| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,198,004 | A→G | Y66H (TAT→CAT) | potD ← | spermidine/putrescine ABC transporter periplasmic substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,198,004 | 0 | A | G | 99.4% | 723.9 / ‑4.9 | 172 | Y66H (TAT→CAT) | potD | spermidine/putrescine ABC transporter periplasmic substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (95/76); total (95/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
Only 100 of 189 total aligned reads displayed.
ATTTTATCGACGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAG > REL606/1197931‑1198076 | aTTTTATCGACGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCa > 1:6211783/1‑76 (MQ=255)aTTTTATCGACGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCa > 1:5313608/1‑76 (MQ=255) ttttATCGACGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGgcatgcat < 2:4143048/76‑1 (MQ=255) ttttATCGACGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGgcatgcat > 2:1829645/1‑76 (MQ=255) ttttATCGACGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGgcatgcat > 1:2802192/1‑76 (MQ=255) ttttATCGACGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGgcatgcat < 2:6012288/76‑1 (MQ=255) tttATCGACGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGcatgcatg > 1:4906635/1‑76 (MQ=255) ttATCGACGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATgg > 1:4960357/1‑76 (MQ=255) ttATCGACGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATgg > 1:6289696/1‑76 (MQ=255) ttATCGACGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATgg > 1:2752968/1‑76 (MQ=255) tATCGACGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGCCATGCATGGt > 1:4157199/1‑76 (MQ=255) aTCGACGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGtt > 1:58797/1‑76 (MQ=255) tCGACGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGttt < 1:2246479/76‑1 (MQ=255) tCGACGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGttt > 1:4709694/1‑76 (MQ=255) tCGACGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGttt < 2:2099826/76‑1 (MQ=255) tCGACGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGttt < 1:1100820/76‑1 (MQ=255) tCGACGTAATAGGTTGAAGGAACCACCAGGCCATAGGCACCGTCTTTGTACGTTTTTAGCTTGGAATGATTGGttt > 1:3532502/1‑76 (MQ=255) cGACGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCTGCTTGGCATGCATGGTttc < 2:4848323/76‑1 (MQ=255) cGACGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTttc > 1:395340/1‑76 (MQ=255) gACGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTttcg < 1:2100502/76‑1 (MQ=255) gACGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTttcg > 1:5640260/1‑76 (MQ=255) cGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTttcgtt < 1:3807/76‑1 (MQ=255) cGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTttcgtt > 2:4320847/1‑76 (MQ=255) cGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTttcgtt < 1:99128/76‑1 (MQ=255) cGTAAGAGTTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTttcgtt < 2:5606231/76‑1 (MQ=255) gTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTttcgtcg > 1:1576727/1‑74 (MQ=255) tAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTttcgttcg > 1:3201975/1‑76 (MQ=255) tAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTttcgttcg > 2:3872046/1‑76 (MQ=255) tAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTttcgttcg > 2:5671179/1‑76 (MQ=255) tAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTttcgttcg < 2:75281/76‑1 (MQ=255) aaTAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGa > 1:5686142/1‑76 (MQ=255) tAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACt > 2:2219277/1‑76 (MQ=255) aGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTc > 2:5602517/1‑76 (MQ=255) aGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTc > 2:5068685/1‑76 (MQ=255) aGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTc > 1:2488692/1‑76 (MQ=255) aGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTc > 1:5267204/1‑76 (MQ=255) aGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTc < 2:2811640/76‑1 (MQ=255) aGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTc > 1:1458847/1‑76 (MQ=255) ggTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCg < 2:4467854/76‑1 (MQ=255) gTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGt < 2:859348/76‑1 (MQ=255) ttGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTa > 1:733458/1‑76 (MQ=255) tGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTaa > 1:951225/1‑76 (MQ=255) gAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAg < 2:5309020/76‑1 (MQ=255) gAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAg < 1:3445090/76‑1 (MQ=255) aaGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGt > 2:5161243/1‑76 (MQ=255) aaGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGt > 2:2852755/1‑76 (MQ=255) aGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTc > 2:2748909/1‑76 (MQ=255) aGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTc > 1:5629672/1‑76 (MQ=255) aGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTc < 1:3160214/76‑1 (MQ=255) gAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGa > 2:1561019/1‑76 (MQ=255) ccaccaGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATa < 2:3600547/76‑1 (MQ=255) caccaGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAg < 1:5130327/76‑1 (MQ=255) caccaGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAg < 2:1364527/76‑1 (MQ=255) ccaGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGAt > 2:2409359/1‑76 (MQ=255) caGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATa > 2:2617563/1‑76 (MQ=255) caGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATa < 1:1292776/76‑1 (MQ=255) caGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATa < 1:4452006/76‑1 (MQ=255) ggTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAAc > 1:1728059/1‑76 (MQ=255) cATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCtt < 1:5149286/76‑1 (MQ=255) aTAGGCATCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTa < 2:268521/76‑1 (MQ=255) aTAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTa > 2:3790933/1‑76 (MQ=255) aTAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTa > 2:1052617/1‑76 (MQ=255) aTAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTa > 1:5750358/1‑76 (MQ=255) aGGCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAAt < 2:1000335/76‑1 (MQ=255) aGGCACCGGCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAAt < 2:5762498/76‑1 (MQ=255) ggCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATa < 1:2305221/76‑1 (MQ=255) ggCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATa < 1:2397196/76‑1 (MQ=255) gCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATAc < 1:4203534/76‑1 (MQ=255) gCACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATAc < 1:3836775/76‑1 (MQ=255) cACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATAcc > 1:1160146/1‑76 (MQ=255) cACCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATAcc > 2:1661401/1‑76 (MQ=255) aCCGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCg < 1:1955279/76‑1 (MQ=255) ccGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCgg < 2:1250801/76‑1 (MQ=255) cGTCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGt > 2:3511440/1‑76 (MQ=255) tCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGttt > 1:501196/1‑76 (MQ=255) tCTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGttt > 1:3628507/1‑76 (MQ=255) cTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTc > 1:907147/1‑76 (MQ=255) cTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTc > 1:2861887/1‑76 (MQ=255) cTTTGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTc > 1:343004/1‑76 (MQ=255) tttGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCt > 2:1523937/1‑76 (MQ=255) tttGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCt > 1:3013741/1‑76 (MQ=255) tttGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCt > 2:5408036/1‑76 (MQ=255) tGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCttt > 1:4836968/1‑76 (MQ=255) tGTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCttt < 1:4541193/76‑1 (MQ=255) gTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTg < 1:4006107/76‑1 (MQ=255) gTACGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTg < 1:5561687/76‑1 (MQ=255) aCGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGt < 2:4354399/76‑1 (MQ=255) aCGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGt > 1:5919069/1‑76 (MQ=255) cGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTg > 2:1756146/1‑76 (MQ=255) cGTTTTCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTg < 2:2817385/76‑1 (MQ=255) ttCAGCTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACt < 2:6226948/76‑1 (MQ=255) cTTGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCa > 1:2760465/1‑76 (MQ=255) ttGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCaa > 1:390229/1‑76 (MQ=255) ttGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCaa < 2:6211783/76‑1 (MQ=255) tGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAg > 1:5048547/1‑76 (MQ=255) tGGCATGCATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAg > 2:609898/1‑76 (MQ=255) gcatgcatGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCa < 2:4057402/76‑1 (MQ=255) gcatgcatGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCa > 1:3257425/1‑76 (MQ=255) gcatgcatGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCa > 2:58710/1‑76 (MQ=255) catgcatgGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAg > 1:1376230/1‑76 (MQ=255) | ATTTTATCGACGTAATAGGTTGAAGGAACCACCAGGTCATAGGCACCGTCTTTGTACGTTTTCAGCTTGGCATACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAG > REL606/1197931‑1198076 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |