| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 837,880 | (G)5→6 | coding (226/2226 nt) | ybiO ← | predicted mechanosensitive channel |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 837,875 | 1 | . | G | 98.0% | 395.5 / 0.5 | 98 | coding (231/2226 nt) | ybiO | predicted mechanosensitive channel |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base G (55/41); total (57/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
Only 100 of 122 total aligned reads displayed.
GACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCA‑‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCACAT > REL606/837800‑837945 | gACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTcagca < 1:214837/76‑1 (MQ=255)gACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACAGGTTcagca > 2:5876025/1‑76 (MQ=255)gACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGGACCGGTTcagca > 1:353130/1‑76 (MQ=255)gACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTACTTTTGGTACCGGTTcagca > 2:5056290/1‑76 (MQ=255) aCTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTcagca‑‑g < 1:4852121/76‑1 (MQ=255) aCTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTcagca‑‑g < 1:1439943/76‑1 (MQ=255) aCTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTcagca‑‑g < 2:2432875/76‑1 (MQ=255) ttCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCA‑‑ggg < 2:1845848/76‑1 (MQ=255) ttCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCA‑‑ggg > 1:3206423/1‑76 (MQ=255) tCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCC‑‑gggg > 1:2791974/1‑76 (MQ=255) ggTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCA‑‑gggggg > 2:1571671/1‑75 (MQ=255) ggTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCA‑‑gggggg < 2:2477009/76‑2 (MQ=255) ggTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCA‑‑gggggg < 2:4708010/76‑2 (MQ=255) ggTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACAGGTTCAGCA‑‑gggggg > 2:5979052/1‑75 (MQ=255) ccTGTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAg < 2:1176953/76‑1 (MQ=255) cTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTATTGTACCGGTTCAGCAG‑GGGGGCGTAGc > 2:6162224/1‑76 (MQ=255) ttttGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCg < 1:4377217/76‑1 (MQ=255) tttGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCgg > 1:5735934/1‑76 (MQ=255) ttGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGc > 1:1975374/1‑76 (MQ=255) gcagcaCGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCaa > 2:824152/1‑76 (MQ=255) cagcaCGATTTGCTCTTCGACCAGCGTCGGCGGCAATATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAAc > 2:1804794/1‑76 (MQ=255) gcaCGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAAccg > 2:4288764/1‑74 (MQ=255) caCGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGt < 2:1107661/76‑1 (MQ=255) aCGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCCACGGTg > 2:5813507/1‑76 (MQ=255) aCGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACgggg > 1:2856103/1‑74 (MQ=255) ctgTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTgc > 1:6060846/3‑76 (MQ=255) cGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTgc < 2:4171265/76‑1 (MQ=255) ggTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGGGCGTAGCGGCAACGGGgc > 2:4437228/1‑76 (MQ=255) ggTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTgcg > 2:1682215/1‑76 (MQ=255) ggTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGGgcg > 2:1998438/1‑76 (MQ=255) ggTTTGCGCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTgca < 2:6110164/76‑2 (MQ=255) gTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTgcgc > 1:3603087/1‑76 (MQ=255) gTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCCGCAAAGGTgcgc > 2:5792071/1‑76 (MQ=255) ttGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGGGCGCaa > 1:4618524/1‑76 (MQ=255) ttGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAAAGGTGCGCaa > 2:721576/1‑76 (MQ=255) tGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAAc > 2:6046170/1‑76 (MQ=255) tGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAAACGTGCGCCAc > 1:1147064/1‑76 (MQ=255) gCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACt < 1:121911/76‑1 (MQ=255) gCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACt > 1:940355/1‑76 (MQ=255) gCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCA‑‑GGGGGCGTAGCGGCAACGGTGCGCAACTg > 1:4919590/1‑76 (MQ=255) ctTCGGCCAGCGTCGGCGGTACTATTTTTGGTACCGGTTAAGCAG‑GGGGGCGTAGCCGCAACAGGGCGCAACTGGt > 2:341247/1‑76 (MQ=255) ctTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGGGCGCAACTggc > 2:2977978/1‑75 (MQ=255) ttCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTc > 1:6147315/1‑76 (MQ=255) ttCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTc < 1:5884520/76‑1 (MQ=255) tCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCg > 2:1487727/1‑76 (MQ=255) cGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGa < 1:900554/76‑1 (MQ=255) gACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGAt < 1:5961818/76‑1 (MQ=255) aCCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGCTc > 1:3555847/1‑76 (MQ=255) ccAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCa < 1:3822869/76‑1 (MQ=255) ccAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCa < 1:136506/76‑1 (MQ=255) ccAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCa < 2:3159228/76‑1 (MQ=255) ccAGCGTCGGCGGCACTATGTTTGGTACCGATTCAGCAG‑GGGGGCTTAGCGGCAACGGTGCGCAACTGGTCGATCa < 2:307813/76‑1 (MQ=255) cAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCaa < 2:4948947/76‑1 (MQ=255) cAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGGGCGCAACTGGTCGATCaa > 2:2004017/1‑76 (MQ=255) aGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGGGCGCCACTGGTCGATCAAc > 1:778020/1‑76 (MQ=255) aGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGGGCGCAACTGGGCGATCAAc > 2:5991353/1‑76 (MQ=255) gCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAAct < 1:824152/76‑1 (MQ=255) gTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAActct < 1:1737525/76‑1 (MQ=255) gTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAActct < 1:480213/76‑1 (MQ=255) gTCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGGCGATCAActct > 1:3944430/1‑76 (MQ=255) tCGGCGGCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCtt < 2:2592257/76‑1 (MQ=255) cggcggGACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGTGGCAACGGTGCGAAACTGGTCGATCAACTCttt < 2:4552995/76‑1 (MQ=255) cggcggCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCttt > 2:1340345/1‑76 (MQ=255) cggcggCACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCttt < 1:5442666/76‑1 (MQ=255) gcggcACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTAc < 1:5429046/76‑1 (MQ=255) cggcACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTAcg > 1:3319770/1‑76 (MQ=255) gcccTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTAcgcg < 1:2452937/73‑1 (MQ=255) gcACTATTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTAcgcg < 1:1117861/76‑1 (MQ=255) aTTTTTGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGgtg > 2:3833486/1‑76 (MQ=255) ttttGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTc > 1:3995814/1‑76 (MQ=255) tttGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGTTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCa > 1:4628474/1‑76 (MQ=255) tttGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCa > 1:2614032/1‑76 (MQ=255) ttGGTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCAt > 2:5593638/1‑76 (MQ=255) ggTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTa > 2:2783431/1‑76 (MQ=255) ggTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTa > 1:5237959/1‑76 (MQ=255) ggTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTa < 1:1163379/76‑1 (MQ=255) gTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTAt < 1:5417227/76‑1 (MQ=255) gTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTAt < 1:5249673/76‑1 (MQ=255) gTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTAt > 1:3097448/1‑76 (MQ=255) gTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTAt > 2:1140155/1‑76 (MQ=255) gTACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGGGTCATTAt > 2:5816001/1‑76 (MQ=255) tACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCCTTATc > 1:5538514/1‑76 (MQ=255) tACCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATc > 1:504187/1‑76 (MQ=255) tACCGGTTCAGCAG‑GGGGGCGAAGCGGCAACGGAGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATc > 2:4718902/1‑76 (MQ=255) aCCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATcc < 1:2344652/76‑1 (MQ=255) aCCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATcc < 1:1998438/76‑1 (MQ=255) aCCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATcc < 2:3860806/76‑1 (MQ=255) aCCGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATcc > 2:513198/1‑76 (MQ=255) ccGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCa < 1:4134981/76‑1 (MQ=255) cGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAg < 1:3978389/76‑1 (MQ=255) cGGTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAg > 2:2276208/1‑76 (MQ=255) ggTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGc < 1:2394313/76‑1 (MQ=255) gTTCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCAAGca > 2:4576630/1‑76 (MQ=255) ttCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGcac < 2:5953237/76‑1 (MQ=255) ttCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGcac > 2:5894949/1‑76 (MQ=255) ttCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGcac < 1:299675/76‑1 (MQ=255) ttCAGCAG‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGcac > 2:1327046/1‑76 (MQ=255) cagcag‑GGGGGCGTAGCGGCAACGGTGCGCCACTGGTCGAACAACCTTTTACGCGCGGTGGCATTATACAGAACAt > 2:343867/1‑76 (MQ=255) cagcag‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCACAt < 2:205614/76‑1 (MQ=255) cagcag‑GGGGGCGTAGCAGCACCGGTGCGCAACTGGGCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCACAt < 2:5649523/76‑1 (MQ=255) | GACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCA‑‑GGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCACAT > REL606/837800‑837945 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |