| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,193,779 | 0 | G | T | 99.6% | 911.0 / NA | 240 | L143I (CTT→ATT) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (79/160); minor base A (0/1); total (79/161) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 276 total aligned reads displayed.
GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA > REL606/2193706‑2193851 | cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATcccc < 1:2402870/76‑1 (MQ=255) cGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGT‑C‑GGGAAAAAGCCCCCATAACAACAAAACACCCATcccc > 1:3791320/1‑76 (MQ=255) aTGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGG‑C‑GGGAAACCCGCCCAAAAAAAGAAAAAAGCCCAAACccac > 2:5299681/1‑76 (MQ=255) tGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacc < 1:5462934/76‑1 (MQ=255) tGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacc < 2:2213191/76‑1 (MQ=255) gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacca < 2:6072670/76‑1 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAg < 1:6613156/76‑1 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAg < 2:511721/76‑1 (MQ=255) aTCCGGCGTCCAGGCGCGGCTGGGCTTGCTGC‑G‑GGGAACGAACCCCAAAAACAACGAAAACCCCACACccaccaca > 2:5637157/1‑74 (MQ=255) aTCCGGCGTCCAGGCGCGGCTGGGCTGGGTGT‑CGGGAAAACACCCCAAAAAACACCAAAACCCAAACCCcccccaa > 2:8451981/1‑75 (MQ=255) aTCCGGCGTCCAGGCGCGGCTGGGCTGGCTGG‑C‑GGGAAACAGCCCCCAAAAAAACAAAAAGCCCAACcccccccaa > 2:5582802/1‑73 (MQ=255) tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt < 2:1253506/76‑1 (MQ=255) ccGGCGTCCAGGCGCGGCTGGGCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa > 1:9028014/1‑76 (MQ=255) ggCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa < 2:2363786/76‑1 (MQ=255) ggCGTCCAGGCGCGGCTGGGCTGGCTGT‑C‑GGGAAATAGCCCCCATAACCGCAAAACGCCAACCCCCACCAGATaaa > 1:3508223/1‑76 (MQ=255) gCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg < 2:6659207/76‑1 (MQ=255) gCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg < 2:106784/76‑1 (MQ=255) gCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAAAGCCAATCCCCACCAGATAAAg > 1:4576932/1‑76 (MQ=255) cGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGc < 2:1568562/76‑1 (MQ=255) cGTCCAGGCGCGGCTGGGCTGGCTGG‑C‑GGAAAAAACCCCCAAAAAAAGCAAAAAACCAACCCCCCCCCAAAAAAGc > 2:6427896/1‑76 (MQ=255) gTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAACAAAACGCCAATCCCCCCCAGATAAAACa > 1:8464487/1‑76 (MQ=255) tCCAGGCGCGGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCAt < 2:6852409/76‑1 (MQ=255) tCCAGGCGCGGCTGGGCTGGCTGT‑C‑GGGAAACAACCCCCAAAACAGCAAAACGCCCAACCCCCCCCCATAAAACAt > 2:5435649/1‑76 (MQ=255) cAGGCGCGGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa < 2:3553564/76‑1 (MQ=255) cAGGCGCGGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa < 2:8198469/76‑1 (MQ=255) cAGGCGCGGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa < 1:1734353/76‑1 (MQ=255) aGGCGCGGCTGGTCTCGCTGTGC‑GAAAATACGCCCCAA‑AACCGGAAAACACCCAACCCCACCAGAAAAAGCAAGAc > 2:7730870/1‑76 (MQ=255) ggCGCGGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACg < 1:5750281/76‑1 (MQ=255) gcgcGGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGc < 2:101162/76‑1 (MQ=255) cgcgGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGcc < 1:9355406/76‑1 (MQ=255) gcgGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt < 1:9532547/76‑1 (MQ=255) cgGCTGGTCTGGCTGT‑G‑GGAAAACAGCCCCAAAAACAACAAAACGACAATCCCCCCCAGATAAAACATGCCGCCTg > 2:7474067/1‑76 (MQ=255) ggCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt < 1:113605/76‑1 (MQ=255) gCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa < 1:9773577/76‑1 (MQ=255) gCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa < 1:8012512/76‑1 (MQ=255) cTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt > 1:2683756/1‑76 (MQ=255) cTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt < 1:8269120/76‑1 (MQ=255) cTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt > 1:7084928/1‑76 (MQ=255) cTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt > 1:594257/1‑76 (MQ=255) tGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt < 1:1514619/76‑1 (MQ=255) tGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt < 1:1954156/76‑1 (MQ=255) tGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt > 1:3371037/1‑76 (MQ=255) ggTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa < 2:9285234/76‑1 (MQ=255) ggTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa > 1:3659500/1‑76 (MQ=255) ggTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa < 1:5331570/76‑1 (MQ=255) gTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa < 2:4404850/76‑1 (MQ=255) gTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa < 1:5555638/76‑1 (MQ=255) cTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc > 2:8175134/1‑76 (MQ=255) cTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc < 1:8141619/76‑1 (MQ=255) tGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa > 1:5187914/1‑76 (MQ=255) gCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg < 1:469941/76‑1 (MQ=255) gCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg < 1:9786340/76‑1 (MQ=255) tGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt > 1:6364678/1‑76 (MQ=255) tGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt > 1:7596001/1‑76 (MQ=255) tGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt > 1:4108034/1‑76 (MQ=255) tt‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt < 2:1798785/75‑1 (MQ=255) gT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt < 1:2301850/76‑1 (MQ=255) c‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt < 1:7385420/76‑1 (MQ=255) c‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt > 2:2649721/1‑76 (MQ=255) ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt > 2:8879567/1‑76 (MQ=255) ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt > 1:3610024/1‑76 (MQ=255) ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt > 2:4835450/1‑76 (MQ=255) aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc > 1:4355963/1‑76 (MQ=255) aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc < 2:7901418/76‑1 (MQ=255) aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc < 2:2880389/76‑1 (MQ=255) aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc < 1:7946292/76‑1 (MQ=255) aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg < 1:7160578/76‑1 (MQ=255) aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg < 2:910083/76‑1 (MQ=255) cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt > 2:2441614/1‑76 (MQ=255) gCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg < 1:3220463/76‑1 (MQ=255) gCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg < 2:3654844/76‑1 (MQ=255) ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgga > 2:5162564/1‑75 (MQ=255) ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc < 2:9028014/76‑1 (MQ=255) caccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc < 2:3339530/74‑1 (MQ=255) cccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg < 2:2030717/76‑1 (MQ=255) ccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc > 1:1401943/1‑76 (MQ=255) ccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc > 2:5865612/1‑76 (MQ=255) cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa > 1:2997629/1‑76 (MQ=255) cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa > 1:6551401/1‑76 (MQ=255) cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa < 1:2189591/76‑1 (MQ=255) cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa > 1:6270372/1‑76 (MQ=255) cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa > 1:4777017/1‑76 (MQ=255) aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt > 2:9772408/1‑76 (MQ=255) tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg < 2:5340887/76‑1 (MQ=255) tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg < 2:3242441/76‑1 (MQ=255) aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc > 2:7181718/1‑76 (MQ=255) aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc > 1:9267251/1‑76 (MQ=255) aGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa > 1:7417422/1‑76 (MQ=255) cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCagc > 1:8932709/1‑74 (MQ=255) cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc < 2:9616444/76‑1 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 2:10358690/76‑1 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa > 1:2143587/1‑76 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 2:1279531/76‑1 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 2:1752497/76‑1 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 2:4142274/76‑1 (MQ=255) aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg < 2:4713387/76‑1 (MQ=255) aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc < 2:6361345/76‑1 (MQ=255) ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCCCCAgccgcc < 1:6137328/76‑1 (MQ=255) ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc > 1:10167997/1‑76 (MQ=255) ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc > 1:10011573/1‑76 (MQ=255) | GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑C‑GGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA > REL606/2193706‑2193851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |