BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	782,538	A→G	K8R (AAG→AGG)	ybhI →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	782,538	0	A	G	98.1%	870.4 / ‑1.8	209	K8R (AAG→AGG)	ybhI	predicted transporter
Reads supporting (aligned to +/- strand): ref base A (2/2); new base G (104/101); total (106/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

CAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGC  >  REL606/782465‑782610

caatcaatAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGc                                                                        >  2:2167729/1‑76 (MQ=255)

 aatcaatAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCt                                                                       >  2:4362079/1‑76 (MQ=255)

 aatcaatAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCt                                                                       >  2:487573/1‑76 (MQ=255)

 aatcaatAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCt                                                                       <  1:3927246/76‑1 (MQ=255)

 aatcaatAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCt                                                                       >  2:2330235/1‑76 (MQ=255)

  atcaatAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTa                                                                      <  1:3558220/76‑1 (MQ=255)

  atcaatAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTa                                                                      <  1:5638722/76‑1 (MQ=255)

  atcaatAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTa                                                                      >  1:5215397/1‑76 (MQ=255)

  atcaatAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTa                                                                      <  2:3355766/76‑1 (MQ=255)

    caatAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAAt                                                                    >  1:2134524/1‑76 (MQ=255)

     aatAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAAtt                                                                   >  2:2198896/1‑76 (MQ=255)

      atAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTc                                                                  >  1:2068982/1‑76 (MQ=255)

       tAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCt                                                                 <  2:2593127/76‑1 (MQ=255)

       tAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCt                                                                 >  2:597614/1‑76 (MQ=255)

        aaaGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTg                                                                <  2:5865175/76‑1 (MQ=255)

        aaaGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTg                                                                >  1:2965846/1‑76 (MQ=255)

         aaGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGa                                                               <  1:1327663/76‑1 (MQ=255)

          aGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGat                                                              <  1:5273255/76‑1 (MQ=255)

           ggACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGata                                                             >  2:3084916/1‑76 (MQ=255)

              cTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTa                                                          <  1:4360038/76‑1 (MQ=255)

               ttCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAg                                                         <  2:5291649/76‑1 (MQ=255)

               ttCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAg                                                         >  2:5083787/1‑76 (MQ=255)

               ttCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAg                                                         <  2:2029804/76‑1 (MQ=255)

                tCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGc                                                        <  2:444017/76‑1 (MQ=255)

                 cTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCg                                                       <  1:111508/76‑1 (MQ=255)

                 cTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCg                                                       <  1:4064897/76‑1 (MQ=255)

                 cTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCg                                                       >  1:2333535/1‑76 (MQ=255)

                  tGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGa                                                      >  2:4534517/1‑76 (MQ=255)

                  tGTATGAGTCAAACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGa                                                      <  2:1935209/76‑1 (MQ=255)

                   gTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGAt                                                     >  1:3459625/1‑76 (MQ=255)

                   gTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGAt                                                     >  2:1879106/1‑76 (MQ=255)

                   gTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGAt                                                     >  2:5303461/1‑76 (MQ=255)

                    tATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATc                                                    <  1:6189970/76‑1 (MQ=255)

                    tATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATc                                                    >  1:1977677/1‑76 (MQ=255)

                     aTGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATcc                                                   >  2:607799/1‑76 (MQ=255)

                      tGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATccc                                                  <  2:6298380/76‑1 (MQ=255)

                      tGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATccc                                                  >  2:6149609/1‑76 (MQ=255)

                       gAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCa                                                 <  2:3969789/76‑1 (MQ=255)

                       gAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCa                                                 <  1:148904/76‑1 (MQ=255)

                        aGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCAt                                                <  1:4654894/76‑1 (MQ=255)

                        aGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCAt                                                >  1:3531047/1‑76 (MQ=255)

                         gTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATg                                               >  1:3217695/1‑76 (MQ=255)

                         gTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATg                                               <  1:4832173/76‑1 (MQ=255)

                          tCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGt                                              <  1:1584452/76‑1 (MQ=255)

                           cATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGta                                             <  2:3207342/76‑1 (MQ=255)

                            aTACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGtat                                            >  1:1263955/1‑76 (MQ=255)

                             tACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGtatt                                           >  1:5408720/1‑76 (MQ=255)

                               cAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGtattat                                         <  1:2394766/76‑1 (MQ=255)

                                agaaagaaCAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTattatt                                        <  1:1826169/76‑1 (MQ=255)

                                agaaagaaCAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTattatt                                        <  2:816155/76‑1 (MQ=255)

                                agaaagaaCAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTattatt                                        >  1:3685427/1‑76 (MQ=255)

                                 gaaagaaCAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGATCCCATGTATTATTg                                       >  1:4178268/1‑76 (MQ=255)

                                  aaagaaCAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTattatt                                        >  2:897093/1‑74 (MQ=255)

                                  aaagaaCAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTattatt                                        <  1:897093/74‑1 (MQ=255)

                                  aaagaaCAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTgg                                      <  2:140762/76‑1 (MQ=255)

                                  aaagaaCAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTgg                                      >  1:5775395/1‑76 (MQ=255)

                                   aagaaCAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGt                                     >  1:3368497/1‑76 (MQ=255)

                                    agaaCAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGtt                                    >  1:6275075/1‑76 (MQ=255)

                                      aaCAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGtttt                                  >  1:1388005/1‑76 (MQ=255)

                                       aCAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTa                                 <  1:493459/76‑1 (MQ=255)

                                        cAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTAt                                <  2:3459625/76‑1 (MQ=255)

                                        cAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTAt                                >  2:2517766/1‑76 (MQ=255)

                                        cAGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTAt                                <  1:5365241/76‑1 (MQ=255)

                                        cAGGATATTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTAt                                >  1:460452/1‑76 (MQ=255)

                                         aGGATTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATg                               <  2:3322700/76‑1 (MQ=255)

                                         aGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATg                               <  1:4547482/76‑1 (MQ=255)

                                            aTTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCa                            >  1:268901/1‑76 (MQ=255)

                                            aTTTTAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCa                            <  1:250242/76‑1 (MQ=255)

                                             ttttAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAg                           >  1:2781355/1‑76 (MQ=255)

                                             ttttAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAg                           <  1:3875605/76‑1 (MQ=255)

                                              tttAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGc                          >  1:2283947/1‑76 (MQ=255)

                                              tttAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGc                          >  1:703988/1‑76 (MQ=255)

                                               ttAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCt                         >  2:1778071/1‑76 (MQ=255)

                                               ttAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCt                         >  2:2976962/1‑76 (MQ=255)

                                                tAAATGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTc                        >  1:1881659/1‑76 (MQ=255)

                                                 aaaTGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTcc                       >  2:4700077/1‑76 (MQ=255)

                                                 aaaTGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTcc                       <  2:1122150/76‑1 (MQ=255)

                                                   aTGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCgg                     <  1:3636051/76‑1 (MQ=255)

                                                    tGAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGc                    >  2:5674698/1‑76 (MQ=255)

                                                     gAATAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCa                   >  1:5914954/1‑76 (MQ=255)

                                                        tAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGa                <  2:5740632/76‑1 (MQ=255)

                                                        tAAGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGa                <  2:4716159/76‑1 (MQ=255)

                                                         aaGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGAt               >  2:16397/1‑76 (MQ=255)

                                                         aaGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGAt               >  1:5503669/1‑76 (MQ=255)

                                                          aGAAATCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGAtt              >  2:2682904/1‑76 (MQ=255)

                                                            aaaTCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTaa            <  1:5083787/76‑1 (MQ=255)

                                                            aaaTCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTaa            >  1:468494/1‑76 (MQ=255)

                                                             aaTCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAg           <  1:4987349/76‑1 (MQ=255)

                                                               tCGTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCg         >  1:2293831/1‑76 (MQ=255)

                                                                 gTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGaa       <  2:1827781/76‑1 (MQ=255)

                                                                 gTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGaa       >  2:634296/1‑76 (MQ=255)

                                                                 gTTATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGaa       >  2:1529651/1‑76 (MQ=255)

                                                                  ttATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAAc      >  1:1872826/1‑76 (MQ=255)

                                                                  ttATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAAc      >  1:1048068/1‑76 (MQ=255)

                                                                   tATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACt     <  1:2422855/76‑1 (MQ=255)

                                                                   tATGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACt     >  1:1847786/1‑76 (MQ=255)

                                                                     tGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTgg   <  2:2260182/76‑1 (MQ=255)

                                                                     tGGAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTgg   >  2:416673/1‑76 (MQ=255)

                                                                      ggAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGc  >  2:4753268/1‑76 (MQ=255)

                                                                      ggAGGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGc  <  2:2383027/76‑1 (MQ=255)

CAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGC  >  REL606/782465‑782610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`