| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,002,938 | (A)8→7 | intergenic (‑137/+48) | aspC ← / ← ompF | aspartate aminotransferase, PLP‑dependent/outer membrane porin 1a (Ia;b;F) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,002,931 | 0 | A | . | 97.2% | 833.9 / 16.5 | 177 | intergenic (‑130/+55) | aspC/ompF | aspartate aminotransferase, PLP‑dependent/outer membrane porin 1a (Ia;b;F) |
| Reads supporting (aligned to +/- strand): ref base A (4/1); new base . (81/91); total (85/92) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01 | |||||||||||
Only 100 of 209 total aligned reads displayed.
ATTTACAAAATTCCAACTATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTATAAAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGA > REL606/1002856‑1003002 | aTTTACAAAATTCCAACTATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGtata < 2:3904204/76‑1 (MQ=255)aTTTACAAAATTCCAACTATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGtata > 2:3172738/1‑76 (MQ=255) tttACAAAATTCCAACTATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTATaa < 1:2303672/76‑1 (MQ=255) ttACAAAATTCCAACTATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTATaaa < 2:2899904/76‑1 (MQ=255) ttACAAAATTCCAACTATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTATaaa < 2:2628428/76‑1 (MQ=255) tACAAAATTCCAACTATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGATGGTATaaaa > 1:2352847/1‑76 (MQ=255) tACAAAATTCCAACTATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTATaaaa > 2:4899154/1‑76 (MQ=255) tACAAAATTCCAACTATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTATaaaa > 2:516460/1‑76 (MQ=255) cAAAATTCCAACTATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTATaaaaaa < 2:5261885/76‑1 (MQ=255) aaaTTCCAACTATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTATaaaaaaac > 1:6046746/1‑75 (MQ=255) aaaTTCCAACTATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTATaaaaaaac < 2:1368031/76‑2 (MQ=255) aaTTCCAACTATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTATaaaaaaaca < 2:3422844/76‑3 (MQ=255) cAACTATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGAcc < 1:4645098/76‑1 (MQ=255) cAACTATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGAcc > 2:2405710/1‑76 (MQ=255) aaCTATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCa > 1:1094201/1‑76 (MQ=255) aCTATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTATAAAAAAAACAGGACCa > 2:158950/1‑76 (MQ=255) aCTATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCaa < 1:1889447/76‑1 (MQ=255) cTATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCaaa < 1:566605/76‑1 (MQ=255) cTATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCaaa > 1:929982/1‑76 (MQ=255) tATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAg > 1:3885137/1‑76 (MQ=255) tATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAg > 1:3370147/1‑76 (MQ=255) aTTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGt > 1:6227477/1‑76 (MQ=255) ttACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAACACAGGACCAAAGTc > 2:4448028/1‑76 (MQ=255) tACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTcc > 2:856504/1‑76 (MQ=255) tACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTcc > 2:3518179/1‑76 (MQ=255) aCTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCt < 1:6275126/76‑1 (MQ=255) aCTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCt > 1:4180124/1‑76 (MQ=255) aCTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCt < 2:4057953/76‑1 (MQ=255) cTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTg < 2:589405/76‑1 (MQ=255) cTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTg > 2:2743363/1‑76 (MQ=255) tgatgaAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGt > 1:5026765/1‑76 (MQ=255) tgatgaAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGt > 2:3218444/1‑76 (MQ=255) tgatgaAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGt < 2:2877691/76‑1 (MQ=255) gatgaAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGtt < 2:386153/76‑1 (MQ=255) gatgaAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGtt > 1:1790143/1‑76 (MQ=255) gatgaAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGtt > 2:3352742/1‑76 (MQ=255) atgaAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGttt < 2:4818038/76‑1 (MQ=255) atgaAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGttt > 1:1710797/1‑76 (MQ=255) tgaAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGtttt < 2:1976162/76‑1 (MQ=255) tgaAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGtttt < 2:2896565/76‑1 (MQ=255) tgaAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGtttt > 2:5985083/1‑76 (MQ=255) gaAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGttttt < 2:4026909/76‑1 (MQ=255) gaAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGttttt < 1:3441664/76‑1 (MQ=255) gaAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGttttt < 2:6088278/76‑1 (MQ=255) gaAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGttttt > 1:5619501/1‑76 (MQ=255) aaaaCTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGtttttt < 2:1787926/76‑1 (MQ=255) aaaaCTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGtttttt < 2:2053935/76‑1 (MQ=255) aaaaCTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGtttttt < 2:3288696/76‑1 (MQ=255) aCTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCgg < 2:2352847/76‑1 (MQ=255) aCTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCgg > 1:5822004/1‑76 (MQ=255) tCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCa > 1:499680/1‑76 (MQ=255) tCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCa > 2:767397/1‑76 (MQ=255) cAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCAt > 2:4675519/1‑76 (MQ=255) cAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCAt > 2:3361365/1‑76 (MQ=255) ggCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCAttt < 2:5668627/76‑1 (MQ=255) ggtGTTTTTTCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTa < 2:4705770/74‑1 (MQ=255) cTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTaa > 2:507075/1‑76 (MQ=255) cTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTaa > 1:1319503/1‑76 (MQ=255) cTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTaa < 2:5234436/76‑1 (MQ=255) tGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAAc < 2:4411066/76‑1 (MQ=255) tGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAAc < 2:5011827/76‑1 (MQ=255) tGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAAc < 1:5380490/76‑1 (MQ=255) tGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAAc > 2:5319951/1‑76 (MQ=255) gTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACa < 1:1492484/76‑1 (MQ=255) ttttGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACaaa < 2:416239/76‑1 (MQ=255) ttttGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACaaa < 2:579507/76‑1 (MQ=255) tttGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAag < 2:1260794/76‑1 (MQ=255) tttGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAag > 1:6120004/1‑76 (MQ=255) tttGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAag > 2:5999982/1‑76 (MQ=255) tttGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAag < 1:2434764/76‑1 (MQ=255) ttGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAaga < 2:1597352/76‑1 (MQ=255) ttGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAaga > 1:609508/1‑76 (MQ=255) cAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGgt > 2:898618/1‑76 (MQ=255) aCGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGc > 2:635522/1‑76 (MQ=255) cGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCt > 1:1376395/1‑76 (MQ=255) cGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCt > 2:2227323/1‑76 (MQ=255) tGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTAt < 2:18889/76‑1 (MQ=255) gagaTTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTAtt < 2:4503237/76‑1 (MQ=255) gagaTTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTAtt < 1:1210847/76‑1 (MQ=255) gagaTTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTAtt > 1:5670033/1‑76 (MQ=255) gagaTTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTAtt < 1:5962293/76‑1 (MQ=255) agaTTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTa < 1:2158029/76‑1 (MQ=255) gaTTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAg > 1:5157931/1‑76 (MQ=255) gaTTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAg < 1:4412347/76‑1 (MQ=255) ttGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGaa < 1:3750343/76‑1 (MQ=255) gtGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGaa < 2:3715855/75‑1 (MQ=255) gCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACt > 2:1165976/1‑76 (MQ=255) ctctGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTg > 1:4659401/1‑76 (MQ=255) ctctGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTg > 2:572525/1‑76 (MQ=255) tGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTa < 1:3951135/76‑1 (MQ=255) ggAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTaa < 2:4277599/76‑1 (MQ=255) ggAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTaa > 2:1754285/1‑76 (MQ=255) ggAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTaa > 1:2628588/1‑76 (MQ=255) ggAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTaa > 1:3070376/1‑76 (MQ=255) ggAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTaa > 2:112190/1‑76 (MQ=255) gAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTaaa < 1:5297424/76‑1 (MQ=255) gAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTaaa > 2:2959890/1‑76 (MQ=255) gAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTaaa < 2:1695028/76‑1 (MQ=255) aGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACt < 1:4096166/68‑1 (MQ=255) ggTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGa > 1:3819623/1‑76 (MQ=255) | ATTTACAAAATTCCAACTATTACTGATGAAAACTCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTATAAAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGA > REL606/1002856‑1003002 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |