| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,030,403 | C→T | P18P (CCC→CCT) | pqiB → | paraquat‑inducible protein B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,030,403 | 0 | C | T | 98.8% | 659.1 / ‑2.4 | 162 | P18P (CCC→CCT) | pqiB | paraquat‑inducible protein B |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (79/81); total (79/83) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
Only 100 of 170 total aligned reads displayed.
GCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCAGGGACCG > REL606/1030330‑1030478 | gCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTgt < 1:5420809/76‑1 (MQ=255)gCAGGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTgt < 2:5646394/76‑1 (MQ=255) cATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCtgtg < 1:2374957/76‑1 (MQ=255) cATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCtgtg > 1:5661293/1‑76 (MQ=255) aTGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTgtgt < 2:6259601/76‑1 (MQ=255) aTGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTgtgt < 1:1206317/76‑1 (MQ=255) aTGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTgtgt < 1:2147291/76‑1 (MQ=255) aTGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTgtgt > 1:827323/1‑76 (MQ=255) aTGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTgtgt > 2:2916698/1‑76 (MQ=255) tGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCtgtgtg > 1:1504893/1‑76 (MQ=255) gaggagTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTgg > 2:1970873/1‑76 (MQ=255) gaggagTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTgg < 2:2339969/76‑1 (MQ=255) ggagTCCTGACAGTAGGGAATCTAATAAGGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGat < 1:4344352/76‑1 (MQ=255) gattCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGata < 2:1058625/73‑1 (MQ=255) agTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGatat < 2:2270404/76‑1 (MQ=255) agTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGatat < 2:3132473/76‑1 (MQ=255) gTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATAtt < 1:1412897/76‑1 (MQ=255) tCCTGACAGTATGGAATCTAATAATGGGGAAGTCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATAttt < 2:1883439/76‑1 (MQ=255) tCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATAttt < 2:3626013/76‑1 (MQ=255) tCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATAttt < 2:6006060/76‑1 (MQ=255) tCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATAttt > 1:1314848/1‑76 (MQ=255) ccTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTc > 2:3218816/1‑76 (MQ=255) cTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTcc > 1:2364518/1‑76 (MQ=255) tGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCt < 1:4177446/76‑1 (MQ=255) gACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTa < 2:2501428/76‑1 (MQ=255) gACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTa < 2:4108940/76‑1 (MQ=255) cAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATc > 2:830060/1‑76 (MQ=255) cAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATc < 1:70699/76‑1 (MQ=255) cAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATc < 2:2145509/76‑1 (MQ=255) cAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATc < 1:6186004/76‑1 (MQ=255) aGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCg > 1:408907/1‑76 (MQ=255) aGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCg < 2:1703439/76‑1 (MQ=255) gTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGt < 1:1269562/76‑1 (MQ=255) tATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTc > 2:4375278/1‑76 (MQ=255) ggAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACg > 2:1252078/1‑76 (MQ=255) ggAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACg < 1:107093/76‑1 (MQ=255) ggAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACg < 1:4707091/76‑1 (MQ=255) ggAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACg < 1:436465/76‑1 (MQ=255) gAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACgg > 2:3324494/1‑76 (MQ=255) aaTCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGgc > 1:1004051/1‑76 (MQ=255) cTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCt > 1:4905397/1‑76 (MQ=255) cTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCt > 1:2031179/1‑76 (MQ=255) cTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCt > 1:3144950/1‑76 (MQ=255) aataatGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCa > 2:5703304/1‑76 (MQ=255) aataatGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCa > 1:5319665/1‑76 (MQ=255) aataatGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCa < 2:5626448/76‑1 (MQ=255) aataatGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCa > 1:2977815/1‑76 (MQ=255) aataatGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCa > 1:592182/1‑76 (MQ=255) ataatGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCAt > 2:2807544/1‑76 (MQ=255) ataatGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCAt < 2:3795538/76‑1 (MQ=255) ataatGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCAt > 1:466034/1‑76 (MQ=255) taatGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCAtt < 1:6123920/76‑1 (MQ=255) taatGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCAtt < 2:3991983/76‑1 (MQ=255) aaatGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCAtt < 1:3227936/75‑1 (MQ=255) aatGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTg > 1:4572472/1‑76 (MQ=255) aatGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTg < 1:2821692/76‑1 (MQ=255) atGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTgg > 1:4140555/1‑76 (MQ=255) atGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTgg > 1:937228/1‑76 (MQ=255) tGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTggg > 2:4844303/1‑76 (MQ=255) gAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCt < 1:6204960/76‑1 (MQ=255) gAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCt < 1:3470785/76‑1 (MQ=255) aaGCCAAAATCCAGAAAGTGAATAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTg < 2:1698143/76‑1 (MQ=255) ccAAAATCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGt > 2:3593024/1‑76 (MQ=255) aaaaTCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTc < 2:5012097/76‑1 (MQ=255) aaaTCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCt < 1:4806386/76‑1 (MQ=255) aaaTCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCt < 1:4067926/76‑1 (MQ=255) aaaTCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCt < 1:395709/76‑1 (MQ=255) aaTCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCtt < 1:4683664/76‑1 (MQ=255) aTCCAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCttt > 2:5974880/1‑76 (MQ=255) ccAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCttttt > 1:3269417/1‑76 (MQ=255) ccAGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCttttt > 2:5879514/1‑76 (MQ=255) aGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTa > 2:4326129/1‑76 (MQ=255) aGAAAGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTa > 1:381311/1‑76 (MQ=255) aaaGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATc < 1:920838/76‑1 (MQ=255) aaGTGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCa > 1:5868584/1‑76 (MQ=255) tGAAGAACTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTa < 1:3505195/76‑1 (MQ=255) gaagaaCTGGTCTCCTGTGTGGATGTTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTAc > 1:4518153/1‑76 (MQ=255) gaagaaCTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTAc > 1:1363505/1‑76 (MQ=255) gaagaaCTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTAc > 2:1115330/1‑76 (MQ=255) gaagaaCTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTAc > 2:5486069/1‑76 (MQ=255) gagaaCTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACa < 2:1379217/75‑1 (MQ=255) aagaaCTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACa < 2:4518221/76‑1 (MQ=255) agaaCTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAg < 1:5213055/76‑1 (MQ=255) gaaCTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGc < 2:4990419/76‑1 (MQ=255) gaaCTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGc < 2:1106975/76‑1 (MQ=255) aCTGGTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCa > 1:5899114/1‑76 (MQ=255) ggTCTCCTGTGTGGATTTTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCa < 1:1947276/76‑1 (MQ=255) gTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCAg > 2:521244/1‑76 (MQ=255) gTCTCCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCAg > 1:5798760/1‑76 (MQ=255) tctcCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCAgg < 1:1657663/76‑1 (MQ=255) tctcCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCAgg < 2:2448593/76‑1 (MQ=255) tctcCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCAgg > 2:3330212/1‑76 (MQ=255) ctcCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCAggg < 1:3274558/76‑1 (MQ=255) ctcCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCAggg > 2:418571/1‑76 (MQ=255) ctcCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCAggg > 2:5885637/1‑76 (MQ=255) ctcCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCAggg < 2:5983033/76‑1 (MQ=255) ctcCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCAggg > 1:1433015/1‑76 (MQ=255) tcCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCAGGGa < 1:2702129/76‑1 (MQ=255) tcCTGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCAGGGa > 2:3279930/1‑76 (MQ=255) cGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCAGGGACCg > 1:4441794/1‑76 (MQ=255) | GCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAGAACTGGTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCAGGGACCG > REL606/1030330‑1030478 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |