| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,440,659 | T→C | intergenic (+35/+24) | feaB → / ← tynA | phenylacetaldehyde dehydrogenase/tyramine oxidase, copper‑requiring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,440,659 | 0 | T | C | 99.5% | 907.7 / ‑5.5 | 219 | intergenic (+35/+24) | feaB/tynA | phenylacetaldehyde dehydrogenase/tyramine oxidase, copper‑requiring |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (119/99); total (119/100) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 230 total aligned reads displayed.
GGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGTGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAG > REL606/1440586‑1440731 | ggTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGgcgc > 2:741491/1‑76 (MQ=255) gTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGcc < 2:2671475/76‑1 (MQ=255) ttGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCg < 2:253332/76‑1 (MQ=255) tGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGt > 1:4573983/1‑76 (MQ=255) ggTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGtt > 2:2810018/1‑76 (MQ=255) tgtgAAACTAAGTCGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGtttt > 2:3835470/1‑76 (MQ=255) gAAACTAAGTCGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGttttttt > 2:1467039/1‑76 (MQ=255) gAAACTAAGTCGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGttttttt > 2:2399738/1‑76 (MQ=255) gAAACTAAGTCGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGttttttt < 2:4934828/76‑1 (MQ=255) cTAAGTCGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAGAAACGGCGCCTGGCGCCGTTTTTTTAGTc < 2:2382466/76‑1 (MQ=255) cTAAGTCGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAACAAAGGCGACTGGAGCCGTTTTGTTATTc > 2:2473307/1‑76 (MQ=255) cTAAGTCGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTc > 1:5537320/1‑76 (MQ=255) cTAAGTCGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTc > 2:52188/1‑76 (MQ=255) tAAGTCGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCt < 2:822780/76‑1 (MQ=255) aGTCGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGa > 1:3014711/1‑76 (MQ=255) gTCGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGaa > 1:6050818/1‑76 (MQ=255) cGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGaaag > 2:6170204/1‑75 (MQ=255) cGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAAc < 2:5277190/76‑1 (MQ=255) cGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAAc < 2:4274234/76‑1 (MQ=255) cGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAAc < 2:6270259/76‑1 (MQ=255) cGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAAc > 1:3525351/1‑76 (MQ=255) ggTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAAca > 2:3678846/1‑76 (MQ=255) ggTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAAca < 2:2912917/76‑1 (MQ=255) gtgtgtgtACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAAcaa > 1:3930331/1‑76 (MQ=255) gtgtgtgtACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAAcaa > 1:5699651/1‑76 (MQ=255) gtgtgtgtACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAAcaa < 1:4396610/76‑1 (MQ=255) gtgtgtgtACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAAcaa < 2:5689895/76‑1 (MQ=255) tgtttgtACGGGATTAATCCTGTTCGATCATTAGTAAAAAACGGCGCCTTGCGCCGTTTTTTTAGTCCGAAAaaat < 2:3100512/76‑1 (MQ=255) tgtgtgtACGGTATTAATCTTGTTCGCTCATAAGTAAAAACCGGCGCCTGGCCCCGTTTTTTTAGTCTGAAAcaat < 1:1774385/76‑1 (MQ=255) gtgtgtACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAAcaatc < 2:1320997/76‑1 (MQ=255) gtgtgtACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAAcaatc < 2:2146551/76‑1 (MQ=255) gtgtgtACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAAcaatc > 2:2740825/1‑76 (MQ=255) gtgtgtACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAAcaatc < 1:2404619/76‑1 (MQ=255) gtgtgtACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAAcaata < 2:2479080/76‑2 (MQ=255) tgtgtACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAAcaatca > 2:653185/1‑76 (MQ=255) tgtgtACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAAcaatca < 2:5483626/76‑1 (MQ=255) tgtgtACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAAcaatca > 2:38236/1‑76 (MQ=255) tgtACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAAcaatcaat > 1:102755/1‑76 (MQ=255) gtACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAAtt > 2:5890537/1‑76 (MQ=255) gtACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAAtt < 1:3066996/76‑1 (MQ=255) gtACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAAtt > 2:134778/1‑76 (MQ=255) gtACGGTATTAATCTTGTTCGCCCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAAtt > 2:3461007/1‑76 (MQ=39) tACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTa > 1:3692953/1‑76 (MQ=255) tACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTa > 2:1481384/1‑76 (MQ=255) tACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTa > 2:1560723/1‑76 (MQ=255) aCGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTAt < 2:3495536/76‑1 (MQ=255) aCGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTAt < 2:4380599/76‑1 (MQ=255) cGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTAtc > 2:1914638/1‑76 (MQ=255) cGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTAtc < 1:2129638/76‑1 (MQ=255) gTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTAtctt > 2:4705165/1‑76 (MQ=255) gTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTAtctt > 1:4834761/1‑76 (MQ=255) tATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTAtcttt < 1:1717357/76‑1 (MQ=255) tAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTAtctttctt > 1:5765708/1‑76 (MQ=255) tAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTAtctttctt < 1:52663/76‑1 (MQ=255) tAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTAtctttctt < 2:5160250/76‑1 (MQ=255) tAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTAtctttctt > 1:2704484/1‑76 (MQ=255) aaTCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTttcttc > 1:1422208/1‑76 (MQ=255) aaTCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTttcttc > 2:899861/1‑76 (MQ=255) aaTCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTttcttc > 1:998739/1‑76 (MQ=255) aaTCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTttcttc > 2:1745541/1‑76 (MQ=255) atattGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGATACCGTCAATTATCTTTCTTCa > 2:5586309/4‑76 (MQ=38) aTCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCa < 2:5932326/76‑1 (MQ=255) aTCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCa < 1:1978975/76‑1 (MQ=255) aTCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCa > 1:2495302/1‑76 (MQ=255) aTCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCa > 2:2975359/1‑76 (MQ=255) tCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAg > 1:520960/1‑76 (MQ=255) cTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAgc > 1:4246677/1‑76 (MQ=255) ttGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAgcg > 1:4829156/1‑76 (MQ=255) tGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAgcgc > 2:2568530/1‑76 (MQ=255) tGTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAgcgc > 1:5645004/1‑76 (MQ=255) gTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGcc > 1:5143835/1‑76 (MQ=255) gTTCGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGcc > 2:5748998/1‑76 (MQ=255) cGCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCt < 1:2609609/76‑1 (MQ=255) gcccATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTa < 1:3461007/73‑1 (MQ=255) gCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTa > 2:4434034/1‑76 (MQ=255) gCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTa < 1:134778/76‑1 (MQ=255) gCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTa < 1:549674/76‑1 (MQ=255) gCTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTa < 2:1140373/76‑1 (MQ=255) cTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAg < 1:3239762/76‑1 (MQ=255) cTCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAg > 2:5848406/1‑76 (MQ=255) tCATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAGc > 2:3332302/1‑76 (MQ=255) cATAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAGCg > 2:2281872/1‑76 (MQ=255) aTAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAGCGt > 2:4471434/1‑76 (MQ=255) aTAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAGCGt > 2:1597447/1‑76 (MQ=255) aTAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAGCGt > 2:2896894/1‑76 (MQ=255) aTAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAGCGt > 2:3623429/1‑76 (MQ=255) tAAGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAGCGtt > 2:468279/1‑76 (MQ=255) aaGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAGCGTTg > 1:2811097/1‑76 (MQ=255) aaGTAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAGCGTTg > 1:188342/1‑76 (MQ=255) tAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAGCGTTGGCg < 2:3282022/76‑1 (MQ=255) tAAAAAACGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAGCGTTGGCg < 1:5230339/76‑1 (MQ=255) aaaaaaCGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAGCGTTGGCGt > 2:4843919/1‑76 (MQ=255) aaCGGCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCg > 2:4916340/1‑76 (MQ=255) cGGCGCCTTGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTc > 2:3091703/1‑76 (MQ=255) ggCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCa < 2:5976441/76‑1 (MQ=255) ggCGCCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCa < 1:4185489/76‑1 (MQ=255) gcgcCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCaa < 1:3757662/76‑1 (MQ=255) gcCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAaag < 1:6237633/76‑1 (MQ=255) gcCTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAaag < 1:5077596/76‑1 (MQ=255) cTGGCGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAaagaa < 2:2196640/76‑1 (MQ=255) | GGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTTGTTCGCTCATAAGTAAAAAACGGCGCCTGGTGCCGTTTTTTTAGTCTGAAACAATCAATTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAG > REL606/1440586‑1440731 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |