| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,145,986 | C→G | I41M (ATC→ATG) | rpoD → | RNA polymerase sigma factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,145,986 | 0 | C | G | 99.5% | 1655.7 / ‑2.3 | 377 | I41M (ATC→ATG) | rpoD | RNA polymerase sigma factor |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base G (178/197); total (178/199) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
Only 100 of 428 total aligned reads displayed.
AGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTG > REL606/3145913‑3146058 | aGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGa < 2:898978/76‑1 (MQ=255) ggAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGaa < 2:9447770/76‑1 (MQ=255) gAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAg < 1:469047/76‑1 (MQ=255) gAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAg > 2:7418055/1‑76 (MQ=255) aGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGa > 1:192228/1‑76 (MQ=255) gCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGAc > 1:5164124/1‑76 (MQ=255) gCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGAc > 1:6954130/1‑76 (MQ=255) gCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGAc > 2:7849581/1‑76 (MQ=255) cAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGAca > 2:8211593/1‑76 (MQ=255) aaGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGAcat > 2:1804616/1‑76 (MQ=255) aaGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGAcat < 1:4825318/76‑1 (MQ=255) aGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGAcatc < 1:7840490/76‑1 (MQ=255) ggCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGAcatca > 1:8662012/1‑76 (MQ=255) gCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGAcatcat < 1:3505538/76‑1 (MQ=255) gCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGAcatcat < 1:5399110/76‑1 (MQ=255) cTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACatcatc < 2:6293797/76‑1 (MQ=255) cTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACatcatc > 2:4580021/1‑76 (MQ=255) tATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATcc > 2:6141027/1‑76 (MQ=255) tATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATcc > 2:1136916/1‑76 (MQ=255) tATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATcc < 1:94387/76‑1 (MQ=255) tATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATcc < 2:2217042/76‑1 (MQ=255) tATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATcc > 1:8970185/1‑76 (MQ=255) tATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATcc > 1:3746122/1‑76 (MQ=255) aTCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCa < 2:9717950/76‑1 (MQ=255) tCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCaa < 2:8788862/76‑1 (MQ=255) cTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCaaa < 2:4167377/76‑1 (MQ=255) cTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCaaa > 1:3010272/1‑76 (MQ=255) tGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAAt < 1:6449713/76‑1 (MQ=255) tGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAAt < 1:1351574/76‑1 (MQ=255) aCCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGa > 1:5743227/1‑76 (MQ=255) ccTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGAt > 2:7065101/1‑76 (MQ=255) ccTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGAt > 1:5990409/1‑76 (MQ=255) ccTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGAt > 2:1095559/1‑76 (MQ=255) ccTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGAt < 1:9656194/76‑1 (MQ=255) cTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATc > 2:7275354/1‑76 (MQ=255) cTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATc > 1:1218648/1‑76 (MQ=255) cTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATc < 1:4300846/76‑1 (MQ=255) tATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCa < 2:9169666/76‑1 (MQ=255) tATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCa > 1:2090064/1‑76 (MQ=255) tGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAAc > 1:9905950/1‑76 (MQ=255) gCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACg < 1:5616818/76‑1 (MQ=255) gCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACg < 1:3172793/76‑1 (MQ=255) cGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGAc < 1:8447130/76‑1 (MQ=255) cGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGAc < 1:3303911/76‑1 (MQ=255) aGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACAt > 2:5536562/1‑76 (MQ=255) ggTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATg > 1:4092872/1‑76 (MQ=255) tCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATggg > 2:5455886/1‑76 (MQ=255) tCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATggg > 1:2751190/1‑76 (MQ=255) tCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATggg > 1:7570568/1‑76 (MQ=255) cAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGc < 1:6319623/76‑1 (MQ=255) aaTGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCa > 2:2491972/1‑76 (MQ=255) aTGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCAt < 1:9103300/76‑1 (MQ=255) tGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCAtt > 1:7119445/1‑76 (MQ=255) tGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCAtt < 1:8190941/76‑1 (MQ=255) gACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGa > 2:844759/1‑65 (MQ=255) gACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTc < 2:8706641/76‑1 (MQ=255) aCCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCa > 2:1277451/1‑76 (MQ=255) aCCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCa > 2:6685310/1‑76 (MQ=255) aCCATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCa < 1:4913299/76‑1 (MQ=255) ccATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAg < 1:1663700/76‑1 (MQ=255) ccATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAg < 2:4817921/76‑1 (MQ=255) ccATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAg > 1:950871/1‑76 (MQ=255) ccATCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATACAg < 2:4001138/76‑1 (MQ=255) aTCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGt < 1:5004909/76‑1 (MQ=255) tCTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTg > 2:148086/1‑76 (MQ=255) cTGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGa > 1:2328690/1‑76 (MQ=255) tGCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGAt < 2:7668601/76‑1 (MQ=255) gCCGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATg < 2:337872/76‑1 (MQ=255) ccGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATgg < 2:7731233/76‑1 (MQ=255) ccGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATgg < 2:5661480/76‑1 (MQ=255) cGGAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGga > 2:6784370/1‑76 (MQ=255) ggAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGgaa > 1:2603899/1‑76 (MQ=255) gAAGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGgaag > 2:5327911/1‑76 (MQ=255) aGATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGgaagaa > 2:8375960/1‑76 (MQ=255) gATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGaagaag < 2:9300552/76‑1 (MQ=255) gATATCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGaagaag < 2:5852089/76‑1 (MQ=255) atatCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGc > 1:10360506/1‑76 (MQ=255) atatCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGc < 2:446426/76‑1 (MQ=255) tatCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCa > 1:7275293/1‑76 (MQ=255) atCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCAc > 1:10049118/1‑76 (MQ=255) atCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCAc > 2:10147972/1‑76 (MQ=255) atCGTCGATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCAc < 2:7175274/76‑1 (MQ=255) tcgtcgATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCAcc < 1:416619/76‑1 (MQ=255) tcgtcgATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCAcc < 1:3886404/76‑1 (MQ=255) cttcgAGTCAGATCAGAGGCAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAACCACCg < 2:6850567/74‑1 (MQ=255) cgtcgATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCg < 2:3889413/76‑1 (MQ=255) cgtcgATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCg > 2:2720258/1‑76 (MQ=255) cgATTCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGAt < 2:2270110/76‑1 (MQ=255) ttCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGcc > 1:8128326/1‑76 (MQ=255) ttCAGATCAGATGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGcc > 1:5289768/1‑76 (MQ=255) tcagatgagaTGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCg < 2:5053392/76‑1 (MQ=255) tcagatcagaTGGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCg < 1:676307/76‑1 (MQ=255) agatcagatgGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCgat < 2:8062573/76‑1 (MQ=255) agatcagatgGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCgat < 1:2895765/76‑1 (MQ=255) atcagatgGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCgatga > 1:4594605/1‑76 (MQ=255) atcagaggGATGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCgatga < 2:192228/76‑1 (MQ=255) tcagatgGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCgatgat > 2:2969506/1‑76 (MQ=255) tcagatgGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCgatgat < 1:7726761/76‑1 (MQ=255) cagatgGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATc > 2:3550318/1‑76 (MQ=255) gatgGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTg < 1:8817058/76‑1 (MQ=255) | AGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTG > REL606/3145913‑3146058 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |