| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 670,789 | A→G | A452A (GCA→GCG) | ECB_00624 → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 670,789 | 0 | A | G | 98.3% | 706.9 / ‑1.4 | 177 | A452A (GCA→GCG) | ECB_00624 | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base G (48/125); total (50/127) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 179 total aligned reads displayed.
ATGTGGCGGATATCCCGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCACTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGACATTGTACGCATCAATACCATC > REL606/670716‑670861 | aTGTGGCGGATATCCCGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCt < 1:5482913/76‑1 (MQ=255)aTGTGGCGGATATCCCGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCt < 1:4014906/76‑1 (MQ=255)aTGTGGCGGATATCCCGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCt < 2:5127481/76‑1 (MQ=255)aTGTGGCGGATATCCCGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCt < 1:4207677/76‑1 (MQ=255)aTGTGGCGGATATCCCGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCt < 1:3051016/76‑1 (MQ=255)aTGTGGCGGATATCCCGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCt < 1:2588079/76‑1 (MQ=255) tgtgGCGGATATCCCGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCtt < 2:3471004/76‑1 (MQ=255) tgtgGCGGATATCCCGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCtt < 2:2884358/76‑1 (MQ=255) gtgGCGGATATCCCGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCttt < 1:1470981/76‑1 (MQ=255) ggCGGATATCCCGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTcc > 1:3358545/1‑76 (MQ=255) ggCGGATATCCCGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTcc < 2:3862692/76‑1 (MQ=255) ggCGGATATCCCGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTcc < 1:3153536/76‑1 (MQ=255) gCGGATATCCCGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTccc < 1:4235684/76‑1 (MQ=255) ggATATCCCGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCg < 1:3096396/76‑1 (MQ=255) gATATCCCGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCgg < 2:4296564/76‑1 (MQ=255) tatCCCGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGAt < 2:605589/76‑1 (MQ=255) atCCCGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATg < 2:1215639/76‑1 (MQ=255) atCCCGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATg < 2:4162412/76‑1 (MQ=255) atCCCGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATg < 1:4966279/76‑1 (MQ=255) atCCCGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATg < 1:5522557/76‑1 (MQ=255) cccGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATAGCGCTTTCCCCGGATGaa > 1:3323441/1‑76 (MQ=255) gCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGaaaaa > 1:2754116/1‑76 (MQ=255) gCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGaaaaa < 2:6236398/76‑1 (MQ=255) cTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGaaaaaa < 1:5771462/76‑1 (MQ=255) cTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGaaaaaa < 2:5387487/76‑1 (MQ=255) cTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGaaaaaa < 2:631583/76‑1 (MQ=255) cTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGaaaaaa > 2:5086843/1‑76 (MQ=255) tGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAat > 2:2234344/1‑76 (MQ=255) tGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAat < 1:5754877/76‑1 (MQ=255) tGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAat < 1:3664450/76‑1 (MQ=255) tGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAat < 2:2536811/76‑1 (MQ=255) tGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAat < 2:2668826/76‑1 (MQ=255) gagaAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAatat > 1:2279809/1‑76 (MQ=255) agaAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATAtt > 1:3627602/1‑76 (MQ=255) aaaTACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATAttgt < 1:3221140/76‑1 (MQ=255) aaaTACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATAttgt < 1:2002494/76‑1 (MQ=255) aaaTACGACGGGACGGGGACGAGAAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGCTGAAAAAATAttgt > 1:2586277/1‑76 (MQ=255) aTACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATAttgttg < 1:1766599/76‑1 (MQ=255) tACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGa > 2:4711591/1‑76 (MQ=255) tACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGa < 1:5366746/76‑1 (MQ=255) tACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGa < 2:4472090/76‑1 (MQ=255) acgacgGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGAt < 1:544392/76‑1 (MQ=255) gacgggacggGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGTTTTCCCCGGATGGAAAAATATTGTTGATaa > 1:1145799/1‑75 (MQ=255) gacgggacggGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATAc > 1:4851380/1‑76 (MQ=255) acgggacgggGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATAcc < 1:3899494/76‑1 (MQ=255) acgggacgggGACGAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATAcc < 1:103541/76‑1 (MQ=255) gggacggggacgAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCg > 1:2310657/1‑76 (MQ=255) ggacggggacgAGTAATAAACCTGGTGCCTGGGTTATGGCACTTTCCCCGGATGAAAAAATATTGTTGATACCCgg > 1:6165397/1‑76 (MQ=255) acggggacgAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTa > 2:2450393/1‑76 (MQ=255) acggggacgAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTa < 2:6101953/76‑1 (MQ=255) cggggacgAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAGTATTGTTGATACCCGGTAt < 2:4899789/76‑1 (MQ=255) cggggacgAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTAt < 2:2741452/76‑1 (MQ=255) cggggacgAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTAt < 1:6247029/76‑1 (MQ=255) cggggacgAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTAt < 1:2070978/76‑1 (MQ=255) ggggacgAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATg < 1:111279/76‑1 (MQ=255) ggggacgAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATg < 2:1640164/76‑1 (MQ=255) ggggacgAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATg < 2:5505273/76‑1 (MQ=255) ggggacgAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATg < 1:5107530/76‑1 (MQ=255) ggggacgAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAACATTGTTGATACCCGGTATg > 2:2754659/1‑76 (MQ=255) ggacgAGTAATCAACCTGCTGCCTGGGTTATGGCGCGGCCCCCGCATGAAAAAATATTGGTGATACCCGGTATGGt < 2:2894495/76‑1 (MQ=255) ggacgAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGt > 2:3645280/1‑76 (MQ=255) gacgAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTc < 1:1678976/76‑1 (MQ=255) acgAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCa < 1:5761865/76‑1 (MQ=255) acgAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCa > 1:1609842/1‑76 (MQ=255) acgAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCa < 1:3810527/76‑1 (MQ=255) acgAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCa < 1:2673006/76‑1 (MQ=255) cgAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCag < 2:1401050/76‑1 (MQ=255) gAGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCaga < 2:1782909/76‑1 (MQ=255) aGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCagag > 2:2189521/1‑76 (MQ=255) aGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCagag < 2:4265117/76‑1 (MQ=255) aGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCagag > 1:4411557/1‑76 (MQ=255) aGTAATAAACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCagag > 1:1969817/1‑76 (MQ=255) aataaACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTg < 2:4097606/76‑1 (MQ=255) aataaACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTg > 2:5307775/1‑76 (MQ=255) aataaACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTg < 1:1744637/76‑1 (MQ=255) aataaACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTg > 2:1988292/1‑76 (MQ=255) ataaACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGa < 2:5178193/76‑1 (MQ=255) ataaACCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGa < 1:2220986/76‑1 (MQ=255) aaaCCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGACa < 1:5090646/76‑1 (MQ=255) aCCTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGACAtt < 2:1949805/76‑1 (MQ=255) ccTGGTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGACATTg > 1:4878433/1‑76 (MQ=255) ggTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGACATTGTAc < 1:2636216/76‑1 (MQ=255) ggTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGACATTGTAc < 1:5081452/76‑1 (MQ=255) gTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGACATTGTACg < 1:3727982/76‑1 (MQ=255) gTGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGACATTGTACg > 2:5261198/1‑76 (MQ=255) tGCCTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGACATTGTACGc < 1:3455262/76‑1 (MQ=255) ccTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGACATTGTACGCAt < 2:881736/76‑1 (MQ=255) ccTGGGTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGACATTGTACGCAt < 2:4026675/76‑1 (MQ=255) gggTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATGCCCGGTATGGTCAGAGGTGACATTGTACGCATCaa < 2:3119570/76‑1 (MQ=255) gggTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGACATTGTACGCATCaa < 2:4746491/76‑1 (MQ=255) ggTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGACATTGTACGCATCAAt > 2:3321012/1‑76 (MQ=255) ggTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGACATTGTACGCATCAAt < 2:1812298/76‑1 (MQ=255) ggTTATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGACATTGTACGCATCAAt < 1:3768245/76‑1 (MQ=255) tATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGACATTGTACGCATCAATAcc < 1:5160376/76‑1 (MQ=255) tATGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGACATTGTACGCATCAATAcc < 2:3694970/76‑1 (MQ=255) aTGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGACATTGTACGCATCAATACCa > 2:2847848/1‑76 (MQ=255) tGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGACATTGTACGCATCAATACCAt < 1:6072124/76‑1 (MQ=255) tGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGACATTGTACGCATCAATACCAt < 1:4709314/76‑1 (MQ=255) tGGCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATCGTCAGAGGTGACATTGTACGCATCAATACCAt < 2:1583683/76‑1 (MQ=255) ggCGCTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGACATTGTACGCATCAATACCATc < 1:5030261/76‑1 (MQ=255) | ATGTGGCGGATATCCCGCTGGAGAAATACGACGGGACGGGGACGAGTAATAAACCTGGTGCCTGGGTTATGGCACTTTCCCCGGATGAAAAAATATTGTTGATACCCGGTATGGTCAGAGGTGACATTGTACGCATCAATACCATC > REL606/670716‑670861 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |