BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,360,063	A→G	P192P (CCA→CCG)	ycjL →	gamma‑Glu‑GABA hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,360,063	0	A	G	98.3%	711.6 / ‑0.7	175	P192P (CCA→CCG)	ycjL	gamma‑Glu‑GABA hydrolase
Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (101/71); total (102/73)
Fisher's exact test for biased strand distribution p-value = 5.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 179 total aligned reads displayed.

CTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGTG  >  REL606/1359990‑1360135

cTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCcgcg                                                                        >  2:5072181/1‑76 (MQ=255)

 tGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCgg                                                                       <  1:6283795/76‑1 (MQ=255)

 tGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCgg                                                                       >  1:6076635/1‑76 (MQ=255)

  gCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGt                                                                      >  1:2294703/1‑76 (MQ=255)

   cGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGtt                                                                     >  2:3521851/1‑76 (MQ=255)

   cGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGtt                                                                     >  1:1968255/1‑76 (MQ=255)

    gttgttACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTg                                                                    >  2:3914132/1‑76 (MQ=255)

    gttgttACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTg                                                                    >  2:6129446/1‑76 (MQ=255)

    gttgttACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTg                                                                    >  1:5827830/1‑76 (MQ=255)

     ttgttACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGc                                                                   >  2:3881296/1‑76 (MQ=255)

     ttgttACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGc                                                                   >  2:1224460/1‑76 (MQ=255)

      tgttACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCg                                                                  >  1:5073633/1‑76 (MQ=255)

      tgttACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCg                                                                  >  2:6107666/1‑76 (MQ=255)

       gttACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCgt                                                                 >  1:5168306/1‑76 (MQ=255)

        ttACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCgtg                                                                >  1:2558254/1‑76 (MQ=255)

        ttACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGAGCGAAGGTCGTTAGCACGCGGTTGCgtg                                                                >  2:2815794/1‑76 (MQ=255)

         tACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCgtgt                                                               >  2:2332411/1‑76 (MQ=255)

         tACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCgtgt                                                               >  1:6228885/1‑76 (MQ=255)

         tACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCgtgt                                                               >  1:1227561/1‑76 (MQ=255)

          aCCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGtt                                                              <  1:2431768/76‑1 (MQ=255)

          aCCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGtt                                                              <  1:5253241/76‑1 (MQ=255)

           ccTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTg                                                             >  2:1927074/1‑76 (MQ=255)

            cTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGa                                                            >  1:4640205/1‑76 (MQ=255)

             tGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGaa                                                           >  2:3770948/1‑76 (MQ=255)

                aTGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAgct                                                        >  1:5780728/1‑76 (MQ=255)

                aTGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAgct                                                        >  2:2918108/1‑76 (MQ=255)

                 tGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAgctc                                                       <  1:3323487/76‑1 (MQ=255)

                 tGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAgctc                                                       >  2:3195647/1‑76 (MQ=255)

                 tGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAgctc                                                       <  1:4598163/76‑1 (MQ=255)

                  gTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAgctcg                                                      <  2:2947236/76‑1 (MQ=255)

                   tAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGCAGGTAGTTAGCCCGCGGTTGCGTGTTGCAgatcgt                                                     >  2:1567752/1‑75 (MQ=255)

                   tAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAgctcgc                                                     >  2:1567753/1‑76 (MQ=255)

                   tAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAgctcgc                                                     >  1:1950000/1‑76 (MQ=255)

                    aGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAgctcgct                                                    <  2:2214685/76‑1 (MQ=255)

                    aGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAgctcgct                                                    <  1:3760288/76‑1 (MQ=255)

                    aGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAgctcgct                                                    >  2:4981795/1‑76 (MQ=255)

                      cAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGctcgctcg                                                  <  2:768860/76‑1 (MQ=255)

                      cAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGctcgctcg                                                  <  2:5790149/76‑1 (MQ=255)

                      cAACTGTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGctcgctcg                                                  <  2:340251/76‑1 (MQ=255)

                         cTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCg                                               <  1:2748894/76‑1 (MQ=255)

                         cTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCg                                               <  1:1085719/76‑1 (MQ=255)

                          ttttGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCgg                                              >  2:5759243/1‑76 (MQ=255)

                           tttGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGa                                             >  2:6231249/1‑76 (MQ=255)

                           tttGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGa                                             >  1:5605961/1‑76 (MQ=255)

                           tttGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGa                                             >  1:1414732/1‑76 (MQ=255)

                            ttGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGAt                                            <  2:4194979/76‑1 (MQ=255)

                            ttGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGAt                                            <  2:2025165/76‑1 (MQ=255)

                             tGGGTAACCTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATg                                           <  2:1699230/76‑1 (MQ=255)

                               ggTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGc                                         <  1:2589885/76‑1 (MQ=255)

                                 tAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCtt                                       <  2:3722438/76‑1 (MQ=255)

                                 tAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCtt                                       <  2:1376735/76‑1 (MQ=255)

                                  aaaCTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTa                                      >  1:1399401/1‑76 (MQ=255)

                                     ctctctACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGtt                                   <  2:4388668/76‑1 (MQ=255)

                                     ctctctACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGtt                                   <  1:5858140/76‑1 (MQ=255)

                                     ctctctACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGtt                                   <  2:2590572/76‑1 (MQ=255)

                                       ctctACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGGTTAGTTGa                                 >  2:5993669/1‑76 (MQ=255)

                                       ctctACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGa                                 >  1:939370/1‑76 (MQ=255)

                                        tctACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAg                                <  1:3815228/76‑1 (MQ=255)

                                         ctACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAgg                               <  2:3317741/76‑1 (MQ=255)

                                          tACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGc                              >  2:796319/1‑76 (MQ=255)

                                          tACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGc                              >  1:2120800/1‑76 (MQ=255)

                                          tACATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGc                              >  1:6177110/1‑76 (MQ=255)

                                           aCATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCg                             >  1:1824417/1‑76 (MQ=255)

                                           aCATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCg                             >  2:4761316/1‑76 (MQ=255)

                                           aCATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCg                             <  2:4223546/76‑1 (MQ=255)

                                            cATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGTGTGTTGAAGCTCGCTCGCCGGCTGGCTTAGTTGAGGCgg                            >  2:4736636/1‑76 (MQ=255)

                                            cATGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCgg                            >  1:1192166/1‑76 (MQ=255)

                                             aTGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCggg                           >  1:2779726/1‑75 (MQ=255)

                                             aTGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGt                           >  1:2357792/1‑76 (MQ=255)

                                             aTGGACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGt                           >  1:2760079/1‑76 (MQ=255)

                                               ggACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTa                         <  2:4065034/76‑1 (MQ=255)

                                               ggACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTa                         <  2:5844394/76‑1 (MQ=255)

                                               ggACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTa                         <  1:6181466/76‑1 (MQ=255)

                                               ggACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTa                         >  2:3069823/1‑76 (MQ=255)

                                                gACAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAg                        <  2:1141132/76‑1 (MQ=255)

                                                 aCAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGCGGCGGGTAGc                       >  2:5520654/1‑76 (MQ=255)

                                                 aCAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGttggg                       >  1:1748040/1‑73 (MQ=255)

                                                 aCAAGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGGTAgg                       >  2:4325422/1‑75 (MQ=255)

                                                   aaGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGt                     <  1:3200404/76‑1 (MQ=255)

                                                    aGGGGCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGGTAGCggc                    >  1:2438771/1‑74 (MQ=255)

                                                       ggCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGtcatc                 <  2:3261462/76‑1 (MQ=255)

                                                        gCGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGtcatca                <  1:601411/76‑1 (MQ=255)

                                                         cGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCatcaa               <  1:780728/76‑1 (MQ=255)

                                                         cGAAGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGCCatcaa               >  1:2661665/1‑76 (MQ=255)

                                                            aGGTCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGGGTCatccatca            >  1:2828880/1‑76 (MQ=255)

                                                               tCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGGCATCAATCATcc         >  1:3914005/1‑76 (MQ=255)

                                                               tCGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGGTGAGGCGGGTAGGGGCATCCAACATcc         >  2:332927/1‑76 (MQ=255)

                                                                cGTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGGGTCATCAATCATCCt        >  2:4947661/1‑76 (MQ=255)

                                                                 gTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCtt       <  1:2072351/76‑1 (MQ=255)

                                                                 gTTAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCtt       <  2:1824417/76‑1 (MQ=255)

                                                                  ttAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCttt      <  2:378112/76‑1 (MQ=255)

                                                                  ttAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCttt      <  1:1540415/76‑1 (MQ=255)

                                                                  tgaGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCttt      <  2:245166/74‑1 (MQ=255)

                                                                   tAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTTGAGCTTTTAATCATTCtttt     >  1:4116469/1‑76 (MQ=255)

                                                                   tAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCCTCAATTATCCtttt     >  2:2990645/1‑76 (MQ=255)

                                                                   tAGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCtttt     <  2:3279/76‑1 (MQ=255)

                                                                    aGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCttttt    >  1:3591608/1‑75 (MQ=255)

                                                                    aGCCCGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGGTAGGGTCATCAATCATCCCTTtg    >  2:2066913/1‑76 (MQ=255)

                                                                      cccGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCCTCCATCATCCTTTtgtg  >  1:2975366/1‑76 (MQ=255)

                                                                      cccGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTtgtg  >  1:3016729/1‑76 (MQ=255)

CTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGTG  >  REL606/1359990‑1360135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`