| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,044,357 | 0 | A | C | 36.8% | 286.5 / 90.3 | 163 | V284G (GTG→GGG) | rfbD | dTDP‑4‑dehydrorhamnose reductase subunit, NAD(P)‑binding, of dTDP‑L‑rhamnose synthase |
| Reads supporting (aligned to +/- strand): ref base A (92/11); new base C (0/60); total (92/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.05e-33 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 184 total aligned reads displayed.
CATCATGAGCGAGATGCAAAAATTTGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATGGGGTCGAC > REL606/2044282‑2044432 | catcatGAGCGAGATGCAAAAATTTGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCAcaccca > 2:2680971/1‑76 (MQ=255) atcatGAGCGAGATGCAAAAATTTGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCAcacccac > 1:4409705/1‑76 (MQ=255) atcatGAGCGAGATGCAAAAATTTGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCAcacccac > 2:4124375/1‑76 (MQ=255) catGAGCGAGATGCAAAAATTTGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCt < 2:4946836/76‑1 (MQ=255) catGAGCGAGATGCAAAAATTTGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCt < 1:2198537/76‑1 (MQ=255) atGAGCGAGATGCAAAAATTTGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTg < 2:1662491/76‑1 (MQ=255) atGAGCGAGATGCAAAAATTTGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTg < 2:609998/76‑1 (MQ=255) atGAGCGAGATGCAAAAATTTGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTg > 2:3621682/1‑76 (MQ=255) tGAGCNAGATGCAAAAATTTGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGc > 2:154501/1‑76 (MQ=255) tGAGCGAGATGCAAAAATTTGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGc > 1:1824895/1‑76 (MQ=255) gAGCGAGATGCAAAAATTTGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGcc < 1:3646838/76‑1 (MQ=255) gAGCGAGATGCAAAAATTTGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGcc > 1:6306601/1‑76 (MQ=255) cGAGATGCAAAAATTTGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGt > 2:5221952/1‑76 (MQ=255) cGAGATGCAAAAATTTGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGt > 2:5728553/1‑76 (MQ=255) agaTGCAAAATTTTTTAAAGTGGCGGGAGTCGAAATTAATTCTTTGAGCATTCGTTCCACCCCCCCCTGCCAGTCa < 2:3217547/76‑1 (MQ=255) agaTGCAAAAATTTGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCa > 2:1720122/1‑76 (MQ=255) aTGCAAAAATTTGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAgg > 1:1033523/1‑76 (MQ=255) aTGCAAAAATTTGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAgg > 1:3871118/1‑76 (MQ=255) gaaaaatTTTTTTAAATTGCCGTAGTCGAAAATAATTCGTTGAGCATTCGTTTCCCCCCCCCCTGCCAGTCAGGCa < 2:2730170/74‑1 (MQ=255) gCAAAAATTTGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCa > 2:2530502/1‑76 (MQ=255) aaaatTTTGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACCCCCCCCTGCCAGTCAGGCAAg < 2:425876/76‑1 (MQ=255) aaatTTTGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTCCCCCCCCCCCTGCCAGTCAGGCAAGa < 2:519000/76‑1 (MQ=255) attttGTTAAATTCCCGTATTCATAAATAATTCGTTGAGCATTCGTTTCACCCCCCCCTGCCAGTCAGGCAAGACa < 1:3273443/74‑1 (MQ=255) ttttttAAATTGCCGTAGTCTAAAATATTTCGTGAACCATCCGTTTCCCCCCCACCTGCCAGTCAGGCAAGACAAg < 1:1925191/76‑1 (MQ=255) tGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAgcg > 1:2661084/1‑76 (MQ=255) tGTAAAATGGCGGTAGTCGTAAATAATTCGTGGAGCTTTCTTTCCACCCCCCCCTGCCAGTCAGGCAAGACAAgcg < 1:4535389/76‑1 (MQ=255) gTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAgcgc > 2:4655371/1‑76 (MQ=255) taaaaTTCCCGTGTTCGTAAATAATTCGTTGAGCATTCGTTCCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCa < 1:5515421/74‑1 (MQ=255) tAAATTGCCGTAGTTGTAAATAATTCGTTGAGCATTCGTTTCACCCCCACCTGCCAGTCAGGCAAGACAAGCGCaa < 1:4735471/76‑1 (MQ=255) aaaTTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGTGCaaa > 1:3437944/1‑76 (MQ=255) aaTTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAg > 1:1699347/1‑76 (MQ=255) aaTTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAg > 2:6279797/1‑76 (MQ=255) tGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTc > 2:4798486/1‑76 (MQ=255) tGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTc > 1:731169/1‑76 (MQ=255) gCCGTAGTCGTAAATATTTCGTTGAGCATTCTTTTCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTct < 1:3953503/76‑1 (MQ=255) ccGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTctg > 1:4561841/1‑76 (MQ=255) ccGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTctg > 1:4906705/1‑76 (MQ=255) ccGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTctg > 2:290361/1‑76 (MQ=255) cGTAGTCGTAAATAATTCGTTGCGCAATCGTTTCAAACCCACCTGCTAGTCAGGCAAGACCAGCGCAATGTTctgc > 2:841512/1‑76 (MQ=255) gTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTctgct < 2:4687417/76‑1 (MQ=255) gTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTctgct > 1:2542424/1‑76 (MQ=255) tAGTCTAAAATAATTCGTTGAGCATTCTTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTctgctg < 1:4316255/76‑1 (MQ=255) aGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGa > 2:2280066/1‑76 (MQ=255) tgcGTAAATAATTCGTTGAGCTTTCTTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGaa < 1:4744702/74‑1 (MQ=255) gTCGTAAATAATTCGTTGAGCATTCGTTTCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGaa < 2:4034969/76‑1 (MQ=255) gTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGaa > 2:464795/1‑76 (MQ=255) gTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGaa > 2:1610197/1‑76 (MQ=255) gTCATAAATAATTCTTTGAGCATTCGTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGaa < 2:3517020/76‑1 (MQ=255) tCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGaaa > 2:874472/1‑76 (MQ=255) gTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACt > 2:3999850/1‑76 (MQ=255) gTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACt > 1:1339902/1‑76 (MQ=255) gTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACt > 1:5222668/1‑76 (MQ=255) tAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACtt > 1:4144797/1‑76 (MQ=255) tAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACtt > 1:2455910/1‑76 (MQ=255) aaaaaaaTTCGTTGAGCATTCTTTTCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACtt < 2:1371541/75‑1 (MQ=255) aaaTATTTCGTTGAGCATTCGTTCCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACttt < 2:5000146/76‑1 (MQ=255) aaaTAATTCGTTGAGCATTCGTTTCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACttt < 2:1558728/76‑1 (MQ=255) aataatTCGTGGAGCATTCTTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACtttt < 1:3370856/76‑1 (MQ=255) ataatTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTc > 1:2817235/1‑76 (MQ=255) taatTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCt > 1:954877/1‑76 (MQ=255) tttCGTTGGGCATTCGTTTCACACCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGt < 2:6138070/75‑1 (MQ=255) atTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGt > 1:6037337/1‑76 (MQ=255) atTCGTTGACCTTTCGTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGt < 1:5566552/76‑1 (MQ=255) ttCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTa > 2:5968911/1‑76 (MQ=255) tCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTAt > 1:1950687/1‑76 (MQ=255) cGTTGAGCTTTCGTTCCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTAtt < 2:4479044/76‑1 (MQ=255) cGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTAtt > 1:1730024/1‑76 (MQ=255) cGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTAtt > 1:3992484/1‑76 (MQ=255) cGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTAtt > 1:1262005/1‑76 (MQ=255) cGTTGACCATTCTTTCCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTAtt < 1:390474/76‑1 (MQ=255) cGTGGACCATTCGTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTAtt < 2:2490942/76‑1 (MQ=255) gTTGAGCTTTCGTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTg < 1:1212536/76‑1 (MQ=255) gTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTg > 1:6221214/1‑76 (MQ=255) gTTGACCATTCGTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTg < 1:1547523/76‑1 (MQ=255) ttGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGa > 2:1184038/1‑76 (MQ=255) ttGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGa > 2:113531/1‑76 (MQ=255) tggAGCATTCGTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGa < 1:6154412/74‑1 (MQ=255) tGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAg > 1:3146294/1‑76 (MQ=255) tGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAg > 2:960386/1‑76 (MQ=255) tGACCATTCGTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAg < 1:4516165/76‑1 (MQ=255) gAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAgg > 1:4580444/1‑76 (MQ=255) gctttCGTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCg < 1:5656541/73‑1 (MQ=255) gccttCGTTTCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCg < 1:1902197/73‑1 (MQ=255) gCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCg > 1:590736/1‑76 (MQ=255) ttgcGTTTCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGGAACTTTTCTGTATTGAGGCgag < 1:2853311/73‑1 (MQ=255) aTTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCgag > 2:3256455/1‑76 (MQ=255) aTTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCgag > 2:6159111/1‑76 (MQ=255) tggTTTCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGagag < 2:4409817/74‑1 (MQ=255) tCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGagag > 1:2197279/1‑76 (MQ=255) cGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGt > 1:4691553/1‑76 (MQ=255) cGTTCCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGagaga < 2:5934434/76‑2 (MQ=255) gTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGtt > 1:1030838/1‑76 (MQ=255) gTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGtt > 2:4592825/1‑76 (MQ=255) tttCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTa > 2:1292052/1‑76 (MQ=255) tttCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTa > 1:4928702/1‑76 (MQ=255) ttCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTAt > 2:2902507/1‑76 (MQ=255) ttCACACCCACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTAt > 2:1920042/1‑76 (MQ=255) caccCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATgg < 2:5666124/76‑1 (MQ=255) acccaccTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATGGGGt > 2:2341757/1‑76 (MQ=255) acccaccTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATGGGGt > 1:2990105/1‑76 (MQ=255) | CATCATGAGCGAGATGCAAAAATTTGTTAAATTGCCGTAGTCGTAAATAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATGGGGTCGAC > REL606/2044282‑2044432 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |