BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	66,630	A→G	L652P (CTA→CCA)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	66,630	0	A	G	98.9%	349.9 / ‑4.6	88	L652P (CTA→CCA)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (16/71); total (16/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

Only 100 of 101 total aligned reads displayed.

ACCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATAGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGTTTCCAGCCCTTTAAACACCATCC  >  REL606/66557‑66702

ccccGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGc                                                                          <  2:4438687/75‑1 (MQ=255)

  ccGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTc                                                                        <  1:77667/76‑1 (MQ=255)

   cGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCCCTCCGGGAGGTGCGCAGGGATGGCTcc                                                                       >  1:1411361/1‑76 (MQ=255)

    gCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCct                                                                      <  2:4729896/76‑1 (MQ=255)

    gCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCct                                                                      <  2:4346222/76‑1 (MQ=255)

    gCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCct                                                                      <  1:5077903/76‑1 (MQ=255)

     ccATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCctg                                                                     <  1:5542267/76‑1 (MQ=255)

      cATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCctgc                                                                    <  2:4732790/76‑1 (MQ=255)

       aTCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCctgct                                                                   <  1:3793809/76‑1 (MQ=255)

        tCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCctgctg                                                                  <  2:5045179/76‑1 (MQ=255)

        tCAGGTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCctgctg                                                                  <  2:705070/76‑1 (MQ=255)

          aGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGaa                                                                <  1:1704558/76‑1 (MQ=255)

           gTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGaaa                                                               <  2:1601618/76‑1 (MQ=255)

           gTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGaaa                                                               <  1:3079785/76‑1 (MQ=255)

             ttGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAAct                                                             <  1:4671262/76‑1 (MQ=255)

              tGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAActg                                                            <  2:2750596/76‑1 (MQ=255)

               gTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAActgc                                                           <  1:857143/76‑1 (MQ=255)

                   aTGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTggg                                                       <  2:5530141/76‑1 (MQ=255)

                     ggTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGcc                                                     <  1:843870/76‑1 (MQ=255)

                     ggTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGcc                                                     <  2:1412686/76‑1 (MQ=255)

                     ggTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGcc                                                     <  2:4079151/76‑1 (MQ=255)

                      gTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCa                                                    <  2:3533646/76‑1 (MQ=255)

                      gTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCa                                                    <  1:2053337/76‑1 (MQ=255)

                      gTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCa                                                    <  2:5130689/76‑1 (MQ=255)

                       tttCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAg                                                   <  2:4033427/76‑1 (MQ=255)

                       tttCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAg                                                   <  1:613177/76‑1 (MQ=255)

                       tttCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAg                                                   <  1:5167260/76‑1 (MQ=255)

                        ttCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAgc                                                  <  2:1029715/76‑1 (MQ=255)

                        ttCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAgc                                                  <  2:3658353/76‑1 (MQ=255)

                        ttCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAgc                                                  <  2:2232528/76‑1 (MQ=255)

                         tCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAgcg                                                 <  1:3121674/76‑1 (MQ=255)

                         tCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAgcg                                                 <  1:3722133/76‑1 (MQ=255)

                          cgcgTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAgcgg                                                <  2:6196673/76‑1 (MQ=255)

                             gTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGt                                             <  1:4563516/76‑1 (MQ=255)

                              tACATATTCCTGATATGGCTCCCTGCGGGAGATGGGGAGGGATAGATCCCTCCCGAACAGCTGGGGCCgcggccgc                                            >  2:2466588/1‑73 (MQ=255)

                               tcATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTcc                                           <  2:1113896/75‑1 (MQ=255)

                               aCATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTcc                                           <  2:5220056/76‑1 (MQ=255)

                               aCATATTCCTGATATGGCTCGTGGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACGGCTGGGCCAGCGGCGTcc                                           <  2:4300658/76‑1 (MQ=255)

                               aCATATTCCTGATATGGCTCCCTGCGGGAGATGGGCAGGGATGGGTCCCCCCGCAACGGCTGGGGCGGCGGCGGcc                                           >  1:3512636/1‑76 (MQ=255)

                                cATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCa                                          <  2:6316731/76‑1 (MQ=255)

                                 atatTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAg                                         <  1:3861935/76‑1 (MQ=255)

                                 atatTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAg                                         <  2:6082808/76‑1 (MQ=255)

                                 atatTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAg                                         <  1:6317300/76‑1 (MQ=255)

                                  tatTCCTGATATGGCTCGTGGCGGAAGATGCTCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGt                                        <  1:1253217/76‑1 (MQ=255)

                                   atTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTc                                       <  1:5669052/76‑1 (MQ=255)

                                   atTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTc                                       <  1:5560355/76‑1 (MQ=255)

                                    ttCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATAGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCg                                      <  1:2795540/76‑1 (MQ=255)

                                     tCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCgg                                     <  1:5176947/76‑1 (MQ=255)

                                      ccTGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGt                                    <  2:28622/76‑1 (MQ=255)

                                        tGATATGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTgc                                  <  2:2958521/76‑1 (MQ=255)

                                          atgtGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTgcgc                                <  2:4929595/73‑1 (MQ=255)

                                           tatGGCTCGTTGGGGAGTATGCGCTGGTAGCGCTCCTACTGAAACGGCTGGCCCAGCGGCGTCCAGTCGGTGCGCa                               <  2:4321410/76‑1 (MQ=255)

                                           tatGGCTCGTGGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCa                               <  1:5238580/76‑1 (MQ=255)

                                            atGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑c                             <  1:4326323/76‑1 (MQ=255)

                                             tGGCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑g                           <  1:3642131/76‑1 (MQ=255)

                                              ggCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑gg                          <  1:3765540/76‑1 (MQ=255)

                                              ggCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑gg                          <  1:2421557/76‑1 (MQ=255)

                                               gCTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGt                         <  2:514060/76‑1 (MQ=255)

                                                ctctTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGtt                        <  1:299348/76‑1 (MQ=255)

                                                ctctTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGtt                        <  1:2585175/76‑1 (MQ=255)

                                                cTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGGCAGCGGGGGCCAGTCGGCGCGCG‑C‑gggt                        >  1:1070425/1‑74 (MQ=255)

                                                cTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGtt                        <  2:5237909/76‑1 (MQ=255)

                                                cTCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGGCGGTGCGCA‑C‑gggt                        >  1:1040724/1‑74 (MQ=255)

                                                 tCGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGttt                       <  1:4925744/76‑1 (MQ=255)

                                                 tCGGTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAACCTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGttt                       <  2:5471523/76‑1 (MQ=255)

                                                  cGTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGTTTc                      <  1:3546739/76‑1 (MQ=255)

                                                  cGTTGCGGAAGATGCGCAGGTATGGCTCCCGCTGAAAATGCTGGGCCAGCGGCGGCCCGTCCGGGCGCG‑C‑GGGTTc                      >  2:4503144/1‑76 (MQ=255)

                                                   gTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGGCAGCGGCGTCCCGTCGGTGCGCC‑C‑GGGTTcc                     >  1:4416009/1‑76 (MQ=255)

                                                   gTTGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGTTTcc                     <  1:3583807/76‑1 (MQ=255)

                                                   gTTGCGGAAGATGCGCAGGTATGGCTCCCCCCGAAACTGCTGGGGCCGCGGCGGCCAGTGCGTGCGCC‑C‑GGgtttc                     >  2:3286944/1‑74 (MQ=255)

                                                    ttGTGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGTTTCCa                    <  1:752928/76‑1 (MQ=255)

                                                    ttGCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGTTTCCa                    <  1:3323400/76‑1 (MQ=255)

                                                     tGCGGAAGATGCGCAGGTATGGCTCCTGCTTTAACTGCTGGGCGCGCGGGGGCCAGGCGGGGCGGACC‑GGTTTctg                    >  1:2038686/1‑74 (MQ=255)

                                                      gCGGAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGTTTCCAGc                  <  1:5917208/76‑1 (MQ=255)

                                                      gCGGAAGATGCGCAGGTATGGCTCCTGCGGAAACTTCTGGGCCGGCGGCGTCCAGTCGGTGCGCA‑C‑GGTTTCCAGc                  <  2:2040469/76‑1 (MQ=255)

                                                      gCGGAAGATGCGCAGGTATGGCTCCCGCCGAAAACGCCGGGCCCGCGGCGCCCAGCCGGGGGGCC‑CGGGTTTCCac                   >  2:1651040/1‑75 (MQ=255)

                                                        ggAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCCGTCGGTGGGCA‑C‑GGGTTCCCGccc                >  1:724750/1‑76 (MQ=255)

                                                        ggAAGATGCGCAGGTATGGCTCCCGCTGAAACTGCTGGGCCAGCGGCGTCCCGTCGGTGCGCC‑C‑GGGTTTCAGccc                >  2:3613718/1‑76 (MQ=255)

                                                         gAAGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGGTTCCAGCCCt               >  1:1335608/1‑76 (MQ=255)

                                                          aaGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGTTTCCAGCCCtt              >  1:6246227/1‑76 (MQ=255)

                                                          aaGATGCGCAGGTATGGCTCCCGCTGAAACTGCTGGGGCCGCGGCGTCCAGGCGGTGGGCG‑C‑GGTTTCCAGCCCtt              >  2:246842/1‑76 (MQ=255)

                                                           aGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGTTTCCAGCCCttt             <  2:3343329/76‑1 (MQ=255)

                                                           aGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGTTTCCAGCCCttt             <  2:342761/76‑1 (MQ=255)

                                                           aGATGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGTTTCCAGCCCttt             <  1:3706997/76‑1 (MQ=255)

                                                             aTGCGCAGTTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGTTTCCAGCCCTTTaa           <  1:4506723/76‑1 (MQ=255)

                                                             aTGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGGTTTCAGCCCcttta           >  1:1063666/1‑74 (MQ=255)

                                                              tGCGCAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGTTTCCAGCCCTTTaaa          <  2:1222365/76‑1 (MQ=255)

                                                              tGCGCAGGTATGGCTCCCGCTGAAACTGCTGGGCCAGCGGCGGCCCAGCGGTGCTCA‑C‑GGTTTCCACGCCTTTaaa          >  2:6156436/1‑76 (MQ=255)

                                                              tGCGCAGGTATGCCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGTTTCCAGCCCTTTaaa          <  1:2213736/76‑1 (MQ=255)

                                                                cgcAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGTTTCCAGCCCTTTAAaca        <  1:2394759/76‑1 (MQ=255)

                                                                cgcAGGTATGGCTCCCGCTGAAACTGCTGGGCCCGCGGCGTCCCGTCGGGGGGCC‑C‑GGGTTTCAGCCCCTTTAaca        >  2:4534182/1‑76 (MQ=255)

                                                                 gcAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGTTTCCAGCCCTTTAAacac       <  1:4089982/76‑1 (MQ=255)

                                                                 gcAGGTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGTTTCCAGCCCTTTAAacac       <  2:1040724/76‑1 (MQ=255)

                                                                   gggTATGGCTCTTGCGGAAGCTGATGGGCCTGTTGCGTCCAGTCGGTGCGCA‑C‑GGTTTCCAGCCCTTTAAACACCa     <  2:3173122/75‑1 (MQ=255)

                                                                   aGGTATAGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGTTTCCAGCCCTTTAAACACCa     <  1:841101/76‑1 (MQ=255)

                                                                   aGGTAGGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGTTTCCAGCCCTTTAAACACCa     <  1:1458514/76‑1 (MQ=255)

                                                                    ggTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGTTTCCAGCCCTTTAAACACCAt    <  1:3902466/76‑1 (MQ=255)

                                                                    ggTATGGCTCCCGCTGAAACTGCTGGGGCAGCGGCGTCCCGTCGGTGCGCA‑C‑GGGTTCCCGCCCTTTTAACACCAt    >  2:4047592/1‑76 (MQ=255)

                                                                     gTATGGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGTTTCCAGCCCTTTAAACACCATc   <  1:3152394/76‑1 (MQ=255)

                                                                      tatgGCTCCCGCTGAAAATGCTGGGCCAGCGGCGTCCCGTCGGTGGGGA‑C‑GGGTTCCAGCCCCTTAAACACCAccc  >  2:6121938/1‑76 (MQ=255)

ACCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATAGCTCCTGCTGAAACTGCTGGGCCAGCGGCGTCCAGTCGGTGCGCA‑C‑GGTTTCCAGCCCTTTAAACACCATCC  >  REL606/66557‑66702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`