BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	603.2 / NA	160	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (55/105); total (55/105)

Only 100 of 184 total aligned reads displayed.

CCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193707‑2193851

 cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATcccc                                                                      <  2:9464/76‑1 (MQ=255)

 cGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGT‑CGGGAAACAGCCCCAATAACAAC‑AAAACGCCCAAcccc                                                                      >  1:6105266/1‑76 (MQ=255)

 cGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGT‑CGGGAAAAAACCCCAAAAACAAC‑AAAAAACCAAAcccc                                                                      >  2:2102588/1‑76 (MQ=255)

   aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCccac                                                                    <  1:1116144/76‑1 (MQ=255)

   aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCccac                                                                    <  1:3522747/76‑1 (MQ=255)

    tGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAACCGCCAATCCccacc                                                                   <  1:1139819/76‑1 (MQ=255)

    tGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGG‑CGGAAATCAGCCCCAATAACAGC‑AAAAAGCCAAcacccacc                                                                   >  1:5807080/1‑76 (MQ=255)

     gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCccacca                                                                  <  1:3682187/76‑1 (MQ=255)

     gCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGT‑CGGGAAACAGCCCCCATAAAAGC‑AAAACGCCAAACCccacca                                                                  >  1:2830757/1‑76 (MQ=255)

      cATCCGGCGTCCAGGCGCGGCTGGTCTTGCTGG‑CGGGAATCAGCCCCCATAAAAGC‑AAAACACCAAACCCCCCCAg                                                                 >  2:3427820/1‑76 (MQ=255)

      cATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTGCGGAAA‑CAGCCCCCACAAACGCAAAAACCCCAAccccccccg                                                                  >  1:3906753/1‑75 (MQ=255)

      cATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGG‑CGGAAAAAAGCCCCCATAAAAACAAAAACCCCAAccccccccg                                                                  >  2:5010630/1‑75 (MQ=255)

       aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGa                                                                <  2:2940830/76‑1 (MQ=255)

        tCCGGCGTCCGGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGAt                                                               <  2:4301654/76‑1 (MQ=255)

        tCCGGCGTCCAGGCGCGGCGTGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGAt                                                               <  1:3052389/76‑1 (MQ=255)

          cagCGTCCAGGCGCGGCTGGTCTGGCTGG‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATaa                                                             <  2:4290453/74‑1 (MQ=255)

          cGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATaa                                                             <  1:2109045/76‑1 (MQ=255)

          cGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATaa                                                             <  1:5167016/76‑1 (MQ=255)

          cGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATaa                                                             <  1:5732385/76‑1 (MQ=255)

          cGGCGTCCAGGCGCGGCTGGGCTGGCTGT‑CGGGAAAGAGCCCCCAAAACAAC‑AAAAAGCCCATCCCCCCCAGAtta                                                             >  2:4136156/1‑74 (MQ=255)

          cGGCGTCCAGGCGCGGCTGGGCTGGCTGT‑CGGAAAACAGCCCCAATAAACGC‑AAAACACCAACACCCCCCCGATaa                                                             >  1:4256737/1‑76 (MQ=255)

          cGGCGTCCAGGCGCGGCTGGGCTGGCTGG‑CGGGAAACAGCCCCAATAACAGG‑CAAACGCCAATCCCCCCCACATaa                                                             >  2:5671154/1‑76 (MQ=255)

          cGGCGTCCAGGCGCGGCTGGGCTGGCTGG‑CGGGAAACAGCCCCAAAAAAAGC‑AAAACGCCAAACCCCACCCGATaa                                                             >  1:3963457/1‑76 (MQ=255)

           ggCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATaaa                                                            <  1:6264593/76‑1 (MQ=255)

            gCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAg                                                           <  1:4766875/76‑1 (MQ=255)

             cGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGc                                                          <  1:2456785/76‑1 (MQ=255)

             cGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGc                                                          <  2:6086633/76‑1 (MQ=255)

             cGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGc                                                          <  1:4634340/76‑1 (MQ=255)

              gTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCa                                                         <  1:5409724/76‑1 (MQ=255)

                ccAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATg                                                       <  2:132279/76‑1 (MQ=255)

                ccAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATg                                                       <  1:2527136/76‑1 (MQ=255)

                 cAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGa                                                      <  2:1389429/76‑1 (MQ=255)

                  aGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGAc                                                     <  1:1394301/76‑1 (MQ=255)

                    gcgcGGCATGTCTGTCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGc                                                   <  2:5195908/76‑1 (MQ=255)

                     cgcgGCTGGGCTGGGTGT‑CGGGAATCAGCCCCAATAACACC‑AAAAAGCCAAATCCCACCAGCAAAAGCATGACGcc                                                  >  2:3866864/1‑76 (MQ=255)

                       cgGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                                <  2:4154705/76‑1 (MQ=255)

                         gCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                              <  2:4939473/76‑1 (MQ=255)

                         gCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                              <  1:323415/76‑1 (MQ=255)

                          cTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             <  1:4924520/76‑1 (MQ=255)

                          cTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             <  1:5291046/76‑1 (MQ=255)

                          cTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             <  2:3273912/76‑1 (MQ=255)

                           tGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            >  1:4512634/1‑76 (MQ=255)

                           tGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            >  2:634150/1‑76 (MQ=255)

                           tGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            <  1:4062613/76‑1 (MQ=255)

                           tGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            <  2:3967232/76‑1 (MQ=255)

                            ggTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           >  1:5195029/1‑76 (MQ=255)

                             gTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          <  1:1844043/76‑1 (MQ=255)

                             gTCTGACTTT‑CGGAAATCAGACCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          <  1:4444569/76‑1 (MQ=255)

                              tCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         <  1:3525638/76‑1 (MQ=255)

                              tCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         >  2:4611691/1‑76 (MQ=255)

                              tCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         <  2:5280337/76‑1 (MQ=255)

                               cTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        <  1:2931302/76‑1 (MQ=255)

                                tGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       >  2:743668/1‑76 (MQ=255)

                                tGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       <  2:3810253/76‑1 (MQ=255)

                                tGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       <  1:2939437/76‑1 (MQ=255)

                                 ggCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                      >  1:1427300/1‑76 (MQ=255)

                                   cTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                    <  2:784916/76‑1 (MQ=255)

                                   cTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                    >  2:4812011/1‑76 (MQ=255)

                                    tGT‑CGGAAATCAGCCCCAATAACAGG‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                   <  2:4879777/76‑1 (MQ=255)

                                    tGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACCGGCt                                   >  1:2223181/1‑76 (MQ=255)

                                    tGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                   >  2:1954808/1‑76 (MQ=255)

                                    tGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                   >  2:2992756/1‑76 (MQ=255)

                                    tGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                   <  2:5908665/76‑1 (MQ=255)

                                    tGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                   >  1:3959805/1‑76 (MQ=255)

                                      t‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 >  1:6170761/1‑76 (MQ=255)

                                        cGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                >  2:1812077/1‑76 (MQ=255)

                                        cGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                <  1:853284/76‑1 (MQ=255)

                                         ggAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  2:6019624/76‑1 (MQ=255)

                                          gAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  1:3084495/76‑1 (MQ=255)

                                          gAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  2:4005047/76‑1 (MQ=255)

                                           aaaTCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             <  2:2516399/76‑1 (MQ=255)

                                             aTCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           <  2:823470/76‑1 (MQ=255)

                                               cAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         >  2:2279669/1‑76 (MQ=255)

                                               cAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         >  2:1707507/1‑76 (MQ=255)

                                               cAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         <  2:6105266/76‑1 (MQ=255)

                                               cAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         <  1:6100622/76‑1 (MQ=255)

                                                aGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                        <  1:2819218/76‑1 (MQ=255)

                                                  ccccAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                      >  1:1675795/1‑76 (MQ=255)

                                                  ccccAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                      >  2:5095027/1‑76 (MQ=255)

                                                   cccAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg                     <  2:2666013/76‑1 (MQ=255)

                                                   cccAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg                     <  1:4008109/76‑1 (MQ=255)

                                                     cAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa                   <  2:3897483/76‑1 (MQ=255)

                                                      aaTAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                  <  2:5405940/76‑1 (MQ=255)

                                                      aaTAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                  >  2:5648982/1‑76 (MQ=255)

                                                       aTAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 <  1:3488544/76‑1 (MQ=255)

                                                        tAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                >  1:3444315/1‑76 (MQ=255)

                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         >  2:4432820/1‑76 (MQ=255)

                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  2:3919373/76‑1 (MQ=255)

                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  1:5337722/76‑1 (MQ=255)

                                                                 aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg        <  2:5684953/76‑1 (MQ=255)

                                                                  aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       >  2:1078699/1‑76 (MQ=255)

                                                                  aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       >  1:5637013/1‑76 (MQ=255)

                                                                   aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc      <  2:357415/76‑1 (MQ=255)

                                                                      ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCCTTTTACCGATGGCGCATCGCGCACCAgcc      <  2:1450541/73‑1 (MQ=255)

                                                                      ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCCTTTTACCGATGGCGCATCGCGCACCAgcc      >  1:1450541/1‑73 (MQ=255)

                                                                       cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  1:2188455/1‑76 (MQ=255)

                                                                       cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  2:2133226/1‑76 (MQ=255)

                                                                       cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  2:2143169/1‑76 (MQ=255)

                                                                       cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  2:2844215/1‑76 (MQ=255)

                                                                       cAACCCCCACCAGATAAAGCATGACGCCTTTATTAAACAGGCTTTTTACCGATGGCGCACCCCCCCCCAGCCGCca  <  2:6112146/76‑1 (MQ=255)

CCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193707‑2193851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`