BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,282,843	C→T	P944S (CCG→TCG)	narG →	nitrate reductase 1, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,282,843	0	C	T	100.0%	564.8 / NA	146	P944S (CCG→TCG)	narG	nitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (68/78); total (68/78)

Only 100 of 154 total aligned reads displayed.

GAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAG  >  REL606/1282770‑1282915

gtacacCCAGAGCGAGATGGATCTGCTGCGTAAGCACAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCg                                                                        <  2:5808145/74‑1 (MQ=255)

gAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCg                                                                        <  2:1491194/76‑1 (MQ=255)

gAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCg                                                                        <  2:3784733/76‑1 (MQ=255)

 aaCACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGa                                                                       >  1:3302041/1‑76 (MQ=255)

  acacCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGAt                                                                      <  2:3957299/76‑1 (MQ=255)

   cgcccAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATg                                                                     <  1:2107058/74‑1 (MQ=255)

   cacCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATg                                                                     <  1:3803206/76‑1 (MQ=255)

    acCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGGCAGTCGATGc                                                                    >  2:4927882/1‑76 (MQ=255)

     cccAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCt                                                                   <  1:5381155/76‑1 (MQ=255)

      ccAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGGTg                                                                  >  1:1013867/1‑76 (MQ=255)

      ccAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTg                                                                  >  1:4135580/1‑76 (MQ=255)

      ccAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTg                                                                  <  2:2270374/76‑1 (MQ=255)

      ccAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTg                                                                  <  1:2624215/76‑1 (MQ=255)

       cAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACCACAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGa                                                                 <  1:4050491/76‑1 (MQ=255)

       cAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGa                                                                 >  1:3661430/1‑76 (MQ=255)

        agagCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCTATGCTGaa                                                                >  1:2186942/1‑76 (MQ=255)

        agagCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGGCCGGCGAAAGGCCAGTCGATGCTGaa                                                                >  2:5388690/1‑76 (MQ=255)

           gCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAacac                                                             <  2:6257271/76‑1 (MQ=255)

           gCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAacac                                                             <  2:1962136/76‑1 (MQ=255)

            cGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAacaac                                                            >  2:5873137/1‑74 (MQ=255)

            cGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACAcc                                                            <  1:1858609/76‑1 (MQ=255)

            cGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACAcc                                                            <  1:5157015/76‑1 (MQ=255)

             gagaTGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCg                                                           <  2:1124267/76‑1 (MQ=255)

             gagaTGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCg                                                           >  2:5229262/1‑76 (MQ=255)

             gagaTGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCg                                                           >  1:2208935/1‑76 (MQ=255)

                 tGGATCTGCTGCGTGAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCCAt                                                       >  1:3252232/1‑76 (MQ=255)

                 tGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAAt                                                       <  2:4817692/76‑1 (MQ=255)

                  ggATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAAtt                                                      >  2:1084400/1‑76 (MQ=255)

                   gATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTg                                                     >  1:2365684/1‑76 (MQ=255)

                    aTCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGa                                                    >  1:5665384/1‑76 (MQ=255)

                    aTCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGa                                                    >  2:5738073/1‑76 (MQ=255)

                      ctgctgCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATg                                                  <  1:5869039/76‑1 (MQ=255)

                      ctgctgCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATg                                                  >  1:193892/1‑76 (MQ=255)

                      ctgctgCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATg                                                  <  2:1493165/76‑1 (MQ=255)

                        gctgcGTAAGCTCAACTACACCAAAGCGGAAGGTCTGGCGAAAGGCCAGTCGATGCGGTACACAGGAATTGATGCg                                                >  1:2713751/1‑76 (MQ=255)

                         ctgcGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCgg                                               >  2:2430616/1‑76 (MQ=255)

                         ctgcGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCgg                                               <  1:294345/76‑1 (MQ=255)

                         ctgcGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCgg                                               <  2:3395639/76‑1 (MQ=255)

                            cGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCag                                            <  1:3147990/76‑1 (MQ=255)

                              tAAGCTCAACTACACCAAAGCGTAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCagag                                          <  1:1316644/76‑1 (MQ=255)

                              tAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCagag                                          <  1:890875/76‑1 (MQ=255)

                              tAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCagag                                          <  1:306177/76‑1 (MQ=255)

                              tAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCagag                                          >  2:3520689/1‑76 (MQ=255)

                               aaGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACAcc                                                            >  2:3816611/1‑57 (MQ=255)

                               aaGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACAcc                                                            <  1:3816611/57‑1 (MQ=255)

                               aaGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAgaga                                         >  2:1672976/1‑76 (MQ=255)

                               aaGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAgaga                                         <  2:1908437/76‑1 (MQ=255)

                                aGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAgat                                        <  1:4350405/76‑1 (MQ=255)

                                aGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAgat                                        <  1:1339885/76‑1 (MQ=255)

                                aGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAgat                                        <  2:4481307/76‑1 (MQ=255)

                                  cTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATACGGCAGAgatga                                      <  2:4550148/76‑1 (MQ=255)

                                   tCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAgatgat                                     <  2:5589341/76‑1 (MQ=255)

                                   tCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAgatga                                      <  2:477671/75‑1 (MQ=255)

                                   tCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAAAACCGCAATTGATGCGGTAGAggtga                                      >  1:477671/1‑75 (MQ=255)

                                     aaCTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATcc                                   >  1:4855862/1‑76 (MQ=255)

                                     aaCTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATcc                                   >  2:2065658/1‑76 (MQ=255)

                                      aCTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCt                                  >  2:2726345/1‑76 (MQ=255)

                                      aCTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCt                                  <  1:4727924/76‑1 (MQ=255)

                                      aCTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGAAGATCCt                                  >  1:2923157/1‑76 (MQ=255)

                                      aCTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAAAACCGCAATTGATGCGGCAGAGAAGCTCCt                                  >  2:5059681/1‑76 (MQ=255)

                                        tACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGa                                >  1:1749/1‑76 (MQ=255)

                                         acacCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGac                               <  1:2058100/76‑1 (MQ=255)

                                         acacCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGac                               <  2:2123291/76‑1 (MQ=255)

                                         acacCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGac                               <  1:6130713/76‑1 (MQ=255)

                                         acacCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGac                               <  2:2616186/76‑1 (MQ=255)

                                         acacCAAAGCGGAAGGTCCGGCGAAAAGCCAGTCGATGCTGAAAACCGCAATTGATGCGGCAGAGGTGAACCTCac                               >  2:4859790/1‑76 (MQ=255)

                                          cacCAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGaca                              <  1:5959616/76‑1 (MQ=255)

                                            ccAAGGGGGAAGGCCCGGCGAAAGGCCAGTCGATGCTGAACCCCGCAATTGATGCGGCAGAGATGACCCTGACACt                            <  2:5104600/76‑1 (MQ=255)

                                            ccAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACt                            <  2:1703647/76‑1 (MQ=255)

                                            ccAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACt                            >  2:3649164/1‑76 (MQ=255)

                                            ccAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACt                            <  2:1846225/76‑1 (MQ=255)

                                            ccAAAGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACt                            <  2:1241248/76‑1 (MQ=255)

                                              aaaGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGGGAGGATCCTGACACAgg                          >  2:4657754/1‑76 (MQ=255)

                                              aaaGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTgg                          >  1:1590797/1‑76 (MQ=255)

                                              aaaGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTgg                          >  1:4373238/1‑76 (MQ=255)

                                               aaGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGc                         >  2:1299652/1‑76 (MQ=255)

                                                aGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCa                        <  1:2437018/76‑1 (MQ=255)

                                                aGCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTAATGCGGCAGAGATGATCCTGACACTGGCa                        <  2:5350532/76‑1 (MQ=255)

                                                 gCGGAAGGTCGGTCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCAc                       <  2:55782/76‑1 (MQ=255)

                                                 gCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCAc                       <  1:5229262/76‑1 (MQ=255)

                                                 gCGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCAc                       <  1:3528347/76‑1 (MQ=255)

                                                  cGGAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCaac                      >  1:2003746/1‑74 (MQ=255)

                                                   ggAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCg                     >  2:3173224/1‑76 (MQ=255)

                                                    gAAGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCgg                    >  2:3378296/1‑76 (MQ=255)

                                                      aGGTCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGaa                  <  1:5409422/76‑1 (MQ=255)

                                                       ggTCCGGAGAAAGCCCAGTCGATGCTGAACCCCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGaaa                 <  1:5599007/76‑1 (MQ=38)

                                                         tCCGGCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAAcc               <  1:1104676/76‑1 (MQ=255)

                                                           cGGCGAAAGCCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCaa             <  1:3710291/76‑1 (MQ=255)

                                                            ggCGAAAGGCCGGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAAc            <  2:2484327/76‑1 (MQ=255)

                                                             gCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACg           <  1:1766954/76‑1 (MQ=255)

                                                             gCGAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAAATGATCCTGACACTGGCACCGGAAACCAACg           <  2:6206811/76‑1 (MQ=255)

                                                               gAAAGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGGTGATCCTGACACTGGCACCGGAAACCAACggc         >  2:4379546/1‑75 (MQ=255)

                                                                aaaGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTc        >  1:1608136/1‑76 (MQ=255)

                                                                aaaGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTc        >  1:1398884/1‑76 (MQ=255)

                                                                aaaGGCCAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTc        >  1:129322/1‑76 (MQ=255)

                                                                     ccAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAggga   >  2:851401/1‑74 (MQ=255)

                                                                     ccAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTa   <  1:683509/76‑1 (MQ=255)

                                                                      cAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAg  >  1:909426/1‑76 (MQ=255)

                                                                      cAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAg  >  1:5609378/1‑76 (MQ=255)

                                                                      cAGTCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAg  >  2:596250/1‑76 (MQ=255)

GAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAG  >  REL606/1282770‑1282915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`