| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,038,538 | (T)9→8 | intergenic (+50/‑167) | bipA → / → yihL | GTP‑binding protein/predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,038,530 | 0 | T | . | 92.1% | 903.8 / 76.8 | 227 | intergenic (+42/‑175) | bipA/yihL | GTP‑binding protein/predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (2/16); new base . (105/104); total (107/120) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01 | |||||||||||
Only 100 of 262 total aligned reads displayed.
TCGTCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAG > REL606/4038455‑4038604 | tcgtcgCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGt < 2:1417864/76‑1 (MQ=255) cgtcgCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtt > 1:5709532/1‑76 (MQ=255) cgtcgCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtt < 2:302838/76‑1 (MQ=255) cgtcgCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtt < 2:3219747/76‑1 (MQ=255) cgtcgCCCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtt < 2:1760731/76‑1 (MQ=255) gtcgCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttt < 2:4323895/76‑1 (MQ=255) tcgCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttt > 2:3423201/1‑76 (MQ=255) tcgCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttt < 2:4478780/76‑1 (MQ=255) tcccgcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGGtttt < 2:2807376/73‑1 (MQ=255) cgcgCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttt > 2:1290753/1‑76 (MQ=255) cgcgCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttt > 2:3344728/1‑76 (MQ=255) cgcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttt < 2:1132926/76‑1 (MQ=255) aaCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttgcc > 2:1015103/1‑73 (MQ=255) ccGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTg < 2:1251120/76‑1 (MQ=255) gcACTGAAAGACGTTTAATTTTGTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGt < 2:2582559/76‑1 (MQ=37) cACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTg < 2:1989829/76‑1 (MQ=39) acggAAAAACGATTATTTTGTTTTTTTAGTAAAAAAAACCCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTg < 1:1615672/73‑1 (MQ=255) aCCGAAAGACGATTAATTTCTTTTTTTAGTAATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGa < 1:4639750/76‑1 (MQ=38) ccGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAg > 2:2380066/1‑76 (MQ=255) cGAAAGACGATTATTTTCGTTTTTTAGTCAAAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAg < 1:2112918/76‑1 (MQ=255) cGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAg < 1:2674370/76‑1 (MQ=255) cGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGc > 1:2475697/1‑76 (MQ=255) cGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGc > 2:5051775/1‑76 (MQ=255) aaGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACt < 2:4699979/76‑1 (MQ=255) gACGTTTATTTTCGTTTTTTAGTCAAAAAAAGCCTGCCGGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCa < 2:5015971/76‑1 (MQ=255) gACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCa > 2:209377/1‑76 (MQ=255) aCGATTAATTTCGTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAg < 2:1052800/76‑1 (MQ=39) ctaTTAATTTCGTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGc < 2:2917899/74‑1 (MQ=255) gtttAATTTCTTTTTTTAGTCAAAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCa < 1:734477/74‑1 (MQ=37) gATTAATTTCGTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCa < 2:5073459/76‑1 (MQ=39) gATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCa < 2:742271/76‑1 (MQ=255) gATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCa < 2:596275/76‑1 (MQ=255) atttAATTTCTTTTTTTGGTAATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCa < 1:4916728/74‑1 (MQ=37) aTTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCaa > 1:440547/1‑76 (MQ=255) ttAATTTCTTTTTTTGGTAATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAAt < 2:1810386/76‑1 (MQ=255) ttAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAAt > 2:2690671/1‑76 (MQ=255) ttAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAAt < 2:2420484/76‑1 (MQ=255) ttAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAAt < 2:6126557/76‑1 (MQ=255) tAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATg > 2:4758097/1‑76 (MQ=255) tAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATg > 2:3119053/1‑76 (MQ=255) tAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATg < 2:3961098/76‑1 (MQ=255) aaTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGc > 2:708597/1‑76 (MQ=255) aaTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGc < 2:6016952/76‑1 (MQ=255) aTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCCATGCg > 1:4430946/1‑76 (MQ=38) aTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCg > 2:1441402/1‑76 (MQ=255) tttCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGa < 2:3745721/76‑1 (MQ=255) tttCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGa > 1:3055554/1‑76 (MQ=255) ttCTTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAg < 2:2429657/76‑1 (MQ=38) ttCGTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAg < 1:3777142/76‑1 (MQ=39) tCGTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAg < 1:2054777/76‑1 (MQ=255) cctttttttAGTCAAAAAAACCCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAg < 1:2423345/73‑1 (MQ=255) cGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTa > 2:4172210/1‑76 (MQ=255) gTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTAt < 2:5892173/76‑1 (MQ=39) gTTTTTTAGTCATAAAAAACCGGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGCGAGCACTCAGCAATGCGCGTAt > 2:3453198/1‑76 (MQ=38) tttaAGTTATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTAtctc < 2:3544187/76‑1 (MQ=38) tttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTcc > 2:2296484/1‑76 (MQ=255) tttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑GTTTTTTTGCCTGGCGTGCGAACTCAGCAATGCGAGTATGTcc > 2:3091665/1‑76 (MQ=37) ttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTccc > 2:6309979/1‑76 (MQ=255) tAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCt < 1:5475086/76‑1 (MQ=39) tAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCt < 2:6185268/76‑1 (MQ=255) tAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGGATCTCCCt > 2:5122528/1‑76 (MQ=38) tCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTtat < 1:1505891/76‑1 (MQ=39) tCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTtat < 1:6310460/76‑1 (MQ=39) tCATAAAAAACCTGCCAGCGATGGCA‑GTTTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTtat < 1:6214138/76‑1 (MQ=255) tCAAAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTtat < 1:3408339/76‑1 (MQ=38) caaaaaaacCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTtata < 1:1208377/73‑1 (MQ=255) cATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTtata < 2:5103992/76‑1 (MQ=255) cATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTtata > 2:1982077/1‑76 (MQ=255) cATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTtata > 1:4302062/1‑76 (MQ=255) aaaaaaaacCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTtata < 1:5371164/73‑1 (MQ=255) aTAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATaa > 2:1901311/1‑76 (MQ=255) aTAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATaa > 2:4089153/1‑76 (MQ=255) aTAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATaa > 1:1802588/1‑76 (MQ=255) tAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAg > 2:4107926/1‑76 (MQ=255) tAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAg > 2:5646671/1‑76 (MQ=255) aacccTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCtg < 1:3926246/73‑1 (MQ=255) aaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGGGTATCTCCCTTATAAGTCtg > 1:1001623/1‑76 (MQ=39) aaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCtg > 2:288548/1‑76 (MQ=255) aaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCtg > 1:4062637/1‑76 (MQ=255) acccTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCtgt < 2:1562142/74‑1 (MQ=255) aaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCtgt > 1:5201011/1‑76 (MQ=255) ccTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCTGTgg < 2:6035299/76‑1 (MQ=255) cTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATTTCCCTTATAAGTCTGTgg < 2:5230186/76‑1 (MQ=255) cTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCTGTGGt > 2:80135/1‑76 (MQ=255) cTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCTGTGGt > 1:820989/1‑76 (MQ=255) tGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCTGTGGtt < 1:2834598/76‑1 (MQ=255) gCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTa < 2:3273786/76‑1 (MQ=255) gCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTa < 2:3774508/76‑1 (MQ=255) gCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTa > 2:4184769/1‑76 (MQ=255) ccAGCGAGGGCA‑GTTTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTAc < 1:4854339/76‑1 (MQ=255) cAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACg < 1:5662445/76‑1 (MQ=255) cGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCa > 2:4403254/1‑76 (MQ=255) cGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCa > 1:5596588/1‑76 (MQ=255) cGATGGCAGG‑GTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCa > 1:5939412/1‑76 (MQ=255) gATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCaa < 2:571234/76‑1 (MQ=255) tGGCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATa < 1:5182868/76‑1 (MQ=255) ggCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATag > 1:3613022/1‑76 (MQ=255) gCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATaga > 1:5440499/1‑76 (MQ=255) gCAGG‑TTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATaga > 2:5484750/1‑76 (MQ=255) gTTTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagagag < 2:4891057/76‑1 (MQ=255) | TCGTCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCACTCAGCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAG > REL606/4038455‑4038604 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |