| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,402,611 | (A)7→8 | intergenic (+70/+59) | sgaE → / ← yjfY | L‑ribulose 5‑phosphate 4‑epimerase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,402,604 | 1 | . | A | 99.4% | 666.7 / ‑3.3 | 162 | intergenic (+63/+66) | sgaE/yjfY | L‑ribulose 5‑phosphate 4‑epimerase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (79/82); total (80/82) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01 | |||||||||||
Only 100 of 190 total aligned reads displayed.
CGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTTATACCTGGTGCTAGC > REL606/4402529‑4402674 | cGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTc > 1:3610811/1‑76 (MQ=255)cGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTc > 2:2242007/1‑76 (MQ=255) gggCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑a > 1:3399517/1‑76 (MQ=255) gCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑aaa < 2:1857565/76‑1 (MQ=255) gCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑aaa > 1:4167629/1‑76 (MQ=255) cAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑aaaa > 2:3113122/1‑76 (MQ=255) aGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑aaaaa > 2:6300331/1‑76 (MQ=255) aGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑aaaaa > 2:253148/1‑76 (MQ=255) aGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑aaaaa > 1:374323/1‑76 (MQ=255) aGAAGTAGAACAAGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑aaaaa > 2:3966296/1‑76 (MQ=255) gAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑aaaaaa > 2:6240833/1‑76 (MQ=255) gTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑aaaaaaaag > 2:3822379/1‑74 (MQ=255) acacGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGccccc > 1:6255679/1‑76 (MQ=255) tcgcgCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTg < 2:4824659/75‑1 (MQ=255) acGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTg < 1:3059558/76‑1 (MQ=255) gcgcTGCGTAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCCCCCGTTTTTCAAAAAAAAGCCCCCTgag < 1:6305739/76‑1 (MQ=255) gcgcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTgag < 1:218378/76‑1 (MQ=255) gcgcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTgag < 2:2691490/76‑1 (MQ=255) ggcTGCGGAAATTTCCTTCCTCGGGATATAGCTGGCCTGATTCCGCCTCCGTTTTTCAAAAAAAAGCCCCCTgaga < 2:270441/75‑1 (MQ=255) cgcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTgaga < 1:92263/76‑1 (MQ=255) gcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGaa > 2:3601261/1‑76 (MQ=255) gcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGaa > 1:219695/1‑76 (MQ=255) cTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAg > 1:2502612/1‑76 (MQ=255) cTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAg > 2:2924579/1‑76 (MQ=255) tGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAgg < 1:4384853/76‑1 (MQ=255) gCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAggg > 2:3019485/1‑76 (MQ=255) cGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAgggg < 1:246903/76‑1 (MQ=255) cGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAgggg < 1:1973326/76‑1 (MQ=255) aattttCCTTCCTCGGGAGAAAACTGGTCTAATCCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGgca < 1:2100561/73‑1 (MQ=255) aaaTTTCCTTCCTCGGGAGATACCTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGgca < 2:1063687/76‑1 (MQ=255) aaTTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGgcaa > 2:2630861/1‑76 (MQ=255) tttCCTTCCTGGGGAGATAACGGGTCTAATTCCGCCCCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGgcaaag < 2:1748842/76‑1 (MQ=255) tttCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGgcaaag < 2:6109378/76‑1 (MQ=255) ttccttccTCGGGAGATAACTGGTCTAATTCCGCATCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGgcaaagc < 2:3775322/76‑1 (MQ=255) ttccttccTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGgcacagc > 2:5652555/1‑76 (MQ=255) ttccttccTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGgcaaagc > 1:5152643/1‑76 (MQ=255) ttccttccTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGgcaaagc > 1:387361/1‑76 (MQ=255) tccttcctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGgcaaagca < 2:2167812/76‑1 (MQ=255) ccttcctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGgcaaagc < 1:5486149/74‑1 (MQ=255) cttcctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGgcaaagcaaa > 1:1209428/1‑76 (MQ=255) cttcctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGgcaaagcaaa < 2:4610588/76‑1 (MQ=255) cttccgCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGgcaaagcaaa < 2:4170838/76‑1 (MQ=255) ttcctCGGGAGATAACTGGTCTAATTCCGCTTCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAAc < 2:3621720/76‑1 (MQ=255) ttcctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAAc > 1:5442175/1‑76 (MQ=255) ttcctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAAc > 2:6046200/1‑76 (MQ=255) tcctCGGGAGATAACTGGACTAATTCGCCTTCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACt < 1:3553103/76‑1 (MQ=255) cctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTa < 2:4535848/76‑1 (MQ=255) ctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTAt < 2:4339570/76‑1 (MQ=255) ctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTAt < 2:1441879/76‑1 (MQ=255) ctCGGAAAATAGCTGGTCTATTTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTAt < 2:4258067/76‑1 (MQ=255) cGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATgg > 2:3942779/1‑76 (MQ=255) gggAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGc > 2:5889191/1‑76 (MQ=255) gggAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGc < 1:2836946/76‑1 (MQ=255) ggAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCa < 2:2364207/76‑1 (MQ=255) agaTAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAAt > 2:1373531/1‑76 (MQ=255) agaTAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAAt < 2:3704809/76‑1 (MQ=255) agaTAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAAt > 2:1600810/1‑76 (MQ=255) aTAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGt > 2:2098483/1‑76 (MQ=255) aTAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGt > 2:5014187/1‑76 (MQ=255) aTAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGt > 1:6119033/1‑76 (MQ=255) tAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGtt < 1:1272004/76‑1 (MQ=255) tAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGtt > 1:4245191/1‑76 (MQ=255) aaCTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGttt < 1:4109602/76‑1 (MQ=255) aaCTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGttt < 2:1318003/76‑1 (MQ=255) cTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCt > 1:4855844/1‑76 (MQ=255) atGGTCTAATTCCCCATCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCt < 2:3290149/75‑1 (MQ=255) tGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCtt > 1:6051134/1‑76 (MQ=255) tGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCtt < 1:1574484/76‑1 (MQ=255) ggTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGGAGGGGGCAAAGCAAACTATTGCCATGTTTCttt > 2:4302947/1‑76 (MQ=255) ggTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCttt < 1:847844/76‑1 (MQ=255) tCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTgg > 1:4575395/1‑76 (MQ=255) cTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGt > 2:5459341/1‑76 (MQ=255) cTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGt > 1:2464985/1‑76 (MQ=255) cTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGt > 1:5886308/1‑76 (MQ=255) tAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGtt > 1:205676/1‑76 (MQ=255) tAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGtt > 1:1009901/1‑76 (MQ=255) aaTTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTta > 1:4901929/1‑76 (MQ=255) aTTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTtat > 1:2566158/1‑76 (MQ=255) ttCCGCTGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTtata < 1:5270451/76‑1 (MQ=255) ttCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTtata < 2:2786165/76‑1 (MQ=255) tCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTTATAc > 2:1123753/1‑76 (MQ=255) ccGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTTATAcc > 2:3732073/1‑76 (MQ=255) ccGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTTATAcc > 2:4050029/1‑76 (MQ=255) cGCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTTATACCt < 1:5032464/76‑1 (MQ=255) gCAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTTATACCTg < 2:350892/76‑1 (MQ=255) cAGCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTTATACCTgg < 1:4291005/76‑1 (MQ=255) cgCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTTATACCTGGt < 1:4617357/75‑1 (MQ=255) tccGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTTATACCTGGTg < 2:4862750/75‑1 (MQ=255) gCCGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTTATACCTGGTg < 2:6177683/76‑1 (MQ=255) ccGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTTATACCTGGTGc > 2:809899/1‑76 (MQ=255) cGTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTTATACCTGGTGCt < 1:3113122/76‑1 (MQ=255) gTTTTTCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTTATACCTGGTGCTa > 2:5787870/1‑76 (MQ=255) tttttCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTTATACCTGGTGCTAg > 2:5526761/1‑76 (MQ=255) tttttCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTTATACCTGGTGCTAg > 2:2733772/1‑76 (MQ=255) tttttCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTTATACCTGGTGCTAg > 2:5216792/1‑76 (MQ=255) tttttCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTTATACCTGGTGCTAg < 1:3747117/76‑1 (MQ=255) cttttCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTTATACCTGGTGCTAg > 1:4888769/2‑76 (MQ=255) ttttCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTTATACCTGGTGCTAGc < 2:2310903/76‑1 (MQ=255) ttttCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTTATACCTGGTGCTAGc > 1:1608295/1‑76 (MQ=255) ttttCAAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTTATACCTGGTGCTAGc > 2:1016725/1‑76 (MQ=255) | CGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCTTTGGTTATACCTGGTGCTAGC > REL606/4402529‑4402674 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |