BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	805,738	C→T	intergenic (+165/‑414)	bioD → / → uvrB	dethiobiotin synthetase/excinuclease ABC subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	805,738	0	C	T	100.0%	415.3 / NA	107	intergenic (+165/‑414)	bioD/uvrB	dethiobiotin synthetase/excinuclease ABC subunit B
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (54/53); total (54/53)

Only 100 of 120 total aligned reads displayed.

TTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATACGCGGCTTGCA  >  REL606/805665‑805810

ttttACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACCGTTATCTAc                                                                        >  1:1655391/1‑76 (MQ=255)

ttttACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTAc                                                                        >  1:1586390/1‑76 (MQ=255)

 tttACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACt                                                                       <  1:5187398/76‑1 (MQ=255)

 tttACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACt                                                                       <  2:1120724/76‑1 (MQ=255)

  ttACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATctacta                                                                      >  2:3441453/1‑76 (MQ=255)

  ttACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATctacta                                                                      >  2:179464/1‑76 (MQ=255)

  ttACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATctacta                                                                      >  2:4870364/1‑76 (MQ=255)

  ttACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATctacta                                                                      >  2:837491/1‑76 (MQ=255)

   tACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTAt                                                                     <  1:376237/76‑1 (MQ=255)

   tACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTAt                                                                     <  1:5605521/76‑1 (MQ=255)

    tcTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTAtt                                                                    <  2:1198404/75‑1 (MQ=255)

     cTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTc                                                                   <  2:2347430/76‑1 (MQ=255)

      ttCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTcc                                                                  >  1:187655/1‑76 (MQ=255)

      ttCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTcc                                                                  >  1:186605/1‑76 (MQ=255)

      ttCCGCCTCATGCGGCGAATGGGGGAATTGCCCAGGCGGCGGGGGTTAGGGGCTGGAGGCAGCTATATACTATTcc                                                                  >  2:1529720/1‑76 (MQ=255)

        ccGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGGGACAGTTATCTACTATTTCtg                                                                >  1:2018316/1‑76 (MQ=255)

        ccGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCtg                                                                >  1:2710311/1‑76 (MQ=255)

        ccGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCtg                                                                <  2:5222300/76‑1 (MQ=255)

        ccGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCtg                                                                <  2:4837708/76‑1 (MQ=255)

        ccGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGAAAGTTATCTACTATTCCtg                                                                >  1:4093278/1‑76 (MQ=255)

          gCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCtgtg                                                              >  1:5974310/1‑76 (MQ=255)

          gCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGAAAGTTATCTACTATTCCtgtg                                                              >  2:4597517/1‑76 (MQ=255)

           ccTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTAACTTCTATTTCTGTgg                                                             >  1:5475267/1‑76 (MQ=255)

            cTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGGTATCTACTATTCCTGTGGa                                                            >  2:4429987/1‑76 (MQ=255)

               aTGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATaa                                                         <  1:398340/76‑1 (MQ=255)

               aTGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATaa                                                         <  1:6188721/76‑1 (MQ=255)

                tGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAAc                                                        <  1:991105/76‑1 (MQ=255)

                 gcggcgAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAAcc                                                       <  1:2570515/76‑1 (MQ=255)

                  cggcgAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCa                                                      <  2:2409209/76‑1 (MQ=255)

                  cggcgAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCa                                                      <  1:5083336/76‑1 (MQ=255)

                  cggcgAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCa                                                      >  1:5541173/1‑76 (MQ=255)

                  cggcgAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCa                                                      <  2:5462891/76‑1 (MQ=255)

                     cgAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCAtgt                                                   >  2:1904479/1‑76 (MQ=255)

                     cgAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAAACAtgt                                                   >  1:5095333/1‑76 (MQ=255)

                      gAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCAtgtg                                                  <  2:1413203/76‑1 (MQ=255)

                       aaTGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATgtgt                                                 >  2:1527815/1‑76 (MQ=255)

                        aTGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTa                                                <  1:5380011/76‑1 (MQ=255)

                          gtgGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTTCTATTCCTGTGGATAACCATGTGTAtt                                              <  1:6314010/76‑1 (MQ=255)

                          gggggAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTAtt                                              <  1:439955/74‑1 (MQ=255)

                           tgGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTa                                             >  2:2663763/1‑76 (MQ=255)

                            gggAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTag                                            >  2:3279264/1‑76 (MQ=255)

                             ggAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTaga                                           <  1:2927267/76‑1 (MQ=255)

                              gAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTagag                                          <  2:2082937/76‑1 (MQ=255)

                              gAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTagag                                          <  1:4261593/76‑1 (MQ=255)

                              gAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTagag                                          <  2:5873777/76‑1 (MQ=255)

                               aaTTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGt                                         <  2:6225724/76‑1 (MQ=255)

                                aTTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGtt                                        >  1:1458237/1‑76 (MQ=255)

                                aTTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGGATTAGAGtt                                        >  2:4182644/1‑76 (MQ=255)

                                 ttGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTa                                       >  2:5282401/1‑76 (MQ=255)

                                  tGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAg                                      <  2:2424175/76‑1 (MQ=255)

                                  tGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAg                                      <  2:3110896/76‑1 (MQ=255)

                                   gCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGa                                     >  1:3717743/1‑76 (MQ=255)

                                     ccAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGaaa                                   <  2:2850971/76‑1 (MQ=255)

                                     ccAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGaaa                                   <  1:3851044/76‑1 (MQ=255)

                                      cAGGCGGCGGGGGGTAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGaaaa                                  <  1:1005468/76‑1 (MQ=255)

                                      cAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGaaaa                                  >  2:2610678/1‑76 (MQ=255)

                                      cAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGaaaa                                  <  2:85407/76‑1 (MQ=255)

                                      cAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGaaaa                                  >  1:5522732/1‑76 (MQ=255)

                                       aGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGaaaaa                                 >  1:5917255/1‑75 (MQ=255)

                                       aGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAac                                 >  2:1417491/1‑76 (MQ=255)

                                        ggcggcGGGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAaca                                <  2:1589856/76‑1 (MQ=255)

                                          cggcggGGGATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACg                              >  2:5230215/1‑76 (MQ=255)

                                              gggggATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGc                          >  1:3710110/1‑76 (MQ=255)

                                              gggggATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGc                          >  1:4229042/1‑76 (MQ=255)

                                               ggggATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCa                         <  1:905910/76‑1 (MQ=255)

                                               ggggATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCa                         >  2:663388/1‑76 (MQ=255)

                                               ggggATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCa                         <  1:659043/76‑1 (MQ=255)

                                               ggggATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCa                         <  1:5967097/76‑1 (MQ=255)

                                               ggggATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCa                         <  1:273112/76‑1 (MQ=255)

                                                gggATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCaa                        <  2:2505491/76‑1 (MQ=255)

                                                gggATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCaa                        <  2:3275549/76‑1 (MQ=255)

                                                 ggATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAg                       >  1:6308617/1‑76 (MQ=255)

                                                  gATAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGc                      >  1:1672271/1‑76 (MQ=255)

                                                   aTAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCg                     >  1:3451127/1‑76 (MQ=255)

                                                    tAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCga                    >  2:4787983/1‑76 (MQ=255)

                                                    tAGGGGCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCga                    >  2:4665238/1‑76 (MQ=255)

                                                       gggCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCgagag                 >  1:2479056/1‑76 (MQ=255)

                                                       gggCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCgagag                 <  2:2438069/76‑1 (MQ=255)

                                                       gggCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCgagag                 <  1:99705/76‑1 (MQ=255)

                                                       gggCTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCgagag                 >  1:3599944/1‑76 (MQ=255)

                                                          cTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAAt              >  2:2901310/1‑76 (MQ=255)

                                                          cTGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAAt              <  1:532258/76‑1 (MQ=255)

                                                           tGGAGACAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGAAAGCGAGAGAATa             <  1:4215529/76‑1 (MQ=255)

                                                             gagaCAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATAcg           >  1:3327116/1‑76 (MQ=255)

                                                             gagaCAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATAcg           <  2:5081679/76‑1 (MQ=255)

                                                              agaCAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATAcgc          >  2:3897510/1‑76 (MQ=255)

                                                               gaCAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATAcgcg         <  2:4930200/76‑1 (MQ=255)

                                                                acacTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATACGCgg        >  2:104115/1‑76 (MQ=255)

                                                                aCAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATACGCgg        >  1:1185316/1‑76 (MQ=255)

                                                                 cAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATACGCGGc       >  2:1954027/1‑76 (MQ=255)

                                                                 cAGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAAAACGCGGc       >  2:1925942/1‑76 (MQ=255)

                                                                  aGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATACGCGGCt      <  2:3413903/76‑1 (MQ=255)

                                                                  aGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATACGCGGCt      <  1:299963/76‑1 (MQ=255)

                                                                  aGTTATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATACGCGGCt      <  1:4821198/76‑1 (MQ=255)

                                                                   gTTATCTACTATGCCTGTTGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATACGCGGCtt     <  1:1471469/76‑1 (MQ=255)

                                                                    ttATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATACGCGGCTTg    >  1:557882/1‑76 (MQ=255)

                                                                     tATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATACGCGGCTTGc   >  1:4299182/1‑76 (MQ=255)

                                                                     tATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATACGCGGCTTGc   <  1:2610678/76‑1 (MQ=255)

                                                                     tATCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATACGCGGCTTGc   <  2:2923178/76‑1 (MQ=255)

                                                                      aTCTACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATACGCGGCTTGCa  <  2:4304267/76‑1 (MQ=255)

TTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATACGCGGCTTGCA  >  REL606/805665‑805810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`