| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 79,133 | (T)5→4 | coding (971/1656 nt) | yabN ← | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 79,129 | 0 | T | . | 99.3% | 720.6 / ‑2.1 | 147 | coding (975/1656 nt) | yabN | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base . (67/79); total (68/79) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
Only 100 of 164 total aligned reads displayed.
CTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCA > REL606/79054‑79200 | cTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAAActct > 2:2867707/1‑76 (MQ=255) tGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTCGCAACCTTCCTCCAGGCGGCGTTCAATCTCtt > 2:3977567/1‑76 (MQ=255) tGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGGTTTCAATCTCtt > 1:3729300/1‑76 (MQ=255) gATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCttt < 1:3979214/76‑1 (MQ=255) aTTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCtttt < 2:6039712/76‑1 (MQ=255) aTTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCtttt > 2:5931139/1‑76 (MQ=255) aTTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCtttt > 2:3851531/1‑76 (MQ=255) ttCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCCATCTCttttc > 1:3587542/1‑75 (MQ=255) tCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCttttcc > 1:289372/1‑74 (MQ=255) cccGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCAc < 2:2249926/76‑1 (MQ=255) gcgATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCt < 1:1358733/76‑1 (MQ=255) gcgATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCt > 1:2654795/1‑76 (MQ=255) cgATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCtg < 2:1732423/76‑1 (MQ=255) gATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCtgt > 1:2374821/1‑76 (MQ=255) gATGGGTGCGGCTGTCGAATAGTAAATAGGAGCAACCTTCCTCCAGGCGGCTTTCAATATCTTTTT‑CTCACCCcgt > 2:1759199/1‑76 (MQ=255) gATGGGTGCGGCTGGCGAATAGTAAAAAGTAGCAACCTTCCTCCAGGCGGCGTTCAATCTC‑TTTTCCTCCCCCtct > 2:1617433/1‑74 (MQ=255) aTGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCtgtg < 1:798909/76‑1 (MQ=255) aTGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCtgtg < 2:4930025/76‑1 (MQ=255) aTGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCtgtg > 1:3220776/1‑76 (MQ=255) aTGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCtgtg < 1:1895128/76‑1 (MQ=255) tGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTgg > 1:3146135/1‑76 (MQ=255) tGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTgg < 1:3461722/76‑1 (MQ=255) tGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTgg < 2:3837831/76‑1 (MQ=255) tGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTgg < 1:5108597/76‑1 (MQ=255) ggTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGAc < 1:3024753/76‑1 (MQ=255) ggTGCGGCTGGCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGGTTTCAATCTCTTTTT‑CTCACCCTGTGGAc > 2:3170165/1‑76 (MQ=255) gTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGAcc > 1:7556/1‑76 (MQ=255) gCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGGTTTCAATCTC‑TTTTCCTCACCCTGTGGACCtt > 2:4222280/1‑76 (MQ=255) gCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCCCACCCTCTGGACCtt > 2:6293692/1‑76 (MQ=255) ggCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCtttt < 2:2687786/76‑1 (MQ=255) ggCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCtttt < 2:3989781/76‑1 (MQ=255) ggCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCtttt < 1:4248213/76‑1 (MQ=255) ggCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCtttt < 2:5345454/76‑1 (MQ=255) ggCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCtttt < 2:4129407/76‑1 (MQ=255) cTGTGGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCTCCCTGTGGACCTTTTAg < 2:5212014/76‑1 (MQ=255) cTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAg < 1:2128451/76‑1 (MQ=255) cTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAg > 2:3427313/1‑76 (MQ=255) cTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAg > 1:5667686/1‑76 (MQ=255) tGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGc > 1:4081647/1‑76 (MQ=255) tGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGc > 1:3626761/1‑76 (MQ=255) gTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCa > 2:6005871/1‑76 (MQ=255) tCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCAt > 1:2247681/1‑76 (MQ=255) tCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCAt < 1:1002103/76‑1 (MQ=255) tCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCAt < 1:2484618/76‑1 (MQ=255) tCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCAt < 2:2642479/76‑1 (MQ=255) tCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCAt > 2:5494036/1‑76 (MQ=255) tCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCAt > 1:2263342/1‑76 (MQ=255) tCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCAt < 1:4868810/76‑1 (MQ=255) cGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATc < 2:3692412/76‑1 (MQ=255) gAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCa < 2:4368803/76‑1 (MQ=255) aatagtaaatagtaGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAg < 1:3846770/76‑1 (MQ=255) aatagtaaatagtaGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAg < 2:5284859/76‑1 (MQ=255) atagtaaatagtaGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGa > 2:4764021/1‑76 (MQ=255) atagtaaatagtaGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGa > 1:1023755/1‑76 (MQ=255) atagtaaatagtaGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGa < 1:5773911/76‑1 (MQ=255) tagtaaatagtaGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGAc > 1:6172968/1‑76 (MQ=255) tagtaaatagtaGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGAc > 2:1981182/1‑76 (MQ=255) agtaaatagtaGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGAcc > 2:1854227/1‑76 (MQ=255) agtaaatagtaGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGAcc < 2:190891/76‑1 (MQ=255) gtaaatagtaGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCt < 1:2458558/76‑1 (MQ=255) gtaaatagtaGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCt < 1:976813/76‑1 (MQ=255) taaatagtaGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTc < 2:1584441/76‑1 (MQ=255) taaatagtaGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTc < 1:4708666/76‑1 (MQ=255) taaatagtaGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTc > 1:4720068/1‑76 (MQ=255) aatagtaGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCa < 1:2689308/76‑1 (MQ=255) aatagtaGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCa < 1:5244566/76‑1 (MQ=255) aatagtaGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCa > 1:309896/1‑76 (MQ=255) atagtaGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAg > 1:4808562/1‑76 (MQ=255) atagtaGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAg > 2:2415077/1‑76 (MQ=255) atagtaGCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAg < 2:529799/76‑1 (MQ=255) gtagCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTg > 1:6025795/1‑76 (MQ=255) tagCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTgg > 2:4266258/1‑76 (MQ=255) tagCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTgg < 2:1046014/76‑1 (MQ=255) tagCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTgg > 2:1321006/1‑76 (MQ=255) agCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGCCCTCCAGCTGGc > 2:1638769/1‑76 (MQ=255) agCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGc < 1:4541039/76‑1 (MQ=255) agCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGc < 2:4426301/76‑1 (MQ=255) gCAACCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCt < 2:3614848/76‑1 (MQ=255) aCCTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCtcgt < 1:1625628/76‑1 (MQ=255) ccTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCtcgtc < 1:500401/76‑1 (MQ=255) ccTTCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCtcgtc < 2:5887217/76‑1 (MQ=255) ttCCTCCAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCgg > 2:4246270/1‑76 (MQ=255) cctccAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGCCGGCa > 1:6243364/1‑76 (MQ=255) ccAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGGaaat > 2:4848862/1‑74 (MQ=255) ccAGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAAtt < 1:2795900/76‑1 (MQ=255) aGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTc > 2:5085903/1‑76 (MQ=255) aGGCGGCTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTc > 1:1104127/1‑76 (MQ=255) ggcggcTTTCAATCGC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTcc < 2:1487149/76‑1 (MQ=255) ggcTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGc < 2:1236251/76‑1 (MQ=255) cTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGGCCTCCAGCTGGCTCGTCGGCAATTTCCGGCAg < 1:5501117/76‑1 (MQ=255) cTTTCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAg < 1:659147/76‑1 (MQ=255) tttCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGa > 1:4090563/1‑76 (MQ=255) ttCANTCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCCGAAAGTTTCGGCAGaa > 1:3173083/1‑76 (MQ=255) ttCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGaa > 2:4709763/1‑76 (MQ=255) ttCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGaa < 1:5033342/76‑1 (MQ=255) ttCAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGaa < 1:1473116/76‑1 (MQ=255) cAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAAcc < 1:4322709/76‑1 (MQ=255) cAATCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCCTCGGCCATTTCCCGCAGaaac > 1:2734839/1‑74 (MQ=255) aTCTC‑TTTTCCTCTCCCTGTGGCCCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGACCCCa < 1:1182042/76‑1 (MQ=255) aTCTC‑TTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCa < 1:4625629/76‑1 (MQ=255) | CTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCA > REL606/79054‑79200 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |