| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,297,789 | Δ1 bp | coding (1294/1359 nt) | glpT ← | sn‑glycerol‑3‑phosphate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,297,789 | 0 | T | . | 98.4% | 1203.9 / 10.1 | 245 | coding (1294/1359 nt) | glpT | sn‑glycerol‑3‑phosphate transporter |
| Reads supporting (aligned to +/- strand): ref base T (1/3); new base . (138/103); total (139/106) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 302 total aligned reads displayed.
ATGCCATTAGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAATCAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCGAAGAAGTCC > REL606/2297718‑2297859 | aTGCCATTAGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCacaacaa‑caaca < 2:1089710/76‑1 (MQ=255)aTGCCATTAGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCacaacaa‑caaca > 1:1187164/1‑76 (MQ=255)aTGCCATTAGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCacaacaa‑caaca < 1:4462489/76‑1 (MQ=255)aTGCCATTAGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCacaacaa‑caaca < 1:4731950/76‑1 (MQ=255) gCCATTAGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAg > 1:526713/1‑76 (MQ=255) ccATTAGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGa < 1:9767505/76‑1 (MQ=255) ccATTAGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGa < 1:6866041/76‑1 (MQ=255) cATTAGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGAt < 2:2924154/76‑1 (MQ=255) cATTAGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGAt < 1:8487744/76‑1 (MQ=255) cATTAGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGAt > 1:892647/1‑76 (MQ=255) cATTAGCCTCCGTTGCGTTTTTGCAGTAATTGATCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGAt < 1:541395/76‑1 (MQ=255) aTTAGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATa < 1:2197670/76‑1 (MQ=255) aTTAGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATa < 1:9646838/76‑1 (MQ=255) aTTAGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATa < 1:4065012/76‑1 (MQ=255) ttAGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAATCAACAAGATa < 1:4643709/76‑1 (MQ=255) ttAGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATaa < 1:6411765/76‑1 (MQ=255) ttAGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATaa < 2:3984679/76‑1 (MQ=255) ttAGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATaa < 1:948017/76‑1 (MQ=255) tAGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGCCGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAAc < 1:5340816/76‑1 (MQ=255) tAGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAAc < 1:785916/76‑1 (MQ=255) tAGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAAc > 1:8595653/1‑76 (MQ=255) aGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAAcc < 2:4117443/76‑1 (MQ=255) gCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCg > 1:3291755/1‑76 (MQ=255) ccTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGc > 1:3613560/1‑76 (MQ=255) cTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGcc < 1:5544732/76‑1 (MQ=255) tCCGTTGCGTTTTTTGATTAATTTGTCCAGGCGACTTTTTTCGCCAATCATCACAACAA‑AAACAAGATAAGCGCCa < 2:2534956/76‑1 (MQ=255) tCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCa > 2:9878048/1‑76 (MQ=255) tCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCa < 2:1295594/76‑1 (MQ=255) tCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCa > 1:1979938/1‑76 (MQ=255) ccGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAg < 1:2421657/76‑1 (MQ=255) cGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGa > 2:8967754/1‑76 (MQ=255) gTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGaa < 2:6119369/76‑1 (MQ=255) gTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGaa < 1:1662375/76‑1 (MQ=255) gTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGaa < 2:189700/76‑1 (MQ=255) gTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGaa > 2:2811406/1‑76 (MQ=255) ttGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAAt > 2:4978667/1‑76 (MQ=255) ttGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAAt > 1:1891966/1‑76 (MQ=255) ttGCGTTTGTGCAGTAATTGTTCATGGCGACGTTTGTCGCCAATCTTCACAACAA‑CAACAAGATAACCGCCAGAAt < 2:937160/76‑1 (MQ=255) tGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAAtg < 1:1777342/76‑1 (MQ=255) gCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAAtgc < 2:7449039/76‑1 (MQ=255) gCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAAtgc > 1:4967864/1‑76 (MQ=255) gTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAAtgctg < 2:6872027/76‑1 (MQ=255) gTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAAtgctg < 1:8138647/76‑1 (MQ=255) tttttGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAAtgctgc > 2:10088086/1‑76 (MQ=255) tttttGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAAtgctgc < 1:9090593/76‑1 (MQ=255) tttGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTgccg > 2:697877/1‑76 (MQ=255) ttGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTgccgc > 1:4204291/1‑76 (MQ=255) ttGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTgccgc < 1:7701380/76‑1 (MQ=255) tGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTgccgcc < 2:7766487/76‑1 (MQ=255) tGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTgccgcc > 2:6827308/1‑76 (MQ=255) cAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCaa > 1:6014236/1‑76 (MQ=255) gTAATTTTTTATGGGTACTTTTTCCGCCAATCATCACAACAA‑CACCAAAATAACCGCCAGAATGCTTCCGCCAATc < 2:2304294/76‑1 (MQ=255) gTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATc > 2:8731595/1‑76 (MQ=255) tAATTGTTCAGGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCa < 1:876091/76‑1 (MQ=255) ttttCAGGGCGCCTTTTTTCGCAAATCATCA‑AAAAAACAAAAAAAAACCCGCCAGAATGCTGCCGCCAATCATTAc < 2:3582048/74‑1 (MQ=255) tGTTCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTAc > 2:480194/1‑76 (MQ=255) tttCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTAcc < 1:9899813/75‑1 (MQ=255) tCATGGCGGCGTTTTTCGCAAATCATCACAACAA‑CAACAAGATACCCGCCAGAATGCTGCCGCCAATCATTACCAt < 2:2084536/76‑1 (MQ=255) tCATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCAt > 2:4366867/1‑76 (MQ=255) cATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATa > 2:6728690/1‑76 (MQ=255) cATGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATa > 1:10083362/1‑76 (MQ=255) gtGGCGAGTTTTTTCCCCAATCACCACAACAA‑CAAAAACAAAACCGCCGGAATGCTGCCGCCAATCATTACCATaa < 2:7239781/75‑1 (MQ=255) agggCGCCGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATaa < 1:9870199/74‑1 (MQ=255) aTGGCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATaa < 1:10136506/76‑1 (MQ=255) tGGCGCCGTTTTTCGCAAATCACCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATaaa < 1:1910893/76‑1 (MQ=255) ggCGACTTTTTTCGCCAATCATCACAACAA‑CAAAAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAg < 2:2481466/76‑1 (MQ=255) ggCGACTTTTTTCGCAAATCATCAAAACAA‑CAAAAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAg < 1:3020673/76‑1 (MQ=255) ggCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAg < 1:3423620/76‑1 (MQ=255) ggCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAg < 1:2569235/76‑1 (MQ=255) gCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAgc < 1:3826904/76‑1 (MQ=255) gCGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAgc > 1:4272107/1‑76 (MQ=255) cGACTTTTTCCCCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAgcc < 1:6545133/76‑1 (MQ=255) cGACGTTTTTCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCCCCAATCATTACCATAAAgcc < 2:2614499/76‑1 (MQ=255) gcggTTTTCCGCAAATCAACACAACAA‑CAAAAAGATACCCGCCAGAATGCTGCCGCCAATCATTACCATAAAgccg < 1:6244595/73‑1 (MQ=255) gcGTTTTTCCCCAAACAACCAAAAAAAAAAAAAAAAACCCGCAAGAATGCGGCCGCCAATCATTACCATAAAgccg < 2:5415919/75‑1 (MQ=255) cGTTTTCCCCCAATCATCAAAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAgccgcc < 1:1353175/76‑1 (MQ=255) tttttCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCAt > 2:328462/1‑76 (MQ=255) ttttCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATc > 1:159053/1‑76 (MQ=255) tttCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATcc > 1:10386946/1‑76 (MQ=255) tccGCAAACCATCACAA‑AAACAACAAGATACCCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATccc < 1:697877/74‑1 (MQ=255) tCGCCAATCATCACAACAA‑CAACAAGATACCCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCa < 1:8976600/76‑1 (MQ=255) tCGCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCa > 1:2664270/1‑76 (MQ=255) tCGCCAATCATCACAACAA‑CAAAAAAATACCCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCa < 1:8334477/76‑1 (MQ=255) tCGCAAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCa < 1:3163115/76‑1 (MQ=255) cccccAACCACCAAAACAA‑CAAAAAAAAACCCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCa < 2:925154/73‑1 (MQ=255) gCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGc > 2:605448/1‑76 (MQ=255) gCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGc > 2:9432433/1‑76 (MQ=255) gCCAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATCACCATAAAGCCGCCATCCCAGc > 2:7636458/1‑76 (MQ=255) cAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCg > 1:4675396/1‑76 (MQ=255) cAATCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCg > 1:2819654/1‑76 (MQ=255) aaTCATCACAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCga > 1:5786236/1‑76 (MQ=255) catcaCAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCgaaga > 1:1504618/1‑76 (MQ=255) atcaCAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCgaagaa > 1:5205584/1‑76 (MQ=255) tcaCAACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCGaagaag > 1:7106311/1‑76 (MQ=255) acacaACAA‑CAACAAGAACCCCCCAAGAATGCTGCCGCCAATCATTACAAAAAAGCCGCCATCCCAGCCGaagaag < 1:1550114/75‑1 (MQ=255) cacaACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCGAAGAAGt > 1:7314615/1‑76 (MQ=255) cacaACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCGAAGAAGt > 2:8467665/1‑76 (MQ=255) cacaACAA‑CAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCGAAGAAGt > 1:6697922/1‑76 (MQ=255) caacaa‑caacaaGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCGAAGAAGTcc > 2:8220383/1‑76 (MQ=255) caacaa‑caacaaGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCGAAGAAGTcc > 2:3886304/1‑76 (MQ=255) | ATGCCATTAGCCTCCGTTGCGTTTTTGCAGTAATTGTTCATGGCGACGTTTTTCGCCAATCATCACAACAATCAACAAGATAACCGCCAGAATGCTGCCGCCAATCATTACCATAAAGCCGCCATCCCAGCCGAAGAAGTCC > REL606/2297718‑2297859 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |