| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,577,214 | T→C | intergenic (‑37/+21) | pepB ← / ← yfhJ | aminopeptidase B/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,577,214 | 0 | T | C | 99.1% | 852.8 / ‑2.6 | 214 | intergenic (‑37/+21) | pepB/yfhJ | aminopeptidase B/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (142/70); total (144/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01 | |||||||||||
Only 100 of 220 total aligned reads displayed.
GTTGGGTAGAGAGGGTAATTTTCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCTACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTCGAGGATTTTCT > REL606/2577141‑2577284 | gTTGGGTAGAGAGGGTAATTTTCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCAc < 2:4481990/76‑1 (MQ=255) tGGGTAGAGAGGGTAATTTTCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCAccc > 2:1169392/1‑76 (MQ=255) gggTAGAGAGGGTAATTTTCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCAcccc < 2:69475/76‑1 (MQ=255) gggTAGAGAGGGTAATTTTCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCAcccc < 2:1065329/76‑1 (MQ=255) gggTAGAGAGGGTAATTTTCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCAcccc > 2:5209315/1‑76 (MQ=255) ggTAGAGAGGGTAATTTTCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCa < 2:3241868/76‑1 (MQ=255) ggTAGAGAGGGTAATTTTCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCa > 2:2576454/1‑76 (MQ=255) tAGAGAGGGTAATTTTCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAgg > 2:3629583/1‑76 (MQ=255) tAGAGAGGGTAATTTTCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAgg < 2:330134/76‑1 (MQ=255) tAGAGAGGGTAATTTTCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAgg > 2:2825006/1‑76 (MQ=255) tAGAGAGCGTAATTTTCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCCCCCCAgg < 2:5235398/76‑1 (MQ=255) agagagGGTAATTTTCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGc > 1:2285254/1‑76 (MQ=255) agagagGGTAATTTTCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGc > 2:5722558/1‑76 (MQ=255) agagagGGTAATTTTCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGc > 1:4063008/1‑76 (MQ=255) gagagGGTAATTTTCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCa < 1:5527063/76‑1 (MQ=255) gagagGGTAATTTTCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCa < 2:2135407/76‑1 (MQ=255) agagGGTAATTTTCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAg > 2:2716282/1‑76 (MQ=255) gggTAATTTTCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGccc < 2:3700430/76‑1 (MQ=255) aaTTTTCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGttt > 2:5773021/1‑76 (MQ=255) tttCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTatt < 2:5492048/76‑1 (MQ=255) tttCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTatt < 2:3941575/76‑1 (MQ=255) tttCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTatt > 1:5498917/1‑76 (MQ=255) tttCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTatt > 1:5235694/1‑76 (MQ=255) ttCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTattc < 2:2462251/76‑1 (MQ=255) tCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTattca > 2:3872704/1‑76 (MQ=255) cATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTattcat > 2:3051618/1‑76 (MQ=255) cATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTattcat > 1:4698569/1‑76 (MQ=255) tCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTattcattc < 1:1335299/76‑1 (MQ=255) cGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCg > 2:4825431/1‑76 (MQ=255) cGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCg > 1:3764076/1‑76 (MQ=255) cGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCg > 2:2152707/1‑76 (MQ=255) ttCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGcc < 2:800781/76‑1 (MQ=255) tCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCt > 2:5025233/1‑76 (MQ=255) tCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCt > 1:223852/1‑76 (MQ=255) tCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCt > 1:648664/1‑76 (MQ=255) cTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTc < 2:6031548/76‑1 (MQ=255) tGTCATTTTAGTTATCCTTATTCGCTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCg > 2:1942688/1‑76 (MQ=255) gTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGt < 2:5911582/76‑1 (MQ=255) tCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTc > 1:167007/1‑76 (MQ=255) tCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTc > 1:4219195/1‑76 (MQ=255) tCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTc < 2:5315000/76‑1 (MQ=255) cATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTcc > 1:224740/1‑76 (MQ=255) cATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTcc > 1:5767253/1‑76 (MQ=255) aTTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTcca > 2:4021044/1‑76 (MQ=255) aTTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTcca < 1:1057555/76‑1 (MQ=255) tttAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTccagc > 1:6038488/1‑76 (MQ=255) tttAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTccagc > 2:1758031/1‑76 (MQ=255) ttAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTccagcc > 1:6059534/1‑76 (MQ=255) ttAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTccagcc > 1:1489501/1‑76 (MQ=255) ttAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTccagcc > 1:4185018/1‑76 (MQ=255) ttAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTccagcc > 1:2874883/1‑76 (MQ=255) tAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTccagcca > 1:434529/1‑76 (MQ=255) tAGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTccagcca > 1:3735762/1‑76 (MQ=255) aGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTccagccag > 2:4259952/1‑76 (MQ=255) aGTTATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTccagccag < 2:4983143/76‑1 (MQ=255) ttATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGAc > 1:2380074/1‑76 (MQ=255) ttATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGAc > 2:1399426/1‑76 (MQ=255) tATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACt > 1:212029/1‑76 (MQ=255) tATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACt > 2:1794453/1‑76 (MQ=255) tATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACt > 1:4264486/1‑76 (MQ=255) tATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACt > 2:1035200/1‑76 (MQ=255) tATCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACt < 1:6163098/76‑1 (MQ=255) aTCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTa > 2:5866287/1‑76 (MQ=255) aTCCTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTa > 2:3192976/1‑76 (MQ=255) cTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACa > 2:231346/1‑76 (MQ=255) cTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACa > 2:5042689/1‑76 (MQ=255) cTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACa > 2:2871748/1‑76 (MQ=255) cTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACa > 1:3154117/1‑76 (MQ=255) cTTATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACa < 1:2773696/76‑1 (MQ=255) ttATTCGTTTAACAGCAAACCGGGCCCCCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACaa < 1:480236/76‑1 (MQ=255) ttATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACaa < 1:3543947/76‑1 (MQ=255) ttATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACaa > 1:4480890/1‑76 (MQ=255) ttttCTTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACaaa < 2:6144754/74‑1 (MQ=255) tATTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACaaa < 1:752561/76‑1 (MQ=255) aTTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACaaaa < 2:223852/76‑1 (MQ=255) aTTCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACaaaa > 1:3355660/1‑76 (MQ=255) ttCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAAt > 1:2608946/1‑76 (MQ=255) tCGTTTAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATc < 1:2047720/76‑1 (MQ=255) tttAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATCCGGCCTCGTCCAGCCAGCTAAACAAAATGGCt > 2:2795799/1‑76 (MQ=255) ttAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCtt < 2:857710/76‑1 (MQ=255) ttAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCtt < 2:4005365/76‑1 (MQ=255) ttAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTACAGCCAGACTAACAAAATCGCtt > 2:3558018/1‑76 (MQ=255) tAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTc > 2:4335659/1‑76 (MQ=255) tAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTc > 2:359132/1‑76 (MQ=255) tAACAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTc > 2:2077035/1‑76 (MQ=255) aaCAGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTCg > 1:5593799/1‑76 (MQ=255) aGCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTCGAgg > 2:4593929/1‑76 (MQ=255) gCAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTCGAGGa > 2:3986255/1‑76 (MQ=255) cAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTCGAGGAt > 2:1260064/1‑76 (MQ=255) cAAAACGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTCGAGGAt > 2:4180199/1‑76 (MQ=255) cAAAACGGCCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTCGAGGAt < 1:3415436/76‑1 (MQ=255) aaaaCGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTCGAGGAtt > 2:1900807/1‑76 (MQ=255) aaaaCGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTCGAGGAtt > 2:4758426/1‑76 (MQ=255) aaaCGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTCGAGGAttt > 1:5327276/1‑76 (MQ=255) aaaCGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTCGAGGAttt > 2:4383491/1‑76 (MQ=255) aaaCGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTCGAGGAttt < 1:3068847/76‑1 (MQ=255) aaaCGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTCGAGGAttt > 2:2279379/1‑76 (MQ=255) aaCGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTCGAGGAtttt < 2:6059534/76‑1 (MQ=255) aaCGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTCGAGGAtttt > 2:867979/1‑76 (MQ=255) aCGGGCCACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTCGAGGATTTtc > 1:5468830/1‑76 (MQ=255) | GTTGGGTAGAGAGGGTAATTTTCATCGCTTCTGTCATTTTAGTTATCCTTATTCGTTTAACAGCAAAACGGGCTACCCCAGGCAGCCCGTTTATTCATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTCGAGGATTTTCT > REL606/2577141‑2577284 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |