑76 (MQ=255) tACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtattt < 2:2472103/76‑1 (MQ=255) tACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtattt > 2:171682/1‑76 (MQ=255) aCATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtatttt < 2:4300142/76‑1 (MQ=255) aCATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtatttt < 2:1940562/76‑1 (MQ=255) aCATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtatttt < 1:8618828/76‑1 (MQ=255) aCATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtatttt > 1:4131327/1‑76 (MQ=255) aCATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtatttt > 1:9661282/1‑76 (MQ=255) cATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtatttta < 2:6989199/76‑1 (MQ=255) aTAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtattttat < 2:9463441/76‑1 (MQ=255) aTAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtattttat > 1:3832401/1‑76 (MQ=255) aTAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtattttat < 2:5503743/76‑1 (MQ=255) tAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtattttatt < 1:4636259/76‑1 (MQ=255) aagaagGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtattttattt > 2:1575424/1‑76 (MQ=255) gaagGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTttatttat < 1:1137363/76‑1 (MQ=255) aagGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtattttatt < 1:5289245/72‑1 (MQ=255) aagGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTtata > 2:6455164/1‑76 (MQ=255) aagGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTtata > 2:7997721/1‑76 (MQ=255) agGAATTTGCTCAC‑ATAAAATCGATTAGTTTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTGTTtatat > 2:3732496/1‑76 (MQ=255) agGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTtatat < 2:4125943/76‑1 (MQ=255) agGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTtatat < 1:3442376/76‑1 (MQ=255) agGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTtatat > 2:4764451/1‑76 (MQ=255) ggAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTtatata < 1:4813591/76‑1 (MQ=255) ggAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTtatata < 2:9758285/76‑1 (MQ=255) gAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTTATATAg < 2:7248705/76‑1 (MQ=255) gAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTTATATAg < 1:5036202/76‑1 (MQ=255) aaTGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTTATATAGc > 1:6156193/1‑76 (MQ=255) aaTGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTTATATAGc < 2:6168648/76‑1 (MQ=255) aaTGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTTATATAGc < 1:9839721/76‑1 (MQ=255) aTGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTTATATAGCa < 1:3027173/76‑1 (MQ=255) tgtgCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTTATATAGCaa < 1:9156571/76‑1 (MQ=255) tgtgCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTTATATAGCaa < 2:2188520/76‑1 (MQ=255) gtgCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTTATATAGCaaa < 1:499214/76‑1 (MQ=255) gtgCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTTATATAGCaaa < 1:6951249/76‑1 (MQ=255) gtgCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTTATATAGCaaa > 2:3462519/1‑76 (MQ=255) tgCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTTATATAGCaaaa > 2:8533762/1‑76 (MQ=255) | AGATAATGTTTTTAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCACAAAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTTATATAGCAAAATA > REL606/4504807‑4504953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |