Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 4,097,495 +CC coding (78/846 nt) glpF ← glycerol facilitator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6064,097,4921.C89.4% 932.0 / 98.2 245coding (81/846 nt)glpFglycerol facilitator
Reads supporting (aligned to +/- strand):  ref base . (11/15);  new base C (126/93);  total (137/108)
Fisher's exact test for biased strand distribution p-value = 1.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*REL6064,097,4922.C89.4% 934.0 / 98.2 245coding (81/846 nt)glpFglycerol facilitator
Reads supporting (aligned to +/- strand):  ref base . (11/15);  new base C (126/93);  total (137/108)
Fisher's exact test for biased strand distribution p-value = 1.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 270 total aligned reads displayed.

ACCCCCAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑A‑‑CCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGCACTGGCCTTTCAAGGTT  >  REL606/4097418‑4097558
                                                                             ||                                                                  
aCCCCCAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTgcaacgca‑‑a‑‑c                                                                   >  2:785046/1‑76 (MQ=255)
  ccccAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑A‑‑ccc                                                                 >  2:4709546/1‑76 (MQ=255)
   cccAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑A‑‑cccc                                                                <  1:6262998/76‑2 (MQ=255)
   cccAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑A‑‑cccc                                                                <  1:1804329/76‑2 (MQ=255)
   cccAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑A‑‑cccc                                                                >  2:3334615/1‑75 (MQ=255)
    ccAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑A‑‑ccccc                                                               >  1:6784960/1‑74 (MQ=255)
    ccAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑A‑‑ccccc                                                               <  1:9077141/76‑3 (MQ=255)
    ccAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑A‑‑ccccc                                                               >  1:9161236/1‑74 (MQ=255)
     cAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑A‑‑CCccca                                                              <  1:1350526/76‑1 (MQ=255)
     cAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑A‑‑CCccca                                                              <  1:4504012/76‑1 (MQ=255)
      cgTCCCCAAAGGACACTGATTTCCCCCTGACCAAAAGCCGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑A‑‑CCCccac                                                             <  1:8016340/75‑1 (MQ=255)
      aGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑A‑‑CCCccac                                                             <  1:3106491/76‑1 (MQ=255)
      aGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑A‑‑CCCccac                                                             >  2:3853776/1‑76 (MQ=255)
       gTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑A‑‑CCCccaca                                                            <  1:10395582/76‑2 (MQ=255)
       gTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑A‑‑CCCccaca                                                            <  2:2711587/76‑2 (MQ=255)
        tCCCCATATGTCACTGATTTCCCACTGACAAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑A‑‑CCCccacac                                                           <  2:5997376/76‑3 (MQ=255)
        tCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑A‑‑CCCccacac                                                           >  2:4217052/1‑74 (MQ=255)
        tCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑A‑‑CCCccacac                                                           >  2:2529289/1‑74 (MQ=255)
        tCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑A‑‑CCCccacac                                                           <  1:8154352/76‑3 (MQ=255)
         ccccAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑A‑‑CCCccacaca                                                          <  2:4108889/76‑4 (MQ=255)
             aaaTGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCgaag                                                        >  2:4683562/1‑76 (MQ=255)
             aaaTGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCgaag                                                        >  2:10240176/1‑76 (MQ=255)
              aaTGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCgaaga                                                       >  2:613958/1‑76 (MQ=255)
               aTGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCgaagaa                                                      <  2:4578367/76‑1 (MQ=255)
               aTGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCgaagaa                                                      >  2:827669/1‑76 (MQ=255)
               aTGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCgaagaa                                                      >  1:5124222/1‑76 (MQ=255)
                tGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGaaa                                                     <  2:6529855/76‑1 (MQ=255)
                tGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGaaa                                                     >  1:1459257/1‑76 (MQ=255)
                tGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGaaa                                                     >  2:5084576/1‑76 (MQ=255)
                tGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGaaa                                                     >  2:9833922/1‑76 (MQ=255)
                 gACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGaaaa                                                    >  1:10226993/1‑76 (MQ=255)
                 gACACTGATTTCCCACTAACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGaaaa                                                    <  1:9450390/76‑1 (MQ=255)
                   cacTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATc                                                  >  2:1244456/1‑76 (MQ=255)
                   cacTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATc                                                  <  1:818474/76‑1 (MQ=255)
                    acTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATca                                                 <  1:2525062/76‑1 (MQ=255)
                    acTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATca                                                 <  2:5396071/76‑1 (MQ=255)
                    acTGATTTCCCACGGCCCAAAAGACGCCCCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATca                                                 <  1:2790194/76‑1 (MQ=255)
                     cTGATTCCCCACTGACCAAAAGACGCCCCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATcaa                                                <  2:8822790/76‑1 (MQ=255)
                      tGATTTCCCACTGACCAAAAGCCGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATcaac                                               <  2:1770555/76‑1 (MQ=255)
                      tGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATcaac                                               <  1:1272606/76‑1 (MQ=255)
                       taTTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACGCCGAAGAAAATcaaca                                              <  1:7587329/75‑1 (MQ=255)
                        aTTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATcaacaa                                             <  1:3894071/76‑1 (MQ=255)
                        aTTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATcaacaa                                             <  2:8526571/76‑1 (MQ=255)
                         tttCCCACTGACCAAAAGACGCCCCAGCGACTTTTAGTGCTGCAACGCAGCC‑‑CCCACACCGAAGAAAATCaacaac                                            <  1:1078003/76‑1 (MQ=255)
                            cccACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCg                                         >  1:7332323/1‑76 (MQ=255)
                            cccACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCg                                         >  1:5846893/1‑76 (MQ=255)
                            cccACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCg                                         >  1:2251395/1‑76 (MQ=255)
                            cccACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCg                                         >  1:10044761/1‑76 (MQ=255)
                             ccACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCgg                                        >  2:1031162/1‑76 (MQ=255)
                             ccACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCgg                                        >  2:2181936/1‑76 (MQ=255)
                              cACTGACCAAAAGACGCCCCAGCGCCTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGt                                       <  2:8945357/76‑1 (MQ=255)
                              cACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGt                                       <  1:489518/76‑1 (MQ=255)
                              cACGGACCAAAAGACGCCCCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGt                                       <  1:769468/76‑1 (MQ=255)
                               aCTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCggca                                      <  1:9771791/76‑3 (MQ=255)
                               aCTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTa                                      <  1:4632178/76‑1 (MQ=255)
                                cTGCCCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTAc                                     <  1:6105037/76‑1 (MQ=255)
                                cTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTAc                                     >  1:1136954/1‑76 (MQ=255)
                                  gACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCg                                   >  1:4654888/1‑76 (MQ=255)
                                  gACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCg                                   >  1:7791695/1‑76 (MQ=255)
                                  gACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCg                                   >  2:2049273/1‑76 (MQ=255)
                                   aCCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGa                                  >  1:3485621/1‑76 (MQ=255)
                                    ccAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAg                                 <  1:6546015/76‑1 (MQ=255)
                                    ccAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAg                                 >  2:1476378/1‑76 (MQ=255)
                                      aaaaGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGa                               >  2:10035152/1‑76 (MQ=255)
                                      aaaaGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGa                               <  1:9818390/76‑1 (MQ=255)
                                         aGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAAtt                            <  1:6702694/76‑1 (MQ=255)
                                             gCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGc                        >  2:7199304/1‑76 (MQ=255)
                                             gCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGc                        >  2:6294609/1‑76 (MQ=255)
                                             gCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGc                        >  2:397993/1‑76 (MQ=255)
                                             gCACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGc                        >  2:1150737/1‑76 (MQ=255)
                                             gCACCAGCGACTTTTAGTGCTGCAACGCA‑‑A‑‑CCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCCGCaa                      >  2:3016304/1‑76 (MQ=255)
                                              cACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCa                       <  1:5550387/76‑1 (MQ=255)
                                              cACCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCa                       >  2:5846920/1‑76 (MQ=255)
                                               aCCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCaa                      >  1:9272782/1‑76 (MQ=255)
                                               aCCAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCaa                      >  1:2728970/1‑76 (MQ=255)
                                                ccAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAAt                     <  2:6838869/76‑1 (MQ=255)
                                                 cAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATg                    >  1:5653061/1‑76 (MQ=255)
                                                 cAGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATg                    >  1:1936097/1‑76 (MQ=255)
                                                  aGCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGc                   >  2:5572735/1‑76 (MQ=255)
                                                   gCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGCa                  >  1:9633435/1‑76 (MQ=255)
                                                   gCGACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGCa                  <  1:4193727/76‑1 (MQ=255)
                                                     gACTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGCACt                <  1:4028272/76‑1 (MQ=255)
                                                       cTTTTAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGCACTgg              >  1:3786749/1‑76 (MQ=255)
                                                        ttttAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGCACTGGc             >  2:7077038/1‑76 (MQ=255)
                                                        ttttAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGCACTGGc             >  2:2631872/1‑76 (MQ=255)
                                                         tttAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGCACTGGcc            >  2:9187565/1‑76 (MQ=255)
                                                         tttAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGCACTGGcc            >  2:7932800/1‑76 (MQ=255)
                                                          ttAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGCACTGGCCt           >  1:8757223/1‑76 (MQ=255)
                                                          ttAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGCACTGGCCt           >  1:830683/1‑76 (MQ=255)
                                                           tAGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGCACTGGCCtt          >  2:7327328/1‑76 (MQ=255)
                                                            aGTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGCACTGGCCttt         <  1:8590455/76‑1 (MQ=255)
                                                             gTGCTGCAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGCACTGGCCTTTc        >  2:8119541/1‑76 (MQ=255)
                                                              tgctgcAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGCACTGGCCTTTCa       <  1:2383608/76‑1 (MQ=255)
                                                              tgctgcAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGCACTGGCCTTTCa       <  2:9388628/76‑1 (MQ=255)
                                                              tgctgcAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGCACTGGCCTTTCa       <  1:8414894/76‑1 (MQ=255)
                                                              tgctgcAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGCACTGGCCTTTCa       <  2:5277535/76‑1 (MQ=255)
                                                                 tgcAACGCA‑‑ACCCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGCACTGGCCTTTCAAgg    >  2:4842089/1‑76 (MQ=255)
                                                                  gcaacgca‑‑accCCCACACCGAAGAAAATCAACACCCCGGTACCGAGGAATTCAGCAAGGCACTGGCCTTTCAAGGt   <  2:7579400/76‑1 (MQ=255)
                                                                   caacgca‑‑accCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGCACTGGCCTTTCAAGGtt  <  2:3636418/76‑1 (MQ=255)
                                                                   caacgca‑‑accCCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGCACTGGCCTTTCAAGGtt  <  1:3313005/76‑1 (MQ=255)
                                                                             ||                                                                  
ACCCCCAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCA‑‑A‑‑CCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATTCAGCAATGCACTGGCCTTTCAAGGTT  >  REL606/4097418‑4097558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑