| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,111,966 | (G)7→8 | intergenic (‑235/+167) | insE‑1 ← / ← serX | putative transposase‑related protein/tRNA‑Ser |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,111,959 | 1 | . | G | 97.1% | 133.8 / ‑3.0 | 35 | intergenic (‑228/+174) | insE‑1/serX | putative transposase‑related protein/tRNA‑Ser |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (0/34); total (0/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAA > REL606/1111885‑1112025 | aTCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑g < 2:2651229/76‑1 (MQ=255)aTCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑g < 2:4923373/76‑1 (MQ=255)aTCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑g < 1:4275125/76‑1 (MQ=255)aTCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑g < 2:3630741/76‑1 (MQ=255)aTCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑g < 2:1026092/76‑1 (MQ=255) tCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑gg < 1:4538726/76‑1 (MQ=255) tCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑gg < 1:4078245/76‑1 (MQ=255) tCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑gg < 2:3263075/76‑1 (MQ=255) ccccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑gg‑g > 1:2886423/1‑76 (MQ=255) ccccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGGGA‑gg‑g > 2:6050340/1‑76 (MQ=255) cccccGGGTTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATAT‑GC‑GTTGA‑gg‑g‑gg < 2:5249587/76‑1 (MQ=14) cccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑gg‑g‑ggg < 1:3004755/76‑1 (MQ=255) ccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑gg‑g‑ggg‑g < 2:2920566/76‑1 (MQ=255) cGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGg‑gg‑g‑ggg‑gg > 2:1094050/1‑75 (MQ=255) cGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑gg‑g‑ggg‑gg < 2:989926/76‑2 (MQ=255) ggTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑gg‑g‑gag‑gga < 1:3645484/76‑3 (MQ=255) ggTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGGGA‑gg‑g‑ggg‑ggg > 1:2560648/1‑74 (MQ=255) gtgtgtGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGGGT‑gg‑g‑ggg‑ggcg > 1:2660840/1‑73 (MQ=14) cAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACtcg < 2:3735561/76‑1 (MQ=255) atatACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACtcgct < 1:5356601/76‑1 (MQ=255) tataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACtcgctt < 1:1184446/76‑1 (MQ=255) taCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACtcgcttcg < 1:4150706/76‑1 (MQ=255) aaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGccca < 2:4763269/76‑2 (MQ=255) aaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCt < 1:4053595/76‑1 (MQ=255) aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTg < 2:4323798/76‑1 (MQ=255) aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTg < 2:3804040/76‑1 (MQ=255) aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTg < 2:1988062/76‑1 (MQ=255) aaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GGGTGAATCGCTTCGCccccccctc > 1:4505050/1‑73 (MQ=14) aGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCg < 2:3759515/76‑1 (MQ=255) gCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGGG‑GG‑G‑GGG‑GGGTGACTCCCTTCCCTCCCCCCGccg > 2:6258318/1‑74 (MQ=9) cccGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCggg < 1:3958091/76‑1 (MQ=255) cGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCa < 2:988304/76‑1 (MQ=255) cGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCa < 1:1905939/76‑1 (MQ=255) cGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCa < 1:881981/76‑1 (MQ=255) gTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAg < 2:5004222/76‑1 (MQ=255) gTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAg < 2:4497730/76‑1 (MQ=255) gTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAg < 2:2883827/76‑1 (MQ=255) gTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAg < 2:3766453/76‑1 (MQ=255) tttCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCg < 2:5392910/76‑1 (MQ=9) tCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCt < 1:5986046/76‑1 (MQ=255) tCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCt < 2:431894/76‑1 (MQ=255) tACGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCAc < 1:3579330/76‑1 (MQ=255) cGAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTg < 2:1483641/76‑1 (MQ=255) gAGCTCTTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGc < 2:5234303/76‑1 (MQ=255) ctctTCTTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGtt < 2:58295/76‑1 (MQ=255) tcttctTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTc < 1:824248/76‑1 (MQ=255) tcttctTTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTc < 1:2148659/76‑1 (MQ=255) tcttctTTAAATATGGC‑GGTGA‑GG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCa < 1:3100383/76‑1 (MQ=35) cttcttTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCa < 1:4318196/76‑1 (MQ=255) tcttTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACg < 1:4152219/76‑1 (MQ=255) tcttTAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACg < 1:4889492/76‑1 (MQ=255) tcttTAAACACGGC‑GGCGG‑GG‑G‑GGGAGCTTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACg < 2:1964623/76‑1 (MQ=14) tAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCt < 2:5328111/76‑1 (MQ=255) tAAATATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCt < 2:584086/76‑1 (MQ=255) aaaTATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTg < 2:4391840/76‑1 (MQ=255) aaaTATGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTg < 1:6258318/76‑1 (MQ=255) actatGGC‑GGTGA‑GGTA‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGt < 2:3024169/74‑1 (MQ=14) catGGC‑GGTGA‑GG‑G‑GGGAGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTcc < 2:269816/75‑1 (MQ=255) atGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCa < 1:5487089/76‑1 (MQ=255) atGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCa < 2:2277229/76‑1 (MQ=255) atGGC‑GGCTA‑GG‑GAGGG‑TATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCa < 2:164344/76‑1 (MQ=11) tGGC‑GGTGAGGG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCaa < 1:3790309/76‑1 (MQ=255) | ATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GG‑G‑GGG‑GATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAA > REL606/1111885‑1112025 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |