| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 352,377 | (G)8→7 | intergenic (‑139/+47) | frmR ← / ← yaiO | regulator protein that represses frmRAB operon/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 352,370 | 0 | G | . | 98.5% | 636.0 / 1.7 | 130 | intergenic (‑132/+54) | frmR/yaiO | regulator protein that represses frmRAB operon/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base . (56/72); total (57/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
Only 100 of 170 total aligned reads displayed.
GCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAG > REL606/352295‑352441 | gCATCCCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAg < 2:3434447/76‑1 (MQ=255)gCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAg < 2:3821633/76‑1 (MQ=255) cATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgg > 1:1223/1‑76 (MQ=255) cATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgg > 2:3585044/1‑76 (MQ=255) cATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgg > 1:1967229/1‑76 (MQ=255) cATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgg < 2:1958121/76‑1 (MQ=255) cATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgg > 1:5083257/1‑76 (MQ=255) aTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggg < 2:487081/76‑1 (MQ=255) aCCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggg > 1:1037471/1‑76 (MQ=255) aCCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggg > 2:961508/1‑76 (MQ=255) aCCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggg > 2:1629025/1‑76 (MQ=255) aCCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggg < 2:1412894/76‑1 (MQ=255) ccccctTATAGTATATAACACTGGAGAATAAAATTTATCCAGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggg < 2:216790/76‑1 (MQ=255) ccccccTATAGTATATAACACTGGGGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggg > 2:1399047/1‑76 (MQ=255) ccccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggg > 2:5201641/1‑76 (MQ=255) cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggg > 2:2263584/1‑76 (MQ=255) ccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggc > 2:2654473/1‑75 (MQ=255) cccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggtct < 2:692728/76‑4 (MQ=255) cccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggct < 1:2169738/76‑3 (MQ=255) cccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggct < 2:3204954/76‑3 (MQ=255) ccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggcta < 2:5557514/76‑4 (MQ=255) ccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggcta > 1:5433141/1‑73 (MQ=255) ccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggcta > 1:4274722/1‑73 (MQ=255) tataGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAAt > 1:1186060/1‑76 (MQ=255) tataGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAAt > 2:4361439/1‑76 (MQ=255) ataGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATc > 2:4823506/1‑76 (MQ=255) ataGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATc < 1:3135423/76‑1 (MQ=255) taGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCg > 2:3743298/1‑76 (MQ=255) taGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCg < 2:4619440/76‑1 (MQ=255) gTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGc > 2:2987910/1‑76 (MQ=255) gTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGc > 2:5028676/1‑76 (MQ=255) tatataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCa > 1:3276207/1‑76 (MQ=255) tatataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCa > 2:5979437/1‑76 (MQ=255) atataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAg < 2:4832083/76‑1 (MQ=255) tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAgg > 1:3943018/1‑76 (MQ=255) tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAgg < 2:555483/76‑1 (MQ=255) ataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAggg > 1:2100967/1‑76 (MQ=255) ataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAggg < 2:5407673/76‑1 (MQ=255) taACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGa < 1:3561755/76‑1 (MQ=255) taACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGa > 1:2206406/1‑76 (MQ=255) aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGaa < 1:2565644/76‑1 (MQ=255) acacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAg < 2:3400517/76‑1 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGCTAATCGGCAGGGGAg > 2:2399229/1‑76 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGGAgg > 1:5578651/1‑76 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGGAgg > 1:1905273/1‑76 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAgg < 1:4083059/76‑1 (MQ=255) acTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGGAGgc > 1:742182/1‑76 (MQ=255) acTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGgc < 1:1015458/76‑1 (MQ=255) tGGAGAATAAAATTTATCTGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGgccg < 2:1683245/76‑1 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGgccg < 2:5801641/76‑1 (MQ=255) ggAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGgccgc < 2:947404/76‑1 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGGAGgcggc‑c > 1:3517336/1‑76 (MQ=255) aaTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGGCGC‑CCCg > 2:6198804/1‑76 (MQ=255) aaaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGGCGCGCCCggg > 1:578998/1‑75 (MQ=255) aaaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGGCCC‑CCCgggt > 1:2438807/1‑74 (MQ=255) aaaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGGCAC‑CCCCGag > 1:460489/1‑75 (MQ=255) aaaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGAt < 1:2292692/76‑1 (MQ=255) aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATa < 2:6212652/76‑1 (MQ=255) aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATa < 1:2903295/76‑1 (MQ=255) aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATa < 1:1747426/76‑1 (MQ=255) aaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAg < 1:2631325/76‑1 (MQ=255) aTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAg < 2:4373544/39‑1 (MQ=255) ttATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAgcgg > 2:4658777/1‑76 (MQ=255) tATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGGCGC‑CCCCGGGAgcggg > 1:1699954/1‑76 (MQ=255) aTCCGGTGAATGTGGTCGGAAAACAAAGCGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGACAgcgggc > 2:1303497/1‑76 (MQ=255) aTCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAgcgggc < 2:5344054/76‑1 (MQ=255) aTCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAgcgggc < 2:4841257/76‑1 (MQ=255) aTCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAgcgggc < 1:2471096/76‑1 (MQ=255) ccGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGCGCTAATCGGCAGGGAAGGCCGC‑CCCGGCGAgcgggggg > 2:1087833/1‑76 (MQ=255) gTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGGCGC‑CCCGGATAgcggggggggg > 1:5612615/1‑73 (MQ=255) gTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAg < 2:4563387/76‑1 (MQ=255) gAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGaa < 2:2402119/76‑1 (MQ=255) gAATGTGATCGGAAAACAAAGAGGTAA‑GGAGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGaa < 2:1136020/76‑1 (MQ=255) aaTGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGGAGGCCGC‑CCCCGATAGCGGGGGGgaggag > 1:4696128/1‑76 (MQ=255) aaTGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGAAg < 1:6273091/76‑1 (MQ=255) aaTGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCCGGTAGGGGGGGGGCGAAg > 1:6036970/1‑76 (MQ=255) aTGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGAAgg < 2:2230309/76‑1 (MQ=255) aTGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCCGATAGCGGGCGGCAGCAgg > 2:1833094/1‑76 (MQ=255) tgtgGTCGGAAAACAAAGAGGAAAGGGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGAAgg < 2:5691442/76‑1 (MQ=255) tgtgGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGAAGGa < 2:2714036/76‑1 (MQ=255) gtgGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGAAGGaa < 2:4041137/76‑1 (MQ=255) gtgGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGAAGGaa < 2:5991356/76‑1 (MQ=255) tgGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGGAGGCCCC‑CCCCGAAAACCGGCGGCAGCAgggag > 2:988206/1‑73 (MQ=255) tgGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCTGATAACGGGCGGCAGAAGGAAt > 2:6110364/1‑76 (MQ=255) tgGTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGAAGGAAt < 1:1399047/76‑1 (MQ=255) ggTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGAAGGAATc < 2:5171306/76‑1 (MQ=255) ggTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGAAGGAATc < 1:2139358/76‑1 (MQ=255) ggTCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGAGCGGCAGAAGGAATc < 2:4453189/76‑1 (MQ=255) tCGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGAAGGAATCAg < 1:4823506/76‑1 (MQ=255) cGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGAAGGAATCAGa < 2:3268429/76‑1 (MQ=255) cGGAAAACAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGAAGGAATCAGa < 1:4178362/76‑1 (MQ=255) aaaaCAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGGCGC‑CCCCGATAACGGGGGGCCGAAGGAAACaaaaat > 2:5077631/1‑74 (MQ=255) aaaCAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGAAGGAATCAGAAttt < 2:1763577/76‑1 (MQ=255) cAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGGCCC‑CCCCGGTAGCGGGGGGGGGAAGGGAAAAGAAATTCCa > 1:940838/1‑76 (MQ=255) cAAAGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCa < 1:5199966/76‑1 (MQ=255) aaaGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAg < 2:950746/76‑1 (MQ=255) aaGAGGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg < 2:4708237/76‑1 (MQ=255) agagGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGt < 1:2812634/76‑1 (MQ=255) agagGAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGt < 1:1239332/76‑1 (MQ=255) ggAAA‑GGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAg < 1:441814/76‑1 (MQ=255) | GCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGCTAATCGGCAGGGAAGGCCGC‑CCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAG > REL606/352295‑352441 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |