| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,376,348 | 0 | T | G | 44.5% | 248.3 / 65.2 | 191 | T63P (ACC→CCC) | folC | bifunctional folylpolyglutamate synthase/ dihydrofolate synthase |
| Reads supporting (aligned to +/- strand): ref base T (19/87); new base G (85/0); total (104/87) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.81e-36 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 261 total aligned reads displayed.
GCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAC > REL606/2376273‑2376423 | gCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑ggt < 1:5443837/76‑1 (MQ=255) cACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑ggtg > 2:3940934/1‑76 (MQ=255) cACCAGATGAGGCGAACTGTAGACCCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑ggtg > 2:2438383/1‑76 (MQ=255) ccAGATGAGGCGAACTGTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑ggtggt < 1:3513501/76‑1 (MQ=255) aGATGAGGCAAACTCAAGACCCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGc < 2:9456047/76‑1 (MQ=255) tGAGGCGACCTTTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCtt < 2:10013231/76‑1 (MQ=255) gAGGCGAACTGTAGACGCCCTCTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCttt < 2:9889382/76‑1 (MQ=255) gAGGCGAACTGTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCttt < 2:7572586/76‑1 (MQ=255) gAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCttt < 1:5099519/76‑1 (MQ=255) gAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCttt < 2:3672641/76‑1 (MQ=255) aaGGCGAACGGAAGACGCCCCCTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCttt < 2:1841718/75‑1 (MQ=255) aGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCCTTg > 2:427516/1‑76 (MQ=255) cGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCGTTGCCa > 1:3673417/1‑76 (MQ=255) gAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCAt > 1:9144462/1‑76 (MQ=255) aaCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCAtt > 1:2193092/1‑76 (MQ=255) aaCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGCgcccttgccctt > 1:6680203/1‑76 (MQ=255) aaCTGAAGACACCCACTTTGTACCCGGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCAtt < 2:8140305/76‑1 (MQ=255) cTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTGCCTTTTCCAtttg > 1:4651903/1‑74 (MQ=255) tGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCAATTCGt > 1:4830270/1‑76 (MQ=255) tGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTGGCCTTGCCATTcct > 1:1213286/1‑74 (MQ=255) gttAGACGCCCCCTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGt < 1:5787303/74‑1 (MQ=255) gTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCCTTGGCATTCGTa > 1:10265643/1‑76 (MQ=255) gTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCCTTGCCATTCgga > 2:2662351/1‑74 (MQ=255) tAGCCGCCCCCTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTAc < 2:9069272/76‑1 (MQ=255) tAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTGCCTTTGCCATTCGTAc > 1:7752164/1‑76 (MQ=255) tAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGTAc > 1:1425766/1‑76 (MQ=255) tAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGGAc > 1:6982124/1‑76 (MQ=255) agccGCCCACTTTCTCCCCTCCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTAcc < 2:905399/73‑1 (MQ=255) aGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGTAcc > 1:9775368/1‑76 (MQ=255) ccccccATTTTGTACCCTCCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCg < 2:7292522/73‑1 (MQ=255) acccccATTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCg < 1:6108819/73‑1 (MQ=255) cGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTGCCTTTGCCATTCGTACCCGc > 2:2106817/1‑76 (MQ=255) ccACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGGCTTTGCCATTCGTACCCGCCAc > 1:2558039/1‑76 (MQ=255) cccTTTTTCCCTTGCCGCCAGCAGAATCTCCCCCAGCCTACGTGA‑GTTGGTGCCTTTGCCATTCGTTCCCGCAACg < 2:10269931/74‑1 (MQ=255) cACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGTGCCTTTGCCCTTCGTCCCCGCAACg > 1:2644125/1‑76 (MQ=255) aCTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGGCATTCGTACCCCCAACgg > 2:7484061/1‑76 (MQ=255) cTTTGTACCCTGCCNCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGTAACCGCAACCGt > 1:3161934/1‑76 (MQ=255) cTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGGCATTCGGACCCCCAACGGt > 1:6491671/1‑76 (MQ=255) cTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGTACCCCCCACggg > 2:8889278/1‑75 (MQ=255) cTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCCTTGCCATTCGTACCCGCAACggg > 1:3981078/1‑75 (MQ=255) tttGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTa < 1:1178585/76‑1 (MQ=255) ttGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGGGCCCTTGTCATTCGTACCCCCCACGGGaa > 1:843106/1‑76 (MQ=255) tGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTaaa < 2:1969986/76‑1 (MQ=255) tGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGCCTTTGCC‑TTCGTACCCGCAACGGCaaa > 1:9560487/1‑76 (MQ=255) tGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGGGCCTTTGCCATTCGTACCCCCAACGGCaaa > 2:4524699/1‑76 (MQ=255) tGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGTACCCGCACCGGTaaa > 2:4461566/1‑76 (MQ=255) gTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGGCATTCGTACCCGCAACGGTAAac > 1:4587916/1‑76 (MQ=255) gTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAac > 1:1888377/1‑76 (MQ=255) tACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGTACCCGCCACGGTAAaca > 2:579416/1‑76 (MQ=255) cccccTGCCCCCTTCAGAATCGCCTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTCCCCGCAACGGTAAaca < 1:6632397/74‑1 (MQ=255) ccccTCCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAacac < 2:7930916/75‑1 (MQ=255) aCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGTACCCGCAACGGGAAacaa > 2:2840238/1‑75 (MQ=255) aCCCTGCCGCCATCACAATCGACTCCAGAGTACGGCA‑CGTGGGGCCTTTGACATTCGTGACAGCCACGTTAAacac > 2:9196769/1‑76 (MQ=255) cccTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGGCATTCGTACCCGCAACGGCAAAcaca > 2:113695/1‑76 (MQ=255) ccTCCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACaa < 2:3707831/76‑1 (MQ=255) cggccgccATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACaaa < 1:2173295/74‑1 (MQ=255) cTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAAAACaaa > 1:5273274/1‑76 (MQ=255) tGCCGCCATCAGAATCGCCTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAt < 2:5589133/76‑1 (MQ=255) tGCCGCCATCAGAATCGACCCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAt < 1:9056399/76‑1 (MQ=255) ggccgccATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAt < 2:5013965/75‑1 (MQ=255) gccgccATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATg < 1:7325617/76‑1 (MQ=255) ccgccATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGGCATTCTTACCCGCCAAGGTAAACAAAAATgg > 1:7136857/1‑76 (MQ=255) ccgccATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTTGGACCCGCAACGGGGAACACAAAAgg > 2:9735002/1‑76 (MQ=255) cccccAGCAGAACCGCCTCCAGCGTACGGCA‑GGTGGGACCTTTGCCATTCGTACCCGCAACGGTAAACACAAATgg < 1:2001934/73‑1 (MQ=255) ccATCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAACAAAAATGgcgc > 2:1607601/1‑76 (MQ=255) aTCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGTACCCGCAACGGGAAACACAAATGGGGCTg > 1:8738845/1‑76 (MQ=255) aTCAGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCCTTGCCATTCGTACCCGCCACGGTAAACACAAAAGGCGCTg > 1:1552770/1‑76 (MQ=255) tCAGAATCGACTCCAGCGTACGGCA‑GGGGGTGCCCTTGACATTCGTACCCGCCACGGTAAACACAAAAGGCGCTgg > 1:5483519/1‑76 (MQ=255) cAGAATCGACTCCAGCGTACGGCA‑GGGGGTGCCTTTGCCCTTCGTACCCCCAACGGGAAACACAAATGGCGCTggg > 1:3791601/1‑75 (MQ=255) cAGAATCGACTCCAGCGTACGGCA‑GGGGGTGCCTTTGCCACTCGTAACCGCCACGGCAAACACAAAAAGGGCTGGt > 1:2331063/1‑76 (MQ=255) aGAATCGACTCCGGCGTACGGCA‑GGGGGGGGCCTTGGCATTCGGACCCGCAACGGGAAACACAAAAGGCGCTgggt > 2:5424516/1‑74 (MQ=255) aGAATCGACTCCAGCGTACGGCA‑GGGGGGGCCCTTGCCATTCGTACCCGCAACGGGAAACACAAATGGCGCTGGtt > 2:4867318/1‑76 (MQ=255) aaTCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTc < 2:6392389/76‑1 (MQ=255) aaTCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAAAACAAATGGCGCTGGTTTc > 1:4370528/1‑76 (MQ=255) aTCGACTCCAGCGTACGGCA‑GGGGGTGCCTTTGCCATTCGTACCCGCCACGGGAAAAACCAATGGCGCTGGttttc > 1:16855/1‑74 (MQ=255) tCGACTCCAGCGTACGGCA‑GGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGGTTCAg > 2:9269938/1‑76 (MQ=255) gcaaCTCCAGCGGACGGCG‑GGTGGAGCCTTTACCATTCGTAACCGCATCGGTAAACACAAATGGCGCTGGTTTCAg < 1:2857776/73‑1 (MQ=255) cGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAgg < 1:8133868/76‑1 (MQ=255) gACTCCAGCGTACGGCA‑GGGGGGGCATCTGCCGTTCGTACCCGCCGAGGTAAACACAAATGTCGCTGGTTTCCGGa > 2:695279/1‑76 (MQ=255) cTCCAGCGTACGGCA‑GGTGGTGCCTTTGACATTCGCAACCGCTAAGGGAAACACAAAGGGAGCTGGGTTTacgacg > 2:6279172/1‑76 (MQ=255) cgaGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGcc < 2:5469761/74‑1 (MQ=255) cAGCGTACGGCA‑GTTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCa < 1:6861252/76‑1 (MQ=255) cAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCa < 1:10012498/76‑1 (MQ=255) aGCGTACTGCG‑GGTGGTCCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCaa < 1:4502964/76‑1 (MQ=255) aGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCaa < 1:6812485/76‑1 (MQ=255) aGCGTACGGCA‑GGGGGTGCCTTTGCCATTCCTACCCCCAAAGGGAAACACAAATGGCGCTGGTTTCAGGAGGccca > 2:9638498/1‑74 (MQ=255) gCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAg < 2:3080982/76‑1 (MQ=255) gCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAg < 1:8590882/76‑1 (MQ=255) gTACGGCA‑GGGGGTGCCTTTGCCATTCGTACCCGCCACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAc > 2:6464423/1‑76 (MQ=255) tACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAcg < 2:10163197/76‑1 (MQ=255) tACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAcg < 2:5341408/76‑1 (MQ=255) gCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgcc > 2:6640948/1‑76 (MQ=255) cA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccg > 2:4241635/1‑76 (MQ=255) cgggtggtGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccg < 2:5756087/74‑1 (MQ=255) a‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccgc > 2:2240616/1‑76 (MQ=255) a‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccgc > 1:784822/1‑76 (MQ=255) ggtggtGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCcgcg > 2:3381012/1‑76 (MQ=255) ggtggtCCCTTTGCCTTTCGTCCCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCcgcg < 1:5842317/76‑1 (MQ=255) gtggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGa > 1:5532010/1‑76 (MQ=255) tggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAc > 1:315496/1‑76 (MQ=255) | GCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCA‑GGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAC > REL606/2376273‑2376423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |