| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,515,909 | 0 | A | C | 58.5% | 45.8 / 109.0 | 82 | V260G (GTG→GGG) | ypfI | predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (21/13); new base C (0/48); total (21/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.06e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.48e-14 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 139 total aligned reads displayed.
GTTAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATT > REL606/2515834‑2515984 | gTTAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTCATCGacca < 1:7631781/76‑1 (MQ=255)gTTAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTCATCGacca < 1:6969100/76‑1 (MQ=255) ttAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGGTTCATCTaccac > 1:4223478/1‑76 (MQ=255) ttAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTCATCGaccac < 1:9867164/76‑1 (MQ=255) ttAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTCATCGaccac < 2:2167364/76‑1 (MQ=255) ttAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTCATCGaccac < 1:9585181/76‑1 (MQ=255) ttAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTCATCGaccac < 2:8826710/76‑1 (MQ=255) tAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTCATCGaccacc < 2:6432794/76‑1 (MQ=255) tAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTCATCGaccacc < 1:304748/76‑1 (MQ=255) aacaacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTCATCGAccacca < 1:9669075/76‑1 (MQ=255) aacaacGTTCGAGAAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTCATCGAccacca < 2:2548159/76‑1 (MQ=255) acaacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTCATCGACCAccag < 2:4835173/76‑1 (MQ=255) acaacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTCATCGACCAccag < 2:144945/76‑1 (MQ=255) acaacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTCATCGACCAccag < 1:1706083/76‑1 (MQ=255) caacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGGGGGTTTTTTGAcccccacc > 2:3967914/1‑74 (MQ=255) caacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTTCTCCaccacccac > 2:10085935/1‑73 (MQ=255) caacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTCATCGACCAccagc < 1:1924563/76‑1 (MQ=255) aacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTCATCGACCAccagcc < 1:3318799/76‑1 (MQ=255) aacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTCATCGACCAccagcc < 1:4954154/76‑1 (MQ=255) aacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTCATCGACCAccagcc < 1:4949825/76‑1 (MQ=255) aacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTCATCGACCAccagcc < 1:4526375/76‑1 (MQ=255) ttCGAGGAAAACGCGATACCAGTTGATGCAAAAAGGGCGCAGGTA‑TGGCTGCGGCTTCATCGCCCCCCAGCCAGTc < 2:6381802/76‑1 (MQ=255) ttCGAGAAAAACCGAAGACAAGTTGATGAAACAATGGCGCAGGTA‑GGGCTGCGGCTTCATCGCCCCCCAGCCAGTc < 2:7928913/76‑1 (MQ=255) gcGAGGAAAACGCGATACCAGTTGATCCAACAAGGGCGAAGGTA‑TGCCGGCGCCTTCATCCCCCCCCAGCCAGTCg < 2:6921768/75‑1 (MQ=255) ggAAAACGCGATCCCAGTTGATCCAACAAGGGCGCAGGTA‑GGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCtt < 2:8400903/76‑1 (MQ=255) gaaaaaCGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCGGCGGCTTCATCGCCCCCCAGCCAGTCGGCtt < 2:620327/74‑1 (MQ=255) aaaacCGCGATCCCAGTTGATGCAACAAGGGCGCGGGTA‑TGGCTGCGTCTTCATCGCCCCCCAGCCAGTCGGCTTg < 2:9167714/75‑1 (MQ=255) aaaaCGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGGGGCTTCATTCGCCACCAGCCACCCGGCTTgt > 1:6776344/1‑75 (MQ=255) aaaaCGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTTATCGATCCCCAACCAATCGGGtttc > 1:1614222/1‑74 (MQ=255) aaCGCGATACCAGTTGATGCAACAATGGCGCGGGTA‑TGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTc < 1:1949884/76‑1 (MQ=255) aaCGCGACACAGGTTAATACAACACGGGCGCTGGTA‑TGTCTCCGGCTTCATCGACCCCCAGCCAGTCGGCTTGCTc < 1:6916860/76‑1 (MQ=255) aCGCGATACCAGTTGATGAAAAAAGGGCGCGGTTA‑TGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCa < 2:8323556/76‑1 (MQ=255) cgcgACCCCGGTTGGTGCAAAAATGGCGCAGGTA‑TGGCTGCGGCTTCAGCGCCCCCCAGCCAGTCGGCTTGCTCAt < 1:8322302/76‑1 (MQ=255) ccgACACCATTTGAAGTAAAAAGGGCGGATGTA‑GGCCCGCGGCTTCATCGACCCCCAGCCAGTCGGCTTGCTCATc < 2:6283174/75‑1 (MQ=255) atcccAGTTGATGCAAAAATGGCGCGGGTA‑TGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCt < 1:6272394/73‑1 (MQ=255) cggTTGACGCAACAAGGGCGCGGGTA‑GGCCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCt < 1:9182527/74‑1 (MQ=255) cAGTTGATCCACAAAGGGCGCAGTTA‑TGGCGGGGCCTTAATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCt < 2:3573307/76‑1 (MQ=255) cAGTGGATGCACCAAGGGCGCGGGTA‑TGCCTGCGGCTTCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCt < 2:6107527/76‑1 (MQ=255) cAGTGGATGCAACAAGGGCGCAGGTA‑TGGCTGCGGCTTCATCGCCCACCAGCCAGTCGGCTTGCTCATCGCTGGCt < 1:4482159/76‑1 (MQ=255) aGTTGATGCAACAAGGGCGCGGGTG‑TGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑a < 1:8606431/76‑1 (MQ=255) tttaTGCAACAATGGCGCGGGTC‑TGCGTGCGGCTTCATCCCCCCCCAGCCAGTCGGCTTGCTCATCGCTAGCT‑AAc < 1:2461575/73‑1 (MQ=255) ttGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTTATCGA‑AACCAGCCAGACAGGGTTCTCCTCGCTCGCT‑aaaa > 1:9865608/1‑74 (MQ=255) ttGATGCAAAAATGGCGCAGGTT‑GGGCTGCGGCTTCTGCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AAc < 1:5099559/76‑1 (MQ=255) tagATGCAAGAAGGACGCAGTCC‑TGGCTCGGCCTTCACCACCCCCCAGCCAGTCGGCTTTCTCATCGCTGGCT‑AAc < 1:2410996/74‑1 (MQ=255) gtGAGGCAAAAAGGGCCCGGGTA‑TGGCTGCGTCTTCATCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AAc < 1:1837362/75‑1 (MQ=255) tgatgaAAGAAGGGCGCAGTTC‑TGGCGGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACa < 1:6995645/76‑1 (MQ=255) gATGCAACAATGGCGCGGGTA‑TGGCTGCGGCTTCATCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACaa < 1:10121520/76‑1 (MQ=255) gATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACaa < 1:2124316/76‑1 (MQ=255) gATGAAACAGGGGGGCAGGTA‑GGCCGGCGGTTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACaa < 2:246218/76‑1 (MQ=255) tGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTCATCGACCCCCAGCCCGGCGGGTTTCTCCTCGCCGGCT‑AAAAAgg > 1:4311069/1‑76 (MQ=255) acaaacagTGGCGCAGGTA‑TGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAgg < 2:384056/73‑1 (MQ=255) gaaacagTGGCGAGGGTC‑TGGCTGCGCTTGCATCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGc < 2:19626/74‑1 (MQ=255) caacaaTGGCGCAGGTA‑TGGCTGCGGCTTCATCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCa < 1:7693632/76‑1 (MQ=255) aacaaGGGCGCGGGTA‑TGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCAt < 1:9358304/76‑1 (MQ=255) acaaGGGCG‑AGGTATTGGCTGCGGCTCCACCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATc < 2:6336376/76‑1 (MQ=255) aaaaTGGCGCGGGTG‑TGGCTGCGGCTTCACCGCCCACCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATc < 2:450985/74‑1 (MQ=255) tGGCGCAGGTA‑TGGCTGCGGCTTCATCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGgc < 1:5150292/76‑1 (MQ=255) cgcgGGTG‑TGGCTGGGGCTTCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCa < 1:3484558/76‑1 (MQ=255) gcAGGTA‑TGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTTCTCATCGCTGGCTAAACAAGGCACCGGGGGCa > 1:8695392/1‑76 (MQ=255) gTA‑TGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCCCATCGCTGGCT‑AACAAGGCATTCCGGGCCATaaa > 1:2776202/1‑76 (MQ=255) ttgggcggcGCCTTCTCCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAg < 1:6079818/73‑1 (MQ=255) a‑TGGCTGCGGCTTTATTGACCACCAGCCAATCCGGTTTTGCCTCGCTTGCTAAAAAAGGCTTCCGGGCCaaaaaac > 2:4753312/1‑75 (MQ=255) ggggcggcggcTTCATCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGc < 2:4926791/74‑1 (MQ=255) tGGCTGCGGCTTTATCGACCACCAGCCAGTCGGCTTGCTCATCGCCGGGC‑AAAAAAGGATTCGGCGCCATAAAACg > 2:5355639/1‑76 (MQ=255) tGGCTGCGGCTTCATCGCCCACCAGCCAGTCGGCTTGCTCCTCGCTCGCTAAACAAGG‑ATACAGCGCCATaaaagg > 2:9151231/1‑73 (MQ=255) tGGCTGCGGCTTCATCGACCACCAACCAGGCGGGTTTCTCATCTCTCGCC‑AACAAAGCAACCGGCGCCACAAAACg > 2:3217467/1‑76 (MQ=255) gCTGCGGCTTCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGaa < 1:2211240/76‑1 (MQ=255) cTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGGATCCGGCGCAATAAAGCGaaa > 1:6301591/1‑76 (MQ=255) cTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGGAACCGGCGCAATAAAGCGgaa > 1:2218836/1‑76 (MQ=255) cTGCGCCTTCATCCCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGaaa < 1:4316331/76‑1 (MQ=255) cTGCGACTTCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGaaa < 1:2870046/76‑1 (MQ=255) tGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGGT‑AACAAAGCATCCCGCGCCAAAAAACGaaaa > 2:8916975/1‑75 (MQ=255) gCGGCTTCATCGACCACCAGCCAGTCGGCTTTGTCATCGCTGGGC‑AAAAAGCCAGCCGGCGCGATAAAACGcaaac > 2:8364044/1‑74 (MQ=255) gCGGCTTCATCGACCACCAGCCAGTCGGCTTTCTCCTCGCTTGCC‑AAAAAAGGATCCCGCGCAATAAAACGaaaat > 2:4498180/1‑74 (MQ=255) gCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACt < 2:7628245/76‑1 (MQ=255) cGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACtt < 1:1195782/76‑1 (MQ=255) ggCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTtc > 1:7710422/1‑76 (MQ=255) ggCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTtc > 1:9224374/1‑76 (MQ=255) gCTCCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTtct < 1:7202759/76‑1 (MQ=255) cTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTtctc < 1:4569709/76‑1 (MQ=255) cTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTtctc < 1:8679503/76‑1 (MQ=255) cTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTtctc < 2:5644792/76‑1 (MQ=255) cTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTtctc > 1:9558194/1‑76 (MQ=255) ttCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGGATCCGGCGCAATAAAGCGAAACTTCTCg > 2:3374254/1‑76 (MQ=255) ttCATCCCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCg < 1:5026935/76‑1 (MQ=255) tCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGc < 2:2876241/76‑1 (MQ=255) tCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCCTCCGGCGCAATAAAGCGAAACTTCTCGc > 2:7055287/1‑76 (MQ=255) tCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGc > 1:10357878/1‑76 (MQ=255) tCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGc > 1:9732229/1‑76 (MQ=255) tCATCCCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGc < 2:2493245/76‑1 (MQ=255) cATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGcc > 2:8614513/1‑76 (MQ=255) aTCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AAAAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGccc > 2:3628989/1‑76 (MQ=255) gcGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCg < 2:4974528/75‑1 (MQ=255) ccGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCg < 2:4492315/75‑1 (MQ=255) ccacccccAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGc < 2:1976642/74‑1 (MQ=255) accaccAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCaa > 2:5149870/1‑76 (MQ=255) caccaGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCCAAt > 1:5388810/1‑76 (MQ=255) caccaGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAAt > 1:6185529/1‑76 (MQ=255) accaGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAAtt > 2:1707363/1‑76 (MQ=255) accaGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAAtt > 1:7870971/1‑76 (MQ=255) | GTTAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTA‑TGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCT‑AACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATT > REL606/2515834‑2515984 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |