| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,912,192 | G→A | G24E (GGG→GAG) | ilvL → | ilvG operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,912,192 | 0 | G | A | 99.0% | 1186.4 / ‑0.1 | 300 | G24E (GGG→GAG) | ilvL | ilvG operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (167/130); total (168/132) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01 | |||||||||||
Only 100 of 310 total aligned reads displayed.
AAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCG > REL606/3912119‑3912264 | aaaTGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGc < 1:9622105/76‑1 (MQ=255) aTGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTg < 1:5947418/76‑1 (MQ=255) aTGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTg > 1:2179301/1‑76 (MQ=255) aTGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTg > 2:6973649/1‑76 (MQ=255) tGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGc > 2:703839/1‑76 (MQ=255) gACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCa < 1:9960860/76‑1 (MQ=255) cAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACt < 2:1542977/76‑1 (MQ=255) cAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACt > 2:4646818/1‑76 (MQ=255) cAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACt < 2:1404177/76‑1 (MQ=255) cAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACt < 2:7196049/76‑1 (MQ=255) cAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACt > 2:9178218/1‑76 (MQ=255) aGCCCTTCTACGAGTGCTTAGCTTGGTAGTGTTTAGAGTGGTGTTGATTATTATCCCACGGTGCTAGGCTGCAcct > 2:6009791/1‑74 (MQ=255) aGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACtt > 2:5215478/1‑76 (MQ=255) aGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACtt < 2:2179301/76‑1 (MQ=255) cccTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTgg < 1:2893820/76‑1 (MQ=255) cccTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTgg > 1:829684/1‑76 (MQ=255) cTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGAc < 1:7534390/76‑1 (MQ=255) cTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGAc < 2:5350998/76‑1 (MQ=255) cTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGAc > 2:8452794/1‑76 (MQ=255) cTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAg > 1:8362832/1‑76 (MQ=255) cTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAg > 1:5007037/1‑76 (MQ=255) cTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAg < 2:5740905/76‑1 (MQ=255) cTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAg < 2:8690133/76‑1 (MQ=255) tACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAgg < 1:5647319/76‑1 (MQ=255) tACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAgg > 2:1932338/1‑76 (MQ=255) tACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAgg > 1:383058/1‑76 (MQ=255) tACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAgg > 2:7337996/1‑76 (MQ=255) tACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAgg > 2:834199/1‑76 (MQ=255) aCGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGa > 1:7842605/1‑76 (MQ=255) aCGAGCGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGa > 2:9408193/1‑76 (MQ=255) cGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGaa < 1:489238/76‑1 (MQ=255) gAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGaaa < 2:6867609/76‑1 (MQ=255) aGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAg < 1:5346065/76‑1 (MQ=255) aGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAg < 1:3504010/76‑1 (MQ=255) aGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAg < 2:7725491/76‑1 (MQ=255) gTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAgg < 1:110781/76‑1 (MQ=255) gATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCt < 1:6652690/76‑1 (MQ=255) aTTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCtt > 2:3048956/1‑76 (MQ=255) ttAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGGCGAGGAAAGGCTTa < 1:4408574/76‑1 (MQ=255) tAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTag > 2:8083574/1‑76 (MQ=255) tAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTag > 1:9815917/1‑76 (MQ=255) aGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTaga < 2:1789307/76‑1 (MQ=255) gCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTagag > 1:7338599/1‑76 (MQ=255) ccTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAgaga > 1:6260688/1‑76 (MQ=255) cTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGAt > 1:527388/1‑76 (MQ=255) cTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGAt > 1:8441357/1‑76 (MQ=255) cTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGAt > 1:4209487/1‑76 (MQ=255) tGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATc > 2:4313261/1‑76 (MQ=255) ggTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCa > 1:10329737/1‑76 (MQ=255) ggTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCa < 1:571369/76‑1 (MQ=255) cGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGc > 1:9270415/1‑76 (MQ=255) cGTGATTAGCGTGGGGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGc > 2:3167280/1‑76 (MQ=255) gATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCtt < 2:1607199/76‑1 (MQ=255) gATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCtt < 2:3525043/76‑1 (MQ=255) ttAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTaa < 2:6405172/76‑1 (MQ=255) ttAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTaa > 2:4399463/1‑76 (MQ=255) ttAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTaa > 2:2265098/1‑76 (MQ=255) cGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGaa < 1:4961768/76‑1 (MQ=255) cGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGaa > 2:3318181/1‑76 (MQ=255) gtggtggtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAAc > 1:1427249/1‑76 (MQ=255) tggtggtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACt > 1:6962495/1‑76 (MQ=255) ggtggtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTa > 1:5108783/1‑76 (MQ=255) gtggtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTaa > 2:5922185/1‑76 (MQ=255) gtggtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTaa < 1:3577372/76‑1 (MQ=255) gtggtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTaa < 2:3378835/76‑1 (MQ=255) tggtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAg > 2:4141500/1‑76 (MQ=255) tggtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAg < 2:4726453/76‑1 (MQ=255) tggtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAg < 1:8871317/76‑1 (MQ=255) tggtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAg > 2:10358037/1‑76 (MQ=255) ggtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGa > 1:2977433/1‑76 (MQ=255) gtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGAc < 1:7215171/76‑1 (MQ=255) tgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGAcc > 2:4835383/1‑76 (MQ=255) attattATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGAcccc < 1:8704640/76‑1 (MQ=255) ttattaTCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGAccccc > 1:9152325/1‑76 (MQ=255) ttattaTCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGAccccc < 2:9013510/76‑1 (MQ=255) attatCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGc < 2:8852272/76‑1 (MQ=255) attatCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGc > 2:5444176/1‑76 (MQ=255) attatCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGc < 2:786407/76‑1 (MQ=255) attatCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGc > 2:1139580/1‑76 (MQ=255) ttatCCCACCGTGCGAGGCTGCCCTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCa > 1:2546990/1‑76 (MQ=255) ttatCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCa < 1:4668223/76‑1 (MQ=255) tatCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCAc > 1:4237850/1‑76 (MQ=255) atCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCAcc > 1:9041019/1‑76 (MQ=255) atCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCAcc > 1:1987670/1‑76 (MQ=255) atCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCAcc > 1:10001469/1‑76 (MQ=255) ccccACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCg < 1:2760852/75‑1 (MQ=255) cccACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGa > 1:6035724/1‑76 (MQ=255) cccACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGa > 2:2671806/1‑76 (MQ=255) cccACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGa > 1:6461010/1‑76 (MQ=255) cccACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGa < 1:3248205/76‑1 (MQ=255) ccACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGaa < 1:3305694/76‑1 (MQ=255) ccccGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGaaa < 1:1850632/74‑1 (MQ=255) cACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGaaa < 1:4568933/76‑1 (MQ=255) cACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGaaa > 2:4482385/1‑76 (MQ=255) aCCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAg < 1:4515420/76‑1 (MQ=255) cGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGt < 2:6609226/76‑1 (MQ=255) tGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTcc > 1:4441924/1‑76 (MQ=255) gCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCg < 2:1332359/76‑1 (MQ=255) gCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCg > 1:4120700/1‑76 (MQ=255) gCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACACCCGCACCGAAAGGTCCg > 2:2501654/1‑76 (MQ=255) | AAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCG > REL606/3912119‑3912264 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |