| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,131,466 | (A)9→8 | intergenic (‑125/+175) | gatY ← / ← fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,131,458 | 0 | A | . | 97.9% | 441.4 / 1.4 | 93 | intergenic (‑117/+183) | gatY/fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base . (75/16); total (75/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.58e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 146 total aligned reads displayed.
AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATATG > REL606/2131383‑2131533 | aaatgaTTTGTTTTTAAGTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa > 1:1784747/1‑76 (MQ=255) aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTTATAGAGGAATGAAAGGCaa < 1:2163733/76‑1 (MQ=255) aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa < 2:5072740/76‑1 (MQ=255) aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa < 1:4740897/76‑1 (MQ=255) aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa < 1:3278072/76‑1 (MQ=255) aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa > 2:5581461/1‑76 (MQ=255) atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa < 2:1201552/76‑1 (MQ=255) atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa > 2:5092383/1‑76 (MQ=255) tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa < 2:352664/76‑1 (MQ=255) tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa < 2:1901024/76‑1 (MQ=255) tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa > 1:2716175/1‑76 (MQ=255) tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa < 1:2083164/76‑1 (MQ=255) gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaa > 2:3110692/1‑76 (MQ=255) gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaa > 1:5792957/1‑76 (MQ=255) gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaa > 2:1905181/1‑76 (MQ=255) aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa > 2:245948/1‑76 (MQ=255) tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaa < 1:4796883/76‑1 (MQ=255) tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaa < 1:4929489/76‑1 (MQ=255) tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac < 2:4229452/76‑2 (MQ=255) tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac < 2:5695078/76‑2 (MQ=255) tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac > 1:3377307/1‑75 (MQ=255) tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac > 2:421246/1‑75 (MQ=255) gTTTTTAAATGTCGAGATAACGATCACGAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacg > 2:2674409/1‑74 (MQ=255) gTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacg > 2:5010337/1‑74 (MQ=255) tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacgg > 2:3679274/1‑73 (MQ=255) tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacgg < 1:4135106/76‑4 (MQ=255) ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 1:200577/1‑76 (MQ=255) ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 1:3457728/1‑76 (MQ=255) ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 2:1593784/1‑76 (MQ=255) ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt < 1:1945989/76‑1 (MQ=255) aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc > 1:5669226/1‑76 (MQ=255) aTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑g < 2:3851802/76‑1 (MQ=255) aTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑g < 2:4526010/76‑1 (MQ=255) gTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGa > 2:4795479/1‑76 (MQ=255) gTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGa > 1:5651433/1‑76 (MQ=255) gTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGa > 1:4720434/1‑76 (MQ=255) tCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAg < 2:3288682/76‑1 (MQ=255) tCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAg > 1:5338808/1‑76 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAGGGC‑AAAAAAAACGGCCTCCC‑GGAgg < 1:830955/76‑1 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAgg > 2:5969378/1‑76 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAgg > 1:749056/1‑76 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑a > 1:4248880/1‑76 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑a < 1:2064978/76‑1 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑a > 1:403819/1‑76 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑a < 1:5739448/76‑1 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑a < 1:746857/76‑1 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑a < 2:2440233/76‑1 (MQ=255) gaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAGACGGCCTCCC‑GGAGGG‑aa > 2:791988/1‑76 (MQ=255) aTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAg > 1:2918861/1‑76 (MQ=255) aTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAg > 2:2194767/1‑76 (MQ=255) aTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAg < 2:2530541/76‑1 (MQ=255) aTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAg > 1:2942713/1‑76 (MQ=255) aTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAg > 2:3568451/1‑76 (MQ=255) tAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGc > 1:1959838/1‑76 (MQ=255) aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGcc < 2:4924991/76‑1 (MQ=255) aCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCC‑GGAGGG‑AAGcc < 1:4843025/76‑1 (MQ=255) aCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCg < 1:3784284/76‑1 (MQ=255) cGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGc > 1:5820120/1‑76 (MQ=255) gATCAAAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGG‑AAAAAAAAACGGCCCCCG‑GGAGGA‑AAGCCGca < 2:2034220/76‑1 (MQ=255) cacaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGcaaca > 1:2132713/1‑76 (MQ=255) cacaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGcaaca > 1:2013720/1‑76 (MQ=255) acaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGcaacaa > 1:3775566/1‑76 (MQ=255) acaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGcaacaa > 2:2035468/1‑76 (MQ=255) caAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACaaa > 2:2867700/1‑76 (MQ=255) aaaaaCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAg > 1:724126/1‑76 (MQ=255) aaCGACAATATGAAAATTAATAGAGGAATGAAAGG‑AAAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTgc < 1:4647873/76‑1 (MQ=255) aaCGACAATATGAAAATTAAAAGAGGAATGAAAGGC‑AAAAAAAACGGCCACCC‑GGAGGA‑AAGCCGCAACAAAGTgc < 1:3142404/76‑1 (MQ=255) aCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTgcg > 1:2902648/1‑76 (MQ=255) cGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTgcgc > 1:3900902/1‑76 (MQ=255) cGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTgcgc > 2:5536409/1‑76 (MQ=255) cGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTgcgc > 2:4664818/1‑76 (MQ=255) aaaTATGAAAATTAAGAGAGGAA‑GAAAAGGAAAAAAAACCGCCCCCCG‑GGAGGA‑AAGCCGCAACAAAGTGCGCGTg < 2:3333387/75‑1 (MQ=255) aaaTATGAAAATAAAGAGAGAAATAAAAGG‑AAAAAAAAACGGCCCCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTg < 1:3480491/75‑1 (MQ=255) atatGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGCGGG‑AAGCCGCAACAAAGTGCGCGTGGt > 1:3816643/1‑76 (MQ=255) atatGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGt > 2:5322279/1‑76 (MQ=255) atGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGttt > 1:4588848/1‑76 (MQ=255) aaaaaaaTAAAAAGAGAAAAGAAAGG‑AAAAAAAACCGCCCCCCG‑GGAGGA‑AAGCCGCAACAAAGTGCGCGTGGttt < 2:954993/73‑1 (MQ=255) tGAAAATAAATGGAGGAAGGAAA‑GAAAAAAAAACCGGCCCCCC‑GGAGGA‑AAGCCGCAACAAAGTGCGCGTGGtttt < 2:3882094/76‑1 (MQ=255) gAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACAGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTa > 1:513213/1‑76 (MQ=255) aaaaTAAATAGAGAAATGAAA‑CCAAAAAAAACCGCCCCCCG‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTAt < 2:4530326/76‑1 (MQ=255) aaaTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATg > 1:5471267/1‑76 (MQ=255) aagtAATAGAGAAATGAAAGAC‑AAAAAAACCGCCCCCCG‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGc < 1:2610732/73‑1 (MQ=255) aaTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGc > 1:5232294/1‑76 (MQ=255) ttAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAg > 2:2879181/1‑76 (MQ=255) tAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg > 2:2998895/1‑76 (MQ=255) aaTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGa > 1:1121557/1‑76 (MQ=255) aTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt > 1:2817602/1‑76 (MQ=255) agagGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGGGCGTGGTTTTATGCAGGATgc > 2:2939254/1‑76 (MQ=255) agagGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc > 2:288153/1‑76 (MQ=255) agagGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTCTATGCAGAATgc > 2:1424735/1‑76 (MQ=255) agaaaTGAAAGG‑‑AAAAAAACCGGCCCCCC‑GGAGGA‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcggc < 2:2882962/73‑1 (MQ=37) gAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑TAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcggcg > 2:5379586/1‑76 (MQ=39) aaaTAAAAGG‑AAAAAAAACCGCCCCCCC‑GGAGGA‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcggcg < 1:4954947/75‑1 (MQ=25) tGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaa > 1:1004245/1‑76 (MQ=255) gAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaaa > 1:4851261/1‑76 (MQ=255) aaaGGC‑AAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 1:1322131/1‑76 (MQ=255) aggaaaaaaaagCGGCCCCCC‑GGAGGA‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACat < 1:1864991/73‑1 (MQ=37) aaaaaaaaaaGCGCCCCCC‑GGAGGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAtata < 1:4757304/75‑1 (MQ=25) aaaaaaaagCGCCCACCG‑GGAGGA‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATAtg < 2:338614/76‑1 (MQ=37) aaaaaaaaaCCGCCCCCCGGGAGGA‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACatat < 2:4847671/76‑1 (MQ=37) | AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCC‑GGAGGG‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATATG > REL606/2131383‑2131533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |