| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,180,249 | 0 | T | G | 46.1% | 195.4 / 65.2 | 154 | G24G (GGT→GGG) | yjaH | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (20/63); new base G (71/0); total (91/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.30e-26 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 303 total aligned reads displayed.
GGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCAG > REL606/4180174‑4180324 | ggCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGt < 2:1206246/76‑1 (MQ=255) gCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTg < 1:6615980/76‑1 (MQ=255) gCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTg < 2:4712796/76‑1 (MQ=255) cAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTgc < 2:10166038/76‑1 (MQ=255) aGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTgcg < 2:6832514/76‑1 (MQ=255) aGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGgcg > 2:9680204/1‑76 (MQ=255) aaCAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGt < 2:5576898/76‑1 (MQ=255) aGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTtc < 1:4704082/76‑1 (MQ=255) ttttAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTtctc < 2:1492093/76‑1 (MQ=255) tttAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGGTGTTtctta > 1:8294462/1‑74 (MQ=255) tttAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTtttcc > 1:2549489/1‑75 (MQ=255) ttAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCAc < 1:5733551/76‑1 (MQ=255) ttAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCCGTTTTTCAc > 1:4382970/1‑76 (MQ=255) tAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACt < 1:4051242/76‑1 (MQ=255) tAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACt < 1:4669857/76‑1 (MQ=255) tAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACt < 1:4637491/76‑1 (MQ=255) aaCGAAGGGGTTGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTg < 2:7750650/76‑1 (MQ=255) aCGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGc < 2:420169/76‑1 (MQ=255) aCGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTCTCTCTGc > 2:6995309/1‑76 (MQ=255) cGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCACTGcc > 2:9818106/1‑76 (MQ=255) gAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTCTCCCTGCCt > 2:10210914/1‑76 (MQ=255) gAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCGGGTTTTCACTGCCt > 1:1314723/1‑76 (MQ=255) gAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCCGGTTTTCACTTccc > 1:6246645/1‑75 (MQ=255) gAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCCGTTTCTCTCTGCCt > 2:9071260/1‑76 (MQ=255) gAAGGCGTGCTTTCTCCCCTTTTGTGTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCt < 1:198076/76‑1 (MQ=255) aaGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCCCTTccct > 2:6166094/1‑74 (MQ=255) aaGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGGTTTTTcctgcctg > 2:8311281/1‑76 (MQ=255) aaGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGCGCGCTGTTTCTCTCTGCCTg > 2:7396433/1‑76 (MQ=255) aaGGGGTGGTTTAACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCtt > 2:4321525/1‑75 (MQ=255) aGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGt < 1:9439464/76‑1 (MQ=255) aGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCCGTTTCTccctccctct > 2:3555232/1‑74 (MQ=255) ggggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGGCCTGGTTCTCGCTTCCTGTa > 2:9587107/1‑76 (MQ=255) ggggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTTTCCCTGCCTGTa > 2:2283915/1‑76 (MQ=255) ggggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTa < 1:5297782/76‑1 (MQ=255) ggggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTa < 1:2170891/76‑1 (MQ=255) ggggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGGTTCTTCCTGCCCGtt > 1:1016971/1‑75 (MQ=255) gggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAg < 1:9870689/76‑1 (MQ=255) gggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGGTTTTCACTGCCTTTAg > 2:9388443/1‑76 (MQ=255) ggtggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGc < 2:10043932/76‑1 (MQ=255) tggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCca < 1:8957955/76‑1 (MQ=255) ggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCcac > 1:9318640/1‑76 (MQ=255) tttCCCCCTTTTTGTCTTTCGGGCGTCGATCATTGATGCTGGCGGGTGCTCTTTTTCTCCCTGCCTTTAGCCACaa < 2:2883925/76‑1 (MQ=255) tCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGAGGCTTTTCACTGCCTGTAAACACAAca > 2:8057813/1‑75 (MQ=255) tCACCCCTTTTGTCTTTCTGACGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACCGCCTGCTGCCACaaat > 2:6606395/1‑74 (MQ=255) cgccccTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCGGTTTTTCACTCGCCGTAGGCACAActc > 1:4248890/3‑76 (MQ=255) cACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTTTCACTGCCTGTAGGCACCActc > 2:6040678/1‑76 (MQ=255) ccccTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTTTCACTGCCTGTAGCCACAACTCtt > 1:161630/1‑76 (MQ=255) cccTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTCTCCCTGCCCGTCGCCACAACTCTTc > 1:4207467/1‑76 (MQ=255) ccTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTTTCACTGCCTGTAGCCACAAATCTTCa > 2:1650843/1‑76 (MQ=255) ccTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGGTGTTTTTCCCTGCCTGGAGCCACAACTTTTcc > 1:6835451/1‑75 (MQ=255) cTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCACTGCCCGTTGCCACCAATCtttcc > 1:5851439/1‑73 (MQ=255) ttttGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTTTCCCTGCCCGCCGCCACCACCCTTTACt > 1:365133/1‑76 (MQ=255) ttttGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCGGTTTTTCTCGTCCTATAAGCACAACTCTTCCCt > 1:3942424/1‑76 (MQ=255) tttGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCCGTTTCTCACTGCCTGTAGCCACCACTCTTCACtt > 1:4614340/1‑76 (MQ=255) ttGTCTTTCTGGCGTCGATCATTGATGCTGGCGGGTGCGCTGTTTCTCCCTGCCTGTAGCCACAACTCTTCACTtc < 2:58868/76‑1 (MQ=255) tGTCTTTCTGGGGTCGATCATTGATGCTGGCAGGGGCGCTTTTTTTCCCCGCCCTTAGCCACAACTCTTCACCtcc > 2:6242203/1‑76 (MQ=255) tGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCCCTGCCTTTAGCCCCAACACTTCACTtcc > 1:8431477/1‑76 (MQ=255) tGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTtcc > 1:5969575/1‑76 (MQ=255) tGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCCGGTTTTCTCTGCCTGTAGCCACAAATCTCCACtttc > 1:970149/1‑74 (MQ=255) gTCTTTCGGGCGGCGATCATTGATGCGGGCGGGTGCGCTGTTTCCCCCTGCCTTTAGCCACAACTCTTCACTtcct < 2:1928224/76‑1 (MQ=255) tcgttCTGGCTGCGGTCTTAGATGCTGCCAGGTGGGCTGTTTCTCACCGCTTTTAGCCACAACTCTTCACTtcctc < 2:2468725/73‑1 (MQ=255) tCTTTCTGGCGTCTATCATTGATGCTGGCGGGTGCGCTTTTTCTCCCTGCCTTTAGCCACAACTCTTCACTtcctc < 2:5256549/76‑1 (MQ=255) tCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCCCTGCCTGTAGCCACAACTCTTCACTtcctc > 1:9622325/1‑76 (MQ=255) tCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCACTGCCTGTAGCCACAACTCTTTACTtcctc > 1:7245200/1‑76 (MQ=255) cTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTttctcc > 2:8676577/1‑76 (MQ=255) tttCTGGCGTCGATCTTTGATGGTGGGGGGTGCCCTTTTTCTCCCTGCTTTTAGCCACAACTCTTCACTTCCTccc < 2:1387454/76‑1 (MQ=255) tttCTGGCGTCGATCATTGATGCTGGCAGGTGCGCGGTTTCTCACTGCCTGTTGCCACCACTCTTCACTTcccccc > 1:4849654/1‑76 (MQ=255) tttCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTTTCCCCGCCTGTAGCCCCAACTCTTCACTTCCTccc > 1:6440044/1‑76 (MQ=255) tttCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCACTGCCTGTAGCCACAACTCTTCACTTCCTccc > 2:3249321/1‑76 (MQ=255) tttCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCCGTAGCCCAAAATCTTCACTTCCTccc > 1:7343960/1‑76 (MQ=255) ttCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCt > 1:2730903/1‑76 (MQ=255) ttCTGGCGTCGATCATTGATGCTGGCAGGGGCGCCGTTTCTCACACCCCGTAGCCACAAATCTTCACTTTCTcccc > 1:8719624/1‑75 (MQ=255) tCTGGCGTCGATCATTGATGCTGGCAGGTGCCCTGTTTCTCCCTGCCTGTAGCCACAACTCTTCACTTCCTCCCtt < 1:239749/76‑1 (MQ=255) cTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTTTCACTGCCTGTAGCCACAACTCTTCACCTCCTCCCctt > 2:4509520/1‑76 (MQ=255) tGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTa > 2:3774960/1‑76 (MQ=255) ggggTCGATCATGGATGCTGGCGGGTGCGCTTTTTCTCCCTGCCTTTAGCCACAACTCTTCACTTCCTCCCTTTAc < 2:4072658/73‑1 (MQ=255) ggCGTCGGTCTTTGGTGTGGGCAGGTGCGTTTTTTCTCACTGCCTTTAGCCACAACTCTTCACTTCCTCCCTTTAc < 1:3451322/76‑1 (MQ=255) ggCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCCCTTCCTTTAGCCACAAATCTTCACTTCCTCTCttttt > 2:1547618/1‑74 (MQ=255) ggCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCAATGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTAc > 2:5106525/1‑76 (MQ=255) gCGTCGATCTTTGATGCTGGCGGGGGCGCTGTTTCTCACTCCCTGTAGCCACAACTCTTCACTTCCTCCCTTTAcc < 2:2548552/76‑1 (MQ=255) tCGATCTTGGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGca < 1:3106260/76‑2 (MQ=255) tCGATCATTGATGCTGGCGGGTGCGCTTTTTCTCACTGCCTTTAGCCACAACTCTTCACTTCCTCCCTTTACCGcc < 1:6071180/76‑1 (MQ=255) cGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCa > 1:7726003/1‑76 (MQ=255) gATTATTGATGGTGGCAGTGGCCTTTTTTCTCCCTGCCTTTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAg < 1:2908736/76‑1 (MQ=255) gATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTATCCCCAg > 2:6410092/1‑76 (MQ=255) tCATTGATGCTGGCAGGGGCGCTGTTTTTCACTGCCTGTAGCCACAACTCTTCACTTTCTCCCTTTTCCGCCAGTg > 2:5359274/1‑76 (MQ=255) tCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCCGTg > 1:1045659/1‑76 (MQ=255) ttgttgCTGGCGGGTGCCTTTTTCCCCCCTGCCTTTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGAt < 1:7056598/76‑1 (MQ=255) tgttGCGGGCAGGGGCGCGGTTTCTCCCTCCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGAtt < 1:5146649/73‑1 (MQ=255) tGATGCTGGGAGGTGCGTTGTTTCTCCCTGCTTTTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGAtt < 1:765928/76‑1 (MQ=255) tGATGCTGGCGGGGGCCCTTTTTCCCCCTGCCTTTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGAtt < 1:5624165/76‑1 (MQ=255) tGATGCGGGCGGTTGCTCTTTTTCTCCCTGCCTTTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGAtt < 1:6865792/76‑1 (MQ=255) gATGCTGGCAGGTGCGCTGTTTCTCCCCGCCTGGAGCCACAACCCTTCTCTTCCTCCCTTTACCGCCAGTGGAttt > 2:890418/1‑76 (MQ=255) gATGCTGGCAGGGGCGCTGTTTTTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCCGTGGAttt > 2:10325561/1‑76 (MQ=255) gtGGCGGGTGCGTTTTTCCCCCCTCCTTTTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTg < 2:5667688/75‑1 (MQ=255) tGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGa > 1:7575341/1‑76 (MQ=255) ggTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGAcc > 2:8593541/1‑76 (MQ=255) ggTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGAcc > 2:3026560/1‑76 (MQ=255) gTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCa > 1:1870369/1‑76 (MQ=255) tGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCAg > 1:8668613/1‑76 (MQ=255) | GGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCAG > REL606/4180174‑4180324 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |