| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,250,824 | T→C | H640R (CAC→CGC) | napA ← | nitrate reductase, periplasmic, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,250,824 | 0 | T | C | 99.5% | 820.9 / ‑5.0 | 200 | H640R (CAC→CGC) | napA | nitrate reductase, periplasmic, large subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (91/108); total (91/109) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
Only 100 of 268 total aligned reads displayed.
TTACCGTTCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGT > REL606/2250752‑2250899 | ttACCGTTCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATcgtgaccgcgac < 2:706578/76‑1 (MQ=255)ttACCGTTCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGGGCCCGATcgtgaacgcgac > 1:3460828/1‑76 (MQ=255) tACCGTTCACCACCGGCCAGCGCAGACCGCGCGCTTTTTGGTAGTCATCGAACGGGGGCCGATCgtgaccccgacc > 2:3219257/1‑76 (MQ=255) tACCGTTCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCgtgaccgcgacc < 2:7770117/76‑1 (MQ=255) aCCGTTCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGtgaccgcgaccg < 1:3618249/76‑1 (MQ=255) ccGTTCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCCTCGAACGGTGCCAGGTCGtgaccgcgagcgg > 1:7284274/1‑75 (MQ=255) cGTTCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGaccgcgaccgcg < 1:8294748/76‑1 (MQ=255) gTTCACCACCGGCCAGCGCAGCCCGCGCGCTTTGTGGTAGTCATCGCACGGTGCCAGAACCTGGCCCCGGCCGCGa > 2:2372998/1‑76 (MQ=255) gTTCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGaccgcgaccgcgc > 1:6657756/1‑75 (MQ=255) gTTCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGAccgcgaccgcga > 1:1839755/1‑76 (MQ=255) ttCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGGGCCAGATCGTGACcgcgaccgcgac > 1:9623546/1‑76 (MQ=255) ttCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGGCATCGAAAGGGGCCAGATCGTGACcgcgaccgcgac > 2:6295974/1‑76 (MQ=255) caccacCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCa < 2:5291667/76‑1 (MQ=255) caccacCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCa < 1:5934167/76‑1 (MQ=255) cccaccGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCaa < 1:9913432/75‑1 (MQ=255) ccaccGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCaaa < 1:4690841/76‑1 (MQ=255) accGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAAcc < 2:7364467/76‑1 (MQ=255) accGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAAcc < 2:8852462/76‑1 (MQ=255) ccGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCa < 1:2578812/76‑1 (MQ=255) ccGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCa > 2:1456320/1‑76 (MQ=255) ccGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCCTGACCGCGACCGCGACCAAACCa > 1:6138179/1‑76 (MQ=255) cGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGCCCAAACCAt > 1:5696366/1‑76 (MQ=255) cGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCAt < 2:6056828/76‑1 (MQ=255) ggCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATg > 2:3322110/1‑76 (MQ=255) gcccAGCGCGAACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGAGCGTGACCGCGACCGCGACCAAACCATg < 1:4264992/74‑1 (MQ=255) gCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGc > 1:4573602/1‑76 (MQ=255) ccAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCg < 2:3744898/76‑1 (MQ=255) ccAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCg < 1:631758/76‑1 (MQ=255) ccAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCg < 2:5896518/76‑1 (MQ=255) cAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGt > 2:4928900/1‑76 (MQ=255) cAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGt > 2:4724244/1‑76 (MQ=255) cAGAGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGt > 2:8063608/1‑76 (MQ=255) aGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTa > 2:1943033/1‑76 (MQ=255) aGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTa < 2:697191/76‑1 (MQ=255) aGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACAATGCGTa > 2:4444985/1‑76 (MQ=255) gcgcAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTAc > 1:9309988/1‑76 (MQ=255) gcgcAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTAc < 1:1133083/76‑1 (MQ=255) cgcAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTAct > 1:1296132/1‑76 (MQ=255) cgcAGACCGCGCGCTTTGTGCTAGTCATCGAACGGTGCCAGATCGTGACCGCGCCCGCGACCAAACCATGCGTAct < 1:3762300/76‑1 (MQ=255) cgcAGACCGCGCGCTTGGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTAct < 1:6620204/76‑1 (MQ=255) gcAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGAACCAACCATGAGTActc > 2:9426091/1‑76 (MQ=255) cAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTActct < 2:10333519/76‑1 (MQ=255) cAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACAAAACCATGCGTActct > 2:5069853/1‑76 (MQ=255) aGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCtt > 2:6021387/1‑76 (MQ=255) gACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTc > 2:4573271/1‑76 (MQ=255) gACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTc < 1:9734193/76‑1 (MQ=255) cgcgcgCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGaa < 1:2716153/76‑1 (MQ=255) cgcgcTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACa > 2:2834048/1‑76 (MQ=255) cgcgcTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACa < 2:1284088/76‑1 (MQ=255) cgcgcTTGATAGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAAcg < 2:5723511/76‑2 (MQ=255) gcgcTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAg < 1:5065651/76‑1 (MQ=255) cgcTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGc > 2:6878886/1‑76 (MQ=255) cTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGccc > 2:3645094/1‑76 (MQ=255) cTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGccc < 2:2952398/76‑1 (MQ=255) tttGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCt > 1:7453259/1‑76 (MQ=255) ttGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCtt < 2:2916911/76‑1 (MQ=255) tgGTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCttttt < 2:5801747/76‑1 (MQ=255) ggTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTg < 1:10343352/76‑1 (MQ=255) ggTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTg > 2:9768324/1‑76 (MQ=255) ggTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTg < 1:35348/76‑1 (MQ=255) ggTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTg < 1:5409448/76‑1 (MQ=255) ggTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTg < 1:8379038/76‑1 (MQ=255) gTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGc < 2:10138254/76‑1 (MQ=255) gTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGc < 2:524293/76‑1 (MQ=255) gTAGTCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGc > 1:8720745/1‑76 (MQ=255) gTAGTCATCGAACGGTACCAGATCGTGCCCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGc < 1:7909554/76‑1 (MQ=255) tCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGAt < 1:2026811/76‑1 (MQ=255) tCATCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGAt < 1:8063608/76‑1 (MQ=255) aTCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAg > 1:8869196/1‑76 (MQ=255) aTCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAg < 1:5901665/76‑1 (MQ=255) aTCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAg < 2:9112568/76‑1 (MQ=255) aTCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAg < 1:605065/76‑1 (MQ=255) aTCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAg > 2:9598542/1‑76 (MQ=255) tCGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGa < 2:7984106/76‑1 (MQ=255) cGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGaa < 2:40908/76‑1 (MQ=255) cGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGaa < 2:2133935/76‑1 (MQ=255) cGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGaa > 2:8128303/1‑76 (MQ=255) cGAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGaa > 2:3829568/1‑76 (MQ=255) gAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAg > 1:9652314/1‑76 (MQ=255) gAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAg < 2:4306715/76‑1 (MQ=255) gAACGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAg > 2:7683248/1‑76 (MQ=255) aaCGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAGc > 2:6577760/1‑76 (MQ=255) aCGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAGcc < 1:7484758/76‑1 (MQ=255) aCGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAGcc < 2:6717182/76‑1 (MQ=255) cGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAGccc > 1:6971379/1‑76 (MQ=255) cGGTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAGccc < 1:906214/76‑1 (MQ=255) cGGGGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAGccc < 1:6525112/76‑1 (MQ=255) gTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAg < 1:6431179/76‑1 (MQ=255) gTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAg > 1:2936547/1‑76 (MQ=255) gTGCCAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAg > 1:4453579/1‑76 (MQ=255) cAGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTcg > 1:8209295/1‑76 (MQ=255) aGATCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGaa < 1:4772233/65‑1 (MQ=255) tCGTGACCGCGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCggg > 1:242051/1‑76 (MQ=255) gtgaccgcgaccGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGAt > 2:7931928/1‑76 (MQ=255) gaccgcgaccgcGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTc > 2:2587426/1‑76 (MQ=255) gaccgcgaccgcGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTc > 1:2345336/1‑76 (MQ=255) gaccgcgaccgcGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTc > 2:4773948/1‑76 (MQ=255) gaccgcgaccgcGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTc > 2:335435/1‑76 (MQ=255) accgcgaccgcgACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCa > 1:4102801/1‑76 (MQ=255) ccgcgaccgcgaCCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCAt < 1:1511690/76‑1 (MQ=255) | TTACCGTTCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGT > REL606/2250752‑2250899 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |