| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 649,391 | T→G | I471L (ATC→CTC) | mrdA ← | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 649,391 | 0 | T | G | 98.8% | 680.5 / ‑2.8 | 165 | I471L (ATC→CTC) | mrdA | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
| Reads supporting (aligned to +/- strand): ref base T (2/0); new base G (89/74); total (91/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
Only 100 of 179 total aligned reads displayed.
GCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGT > REL606/649319‑649462 | gCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGagcat < 1:679626/76‑1 (MQ=255)gCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGagcat < 1:1908704/76‑1 (MQ=255)gCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGagcat < 1:1826439/76‑1 (MQ=255) cACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGagcatc < 1:3228338/76‑1 (MQ=255) aCCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAgcatca < 2:800871/76‑1 (MQ=255) aCCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAgcatca < 2:4294055/76‑1 (MQ=255) ccTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAg < 2:4110522/76‑1 (MQ=255) ccTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAg > 1:2220690/1‑76 (MQ=255) cTGTTTGCCGTCTTCGGCGGTGCTCATCAGCACATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGt > 2:490457/1‑76 (MQ=255) cTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGt > 2:5895936/1‑76 (MQ=255) tGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTg < 1:882783/76‑1 (MQ=255) gTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGc > 1:38961/1‑76 (MQ=255) gTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGc > 1:422138/1‑76 (MQ=255) tttGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGcc > 2:3096382/1‑76 (MQ=255) tttGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGcc < 1:2860634/76‑1 (MQ=255) ttGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCt > 2:1493179/1‑76 (MQ=255) ttGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCt < 1:62726/76‑1 (MQ=255) tGCCGTCTTCGGCGGTGCTCGTCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCtt < 2:88842/76‑1 (MQ=255) tGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGGACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCtt > 2:2845586/1‑76 (MQ=255) tGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCtt > 1:2891153/1‑76 (MQ=255) tGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCtt > 1:2785685/1‑76 (MQ=255) tGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCtt > 2:5440246/1‑76 (MQ=255) tGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCtt > 1:3642494/1‑76 (MQ=255) tGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCtt < 2:4655024/76‑1 (MQ=255) gCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTa > 2:260277/1‑76 (MQ=255) gCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTa < 2:1973419/76‑1 (MQ=255) gCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTa > 2:2428245/1‑76 (MQ=255) gCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTa > 2:4730466/1‑76 (MQ=255) ccGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTAc > 2:4333971/1‑76 (MQ=255) ccGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTAc > 2:6055832/1‑76 (MQ=255) gTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTc > 2:4542987/1‑76 (MQ=255) tCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCa < 1:965833/76‑1 (MQ=255) cTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCAt < 2:4344805/76‑1 (MQ=255) cTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCAt > 1:6154128/1‑76 (MQ=255) cTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCAt > 1:132128/1‑76 (MQ=255) cTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCAt < 2:3970852/76‑1 (MQ=255) tCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCt < 1:2651859/76‑1 (MQ=255) tCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCt > 2:2547573/1‑76 (MQ=255) ggcggTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTgg < 1:2519991/76‑1 (MQ=255) ggcggTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTgg < 1:5635507/76‑1 (MQ=255) gcggTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGa < 1:3935861/76‑1 (MQ=255) gcggTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGa < 2:236309/76‑1 (MQ=255) ggTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGAtt < 2:6300084/76‑1 (MQ=255) ggTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGAtt < 2:2740982/76‑1 (MQ=255) gTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTg < 2:1839341/76‑1 (MQ=255) gCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTggg > 1:367159/1‑76 (MQ=255) tcatcaGCAAATGCGGAACCTTCACGATACCTTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGt < 2:2843205/76‑1 (MQ=255) tcatcaGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGt > 2:1752046/1‑76 (MQ=255) catcaGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTc < 2:4993864/76‑1 (MQ=255) atcaGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCg > 1:4636451/1‑76 (MQ=255) atcaGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCg < 1:1990982/76‑1 (MQ=255) tcaGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGc > 2:3008896/1‑76 (MQ=255) aGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTg > 2:4732716/1‑76 (MQ=255) aGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTg > 1:5284151/1‑76 (MQ=255) cAAATGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTc > 1:5173101/1‑76 (MQ=255) aaaTGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTcc < 2:4040124/76‑1 (MQ=255) aaaTGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTcc > 1:4687899/1‑76 (MQ=255) aaTGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCa > 2:3473928/1‑76 (MQ=255) aaTGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCa > 1:3818019/1‑76 (MQ=255) tGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGt < 1:104025/76‑1 (MQ=255) tGCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGt > 1:3973100/1‑76 (MQ=255) gCGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTa > 1:5927281/1‑76 (MQ=255) gCGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGGCGCTGTCCAGTa < 2:6227083/76‑1 (MQ=255) cGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTaa < 1:351688/76‑1 (MQ=255) cGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTaa < 1:4522045/76‑1 (MQ=255) cGGAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTaa < 1:5235957/76‑1 (MQ=255) ggAGCCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTacc < 2:812321/76‑3 (MQ=255) ggAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAAc > 2:1291376/1‑76 (MQ=255) ggAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAAc > 2:3190215/1‑76 (MQ=255) gAACCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAAcc > 2:5568975/1‑76 (MQ=255) aaCCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAAccc > 1:1010791/1‑76 (MQ=255) aCCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCt < 1:490457/76‑1 (MQ=255) aCCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCt < 1:4847194/76‑1 (MQ=255) aCCTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCt < 1:1421557/76‑1 (MQ=255) ccTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTg < 2:5875238/76‑1 (MQ=255) cTTCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGa < 2:6007341/76‑1 (MQ=255) ttCACGTTACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGAc < 2:235778/76‑1 (MQ=255) ttCACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGAc > 1:429164/1‑76 (MQ=255) cACGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCg < 1:5531349/76‑1 (MQ=255) cGATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGAt < 2:4875658/76‑1 (MQ=255) gATACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATa < 1:5899678/76‑1 (MQ=255) aTACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATAc < 2:6191829/76‑1 (MQ=255) aTACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATAc > 1:2730286/1‑76 (MQ=255) aTACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATAc < 2:1051778/76‑1 (MQ=255) tACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATAcc > 1:5068276/1‑76 (MQ=255) tACCGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATAcc < 2:1324493/76‑1 (MQ=255) cGTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAAc < 2:5350609/76‑1 (MQ=255) gTCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAAcc > 1:2782629/1‑76 (MQ=255) tCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCg > 2:5199770/1‑76 (MQ=255) tCATTAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCg > 1:1337009/1‑76 (MQ=255) ttAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGaa > 1:3307613/1‑76 (MQ=255) ttAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGaa < 2:1623076/76‑1 (MQ=255) tAATCAGGAGCATCAGTGCCTTCCTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAAt < 1:1735739/76‑1 (MQ=255) tAATCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAAt > 1:1221265/1‑76 (MQ=255) aaTCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATg > 2:2497944/1‑76 (MQ=255) aTCAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATgg > 2:3738122/1‑76 (MQ=255) aTCAGGAGCATCAGTCCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATgg < 1:4320416/76‑1 (MQ=255) cAGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGgtg > 1:5935834/1‑76 (MQ=255) aGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGgtgt < 2:5243327/76‑1 (MQ=255) aGGAGCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGgtgt > 2:3780172/1‑76 (MQ=255) | GCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGCGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGT > REL606/649319‑649462 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |