| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,853,067 | 0 | A | C | 35.2% | 156.0 / 75.6 | 105 | T56P (ACC→CCC) | yeaN | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base A (58/10); new base C (0/37); total (58/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.63e-19 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 130 total aligned reads displayed.
ACCGGCGCAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGAACG > REL606/1852992‑1853140 | aCCGGCGCAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGacca < 2:1724209/76‑1 (MQ=255) ccGGCGCAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGaccac < 2:3065447/76‑1 (MQ=255) cGGCGCAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAAACGGCTTATTTaccacc > 2:3157759/1‑76 (MQ=255) gcgcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCt < 2:2821271/76‑1 (MQ=255) gcgcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCt < 1:5091298/76‑1 (MQ=255) gcgcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCt < 1:5687222/76‑1 (MQ=255) gcgcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCt < 2:3844234/76‑1 (MQ=255) cgcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGGCGACAGAGCAAACCCGCTTATTTACCAccccg > 2:2391128/1‑74 (MQ=255) cgcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTTACCACCCTg > 1:2134746/1‑76 (MQ=255) cgcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCtc > 1:3817555/1‑75 (MQ=255) cgcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTg < 2:5322524/76‑1 (MQ=255) gcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGAACACCCCGc > 2:1912922/1‑76 (MQ=255) cAGCGCCTTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGcc < 2:5637746/76‑1 (MQ=255) cAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGcc < 2:801320/76‑1 (MQ=255) cctttACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCCCCCTGCCattat < 1:1272413/73‑1 (MQ=255) cGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCactatt > 2:42357/1‑76 (MQ=255) gTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTg > 1:218529/1‑76 (MQ=255) ttACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTTACCACCCTGCCattatttg > 2:4911005/1‑74 (MQ=255) ttACGGGATACGTTTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCCCCCTGCCATTATTgg < 1:3131927/76‑1 (MQ=255) tACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGc > 1:3783732/1‑76 (MQ=255) tACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAAACGGCTTATTGACCCACCTGCCATTATTGGc > 2:5979535/1‑76 (MQ=255) tACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAAACGGCTTATTGACCACCCTGCCCTTATTGGc > 2:4684819/1‑76 (MQ=255) aCTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCCGCCCTTATTgggc > 1:5946550/1‑74 (MQ=255) cTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCt > 1:6079777/1‑76 (MQ=255) cTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGAGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCt > 2:3591401/1‑76 (MQ=255) tgaaTACGTTGCGTCCCCCTTCCCCGCTGACGACAGCGCAACCCGGCTTATTGCCCCCCCTGCCATTATTGGCCtt < 2:1928076/73‑1 (MQ=255) tGGATACGATTCGTTCCGCTTCCCCGCTGACGACAGCGCAACCCGGCTTATTGCCCCCCCTGCCATTATTGGCCtt < 1:1770906/76‑1 (MQ=255) tGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCCTTATTGGCCtt > 1:6319543/1‑76 (MQ=255) tGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCtt > 2:1914812/1‑76 (MQ=255) tGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCtt > 2:4436186/1‑76 (MQ=255) tGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCtt > 1:2824127/1‑76 (MQ=255) tGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCtt > 2:519592/1‑76 (MQ=255) tGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCAACCTGCCATTATTGGCCtt > 2:5047205/1‑76 (MQ=255) gggATACGATTCGTCCCTCTTCCCCGCTGACGACAGCGCAACCCGGCTTATTGCCCCCCCTGCCATTATTGGCCtt < 2:2375237/75‑1 (MQ=255) ggATACGATTCGTTCCGCTTCCCCGCGGACGCCCGCGAAACCCGGTTTATTGCCCCCCCTGCCATTATTGGCCttt < 2:1851202/76‑1 (MQ=255) ggATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCttt > 2:1612664/1‑76 (MQ=255) ggATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCttt > 1:268970/1‑76 (MQ=255) ggATACGATTCGTTCCCCTTCCTCGCTGACGACAGCGCAACCCGGCTTATTGCCCCCCCTGCCATTATTGGCCttt < 2:3970189/76‑1 (MQ=255) gATACGTTTCGTCCCGCTTCCCCGCTGACGACAGCGCAACCCGGCTTATTGACCCCCCTGCCATTATTGGCCTTTg < 1:540642/76‑1 (MQ=255) gATACGATTGGGCCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTg < 2:1313079/76‑1 (MQ=255) gATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCCCCCTGCCATTATTGGCCTTTg < 2:1404167/76‑1 (MQ=255) aTACGATTCGTCCCGTTTCCCCGCTGACGACAGCGCAACCCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTgc < 1:3262320/76‑1 (MQ=255) aCGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTgcgc > 2:2974080/1‑76 (MQ=255) cgtttCGTTCCCCTTCCTCGCTGACGACAGCGCAACCCGGCTTTTTGCCCCCCCTGCCATTATTGGCCTTTGCGCt < 1:2126256/73‑1 (MQ=255) cGATTCTTTCCCCTTCCCCCCTGCCCACAGCCCACCCCGGCTTATTCCCCCCCCTGCCATTATTGGCCTTTGCGCt < 1:995347/76‑1 (MQ=255) cGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCt > 2:497683/1‑76 (MQ=255) gATTCGTCCCGCTTCCTCGCTGACGACAGCGCAAACCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTa < 1:6173274/76‑1 (MQ=255) ttCGTTCCGCTTCCCCGCGGACGACACCCAAACCCGGTTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAAt < 2:4358647/76‑1 (MQ=255) cGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGGCATTATTGGCCTTTGCGCTCAtct > 2:5841165/1‑76 (MQ=255) cGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAAtct > 2:3190497/1‑76 (MQ=255) ttccgcttccgcGCTGACGGCAACGCAATCTGGTTTTTTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCa < 2:3673268/76‑1 (MQ=255) tCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCAc > 2:1721005/1‑76 (MQ=255) ccGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCAcc > 1:4442146/1‑76 (MQ=255) ccGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCAcc > 1:117691/1‑76 (MQ=255) cccTTCCTCCCTGACGACCGCCCAAACCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCAcca < 2:4520961/74‑2 (MQ=255) gCTTCCCCGCTGACGACAGCGCAAACCGGCTTATTGACCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCtt < 2:4515393/76‑1 (MQ=255) gCTTCCCCGCGGACGACGGCACAAACCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCtt < 1:5499228/76‑1 (MQ=255) ttACTCGCTGACGACAGCGCAAACCGGCTTATTGACCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTg < 1:4707499/76‑1 (MQ=255) ttACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTg > 2:2416291/1‑76 (MQ=255) ttACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCAACTTTg > 1:1077209/1‑76 (MQ=255) ttACTCGCTGACGACAGCGCAAACCGGCTTATGGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTg < 2:4640332/76‑1 (MQ=255) tACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTgg > 1:4226990/1‑76 (MQ=255) tACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTgg > 1:3048062/1‑76 (MQ=255) tACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTgg > 2:2746094/1‑76 (MQ=255) aCTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGc > 1:4287345/1‑76 (MQ=255) cccGCTGCCGACGGCGCAACCCGTTTTATTCCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCt < 1:1105761/74‑1 (MQ=255) ccgcgGACGACAGCGCAACCCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTg < 2:5295130/75‑1 (MQ=255) ccccTGCCGCCAGCGCAACCCGGCTTATGGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTg < 2:1051824/73‑1 (MQ=255) cTGACGACAGCGCAAACGGTCTTATTGACCACCCCGCCATTATTGGCCTTTGCGCTAATCTCACCTGTGCCTGgcc > 2:2487471/1‑76 (MQ=255) tGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGcccc > 2:5945925/1‑76 (MQ=255) ggacgacAGCACAACCCGGTTTATGGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGcccc < 1:2302195/75‑1 (MQ=255) gacgacCGCGCAAACCGGTTTTTTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCg < 2:2759817/76‑1 (MQ=255) gacgacAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCg > 2:1569277/1‑76 (MQ=255) gacgacAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCg > 2:1465745/1‑76 (MQ=255) gacgacAGCCCAACCCGGTTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCg < 1:3157759/76‑1 (MQ=255) cAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCCGTGgc > 2:2930300/1‑76 (MQ=255) gcacAACCCGTCTTATTCCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGgcgc < 1:369862/73‑1 (MQ=255) cccAACCCGTCTTATTCCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGcgcg < 1:4335782/74‑1 (MQ=255) cAACCCGGTTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGAc < 2:6244592/76‑1 (MQ=255) cAAACCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGAc < 1:6297171/76‑1 (MQ=255) aaaCCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACg > 1:2396634/1‑76 (MQ=255) aaaCCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACg > 1:3986512/1‑76 (MQ=255) aaCCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGt > 2:1089545/1‑76 (MQ=255) aCCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGtt > 1:3199841/1‑76 (MQ=255) aCCGGCTTATCCCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGtt < 1:2743442/76‑1 (MQ=255) ccGGCTTATTCACCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGttt < 2:1849588/76‑1 (MQ=255) gCTTATTCCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTgg < 2:4068573/76‑1 (MQ=255) cTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGt > 1:2663128/1‑76 (MQ=255) cTTATTCCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGt < 2:3222892/76‑1 (MQ=255) ttATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTa > 1:5018021/1‑76 (MQ=255) ttATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTa > 2:361879/1‑76 (MQ=255) tATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTAt > 1:5004958/1‑76 (MQ=255) tATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTAt > 2:1780815/1‑76 (MQ=255) ttGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATgg > 1:5963869/1‑76 (MQ=255) tGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGa > 1:2863199/1‑76 (MQ=255) tGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGa > 1:4445199/1‑76 (MQ=255) tGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGa > 1:4775946/1‑76 (MQ=255) gACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGaa > 2:1450481/1‑76 (MQ=255) gACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGaa < 2:648266/76‑1 (MQ=255) ccaccCTGCCACTATTGGCCATTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGAACg < 2:2682060/76‑1 (MQ=255) | ACCGGCGCAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGAACG > REL606/1852992‑1853140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |