| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,875,625 | 1 | . | T | 99.2% | 1010.6 / ‑0.2 | 244 | intergenic (‑59/+294) | glmU/atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base T (92/150); total (93/151) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.36e-01 | |||||||||||
Only 100 of 294 total aligned reads displayed.
ATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCC > REL606/3875550‑3875695 | aGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tt < 1:10413277/76‑1 (MQ=255) aGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tt > 2:6167216/1‑76 (MQ=255) gCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt > 2:5640878/1‑76 (MQ=255) cATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t > 1:6013548/1‑76 (MQ=255) cATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t > 1:9609362/1‑76 (MQ=255) attattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t‑t < 1:4055925/76‑1 (MQ=255) attattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t‑t < 1:6571098/76‑1 (MQ=255) attattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t‑t < 1:978287/76‑1 (MQ=255) ttattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t‑t‑t > 2:69636/1‑76 (MQ=255) tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t‑t‑tt > 1:6357123/1‑76 (MQ=255) tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t‑t‑tt > 2:5514527/1‑76 (MQ=255) tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t‑t‑tt > 1:1168278/1‑76 (MQ=255) tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t‑t‑tt > 2:454080/1‑76 (MQ=255) attCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t‑t‑ttt < 2:5239783/76‑2 (MQ=255) tCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t‑t‑tttca > 2:9104628/1‑73 (MQ=255) cATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGt < 2:4786439/76‑1 (MQ=255) cATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGt > 2:3182469/1‑76 (MQ=255) cATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGt < 2:1207176/76‑1 (MQ=255) aTACGCGTCCTGACTGTAATTTGAGAACGACTTTAAACCGCTTCACCTTGCAAAAACTACATTTT‑T‑T‑TTCATCGTg > 1:7479978/1‑76 (MQ=255) aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTg > 2:7244359/1‑76 (MQ=255) tACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGa < 1:5936167/76‑1 (MQ=255) aCGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGaa < 1:5480784/76‑1 (MQ=255) aCGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGaa > 1:3654894/1‑76 (MQ=255) cgcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGaaa < 1:4283006/76‑1 (MQ=255) cgcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGaaa < 2:4314908/76‑1 (MQ=255) gcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAAt < 1:7140548/76‑1 (MQ=255) cgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATg > 2:3527712/1‑76 (MQ=255) gTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATgg > 2:4081149/1‑76 (MQ=255) gTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATgg < 2:7877368/76‑1 (MQ=255) gTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATgg < 1:1481699/76‑1 (MQ=255) tCCTGACTGTAATTTGAGAACGCATTTAACCCGCTTGACCTTGACAAAACTCCC‑TTT‑TGT‑TTCACCGCGAAACggc > 2:4471435/1‑75 (MQ=255) tCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGa < 1:2419200/76‑1 (MQ=255) tCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGa > 1:2744231/1‑76 (MQ=255) ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGAc < 2:6247381/76‑1 (MQ=255) cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACa > 1:6476016/1‑76 (MQ=255) tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACag < 2:185289/76‑1 (MQ=255) tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACag < 1:1604620/76‑1 (MQ=255) tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACag < 1:256078/76‑1 (MQ=255) cTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAgg > 2:3408013/1‑76 (MQ=255) cTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTTCT‑T‑TTCATCGTGAAATGGACAGAgg > 2:9252263/1‑76 (MQ=255) tGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGa > 1:4587015/1‑76 (MQ=255) tGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGa > 2:8177451/1‑76 (MQ=255) tGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGa < 2:8974893/76‑1 (MQ=255) tAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATa > 1:10287152/1‑76 (MQ=255) tAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATa < 1:9480031/76‑1 (MQ=255) tAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATa < 1:3740391/76‑1 (MQ=255) tAATTTGAGAACGAAGTTAAAACGATTAACCTAGAAAAATCTACA‑TTT‑T‑TATTCATCGTGAAATGAACAGAGGAtt < 2:7701587/76‑2 (MQ=255) aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATaa < 2:282017/76‑1 (MQ=255) aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATaa > 2:2690739/1‑76 (MQ=255) aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATaa < 1:725897/76‑1 (MQ=255) aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATaa > 1:4769033/1‑76 (MQ=255) aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATaaa < 2:2139223/76‑1 (MQ=255) aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATaaa < 2:3695944/76‑1 (MQ=255) aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATaaa > 1:9457578/1‑76 (MQ=255) aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATaaa > 1:10066411/1‑76 (MQ=255) aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATaaa < 1:3219951/76‑1 (MQ=255) aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATaaa < 1:5128445/76‑1 (MQ=255) tttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAAt < 2:2934537/76‑1 (MQ=255) tttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAAt > 1:6109375/1‑76 (MQ=255) tttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAAt > 1:393814/1‑76 (MQ=255) ttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAAtt < 1:822230/76‑1 (MQ=255) tGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACa > 2:8820499/1‑64 (MQ=255) tGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTg < 2:10165822/76‑1 (MQ=255) tGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTg < 2:7035716/76‑1 (MQ=255) tGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGt < 1:454080/76‑1 (MQ=255) gagaACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGt < 1:5514527/76‑1 (MQ=255) gaACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTc < 1:3645992/76‑1 (MQ=255) gaACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTc < 2:7560156/76‑1 (MQ=255) aaCGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCa < 1:2287859/76‑1 (MQ=255) aCGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCaa < 2:334837/76‑1 (MQ=255) cGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAAt < 1:9237484/76‑1 (MQ=255) cGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAAt < 1:4345248/76‑1 (MQ=255) gAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATa < 1:1355998/76‑1 (MQ=255) aaTTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa < 2:4194806/76‑1 (MQ=255) aaTTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa < 2:9329051/76‑1 (MQ=255) tttAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACg > 1:8863510/1‑76 (MQ=255) ttAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACgg > 1:5209125/1‑76 (MQ=255) tAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa > 1:5267141/1‑76 (MQ=255) tAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa < 1:7382229/76‑1 (MQ=255) aaaCCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAt < 1:1001715/76‑1 (MQ=255) aaCCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt < 1:8602736/76‑1 (MQ=255) aCCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTa < 2:10310410/76‑1 (MQ=255) ccGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt > 1:7647487/1‑76 (MQ=255) ccGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt < 1:6943555/76‑1 (MQ=255) tCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCg > 2:1480008/1‑76 (MQ=255) aCCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCt > 2:5911457/1‑76 (MQ=255) ttGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGAc < 1:8735555/76‑1 (MQ=255) ttGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGAc < 1:8996030/76‑1 (MQ=255) ttGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGAc < 1:1944207/76‑1 (MQ=255) tGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg < 1:8358406/76‑1 (MQ=255) tGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg < 1:9357934/76‑1 (MQ=255) tGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg < 1:9972811/76‑1 (MQ=255) tGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg < 1:294509/76‑1 (MQ=255) aaaaaaCTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaa < 1:7922833/76‑1 (MQ=255) aaaaaaCTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaa < 1:3018306/76‑1 (MQ=255) aaaaaCTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa < 2:6653174/76‑1 (MQ=255) aaaaaCTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa > 2:5386323/1‑76 (MQ=255) aaaaaCTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa < 2:8432852/76‑1 (MQ=255) aaaaCTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg > 2:9215544/1‑76 (MQ=255) aCTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgcgc < 2:4816368/76‑1 (MQ=255) | ATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCC > REL606/3875550‑3875695 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |