| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,774,914 | 0 | C | G | 41.5% | 161.0 / 128.1 | 130 | A20P (GCC→CCC) | yicJ | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base C (0/76); new base G (54/0); total (54/76) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.73e-38 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 164 total aligned reads displayed.
GGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCTGCTTTTCCTTGATG > REL606/3774840‑3774987 | ggCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTggcg < 2:4282974/76‑1 (MQ=255)ggCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTggcg < 2:6191596/76‑1 (MQ=255) cAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTgggggg > 2:4963447/1‑75 (MQ=255) aGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTggggggg > 1:5551022/1‑74 (MQ=255) gAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGtc < 2:4079041/76‑1 (MQ=255) gAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGtc < 2:3811266/76‑1 (MQ=255) aaTGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGtct < 2:1647879/76‑1 (MQ=255) ccAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTcccc > 2:3733113/1‑75 (MQ=255) ccAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCa < 2:2070228/76‑1 (MQ=255) ccAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCa < 1:5996082/76‑1 (MQ=255) ccAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCa < 2:2010933/76‑1 (MQ=255) cAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCAt < 1:3388413/76‑1 (MQ=255) cAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCAt < 2:3701076/76‑1 (MQ=255) aaaaaTATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATg < 1:5410663/76‑1 (MQ=255) aaaaaTATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATg < 1:367633/76‑1 (MQ=255) aaaaaTATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATg < 1:537473/76‑1 (MQ=255) tatCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑a < 1:84515/76‑1 (MQ=255) atCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑aa < 2:4881048/76‑1 (MQ=255) cGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑cc < 1:1269804/76‑1 (MQ=255) ggTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCCGCCAT‑AA‑ACa > 1:3743638/1‑76 (MQ=255) tataAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCGCCCCAGCCCT‑AA‑CCAAt > 2:827440/1‑76 (MQ=255) tataAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCCCCCATGCCCT‑AA‑ACAAt > 1:1535251/1‑76 (MQ=255) ataAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAAtt < 1:2784253/76‑1 (MQ=255) taAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAAttt < 1:3151754/76‑1 (MQ=255) aaaaGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCT‑AA‑CCAAattt > 2:2905867/1‑76 (MQ=255) aaGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCAT‑AACCCAAttttt > 2:3840714/1‑75 (MQ=255) aGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCT‑AA‑ACAAATTtttc > 1:6255929/1‑76 (MQ=255) aGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCCCCCCTGCCCT‑AA‑CCAAttttttt > 1:4074089/1‑73 (MQ=255) aCATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCACGCCCT‑AA‑CCAAATTTCTC‑ttt > 2:2844416/1‑76 (MQ=255) aCATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑ttt < 1:1633109/76‑1 (MQ=255) aCATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑ttt < 1:4697702/76‑1 (MQ=255) aCATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑ttt < 1:3634233/76‑1 (MQ=255) catcatATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCCCCCCTGCCAT‑AA‑CCAATTTTCTC‑tttt > 2:6132407/1‑75 (MQ=255) catcatATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCCCCCATCACCC‑AA‑CCAAttttttt‑tttt > 2:3870472/1‑75 (MQ=255) catcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTa < 1:5256007/76‑1 (MQ=255) catcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTa < 2:1708978/76‑1 (MQ=255) atcatATATAACAGTACGTTATCGAAAATAATGTGGCTGGCGGCGACTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTaa < 1:1360123/76‑1 (MQ=255) tcatATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCCCCCCT‑AA‑ACAACTTTCTC‑ttttaa > 2:3148267/1‑73 (MQ=255) atatatAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCTCAA‑CCAA‑TTTCTC‑TTTTAcag > 2:3806427/1‑74 (MQ=255) atatatAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCT‑AA‑CCACATTTCTC‑TTTTAcag > 2:1948757/1‑74 (MQ=255) tatataACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGGTCCCCCGCCCT‑AA‑CCCAATTTTTC‑TTTTACAGa > 2:591870/1‑76 (MQ=255) tatataACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCT‑AA‑ACAAATTTTTT‑TTTTACGGa > 1:1879996/1‑76 (MQ=255) tatataACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCAGCCAT‑AA‑CCAATTTTTTT‑TTTTACGGa > 1:5736782/1‑76 (MQ=255) atataACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCAT‑AA‑ACAATTTTTTC‑TTTTACgggc > 2:5056333/1‑74 (MQ=255) atataACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCCGCCCT‑AA‑ACAATTTTCTCTTTTAACggc > 2:2782059/1‑75 (MQ=255) tataACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGaca < 1:5259063/76‑1 (MQ=255) tataACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGaca < 1:6231658/76‑1 (MQ=255) tataACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGaca < 1:4460133/76‑1 (MQ=255) taACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCT‑TA‑ACAACTTTTTC‑TTTTACGGacaac > 1:1162843/1‑76 (MQ=255) taACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCT‑AA‑CCAAATTTTTC‑TTTTACCGaccac > 2:1991654/1‑76 (MQ=255) taACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGacaac < 1:3550046/76‑1 (MQ=255) taACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGacaac < 2:2505306/76‑1 (MQ=255) aaCATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCT‑AA‑CCCAATTTTTC‑TTTTAAAGacaaca > 1:6140603/1‑76 (MQ=255) aaCATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCAT‑AA‑CCAAATTTTTC‑TTTTACGGgccaca > 1:1010799/1‑76 (MQ=255) aaCATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGacaaca < 2:5789272/76‑1 (MQ=255) aCATTACGTTATCGAAAATAATGTGGCTGGGGGCGGGCCCCCTCGCAT‑AA‑ACAAATTTCTT‑TTTTAAGGacaaaaa > 1:5508663/1‑73 (MQ=255) cATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCCCGCCCC‑AA‑CCACATTTCTC‑TTTAACTGAAAAAA‑Ct > 1:2977383/1‑76 (MQ=255) aTTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCAT‑AA‑ACAATTTTTTT‑TTTTACTGACAACA‑Ctt > 1:2274585/1‑76 (MQ=255) aTTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑Ctt < 1:603217/76‑1 (MQ=255) aTTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑Ctt < 2:4987642/76‑1 (MQ=255) ttACGTTATCTAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTc < 1:4132545/76‑1 (MQ=255) ttACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCT‑AA‑CCAATTTTCTC‑TTTTAAGGACAACA‑CTTc > 2:1212370/1‑76 (MQ=255) ttACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCC‑AA‑CCCATTTTTTC‑TTTTACCGGCAACA‑ATTc > 1:1994403/1‑76 (MQ=255) ttACGTTATCGAAAATAATGTGGCTGGGGGCGGCTCCCCTGCCCT‑AA‑CCAATTTTTTC‑TTTTACGGACAACA‑CTTc > 1:1659661/1‑76 (MQ=255) ttACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTc < 1:4609449/76‑1 (MQ=255) tACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCT‑AA‑CCAAATTTTTC‑TTTTACGGGCAACA‑CTTCa > 1:5789265/1‑76 (MQ=255) tACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCa < 2:1585712/76‑1 (MQ=255) aCGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCAT‑AA‑CCAATTTTTTC‑TTTTACGGACAACA‑Ctttcc > 1:3244408/1‑73 (MQ=255) aCGTTATCGAAAATAATGTGGCTGGCGGGGGCTCCCATGCCCC‑AA‑ACAATTTTTTC‑TTTTACGGCACACA‑CTTCAc > 1:5859205/1‑76 (MQ=255) ttATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCC‑AA‑CCCCATTTTTC‑TTTAACGGACAAAA‑CCTCActcc > 2:1168386/1‑75 (MQ=255) ttATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCAT‑AA‑CCAATTTTTTC‑TTTTAACGACAACA‑ATTCActct > 1:4275949/1‑76 (MQ=255) tATCGAAAATAATGTGGCTGGGGGGGGGTCCCCCGCCCT‑AA‑CCCAATTTTTC‑TTTTATAGGCAACA‑CCTCACTCtt > 2:5839715/1‑76 (MQ=255) accGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTc < 2:137520/74‑1 (MQ=255) tCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCa < 2:104640/76‑1 (MQ=255) cGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCAt < 2:679829/76‑1 (MQ=255) cGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCAt < 1:3344641/76‑1 (MQ=255) aaaaTAATTTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCt < 2:571726/76‑1 (MQ=255) aaaaTAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCt < 1:3736984/76‑1 (MQ=255) aaaaTAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCt < 2:6130188/76‑1 (MQ=255) aaaTAATGTGGCTGGGGGGGGCTCCCCCGCCAT‑AA‑CCAATTTTTTC‑TTTTACGGACAACA‑CCTCACTCTTCctctc > 2:5847453/1‑75 (MQ=255) aaaTAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCTg < 1:2894065/76‑1 (MQ=255) aaaTAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCTg < 2:4387314/76‑1 (MQ=255) aaaTAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCTg < 2:212047/76‑1 (MQ=255) aataatGTGGCTGGGGGGGGCTCCCCCGCCCT‑AA‑ACAATTTTTTC‑TTTTAACGAAAACACCTTCAC‑CTTCctcttc > 2:839804/1‑74 (MQ=255) ataatGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCTGCt < 2:2367309/76‑1 (MQ=255) taatGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCTGCtt < 1:468223/76‑1 (MQ=255) aatGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCTGCttt < 2:2477584/76‑1 (MQ=255) atGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCTGCtttt < 1:2773066/76‑1 (MQ=255) gtgGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCTGCTTTTcc < 2:3426665/76‑1 (MQ=255) gtgGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCTGCTTTTcc < 1:5583003/76‑1 (MQ=255) tggctggcGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCTGCTTTTCCt > 1:923956/1‑76 (MQ=255) ggctggcGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCTGCTTTTCCtt < 2:2430407/76‑1 (MQ=255) gctggcGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCTGCTTTTCCTTg < 1:374963/76‑1 (MQ=255) gctggcGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCTGCTTTTCCTTg < 1:2524104/76‑1 (MQ=255) ctggcGGCGTCTCCCATGCCAT‑AA‑CCATTTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCTGCTTTTCCTTGa > 2:2873712/1‑76 (MQ=255) ctggcGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCTGCTTTTCCTTGa > 2:2570450/1‑76 (MQ=255) ctggcGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCTGCTTTTCCTTGa > 1:5919255/1‑76 (MQ=255) tggcGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCTGCTTTTCCTTGAt < 2:3713974/76‑1 (MQ=255) tggcGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCTGCTTTTCCTTGAt < 1:5000088/76‑1 (MQ=255) ggcggcGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCTGCTTTTCCTTGATg > 2:2300312/1‑76 (MQ=255) | GGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AA‑CCAATTTTCTC‑TTTAACGGACAACA‑CTTCACTCTTCATCTGCTTTTCCTTGATG > REL606/3774840‑3774987 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |