| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,173,730 | G→A | R103H (CGT→CAT) | dld → | D‑lactate dehydrogenase, FAD‑binding, NADH independent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,173,730 | 0 | G | A | 99.4% | 622.6 / ‑3.9 | 157 | R103H (CGT→CAT) | dld | D‑lactate dehydrogenase, FAD‑binding, NADH independent |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (71/85); total (72/85) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.79e-01 | |||||||||||
Only 100 of 162 total aligned reads displayed.
ACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGCT > REL606/2173657‑2173802 | aCAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCAtc < 2:5676430/76‑1 (MQ=255)aCAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCAtc < 1:3420618/76‑1 (MQ=255) cAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCAtct < 1:6285026/76‑1 (MQ=255) cAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCAtct < 1:2684045/76‑1 (MQ=255) aGGCCTGACCGAAGGATCGACGCCAAACGGTCACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCAtctc > 2:2061792/1‑76 (MQ=255) aGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCAtctc < 1:1010596/76‑1 (MQ=255) ggCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCg > 1:576910/1‑76 (MQ=255) ggCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCg < 2:4210002/76‑1 (MQ=255) ccTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGAc < 1:4976945/76‑1 (MQ=255) ccTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGAc < 1:3851/76‑1 (MQ=255) ccTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGAc < 1:2660711/76‑1 (MQ=255) ccTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGAc > 2:645414/1‑76 (MQ=255) ccTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGAc > 2:6112898/1‑76 (MQ=255) cggACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACa < 1:4227214/74‑1 (MQ=255) cTGCCCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACa < 2:1968156/76‑1 (MQ=255) gACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAg < 2:5480110/76‑1 (MQ=255) gAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGc > 2:1753323/1‑76 (MQ=255) aaGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCCCCCTGCATCTCGACAAGCTGCa < 2:4456325/76‑1 (MQ=255) aaGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCa > 2:4426033/1‑76 (MQ=255) aaGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCa > 2:3460432/1‑76 (MQ=255) aGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCAc < 1:3782325/76‑1 (MQ=255) aTCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGtt > 2:3172158/1‑76 (MQ=255) aTCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGtt > 1:631865/1‑76 (MQ=255) tCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTc > 2:5934196/1‑76 (MQ=255) tCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTc > 2:68935/1‑76 (MQ=255) gACGCCAAACGGTAACGATTATGATCGCGGTGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCATGTTCtt > 1:4419205/1‑76 (MQ=255) cGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTgg < 1:508516/76‑1 (MQ=255) cGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCACCTCGACAAGCTGCACGTTCTTgg < 2:1265804/76‑1 (MQ=255) ccAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACaa > 1:5137827/1‑59 (MQ=255) ccAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACaa < 2:5137827/59‑1 (MQ=255) ccAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCa > 2:4171390/1‑76 (MQ=255) ccAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCa < 1:2747458/76‑1 (MQ=255) cAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCaa > 2:3881937/1‑76 (MQ=255) cAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCaa > 2:880964/1‑76 (MQ=255) cAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCaa < 1:2496863/76‑1 (MQ=255) aaaCGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAg < 2:2090393/76‑1 (MQ=255) aaaCGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAg > 1:4525349/1‑76 (MQ=255) aaaCGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAg < 1:451792/76‑1 (MQ=255) aaCGGTAACGATTATCATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAgg < 1:186885/76‑1 (MQ=255) cGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGc > 1:4874837/1‑76 (MQ=255) cGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGc < 1:4043108/76‑1 (MQ=255) cGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGc > 1:3264689/1‑76 (MQ=255) ggTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCg < 1:5308228/76‑1 (MQ=255) gTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGa > 2:5405403/1‑76 (MQ=255) tAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGaa > 2:4239998/1‑76 (MQ=255) tAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGaa < 2:4981514/76‑1 (MQ=255) tAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGaa > 2:1341646/1‑76 (MQ=255) aaCGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAAc < 1:4827010/76‑1 (MQ=255) aaCGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAAc < 1:2233621/76‑1 (MQ=255) aaCGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAAc < 1:2105691/76‑1 (MQ=255) aCGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACa < 2:3744888/76‑1 (MQ=255) cGATTATGAGCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAg < 1:4671205/76‑1 (MQ=255) aTTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAggg > 2:5767893/1‑75 (MQ=255) ttATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAGCAGGTg > 2:105198/1‑76 (MQ=255) ttATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTg < 2:346438/76‑1 (MQ=255) ttATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGAGAACAggag > 1:3698819/1‑74 (MQ=255) aTGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGCACAGGTGCt > 2:1984995/1‑76 (MQ=255) aTGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCt > 2:4910430/1‑76 (MQ=255) aTCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGc < 1:1692874/76‑1 (MQ=255) cgcgATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCt > 1:3358189/1‑76 (MQ=255) cgcgATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCt < 1:3551182/76‑1 (MQ=255) gcgATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTa < 1:1550481/76‑1 (MQ=255) gcgATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTa < 2:3986180/76‑1 (MQ=255) cgATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTAt < 1:564067/76‑1 (MQ=255) cgATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTAt < 1:3953654/76‑1 (MQ=255) cgATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAAAAGGTGCTGGCCTAt > 2:5871858/1‑76 (MQ=255) gATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATc > 1:995061/1‑76 (MQ=255) gATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATc < 1:1361838/76‑1 (MQ=255) aTGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATcc < 2:1158340/76‑1 (MQ=255) aTGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATcc < 2:1511597/76‑1 (MQ=255) aTGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATcc > 2:1396868/1‑76 (MQ=255) tGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCg < 2:4687981/76‑1 (MQ=255) gTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTTGCCTATcctg > 2:752381/1‑74 (MQ=255) cGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGc > 2:3310962/1‑76 (MQ=255) gTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGCACAGGTGCCGGCCTATCCGCGca > 1:2644572/1‑76 (MQ=255) gTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGca > 2:4581647/1‑76 (MQ=255) gTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGca > 1:5802098/1‑76 (MQ=255) gTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGca > 1:2921071/1‑76 (MQ=255) gTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGca > 2:112744/1‑76 (MQ=255) tATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGcacc > 1:5327827/1‑76 (MQ=255) tATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGcacc < 1:6190014/76‑1 (MQ=255) tATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGcacc > 1:1754612/1‑76 (MQ=255) tATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGcacc > 1:5524427/1‑76 (MQ=255) atcatcAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGcacca > 1:6096310/1‑76 (MQ=255) atcatcAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGcacca < 2:4138849/76‑1 (MQ=255) atcatcAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGcacca < 1:1283514/76‑1 (MQ=255) tcatcaGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGGGCTGGCCTATCCGGGcaccac > 1:187765/1‑76 (MQ=255) catcaGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACg > 1:3814871/1‑76 (MQ=255) atcaGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGc > 1:1155112/1‑76 (MQ=255) tcaGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGct < 2:287352/76‑1 (MQ=255) tcaGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGcc > 2:2539938/1‑75 (MQ=255) aGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGctct < 1:4215022/76‑1 (MQ=255) cACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTAt < 2:1147829/76‑1 (MQ=255) aCCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTAtt > 2:3785591/1‑76 (MQ=255) ccTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCg < 1:3968196/76‑1 (MQ=255) cTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGc < 2:1929746/76‑1 (MQ=255) cTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGc > 1:6082428/1‑76 (MQ=255) cTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGc < 1:6050982/76‑1 (MQ=255) tGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGCt < 2:467679/76‑1 (MQ=255) tGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGCGCCGGCCTATCCGGGCACCACCCTCTATTCGCt > 1:5560117/1‑76 (MQ=255) | ACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGCT > REL606/2173657‑2173802 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |