| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,322,724 | G→A | pseudogene (385/408 nt) | yciQ → | hypothetical protein; b1268_1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,322,724 | 0 | G | A | 98.9% | 743.1 / ‑1.4 | 187 | pseudogene (385/408 nt) | yciQ | hypothetical protein; b1268_1 |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (104/81); total (105/82) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.47e-01 | |||||||||||
Only 100 of 187 total aligned reads displayed.
TCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTA > REL606/1322651‑1322791 | tCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAAt > 1:2609271/1‑76 (MQ=255)tCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAAt > 1:2472117/1‑76 (MQ=255) tGACTGGCATATCGGGTTAATGATTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAAtgct > 1:1123339/1‑76 (MQ=255) tGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAAtgct > 1:5488576/1‑76 (MQ=255) tGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAAtgct > 1:4428192/1‑76 (MQ=255) cTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGcc > 2:4820533/1‑76 (MQ=255) tGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCa < 1:3791120/76‑1 (MQ=255) tGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCa > 2:5223511/1‑76 (MQ=255) ggCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCac > 2:1801053/1‑75 (MQ=255) ggCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCaa > 1:4269088/1‑76 (MQ=255) ggCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCaa > 2:3427299/1‑76 (MQ=255) ggCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCaa < 1:3450552/76‑1 (MQ=255) gCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAAc < 1:3261823/76‑1 (MQ=255) cagatCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACt < 1:1002330/73‑1 (MQ=255) cATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACt < 1:4692685/76‑1 (MQ=255) cATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGGAATGCTGCCAACt > 2:2030918/1‑76 (MQ=255) cATATCGGGTTAATGAGTATTGTCGTGGGCCATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACt > 1:3293979/1‑76 (MQ=255) atatCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACtt > 2:4176852/1‑76 (MQ=255) atatCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACtt > 2:2209227/1‑76 (MQ=255) tatCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTa < 2:3836436/76‑1 (MQ=255) atCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTAc > 2:294341/1‑76 (MQ=255) atCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTAc > 1:5931713/1‑76 (MQ=255) atCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTAc < 1:6198718/76‑1 (MQ=255) tCGGGTTAGTGAGTATTGGCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACt < 1:3325878/76‑1 (MQ=39) cGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTg < 1:5772586/76‑1 (MQ=255) cGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTg < 1:6124886/76‑1 (MQ=255) cGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTg > 1:4179286/1‑76 (MQ=255) ggTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGAt > 1:2424636/1‑76 (MQ=255) ggTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGAt > 2:3208498/1‑76 (MQ=255) ggTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGAt > 2:38673/1‑76 (MQ=255) gTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGAtt > 2:4658640/1‑76 (MQ=255) gTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGAtt > 2:4951856/1‑76 (MQ=255) gTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGAtt > 2:1139559/1‑76 (MQ=255) ttAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGAttt > 1:1730864/1‑76 (MQ=255) tAATGAGTTTTGTCGTGTGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTa < 2:4686327/76‑1 (MQ=38) tAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTa < 1:6259848/76‑1 (MQ=255) tAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTa < 1:2180719/76‑1 (MQ=255) aaTGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAg < 2:819508/76‑1 (MQ=255) aTGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAgt < 1:1095528/76‑1 (MQ=255) aTGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAgt > 1:4668574/1‑76 (MQ=255) tGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAgtg < 2:1042058/76‑1 (MQ=255) tGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAgtg > 2:4484551/1‑76 (MQ=255) gAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAgtgt < 2:2473606/76‑1 (MQ=255) gAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAgtgt > 1:1626120/1‑76 (MQ=255) gTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTat < 2:2327502/76‑1 (MQ=255) gTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTat < 1:5101301/76‑1 (MQ=255) tATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTatg > 1:5599347/1‑76 (MQ=255) tATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTatg < 2:5661278/76‑1 (MQ=255) tATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTatg > 2:5075822/1‑76 (MQ=255) ttGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTatgat < 1:4086397/76‑1 (MQ=255) ttGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTatgat > 2:1236331/1‑76 (MQ=255) tGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTatgatg > 2:532587/1‑76 (MQ=255) tGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTatgatg > 2:5124307/1‑76 (MQ=255) gTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATgg > 1:3175662/1‑76 (MQ=255) gTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATgg > 1:438902/1‑76 (MQ=255) tCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGgt < 1:3607415/76‑1 (MQ=255) gggCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGttt < 2:3989758/76‑1 (MQ=255) gggCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGttt < 2:888469/76‑1 (MQ=255) gggCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGttt > 1:2025449/1‑76 (MQ=255) gggCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGttt > 1:6025017/1‑76 (MQ=255) gggCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGttt < 1:4630595/76‑1 (MQ=255) ggCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGtttt < 1:5883198/76‑1 (MQ=255) gCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGttttt < 1:3480784/76‑1 (MQ=255) gCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGttttt > 1:3994332/1‑76 (MQ=255) cGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTg < 2:1645859/76‑1 (MQ=255) cGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTg > 1:1563571/1‑76 (MQ=255) cGATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTg < 2:210337/76‑1 (MQ=255) gATAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGa < 1:4891248/76‑1 (MQ=255) aTAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAg > 2:171523/1‑76 (MQ=255) tAAACAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAgg > 2:3881975/1‑76 (MQ=255) aaaCAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGt > 1:3765402/1‑76 (MQ=255) aaaCAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGt > 1:1268672/1‑76 (MQ=255) aaaCAACGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGt > 1:1431741/1‑76 (MQ=255) aacaacGTTTCTTGCCTCAAGGCGACGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTg > 2:612135/1‑76 (MQ=255) aacaacGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTg < 2:2841830/76‑1 (MQ=255) aacaacGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTg > 2:3119487/1‑76 (MQ=255) caacGTTTCTTGCCTCAAGGCGACGGTACTGCTGCCAACTTACTGATTTAGTGTATGATGGTGCTTTTGAGGCGCt > 2:264436/1‑76 (MQ=18) acGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTcc > 1:9558/1‑76 (MQ=255) acGTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTcc < 2:4747216/76‑1 (MQ=255) gTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAg < 2:746685/76‑1 (MQ=255) gTTTCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAg > 2:4525472/1‑76 (MQ=255) tttCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGGGTTTTTGAGGTGCTCCAGt > 2:255433/1‑76 (MQ=255) ttCTTGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTg < 1:102231/76‑1 (MQ=255) ttGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCt > 1:2314852/1‑76 (MQ=255) tGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCtt > 1:4919631/1‑76 (MQ=255) tGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCtt > 1:1937443/1‑76 (MQ=255) tGCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCtt < 1:461542/76‑1 (MQ=255) gCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGGGGTGCTCCAGTGGCttt > 1:140857/1‑75 (MQ=255) gCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCt < 2:2678387/74‑1 (MQ=255) gCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCt > 1:2678387/1‑74 (MQ=255) gCCTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTc < 2:4185970/76‑1 (MQ=255) ccTCAAGGTGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCt < 1:2920466/76‑1 (MQ=255) cTCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTg < 1:5962812/76‑1 (MQ=255) tCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGt > 1:1532767/1‑76 (MQ=255) tCAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGt > 2:5932842/1‑76 (MQ=255) cAAGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGtt > 2:3891597/1‑76 (MQ=255) aaGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGttt < 2:6234577/76‑1 (MQ=255) aaGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGttt < 2:2407882/76‑1 (MQ=255) aaGGCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGttt < 2:4572450/76‑1 (MQ=255) gCGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTa < 1:3208498/76‑1 (MQ=17) | TCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTA > REL606/1322651‑1322791 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |