BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,213,060	A→T	I165N (ATC→AAC)	fruK ←	1‑phosphofructokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,213,060	0	A	T	98.7%	923.4 / NA	239	I165N (ATC→AAC)	fruK	1‑phosphofructokinase
Reads supporting (aligned to +/- strand): ref base A (0/1); major base T (112/123); minor base G (3/0); total (115/124)
Fisher's exact test for biased strand distribution p-value = 1.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

GCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTA  >  REL606/2212987‑2213132

gcggggGTTAGGTTTCCCCAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTa                                                                        <  2:1487568/76‑1 (MQ=255)

 cggcggTTAGGTTTTACCAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACCCTTCACCGCTACTATCAAAGGTaa                                                                       >  2:3664334/1‑76 (MQ=255)

 cggcggTTAGGGTTCACCAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAAATaa                                                                       >  2:2195912/1‑76 (MQ=255)

  ggcggTTAGGTTTCCCCAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAAt                                                                      <  2:6407322/76‑1 (MQ=255)

  ggcggTTAGGTTTCACCAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAAt                                                                      >  1:6149469/1‑76 (MQ=255)

   gcgtTTAGGTTTCCCCAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATg                                                                     <  2:1142026/76‑1 (MQ=255)

   gcggTTAGGTTCCCCCAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATg                                                                     <  1:967252/76‑1 (MQ=255)

    cggTTAGGTTTCACCAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGc                                                                    >  1:1526878/1‑76 (MQ=255)

      gTTAGTTTTCCCCAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCaa                                                                  <  1:9812673/76‑1 (MQ=255)

       ttgggTTTCCCCAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAg                                                                 <  2:4183796/73‑1 (MQ=255)

       ttAGTTTCCCCCAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAg                                                                 <  2:8577083/76‑1 (MQ=255)

       ttAGGTTTCACCAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAg                                                                 >  2:5164485/1‑76 (MQ=255)

        tgggTTTCCCCAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAgg                                                                <  2:3155128/74‑1 (MQ=255)

         agttttCCCCAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGa                                                               <  1:8607353/73‑1 (MQ=255)

         aGGTTTCACCAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGa                                                               >  2:9862484/1‑76 (MQ=255)

         aGGTTTCACCAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGa                                                               >  1:2553960/1‑76 (MQ=255)

          ggTTCCCCCAGCCACGGTGCCGCT‑TTCAACCCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGac                                                              <  1:8361382/76‑1 (MQ=255)

            tttCCCCAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGacac                                                            <  2:7387455/76‑1 (MQ=255)

             ttCCCCAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACt                                                           <  1:8405450/76‑1 (MQ=255)

             ttCACCAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACt                                                           >  1:5483530/1‑76 (MQ=255)

               ccccAGCCACGGTTCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGa                                                         <  1:6537307/74‑1 (MQ=255)

                cccAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGAc                                                        <  2:4325874/75‑1 (MQ=255)

                 ccAGCCACGGTTCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACt                                                       <  2:8977289/76‑1 (MQ=255)

                 ccAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACt                                                       <  2:7796287/76‑1 (MQ=255)

                  cAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTa                                                      >  2:4852308/1‑76 (MQ=255)

                  cAGCCAC‑GTGCCCCTGTTCCAACCGGCTACCAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTa                                                      <  2:6138281/76‑1 (MQ=255)

                   aGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTAc                                                     <  1:4530020/76‑1 (MQ=255)

                    gCCAGGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACg                                                    <  1:9030587/76‑1 (MQ=255)

                    gCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACg                                                    <  2:5407175/76‑1 (MQ=255)

                     ccACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGc                                                   >  2:7687660/1‑76 (MQ=255)

                      cACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCa                                                  <  1:7633850/76‑1 (MQ=255)

                      cACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCa                                                  <  2:4482541/76‑1 (MQ=255)

                       aCGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAg                                                 <  1:7844184/76‑1 (MQ=255)

                        cGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAgg                                                >  1:5967136/1‑76 (MQ=255)

                           tGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGa                                             >  1:8457660/1‑76 (MQ=255)

                              cGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAggc                                          >  2:2718758/1‑74 (MQ=255)

                              cGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTc                                          <  2:8524440/76‑1 (MQ=255)

                              cGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTc                                          <  1:10256046/76‑1 (MQ=255)

                               gCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCa                                         <  2:6203971/76‑1 (MQ=255)

                                cT‑TTCAACCCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCAt                                        <  1:2013442/76‑1 (MQ=255)

                                cT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCAt                                        <  2:1927476/76‑1 (MQ=255)

                                 t‑ttCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATc                                       >  1:3374986/1‑76 (MQ=255)

                                 t‑ttCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGGCATc                                       >  2:8022420/1‑76 (MQ=255)

                                   ttCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATcc                                      >  1:5030361/1‑76 (MQ=255)

                                   ttCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATcc                                      >  1:7088289/1‑76 (MQ=255)

                                    tCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCa                                     <  2:9598074/76‑1 (MQ=255)

                                    tCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCa                                     <  2:8800225/76‑1 (MQ=255)

                                    tCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCa                                     <  1:2380809/76‑1 (MQ=255)

                                    tCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCa                                     <  1:4066643/76‑1 (MQ=255)

                                      aacccTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGt                                   <  1:5426369/73‑1 (MQ=255)

                                      aaaCCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTAc                                                     >  1:8890380/1‑58 (MQ=255)

                                      aaaCCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGt                                   >  2:1983510/1‑76 (MQ=255)

                                      aaaCCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGt                                   <  1:4857264/76‑1 (MQ=255)

                                      aaaCCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGt                                   <  2:1853869/76‑1 (MQ=255)

                                       aaCCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTc                                  >  1:9185917/1‑76 (MQ=255)

                                        aCCTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCg                                 >  1:6078908/1‑76 (MQ=255)

                                         ccTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCgg                                >  1:5185913/1‑76 (MQ=255)

                                         ccTGCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGGCgg                                >  2:7831577/1‑76 (MQ=255)

                                            gCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGt                                           <  2:3337963/62‑1 (MQ=255)

                                            gCTACTAACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGGGa                             >  2:6996952/1‑76 (MQ=255)

                                            gCTACTAACGCTTCACGGCTACTATCAAAGGTAATGCAAGGACACTGACTACGCAGGCGAGGCAACCAGTCGGTGa                             >  2:6590373/1‑76 (MQ=255)

                                              tactaACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGGAc                           >  1:7985753/1‑76 (MQ=255)

                                              tactaACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGGCATCCAGTCGGTGAAc                           >  1:2949614/1‑76 (MQ=255)

                                               actaACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACg                          >  2:9251498/1‑76 (MQ=255)

                                                ctaACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGc                         >  1:3188990/1‑76 (MQ=255)

                                                 taACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCt                        <  1:7429555/76‑1 (MQ=255)

                                                 taACGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCt                        <  1:2872388/76‑1 (MQ=255)

                                                  aaCGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCtt                       >  1:9419841/1‑76 (MQ=255)

                                                  aaCGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCtt                       <  2:1647665/76‑1 (MQ=255)

                                                   aCGCTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTc                      <  1:3965448/76‑1 (MQ=255)

                                                     gCTTCACGGCTACTATCAAAGTTAATGCAAGGACAATGACTAAGCCGGCGAGTCACCCAGTCGGTGAAAGCTTccc                    >  2:1396103/1‑75 (MQ=255)

                                                      cTTCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCgg                   <  2:8273533/76‑1 (MQ=255)

                                                       ttCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGGACGCTTTCggg                  >  2:2455141/1‑76 (MQ=255)

                                                       ttCACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCggg                  <  1:6775490/76‑1 (MQ=255)

                                                         cACGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCt                <  2:3372983/76‑1 (MQ=255)

                                                         cACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCt                <  2:5917397/76‑1 (MQ=255)

                                                          aCGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTg               <  1:7185029/76‑1 (MQ=255)

                                                          aCGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTg               <  2:7623582/76‑1 (MQ=255)

                                                          aCGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGGGAACGCTTCCGGGCTg               >  1:958470/1‑76 (MQ=255)

                                                           cGGCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGa              <  2:3941254/76‑1 (MQ=255)

                                                             gCTACTATCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACg            >  2:7946708/1‑76 (MQ=255)

                                                              ctactaTCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGc           >  2:2741775/1‑76 (MQ=255)

                                                              ctactaTCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGc           >  1:5252250/1‑76 (MQ=255)

                                                              ctactaTCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGc           >  2:9040885/1‑76 (MQ=255)

                                                              ctactaTCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGc           <  1:9923832/76‑1 (MQ=255)

                                                               tactaTCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGcc          >  2:7131089/1‑76 (MQ=255)

                                                               tactaTCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGcc          >  2:202708/1‑76 (MQ=255)

                                                               tactaTCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGcc          >  1:8150989/1‑76 (MQ=255)

                                                               tactaTCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGcc          >  1:9454863/1‑76 (MQ=255)

                                                                actaTCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCt         >  2:9234378/1‑76 (MQ=255)

                                                                 ctaTCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTg        <  2:9428143/76‑1 (MQ=255)

                                                                 ctaTCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTg        >  1:10205641/1‑76 (MQ=255)

                                                                  taTCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGa       >  2:4546223/1‑76 (MQ=255)

                                                                  taTCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGGGAACGCTTCCGGGCTGACGCCTGa       >  1:2236343/1‑76 (MQ=255)

                                                                   aTCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGAc      <  2:8194616/76‑1 (MQ=255)

                                                                   aTCAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGCCATCCAGTCAGTGAACGCTTCCGGGCTGACGCCTGAc      >  1:3733164/1‑76 (MQ=255)

                                                                     cAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACgg    >  1:1830075/1‑76 (MQ=255)

                                                                     cAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACgg    >  1:122833/1‑76 (MQ=255)

                                                                     cAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACgg    >  2:3008953/1‑76 (MQ=255)

                                                                     cAAAGTTAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACgg    >  1:3774347/1‑76 (MQ=255)

GCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCT‑TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTA  >  REL606/2212987‑2213132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`