| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,076,609 | G→A | T46T (ACG→ACA) | yegE → | predicted diguanylate cyclase, GGDEF domain signalling protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,076,609 | 0 | G | A | 99.4% | 649.0 / ‑4.0 | 162 | T46T (ACG→ACA) | yegE | predicted diguanylate cyclase, GGDEF domain signalling protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (85/76); total (85/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.79e-01 | |||||||||||
Only 100 of 168 total aligned reads displayed.
TAGGTTTAGTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTG > REL606/2076536‑2076681 | taggtttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATc > 2:2784400/1‑76 (MQ=255)taggtttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATc < 2:1553961/76‑1 (MQ=255)taggtttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATc > 2:5321742/1‑76 (MQ=255) aggtttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATcc < 1:3210090/76‑1 (MQ=255) ggtttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCca > 1:5158677/1‑76 (MQ=255) gtttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCcat < 2:3220596/76‑1 (MQ=255) tttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGAcatccatc > 2:2945990/1‑76 (MQ=255) tttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGAcatccatc > 1:1481337/1‑76 (MQ=255) ttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCcatca > 1:1613180/1‑76 (MQ=255) tagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCcatcat < 2:2459043/76‑1 (MQ=255) agTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCTGACATCCATCatg > 2:3986024/1‑76 (MQ=255) agTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCatg > 2:5574831/1‑76 (MQ=255) gTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCatga < 1:4300783/76‑1 (MQ=255) tctcGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCatgat < 1:6105780/76‑1 (MQ=255) ctcGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCatgatg > 2:6055522/1‑76 (MQ=255) tcGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGAtgg > 1:5675713/1‑76 (MQ=255) tcGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGAtgg < 2:101968/76‑1 (MQ=255) tcGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCACGACATCCATCATGAtgg > 2:50426/1‑76 (MQ=255) cGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGAtggt > 1:3386565/1‑76 (MQ=255) cGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGAtggt > 2:4847141/1‑76 (MQ=255) gTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGAtggtg < 2:3856759/76‑1 (MQ=255) gTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGAtggtg < 1:1799985/76‑1 (MQ=255) gTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGAtggtg < 2:4417025/76‑1 (MQ=255) tttATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGAtggtgg > 2:5984407/1‑76 (MQ=255) tttATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGAtggtgg > 1:2546975/1‑76 (MQ=255) tttATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGAtggtgg < 2:6300756/76‑1 (MQ=255) ttATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGc < 1:1768329/76‑1 (MQ=255) tATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCg < 1:2237466/76‑1 (MQ=255) tCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGtt > 2:5668369/1‑76 (MQ=255) cTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGttt > 2:5364868/1‑76 (MQ=255) cTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGttt > 2:2105038/1‑76 (MQ=255) ttACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTa < 2:243550/76‑1 (MQ=255) tACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTAt > 1:1009018/1‑76 (MQ=255) tACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTAt > 2:2165306/1‑76 (MQ=255) tACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTAt > 2:2529383/1‑76 (MQ=255) tACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTAt > 1:3073366/1‑76 (MQ=255) aCCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATc > 1:6007376/1‑76 (MQ=255) aCCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATc > 2:135247/1‑76 (MQ=255) aCCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATc > 1:5309804/1‑76 (MQ=255) cccTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCg < 1:2147977/76‑1 (MQ=255) ttttCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCa < 2:3097622/76‑1 (MQ=255) ttCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATg < 2:1837941/76‑1 (MQ=255) ttCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATg > 2:585096/1‑76 (MQ=255) ttCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATg > 1:1422924/1‑76 (MQ=255) ttCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATg < 2:4934617/76‑1 (MQ=255) ttCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATg > 1:5538726/1‑76 (MQ=255) tctcGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGCTGGCGTTTTATCGCCATGc > 1:3576116/1‑76 (MQ=255) tcGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCg > 1:4481275/1‑76 (MQ=255) tcGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCg > 1:5730503/1‑76 (MQ=255) cGCTTGGGCTATCGCAGTTTGCCCAACTGTGGTGCCCGACATAGATCTTGATGATGGCGCTTTATCGCCATGTCgg > 2:2514107/1‑76 (MQ=255) cGCTTGAGCTTTCTGAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCgg < 2:2972062/76‑1 (MQ=255) cTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGgc > 1:5121732/1‑76 (MQ=255) ttGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGgcg > 2:507691/1‑76 (MQ=255) ttGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGgcg > 1:4872291/1‑76 (MQ=255) ttGAGCTTTCGCAGTTTGCCCCACTGTGATTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGgcg < 2:2700007/76‑1 (MQ=255) cTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCAtgt > 2:81337/1‑76 (MQ=255) cTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCAtgt < 1:1925470/76‑1 (MQ=255) tttCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCAtgtg > 2:3650613/1‑76 (MQ=255) tttCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCAtgtg < 2:4977642/76‑1 (MQ=255) tttCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCAtgtg > 1:3950809/1‑76 (MQ=255) tttCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCAtgtg > 2:297747/1‑76 (MQ=255) ttCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTgg < 2:1895201/76‑1 (MQ=255) ttCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTgg < 1:1537934/76‑1 (MQ=255) ttCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTgg < 1:599992/76‑1 (MQ=255) ttCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTgg < 2:5791846/76‑1 (MQ=255) cGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGcc < 1:5848798/76‑1 (MQ=255) cGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGcc < 2:2233703/76‑1 (MQ=255) gCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCg > 2:3415115/1‑76 (MQ=255) cAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCgg > 2:2116019/1‑76 (MQ=255) aGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCggg < 1:4090420/76‑1 (MQ=255) aGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCggg > 2:6213071/1‑76 (MQ=255) tttGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGaa > 1:2415938/1‑76 (MQ=255) ttGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAAt > 1:6307523/1‑76 (MQ=255) ccccACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTgc < 1:6047080/76‑1 (MQ=255) cccACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTgcg < 1:2732061/76‑1 (MQ=255) cccACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTgcg > 2:1469820/1‑76 (MQ=255) cACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCt < 2:2231890/76‑1 (MQ=255) tcTGTGGTTCCCGGCATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTg < 2:4934181/75‑1 (MQ=255) aCTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTg > 1:803897/1‑76 (MQ=255) aCTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTg < 2:5740493/76‑1 (MQ=255) cTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGa > 2:5513721/1‑76 (MQ=255) tgGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCt > 1:1075043/1‑76 (MQ=255) tgGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCt < 1:196880/76‑1 (MQ=255) tgGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCt < 2:1387081/76‑1 (MQ=255) ggTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTg < 2:1935310/76‑1 (MQ=255) ggTTCCCGACATCCATCATGATGGTGGCGTTTTAACGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTg > 2:5214423/1‑76 (MQ=255) gTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGt > 1:4568262/1‑76 (MQ=255) ttCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGtt < 2:27156/76‑1 (MQ=255) ttCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGtt < 1:5275436/76‑1 (MQ=255) tCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTc > 2:4557884/1‑76 (MQ=255) tCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTc > 1:245921/1‑76 (MQ=255) cccGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCg > 1:2503762/1‑76 (MQ=255) cccGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCg < 2:6064036/76‑1 (MQ=255) cccGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCg > 1:1864299/1‑76 (MQ=255) ccGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGc > 2:3691778/1‑76 (MQ=255) ccGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGc < 2:6309093/76‑1 (MQ=255) cGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCt < 1:611309/76‑1 (MQ=255) cGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCt < 2:840318/76‑1 (MQ=255) gACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTg < 1:737830/76‑1 (MQ=255) gACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTg < 1:298673/76‑1 (MQ=255) | TAGGTTTAGTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTG > REL606/2076536‑2076681 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |