BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	852,548	G→A	D11D (GAC→GAT)	ybiV ←	predicted hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	852,548	0	G	A	99.0%	829.6 / ‑2.0	202	D11D (GAC→GAT)	ybiV	predicted hydrolase
Reads supporting (aligned to +/- strand): ref base G (2/0); new base A (106/94); total (108/94)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 207 total aligned reads displayed.

GCTTTTTCAGTTCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAGTGTA  >  REL606/852475‑852620

 cTTTTTCAGTTCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATcc                                                                       >  2:1972299/1‑76 (MQ=255)

 cTTTTTCAGTTCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATcc                                                                       >  2:2352116/1‑76 (MQ=255)

 cTTTTTCAGTTCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATcc                                                                       <  1:2726214/76‑1 (MQ=255)

  tttttCAGTTCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCa                                                                      >  1:4312846/1‑76 (MQ=255)

  tttttCAGTTCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCa                                                                      >  1:3808061/1‑76 (MQ=255)

   ttttCAGTTCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTAccatccat                                                                     <  2:950834/76‑1 (MQ=255)

   ttttCAGTTCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTAccatccat                                                                     <  1:762743/76‑1 (MQ=255)

   ttttCAGTTCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTAccatccat                                                                     <  1:979764/76‑1 (MQ=255)

   ttttCAGTTCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTAccatccat                                                                     >  2:5190068/1‑76 (MQ=255)

    tttCAGTTCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATg                                                                    <  2:4158297/76‑1 (MQ=255)

    tttCAGTTCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATg                                                                    <  2:1696271/76‑1 (MQ=255)

    tttCAGTTCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATg                                                                    >  2:316526/1‑76 (MQ=255)

       cAGTTCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTCCCATCCATGTCt                                                                 <  2:4491512/76‑1 (MQ=255)

       cAGTTCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCt                                                                 <  1:1423974/76‑1 (MQ=255)

       cAGTTCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCt                                                                 >  2:4450022/1‑76 (MQ=255)

       cAGTTCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCt                                                                 >  1:620296/1‑76 (MQ=255)

       cAGTTCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCt                                                                 <  1:1285127/76‑1 (MQ=255)

        aGTTCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCtg                                                                >  1:329528/1‑76 (MQ=255)

        aGTTCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCtg                                                                <  1:5729492/76‑1 (MQ=255)

          ttCCTGATCGTGGGCTATAAAACGTGGTTGGTTGTACGTGTTGTCGTCGTTAACAAAAGTGCCATCCATGTCtgtg                                                              <  2:3849145/76‑1 (MQ=255)

          ttCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTCCCATCCATGTCtgtg                                                              <  1:1373576/76‑1 (MQ=255)

          ttCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCtgtg                                                              >  1:5460909/1‑76 (MQ=255)

          ttCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCtgtg                                                              >  1:1202890/1‑76 (MQ=255)

          ttCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCtgtg                                                              <  2:4293464/76‑1 (MQ=255)

            ccTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGAc                                                            <  2:1161386/76‑1 (MQ=255)

             cTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACg                                                           <  2:5456892/76‑1 (MQ=255)

              tGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGa                                                          >  1:5247759/1‑76 (MQ=255)

              tGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGa                                                          >  2:3436386/1‑76 (MQ=255)

               gATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGAt                                                         >  2:1512371/1‑76 (MQ=255)

                atatTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATa                                                        >  1:1631414/1‑76 (MQ=255)

                atatTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATa                                                        >  1:637648/1‑76 (MQ=255)

                  atTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAAc                                                      <  1:4915649/76‑1 (MQ=255)

                  atTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAAc                                                      <  2:6129688/76‑1 (MQ=255)

                  atTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAAc                                                      >  1:3571308/1‑76 (MQ=255)

                   ttGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACt                                                     >  2:5391494/1‑76 (MQ=255)

                     gcgcCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACttt                                                   >  2:3023584/1‑76 (MQ=255)

                      cgcCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACtttt                                                  >  2:1379080/1‑76 (MQ=255)

                      cgcCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACtttt                                                  >  1:628861/1‑76 (MQ=255)

                      cgcCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACtttt                                                  >  1:3860094/1‑76 (MQ=255)

                      cgcCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACtttt                                                  <  1:2866498/76‑1 (MQ=255)

                       gcCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTa                                                 >  2:4061737/1‑76 (MQ=255)

                       gcCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTa                                                 <  2:1469572/76‑1 (MQ=255)

                        ccATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTAc                                                <  2:619971/76‑1 (MQ=255)

                        ccATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTAc                                                >  2:5881644/1‑76 (MQ=255)

                        ccATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTAc                                                >  2:1583299/1‑76 (MQ=255)

                         cATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACg                                               <  2:2209259/76‑1 (MQ=255)

                            aaaaCGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTc                                            >  1:5895597/1‑76 (MQ=255)

                              aaCGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCAt                                          >  2:757719/1‑76 (MQ=255)

                              aaCGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCAt                                          <  1:4013154/76‑1 (MQ=255)

                               aCGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATa                                         <  2:5740902/76‑1 (MQ=255)

                               aCGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATa                                         >  1:6295394/1‑76 (MQ=255)

                                cGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAg                                        >  1:1058427/1‑76 (MQ=255)

                                        gttgTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTc                                >  1:224151/1‑76 (MQ=255)

                                        gttgTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTc                                >  1:140028/1‑76 (MQ=255)

                                         ttgTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTcc                               >  1:5644948/1‑76 (MQ=255)

                                          tgTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCt                              <  2:656230/76‑1 (MQ=255)

                                          tgTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCt                              >  1:132941/1‑76 (MQ=255)

                                          tgTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCt                              >  1:2124983/1‑76 (MQ=255)

                                          tgTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCt                              <  1:1051239/76‑1 (MQ=255)

                                           gTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTg                             <  2:244686/76‑1 (MQ=255)

                                           gTACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTg                             <  2:5174043/76‑1 (MQ=255)

                                           gTACGTTTTGCCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTg                             <  1:2516275/76‑1 (MQ=255)

                                            tACGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTgg                            <  1:867690/76‑1 (MQ=255)

                                             aCGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGc                           >  1:4167053/1‑76 (MQ=255)

                                             aCGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGc                           >  2:3875977/1‑76 (MQ=255)

                                             aCGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGc                           <  2:2747732/76‑1 (MQ=255)

                                              cGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCt                          <  1:1926885/76‑1 (MQ=255)

                                              cGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCt                          >  1:2017053/1‑76 (MQ=255)

                                              cGTTTTGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCt                          <  2:907738/76‑1 (MQ=255)

                                                ttttGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCg                        >  2:4349438/1‑76 (MQ=255)

                                                 tttGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTcca                       >  1:2789786/1‑74 (MQ=255)

                                                   tGGCGTCGTTAAGAAAAGTCCCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGaaa                     <  1:3908929/76‑1 (MQ=255)

                                                   tGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGTTCATAGGTGTGCTCCTGGCTCGgaa                     >  1:4735935/1‑76 (MQ=255)

                                                   tGGCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGaaa                     >  1:5082352/1‑76 (MQ=255)

                                                    ggCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGaaaa                    >  1:839815/1‑76 (MQ=255)

                                                     gCGTCGTTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAAt                   <  1:3553226/76‑1 (MQ=255)

                                                      cgtcgtTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAAta                  >  2:124064/1‑75 (MQ=255)

                                                      cgtcgtTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATg                  <  2:2011244/76‑1 (MQ=255)

                                                        tcgtTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGaa                <  1:3786362/76‑1 (MQ=255)

                                                         cgtTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGaaa               <  1:103716/76‑1 (MQ=255)

                                                         cgtTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGaaa               <  1:4623572/76‑1 (MQ=255)

                                                          gtTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAAc              <  2:5643655/76‑1 (MQ=255)

                                                          gtTAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAAc              <  1:5951860/76‑1 (MQ=255)

                                                           ttAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATTAAAcc             >  1:3039758/1‑76 (MQ=255)

                                                           ttAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAAcc             >  2:3521012/1‑76 (MQ=255)

                                                            tAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCg            >  1:100397/1‑76 (MQ=255)

                                                            tAAGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCg            >  1:6257953/1‑76 (MQ=255)

                                                             aaGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGt           <  2:4804897/76‑1 (MQ=255)

                                                              aGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGCAAATGAAACCGTa          >  1:1859186/1‑76 (MQ=255)

                                                              aGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTa          <  1:2454111/76‑1 (MQ=255)

                                                              aGAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTa          <  2:5068918/76‑1 (MQ=255)

                                                               gAAAAGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTaa         >  2:1138578/1‑76 (MQ=255)

                                                                  aaGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAg      >  1:1478095/1‑76 (MQ=255)

                                                                   aGTCCCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAgt     <  1:39697/76‑1 (MQ=255)

                                                                   aGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAgt     <  1:4053609/76‑1 (MQ=255)

                                                                   aGTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCCTAACAgt     >  2:3357433/1‑76 (MQ=255)

                                                                    gTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAgtg    >  2:2560503/1‑76 (MQ=255)

                                                                    gTACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCCCAAATGAAAACGTAACAgtg    >  1:4767879/1‑76 (MQ=255)

                                                                     tACCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAgtgt   >  1:4535681/1‑76 (MQ=255)

                                                                      aCCATCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAGTGta  >  1:4380079/1‑76 (MQ=255)

GCTTTTTCAGTTCCTGATATTGCGCCATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAGTGTA  >  REL606/852475‑852620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`