| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,854,401 | 0 | A | C | 45.8% | 76.3 / 47.0 | 83 | K88N (AAA→AAC) | yeaO | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (19/26); new base C (0/38); total (19/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.56e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 102 total aligned reads displayed.
TTTCGCGAGCAATATCTTGCAGAACTGGCGCAACAC‑GAGCAAGAAGGAAAGCGGCTGGCGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTAC > REL606/1854327‑1854474 | tttCGCGAGCAATATCTTGCAGAACTGGTGCAACAC‑GAGCAAGAAGGAAAGCGGCTGGCGGACATCGC‑CAAAAAAc < 1:2080986/76‑1 (MQ=255)tttCGCGAGCAATATCTTGCAGAACTGGCGCAACAC‑GAGCAAGAAGGAAAGCGGCTGGGGGGCAACGC‑Cccaaaaa > 1:521684/1‑75 (MQ=255) ttCGCGAGCAATATCTTGCAGAACTGGCGCAACAC‑GAGCAAGAAGGAAAGCGGCTGGGGGGCAACGCGCaaaaaaa > 2:3578878/1‑75 (MQ=255) ttCGCGAGCAATATCTTGCAGAACTGGCGCAACAC‑GAGCAAGAAGGAAAGCGGCTGGCGGACATCGC‑CAAAAAACa < 1:6194016/76‑1 (MQ=255) ttCGCGAGCAATATCTTGCAGAACTGGCGCAACAC‑GAGCAAGAAGGAAAGCGGCTGGCGGACATCGC‑CAAAAAACa < 2:5502654/76‑1 (MQ=255) ttCGCGAGCAATATCTTGCAGAACTGACGCAACAC‑GAGCAAGAAGGAAAGCGGCTGGCGGACATCGC‑CAAAAAACa < 2:215091/76‑1 (MQ=255) cgcgAGCAATATCTTGCAGAACTGGCGCAACAC‑GAGCAAGAAGGAAAGCGGCTGGCGGACATCGC‑CAAAAAACAGc < 2:3945296/76‑1 (MQ=255) tatCTTGCAGAACTGGCGCAACAC‑GAGCAAGAAGGAAAGCGGCTGGCGGACATAGC‑CAAAAAACAGCCGCTGAccc < 1:600699/76‑1 (MQ=255) atCTTGCAGACCGGGCGCAACAC‑GAGCAAGAAGGAAAGCGTCGGGCGGACACCGC‑CAAAAACCAGCCGCTGACCCt < 2:4723708/76‑1 (MQ=255) atCTTGCAGAACTGGCGCAACAC‑GAGCAAGAAGGAAAGCGGCTGGCGGACATCGC‑CAAAAAACAGCCGCTGACCCt < 1:4451688/76‑1 (MQ=255) atCTTGCAGAACTGGCGCAACAC‑GAGCAAGAAGAAAAGCGGCTGGCGGACATCGC‑CAAAAACCAGCCGCTGACCCt < 2:6139352/76‑1 (MQ=255) accTTGAAGAACGGGCGCACCAG‑GAGCAAAAAGAAAGGCGGCGGGCGGACATCCC‑AAAAAACCAGCCGCTGACCCt < 2:5200859/74‑1 (MQ=255) tCTTGCAGAACTGGCGCAACAC‑GAGCAAGAAGGAAAGCGGCTGGGGGGGATCGC‑CCAAAAAAAACCGCCGAccccc > 1:3569694/1‑74 (MQ=255) tCTGGCAGAACTGGCTCAACAC‑GAGCAAGAAGGAAAGCGGCTGGCGGACATCGC‑CAAAAAACAGCCGCTGACCCTg < 1:1483510/76‑1 (MQ=255) tCTGGCAGAACGGGCGCAACAC‑GAAAAAGAAGAAAAGCGGCGGGGGGACATCGC‑CAAAAACCAGCCGCTGACCCTg < 2:3222307/76‑1 (MQ=255) ttGCAGAACTGGCGCAACAC‑GAGCAAGAAGGAAAGCGGCTGGCGGACATCGC‑CAAAAAACAGCCGCTGACCCTGct < 1:4135917/76‑1 (MQ=255) gCAGAACTGGCGCAACAC‑GAGCAAGAAGGAAAGCGGCTGGCGGACATCGC‑AAAAAACCAGCCGCTGACCCTGctct < 2:3219222/76‑1 (MQ=255) cAGAGCTGCCGCAACAC‑GAGCAAGAAGGAAAGCGGCTGGCGGACATCGC‑CAAAAACCAGCCGCTGACCCTGCTCTa < 1:5343026/76‑1 (MQ=255) cAGAACTGGCGCAACAC‑GAGCAAGAAGGAAAGCGGCTGGCGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTa < 1:5198298/76‑1 (MQ=255) cAGAACGGGCGCAACAC‑GAGCAAGAAGGAAAGCGGCTGGCGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTa < 1:3697506/76‑1 (MQ=255) aagagCTGGCGCAACAC‑GAACAAGAGGGAAGGCGGCGGGCGGACATCGC‑CAAAAACCAGCCGCTGACCCTGCTCTa < 2:2444942/75‑1 (MQ=255) gaaaCTGGCGCAACAC‑GAGCAAGAAGGAAAGCGGCTGGCGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTAc < 1:2804163/74‑1 (MQ=255) aGAACTGG‑GCCACACGGAGAAAGAAGGAAGACGGCTGGCGACCACCCC‑CAAAAAACAGCCGCTGACCCTGCTCTAc < 2:299451/76‑1 (MQ=255) aGAACGGGCGCAACAC‑GAGAAAGAAGGAAAGGGGCTGGCGGACATCGC‑CAAAAACCAGCCGCTGACCCTGCTCTAc < 1:5395268/76‑1 (MQ=255) aGAACGGGCGCAACAC‑GAGAAAGAAGGAAAGCGGCTGGCGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTAc < 1:193250/76‑1 (MQ=255) aGAACGGCCGCAACCC‑AAAAAAGAAGAAAAGCGGCTGGCGGACATCCC‑CAAAAACCAGCCGCTGACCCTGCTCTAc < 2:3229656/76‑1 (MQ=255) gagcTGGAGCACCAC‑GGACAAGAGGAAAGGCGGCTGGCGAACATCCC‑AAAAAACCAGCCGCTGACCCTGCTCTACg < 2:4551263/73‑1 (MQ=255) gAACGGGCGCAACAC‑GAGCAAGAGGGAAAGCGGCTGGCGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACg < 1:5846977/76‑1 (MQ=255) cgggCCCAACAC‑GAGCAAGAAGGAAGGCGGCGGGCGGACATCGC‑CAAAAACCAGCCGCTGACCCTGCTCTACgcag < 1:3643637/74‑1 (MQ=255) cgggCACAACAC‑GAGCAAGAAGGAAAGCGGCTGGCGGACATCGC‑CAAAAACCAGCCGCTGACCCTGCTCTACgcag < 2:2183884/74‑1 (MQ=255) cTGGCCCAACAC‑AAGCAAAAAGGAACGCGGTTGGCGGACATCGC‑CAAAAACCAGCCGCTGACCCTGCTCTACgcag < 1:3579856/76‑1 (MQ=255) ggggcAACAC‑GAGCAAGAAGGAAAGGGGCTGGCGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACgcagca < 1:2303948/73‑1 (MQ=255) ggCGCAACAC‑GAGAAAGAAGGAAAGCGGCGGGCGGACATCGC‑AAAAAACCAGCCGCTGACCCTGCTCTACgcagca < 2:474903/76‑1 (MQ=255) gcgcAACAA‑AAGAAAGAAGGAAGGCGGCGGGCGGCCACCGC‑AAAAAACCAGCCGCTGACCCTGCTCTACGCAGCaa < 2:3119445/76‑1 (MQ=255) cgcAACAC‑GAGCAAGAAGGAAAGCGGCTGGCGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCaaa < 1:4362249/76‑1 (MQ=255) cgcAACAC‑GAGCAAGAAGGAAAGCGGCTGGCGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCaaa < 1:5005916/76‑1 (MQ=255) ccaaaCAC‑GAGAAAGAAGGAAAGCGGCGGGCGGACATCGC‑AAAAAACCAGCCGCTGACCCTGCTCTACGCAGCaaa < 2:6020337/73‑1 (MQ=255) gcAACAC‑GAGAAAGAGGGAAAGCGGCTGGCGGACACCGC‑CAAAAACCAGCCGCTGACCCTGCTCTACGCAGCaaaa < 2:4167500/76‑1 (MQ=255) cAACAC‑GAGCAAGAAGGAAAGCGGCGGGCGGACATCGC‑AAAAAACCAGCCGCTGACCCTGCTCTACGCAGCaaaaa < 2:2459666/76‑1 (MQ=255) aaCAC‑GAGAAAGAAGGAAGGCGGCGGGCGGACACCCC‑AAAAAACCAGCCGCTGACCCTGCTCTACGCAGCaaaaaa < 1:4959839/76‑1 (MQ=255) acac‑GAGAAAAAAGGAAAGCGGCTGGCGGAAATCGC‑CAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAac < 1:3622496/76‑1 (MQ=255) cggagaAAAAAGAAAAGTGGTTGGCGGACACCGC‑CAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAacac < 2:2568468/74‑1 (MQ=255) c‑GAGAAAGAAGGAAAGCGGCGGGCGGACATCGC‑CAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAAcacc < 2:475907/76‑1 (MQ=255) gAGCAAGAAGGAAAGCGGCTGGCGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAAcacca < 1:840988/76‑1 (MQ=255) gCAAAAAGGAAAGCGGCTGGCGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACg < 1:3902718/76‑1 (MQ=255) tctaagGAAAGCGGCTGGCGGCCACCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCa < 1:2683783/73‑1 (MQ=255) aagaagGAAAGCGGCTGGCGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCa < 1:4200987/76‑1 (MQ=255) aagaagGAAAGCGGCTGGCGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCa < 1:643042/76‑1 (MQ=255) aagaagAAAAGCGGCTGGCGGACACCGC‑AAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCa < 2:3011637/76‑1 (MQ=255) agaagGAAGGGGGCGGGCGAACAGCGC‑AAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAg < 2:2667227/76‑1 (MQ=255) agaagGAAAGCGGCTGGCGGACATCGC‑CAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAg < 1:1814641/76‑1 (MQ=255) aagAAAAGGGGCTGCGGAACAACGC‑CAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGaa < 2:1088817/76‑1 (MQ=255) aaagAAAGAGCCTGCGGGACACCGC‑CAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGaa < 2:3559283/73‑1 (MQ=255) agGAAAGCGGCTGGCGGACATCGC‑AAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAAc < 1:270027/76‑1 (MQ=255) ggAAGGCGCCGGGCGGACATCGC‑CAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAAcc < 2:5692298/76‑1 (MQ=255) ggAAAGCGGCTGGCGGACATCGC‑CAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAAcc < 1:5584161/76‑1 (MQ=255) ggAAAGCGGCTGGCGGACATCCC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAAcc < 1:3616715/76‑1 (MQ=255) ggAAAGCGGCTGGCGAACAGCCC‑AAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAAcc < 2:550893/76‑1 (MQ=255) aaggCGGCTGGCGGCCATCGC‑AAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCAt < 2:1652877/73‑1 (MQ=255) aaaGCGGCTGGCGGACATCGC‑CAAAAATCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCAt < 1:1588423/76‑1 (MQ=255) aaaGCGGCTGGCGGACATCGC‑AAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCAt < 1:3386516/76‑1 (MQ=255) aaaGCGCCGGGCGGACACCGC‑AAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCAt < 2:3054216/76‑1 (MQ=255) aggCGGTTGGCGGACATCGC‑CAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATg < 2:856018/74‑1 (MQ=255) aaccGGCTGGCGGACAGCGC‑AAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATg < 1:4983710/73‑1 (MQ=255) aaGCGGCGGGCGGACATCGC‑AAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATg < 1:2765777/76‑1 (MQ=255) gCGGGTGGCGGACACCGC‑AAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATgcg < 2:1097607/76‑1 (MQ=255) gCGGCTGGCGGACACCGC‑CAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATgcg < 1:6169275/76‑1 (MQ=255) gCGGCTGGCGGACACCGC‑AAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATgcg < 1:3994951/76‑1 (MQ=255) ggCTGGCGGGCATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCt > 2:5541224/1‑76 (MQ=255) ggCTGGCGGGCATCGC‑CAAAAAACAGCCGCTGACCCTGCTCCAAGCAACACAAAACACCACGCCGAACCATGAGCt > 2:3187053/1‑76 (MQ=255) gCTGGCGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTg > 2:4075936/1‑76 (MQ=255) gCTGGCGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTg > 1:325001/1‑76 (MQ=255) cTGGCGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTgg > 2:191714/1‑76 (MQ=255) cTGGCGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTgg > 1:2865213/1‑76 (MQ=255) tGGCGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGt > 1:2995371/1‑76 (MQ=255) ggCGGACATCGC‑CAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTg < 2:5468844/76‑1 (MQ=255) ggCGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTg > 2:4415053/1‑76 (MQ=255) gCGGACGCCGC‑CAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACGCCACGCAGAACCATGGGCTGGTGc < 2:5869873/76‑1 (MQ=255) cgaaCATCGC‑AAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCt < 2:1811003/73‑1 (MQ=255) cgaaCATCCC‑CAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCt < 2:2751669/73‑1 (MQ=255) cGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCt > 1:3465319/1‑76 (MQ=255) cGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCt > 2:1537452/1‑76 (MQ=255) cGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCt > 2:2599227/1‑76 (MQ=255) cGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCt > 2:1002589/1‑76 (MQ=255) ggACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTg > 1:4732444/1‑76 (MQ=255) ggACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTg > 2:2923897/1‑76 (MQ=255) ggACATCCC‑AAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTg < 2:638107/76‑1 (MQ=255) gaaCATCGC‑CAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTg < 2:4230404/74‑1 (MQ=255) gACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTgg > 2:2707306/1‑76 (MQ=255) gACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTgg > 2:2556000/1‑76 (MQ=255) cATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGcc > 2:2080260/1‑76 (MQ=255) c‑cAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTg < 1:1767647/76‑1 (MQ=255) caaaaaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTg < 2:6307099/74‑1 (MQ=255) cAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTgg > 1:2118610/1‑76 (MQ=255) aaaaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGc < 1:1797053/76‑1 (MQ=255) aaaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCt < 1:5254120/76‑1 (MQ=255) aaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa < 1:5694155/76‑1 (MQ=255) aaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa < 2:1500713/76‑1 (MQ=255) aaaCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTaa > 1:2256550/1‑75 (MQ=255) aaaCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTAc < 1:2798617/76‑1 (MQ=255) | TTTCGCGAGCAATATCTTGCAGAACTGGCGCAACAC‑GAGCAAGAAGGAAAGCGGCTGGCGGACATCGC‑CAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTAC > REL606/1854327‑1854474 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |