| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,296,053 | (T)5→4 | coding (862/2676 nt) | adhE ← | fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,296,049 | 0 | T | . | 99.3% | 704.1 / ‑3.2 | 143 | coding (866/2676 nt) | adhE | fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base . (63/79); total (63/80) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 172 total aligned reads displayed.
CTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAA > REL606/1295975‑1296120 | cTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGGGGA‑CCAACGATAGCCGCGGTCAGCGCACCCtt > 2:4499621/1‑76 (MQ=255)cTGGAACAAAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCGtt < 2:1251958/76‑1 (MQ=255) tGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCCGGGTGA‑CCAACGATAGCCCCGTTCAGCGCCACGttt > 2:974144/1‑76 (MQ=255) gtaaCAGAAAACCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCGtttt < 1:1035552/73‑1 (MQ=255) ggTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGGTGACCCAACAAAAGCC‑CGCTCCGCGCCCCCtttt > 2:2131940/1‑76 (MQ=255) gTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGGTGA‑CCAACGATAGACCCGGTCAGCGCACCGttttt > 1:1941639/1‑76 (MQ=255) gTAAAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCGttttc < 2:2463655/76‑2 (MQ=255) tACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCGttttca < 2:2650672/76‑3 (MQ=255) aCAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGGTGA‑CCAACGATAGCCGCGTTCAGCGCCCCCgttttag > 1:1832896/1‑74 (MQ=255) agaCGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATa < 1:24497/76‑1 (MQ=255) agaAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGA‑CCAAACATAGACCCGTTCCGCGCACCCACTTTCAgata > 2:713136/1‑73 (MQ=255) atgCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAAc < 2:1755396/74‑1 (MQ=255) gCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACAt < 2:579215/76‑1 (MQ=255) cccTGCCAGTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACAt < 2:3787083/75‑1 (MQ=255) ccTGCCAGTTCAGCAATTTTATAGGCTGGGTGG‑CCAACGAAAGCCCCGGTCCGCGCCCCCTTTTTC‑GGATAACATc > 2:3736718/1‑76 (MQ=255) ccTGCCAGTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATc < 1:5286765/76‑1 (MQ=255) ccTGCCAGTTCAGCAATTTTATAGGCTGGCTGA‑CAAACGAGAGCCGCGTTCAGCGC‑CCCCGTTTCAGGGTAACATc > 1:2949945/1‑76 (MQ=255) cTGCCAGTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATcc < 1:173283/76‑1 (MQ=255) cTGCCAGTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATcc < 2:19303/76‑1 (MQ=255) tGCCAGTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCt < 2:709287/76‑1 (MQ=255) tGCCAGTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCt < 1:360389/76‑1 (MQ=255) tGCCAGTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAAAATCCt > 1:1429136/1‑76 (MQ=255) gCCAGTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTg < 1:1277392/76‑1 (MQ=255) ccAGTTCAGCAATTTTATAGGCTGGGTGA‑CCAACAATAACCCCGGTCAGCGCCCCGCTTTTC‑GGGTAAAATCCtta > 1:4505049/1‑74 (MQ=255) ccAGTTAGGGAATTTTATAGGCTGGCTGA‑CCAGCGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGa < 2:5022955/76‑1 (MQ=255) cAGTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAACCGCGGTCAGCGCCCCG‑CTTTCAGGATAACATCCTGaa > 1:5642133/1‑76 (MQ=255) aGTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAAc < 1:4160532/76‑1 (MQ=255) aGTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAAc < 2:5081024/76‑1 (MQ=255) aGTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAAc < 2:3827783/76‑1 (MQ=255) aGTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAAc < 2:1438933/76‑1 (MQ=255) gTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACa < 2:3359855/76‑1 (MQ=255) gTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACa < 1:2880122/76‑1 (MQ=255) gTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACa < 1:1500168/76‑1 (MQ=255) tCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACaac > 1:4310735/1‑74 (MQ=255) tCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGc < 1:4120693/76‑1 (MQ=255) aGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCtt < 1:4862018/76‑1 (MQ=255) gCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCttt < 2:5627707/76‑1 (MQ=255) cAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTc < 2:3035950/76‑1 (MQ=255) cAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTc < 1:2242270/76‑1 (MQ=255) tttATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGct < 1:3691432/76‑1 (MQ=255) tataGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGctct < 1:3532029/76‑1 (MQ=255) tataGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGctct < 2:1624001/76‑1 (MQ=255) ataGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCtt < 1:6246143/76‑1 (MQ=255) ataGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCtt < 2:4454425/76‑1 (MQ=255) taGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCttt > 1:5378075/1‑76 (MQ=255) taGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCttt < 1:1916236/76‑1 (MQ=255) aGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTa > 2:3414388/1‑76 (MQ=255) aGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTa > 1:3112769/1‑76 (MQ=255) ggctggctGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTAc > 2:6145282/1‑76 (MQ=255) ggctggctGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTAc > 1:64929/1‑76 (MQ=255) ggctggctGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTAc > 1:1371576/1‑76 (MQ=255) ggctggctGA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTAc < 1:4618818/76‑1 (MQ=255) ctggctgA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTAccc > 2:1042839/1‑76 (MQ=255) ctggctgA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTAccc > 1:2714921/1‑76 (MQ=255) ctggctgA‑CCAACGATAGCCGCGTTCAGAGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTAccc > 2:4941725/1‑76 (MQ=255) tggctgA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCt < 1:3446948/76‑1 (MQ=255) tggctgA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCt > 1:4050/1‑76 (MQ=255) ggctgA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTg > 2:5851853/1‑76 (MQ=255) ggctgA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTg < 2:233165/76‑1 (MQ=255) ctgA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCa > 1:366722/1‑76 (MQ=255) ctgA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCa > 1:1567201/1‑76 (MQ=255) ctgA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCa > 1:3410725/1‑76 (MQ=255) gA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAAc < 1:2523031/76‑1 (MQ=255) gA‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAAc > 1:2626268/1‑76 (MQ=255) a‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACa < 2:4407384/76‑1 (MQ=255) a‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACa < 1:2561249/76‑1 (MQ=255) a‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACa > 1:197846/1‑76 (MQ=255) a‑CCAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACa < 2:1276013/76‑1 (MQ=255) ccAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAg > 2:1589177/1‑76 (MQ=255) cAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGa > 2:3355356/1‑76 (MQ=255) cAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGa < 2:3136529/76‑1 (MQ=255) cAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGa > 2:627484/1‑76 (MQ=255) cAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGa > 1:1072314/1‑76 (MQ=255) cAACGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGa > 1:923550/1‑76 (MQ=255) aaCGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGAt > 1:3206430/1‑76 (MQ=255) aaCGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGAt > 2:1903417/1‑76 (MQ=255) aaCGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGAt > 1:4931361/1‑76 (MQ=255) aCGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATa > 2:2265421/1‑76 (MQ=255) aCGATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATa < 1:5966978/76‑1 (MQ=255) gATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAgc < 2:1298267/76‑1 (MQ=255) gATAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAgc > 2:5396468/1‑76 (MQ=255) tAGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAgccg > 1:762173/1‑76 (MQ=255) aGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAgccgc > 1:675525/1‑76 (MQ=255) aGCCGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAgccgc > 1:2028729/1‑76 (MQ=255) ccGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAATAGATAGccgccg > 1:1747757/1‑76 (MQ=255) ccGCGTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGccgccg > 2:1812621/1‑76 (MQ=255) cgcgTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGt > 2:1974082/1‑76 (MQ=255) cgcgTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGt > 2:895324/1‑76 (MQ=255) gcgTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTCCCCTGCAACAGATAGCCGCCGTg < 1:906981/76‑1 (MQ=255) gcgTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTg > 1:5571941/1‑76 (MQ=255) gTTCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTggg > 1:2282798/1‑76 (MQ=255) ttCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGt > 1:2719192/1‑76 (MQ=255) ttCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGt > 1:3848868/1‑76 (MQ=255) tCAGCGCACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGtt > 2:5644966/1‑76 (MQ=255) gcgcACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCa < 1:2131940/76‑1 (MQ=255) gcACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTCCCCTGCAACAGATAGCCGCCGTGGGTTGCaaa < 1:6028511/76‑1 (MQ=255) gcACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCaaa > 2:5988437/1‑76 (MQ=255) cACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCaaaa > 2:2619365/1‑76 (MQ=255) cACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCaaaa < 1:5966655/76‑1 (MQ=255) cACCG‑TTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCaaaa > 2:4150847/1‑76 (MQ=255) | CTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGA‑CCAACGATAGCCGCGTTCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAA > REL606/1295975‑1296120 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |