| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,183,400 | (T)8→7 | coding (27/633 nt) | ycfQ ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,183,393 | 0 | T | . | 98.9% | 449.3 / ‑2.0 | 93 | coding (34/633 nt) | ycfQ | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base . (28/64); total (28/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
AACCGTGTTGCCAGAACAATTTCATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTAT > REL606/1183318‑1183464 | aaCCGTGTTGCCAGAACAATTTCATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCt > 1:5674531/1‑76 (MQ=255) ccGTGTTGCCAGAACAATTTCATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCttt > 1:446847/1‑76 (MQ=255) cGTGTTGCCAGAACAATTTCATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCtttt < 2:5659885/76‑1 (MQ=255) gtgtTGCCAGAACAATTTCATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCttttt > 2:5776470/1‑76 (MQ=255) tgtTGCCAGAACAATTTCATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCtttttt > 1:5448462/1‑76 (MQ=255) gtTGCCAGAACAATTTCATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCttttttt < 2:4208878/76‑1 (MQ=255) gtTGCCAGAACAATTTCATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCttttttt > 1:2525127/1‑76 (MQ=255) tGCCAGAACAATTTCATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCtttttttac > 2:2608055/1‑74 (MQ=255) gCCAGAACAATTTCATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCtttttttaca < 2:5255581/76‑4 (MQ=255) aGAACAATTTCATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACAt > 1:4588312/1‑76 (MQ=255) aGAACAATTTCATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACAt > 2:6157886/1‑76 (MQ=255) aGAACAATTTCATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACAt > 2:2676610/1‑76 (MQ=255) aGAACAATTTCATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACAt < 1:732717/76‑1 (MQ=255) aGAACAATTTCATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACAt < 2:2227271/76‑1 (MQ=255) aaCAATTTCATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATtg < 1:5652853/76‑1 (MQ=255) cAATTTCATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATtgtg > 2:2988636/1‑76 (MQ=255) aaTTTCATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTgtgt < 2:3743644/76‑1 (MQ=255) ttCATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGa < 1:3293097/76‑1 (MQ=255) cATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGt < 1:1029756/76‑1 (MQ=255) cATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGt < 1:1458145/76‑1 (MQ=255) cATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGt < 1:1081713/76‑1 (MQ=255) ggCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAg < 1:4595406/76‑1 (MQ=255) ggCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAg < 1:4424884/76‑1 (MQ=255) ggCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAg > 2:4905843/1‑76 (MQ=255) ggCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAg > 1:2302578/1‑76 (MQ=255) ggCCTTATCAAGCGCGGAATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAg < 1:733496/76‑1 (MQ=255) ccTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGtt < 2:687961/76‑1 (MQ=255) ccTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGtt < 1:3676096/76‑1 (MQ=255) cattATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGtt < 2:5156466/74‑1 (MQ=255) cTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTg < 2:2246230/76‑1 (MQ=255) ttATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGc < 2:6120837/76‑1 (MQ=255) tATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACGTTGTGTTGAGTCAGTTGcc > 2:5334315/1‑76 (MQ=255) tATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGcc > 2:4848205/1‑76 (MQ=255) aTCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCa > 1:4435438/1‑76 (MQ=255) tCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCat < 2:2092543/76‑1 (MQ=255) tCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCat > 1:5348289/1‑76 (MQ=255) cAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCatg < 1:1889273/76‑1 (MQ=255) aaGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCatga > 2:2065698/1‑76 (MQ=255) aaGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCatga > 2:237809/1‑76 (MQ=255) aaGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCatga > 1:1157238/1‑76 (MQ=255) aaGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCatga > 2:342908/1‑76 (MQ=255) gcgcGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCatgatg < 1:3932059/76‑1 (MQ=255) gcgcGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCatgatg < 2:1892327/76‑1 (MQ=255) gcgcGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCatgatg < 2:1397936/76‑1 (MQ=255) cgcgGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGc > 2:3997621/1‑76 (MQ=255) cgcgGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGc < 1:4119302/76‑1 (MQ=255) cgGCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCg > 2:720267/1‑76 (MQ=255) ggCATCCCTGTCGAACACTTCTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGt > 2:1099823/1‑76 (MQ=255) gCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGtt < 2:5262349/76‑1 (MQ=255) gCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGtt < 1:798231/76‑1 (MQ=255) gCATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGtt < 1:1286901/76‑1 (MQ=255) cATCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTg > 1:869320/1‑76 (MQ=255) aTCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGt > 1:4710584/1‑76 (MQ=255) aTCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGt < 2:3174690/76‑1 (MQ=255) aTCCCTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGt > 2:5899746/1‑76 (MQ=255) ccTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTAcc < 2:5100897/76‑1 (MQ=255) ccTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTAcc > 1:2618683/1‑76 (MQ=255) cTGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCt > 2:3881534/1‑76 (MQ=255) tGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTg < 1:357216/76‑1 (MQ=255) tGTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTg < 1:4171599/76‑1 (MQ=255) gTCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTgg < 1:5773224/76‑1 (MQ=255) tCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGt < 2:1157238/76‑1 (MQ=255) tCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGt < 1:5149701/76‑1 (MQ=255) tCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGt < 1:478246/76‑1 (MQ=255) tCGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGt < 1:3910893/76‑1 (MQ=255) cGAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTg < 1:1068742/76‑1 (MQ=255) gAACACTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGa < 1:4137585/76‑1 (MQ=255) acgcTTTTGGGCGGCCACGTC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACt < 1:6020765/73‑1 (MQ=255) acacTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACt < 1:3297484/76‑1 (MQ=255) acacTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACt < 2:3667783/76‑1 (MQ=255) acTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACtgt > 1:4414478/1‑76 (MQ=255) cTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACtgtg < 2:1959919/76‑1 (MQ=255) cTTTTGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACtgtg > 2:2139145/1‑76 (MQ=255) ttttGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGtga < 2:1996149/76‑1 (MQ=255) tttGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGtgaa < 1:1078534/76‑1 (MQ=255) ttGGGCGGGCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGtgaat < 2:4714273/76‑1 (MQ=255) ttGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGtgaat > 2:5894410/1‑76 (MQ=255) tGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGtgaatg < 1:1600631/76‑1 (MQ=255) tGGGCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGtgaatg < 1:400444/76‑1 (MQ=255) gggCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGtgaatga < 1:594806/76‑1 (MQ=255) gggCGGCCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGtgaatga < 2:2810388/76‑1 (MQ=255) ggcggcCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGtgaatgaa < 2:3495992/76‑1 (MQ=255) gcggcCACGGTTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGtgaatgaa < 1:1278652/76‑1 (MQ=255) cggcCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAg < 2:192030/76‑1 (MQ=255) cggcCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAg < 1:665441/76‑1 (MQ=255) ggcCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAgg < 2:2476262/76‑1 (MQ=255) ggcCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAgg < 2:5382221/76‑1 (MQ=255) gcCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGt < 1:2933793/76‑1 (MQ=255) gcCACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGt < 1:5535146/76‑1 (MQ=255) ccACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGtt < 1:3621977/76‑1 (MQ=255) ccACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGtt < 1:2831519/76‑1 (MQ=255) ccACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGtt < 1:5039955/76‑1 (MQ=255) ccACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGtt < 1:6174672/76‑1 (MQ=255) ccACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGt > 2:1299705/1‑75 (MQ=255) ccACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGt < 1:1299705/75‑1 (MQ=255) cACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGtta < 1:2968523/76‑1 (MQ=255) cACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGtta < 1:2323808/76‑1 (MQ=255) cACGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGtta < 1:1401680/76‑1 (MQ=255) aCGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGttat < 1:3962456/76‑1 (MQ=255) aCGGC‑TTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGttat < 1:3033598/76‑1 (MQ=255) | AACCGTGTTGCCAGAACAATTTCATGGCCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTAT > REL606/1183318‑1183464 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |