| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,405,401 | (G)6→7 | coding (438/1347 nt) | fadL → | long‑chain fatty acid outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,405,395 | 1 | . | G | 99.4% | 658.5 / ‑4.4 | 160 | V144V (GTC→GTG) | fadL | long‑chain fatty acid outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base . (1/0); major base G (67/91); minor base A (1/0); total (69/91) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
Only 100 of 193 total aligned reads displayed.
TGGTTGGGGCGCTTCTATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑C‑‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGA > REL606/2405320‑2405465 | tggttggGGCGCTTCTATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑c > 2:3867657/1‑76 (MQ=255) ggttggGGCGCTTCTATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑C‑‑g > 1:2023603/1‑76 (MQ=255) ttggGGCGCTTCTATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑C‑‑ggg < 1:4256131/76‑1 (MQ=255) ttggGGCGCTTCTATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑C‑‑ggg < 1:2722315/76‑1 (MQ=255) ggggCGCTTCTATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑C‑‑ggggg < 1:5136025/76‑1 (MQ=255) ggCGCTTCTATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑C‑‑ggggggg < 2:1462947/76‑2 (MQ=255) ggCGCTTCTATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑C‑‑ggggggg < 2:2657924/76‑2 (MQ=255) cgcTTCTATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑C‑‑gggggggac < 1:749100/76‑4 (MQ=255) ttCTATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAAc < 2:1631091/76‑1 (MQ=255) tATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGGCAACCGa > 2:2577158/1‑76 (MQ=255) aTTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGAc > 1:6268451/1‑76 (MQ=255) aTTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑C‑‑GGGGGGGAAACCGAcc > 1:5383159/1‑76 (MQ=255) ttACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGAcc > 1:3656702/1‑76 (MQ=255) aCCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCtt < 2:4880054/76‑1 (MQ=255) aCCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCCACCtt > 2:2015694/1‑76 (MQ=255) aCCACTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCtt < 1:241461/76‑1 (MQ=255) ctctAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGTCC‑‑GGGGGGACCACCGCCCCTG‑a > 2:3016587/1‑76 (MQ=255) ctctAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑a < 2:1706765/76‑1 (MQ=255) tAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAAc > 2:2251975/1‑76 (MQ=255) tAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAAc > 1:5722135/1‑76 (MQ=255) aaCTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGGCAACCGACCTTG‑AAAcc > 1:1700887/1‑76 (MQ=255) aaCTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGGCAACCGACCTTG‑AAAcc > 1:3255241/1‑76 (MQ=255) aaCTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAAcc > 1:2798868/1‑76 (MQ=255) aCTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCa < 1:4217529/76‑1 (MQ=255) cTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGGCAACCGACCTTG‑AAACCAt > 1:5045201/1‑76 (MQ=255) cTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCAt < 1:1539354/76‑1 (MQ=255) cTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCCTG‑AAAACAt > 2:3440593/1‑76 (MQ=255) tATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGGCAACCGACCTTG‑AAACCATg > 1:293391/1‑76 (MQ=255) tATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGGCAAACGACCTTG‑AAACCATg > 2:466407/1‑76 (MQ=255) tATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATg > 1:2637580/1‑76 (MQ=255) tATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATg > 1:2379440/1‑76 (MQ=255) aTGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGa < 2:4395037/76‑1 (MQ=255) aTGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGa < 1:5808444/76‑1 (MQ=255) aTGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGa > 1:1865541/1‑76 (MQ=255) aTGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGa < 1:2906989/76‑1 (MQ=255) tGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGaa < 2:1068067/76‑1 (MQ=255) ggTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGGCAATCCACCTTGCAAACCATTaa > 2:2693189/1‑76 (MQ=255) ggTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAAc < 1:4029003/76‑1 (MQ=255) gTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CGGGGGGGGACAACCTACCTTG‑AAACCATGAAc > 1:5022053/1‑76 (MQ=255) gTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGTCCTTG‑AAACCCTGAAcc > 1:1848510/1‑76 (MQ=255) tCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCt < 2:3681227/76‑1 (MQ=255) tCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCt < 1:6015812/76‑1 (MQ=255) cTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTg < 2:4456622/76‑1 (MQ=255) tGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGa < 1:2509079/76‑1 (MQ=255) tGGCTACAGAGTGTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGa < 2:1238171/76‑1 (MQ=255) ggCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGaa < 2:3637289/76‑1 (MQ=255) ggCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGaa < 2:4147156/76‑1 (MQ=255) gCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAAc < 1:3686699/76‑1 (MQ=255) cTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGGCAACCGAACTTG‑AAACCATGAACCTGAACt > 2:1699039/1‑76 (MQ=255) tACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACtt < 1:6290953/76‑1 (MQ=255) cAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTaa < 2:1474672/76‑1 (MQ=255) cAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTaa < 1:4965815/76‑1 (MQ=255) cAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTaa < 2:3077936/76‑1 (MQ=255) agagTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAg > 2:399520/1‑76 (MQ=255) agTTTAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCg > 1:2479862/1‑76 (MQ=255) tAACGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGc < 1:549735/76‑1 (MQ=255) aaCGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCg < 2:1286378/76‑1 (MQ=255) aCGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGt > 1:1394022/1‑76 (MQ=255) aCGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGt > 2:5805662/1‑76 (MQ=255) cGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTa > 1:1213427/1‑76 (MQ=255) cGATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTa > 1:1140480/1‑76 (MQ=255) cGATACTTATGCAGGAGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTa < 1:2989622/76‑1 (MQ=255) gATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCCTGAACCTGAACTTAAGCGGTGCGTAt > 2:4811507/1‑76 (MQ=255) gATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTAt < 1:2633487/76‑1 (MQ=255) gATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTAt < 2:3136728/76‑1 (MQ=255) gATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTAt < 2:5827366/76‑1 (MQ=255) gATACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTAt < 1:4602303/76‑1 (MQ=255) aTACTTATGCAGGCGGCTCTGT‑CGGGGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTAt < 1:5299905/76‑1 (MQ=255) aTACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATc > 1:485982/1‑76 (MQ=255) aTACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAAACCACACTG‑AAACCACGAACCCGAAATCAAGCGGTGCGTGTc > 2:3667603/1‑76 (MQ=255) tACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCg < 1:960669/76‑1 (MQ=255) tACTTATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCg < 2:1366824/76‑1 (MQ=255) ttATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCtt < 2:2443539/76‑1 (MQ=255) ttATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCtt < 2:5114414/76‑1 (MQ=255) tATGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTa < 2:734436/76‑1 (MQ=255) aTGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTaa < 1:2302053/76‑1 (MQ=255) aTGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTaa < 1:2513973/76‑1 (MQ=255) tGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTaaa < 1:4002457/76‑1 (MQ=255) tGCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTaaa > 1:2575211/1‑76 (MQ=255) gCAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaat < 1:4331316/76‑1 (MQ=255) cAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaata > 2:6263536/1‑76 (MQ=255) cAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaata < 2:775915/76‑1 (MQ=255) cAGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaata > 2:1129585/1‑76 (MQ=255) aGGCGGCTCTGT‑CGGGGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaata < 2:5246981/76‑1 (MQ=255) aGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaataa < 2:4605781/76‑1 (MQ=255) aGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaataa > 2:47392/1‑76 (MQ=255) aGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaataa < 1:2162690/76‑1 (MQ=255) aGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaataa > 2:4482683/1‑76 (MQ=255) aGGCGGCTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaataa > 1:4116805/1‑76 (MQ=255) ggcggcTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaataat < 1:3463589/76‑1 (MQ=255) ggcggcTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAaataat < 2:6077604/76‑1 (MQ=255) ggcTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCa < 2:1928129/76‑1 (MQ=255) ggcTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCa > 1:6143408/1‑76 (MQ=255) ggcTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCa < 1:4472912/76‑1 (MQ=255) gcTCTGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCAt < 2:557707/76‑1 (MQ=255) tctGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATgg < 1:874686/76‑1 (MQ=255) tctGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATgg < 2:887294/76‑1 (MQ=255) ctGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGa < 2:3485018/76‑1 (MQ=255) ctGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGa > 2:6299475/1‑76 (MQ=255) ctGT‑CG‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGa > 1:2961666/1‑76 (MQ=255) | TGGTTGGGGCGCTTCTATTACCTCTAACTATGGTCTGGCTACAGAGTTTAACGATACTTATGCAGGCGGCTCTGT‑C‑‑GGGGGGACAACCGACCTTG‑AAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGA > REL606/2405320‑2405465 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |