| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,011,220 | T→C | intergenic (+191/+47) | ECB_02811 → / ← ECB_02812 | conserved hypothetical protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,011,220 | 0 | T | C | 97.7% | 544.1 / ‑0.3 | 132 | intergenic (+191/+47) | ECB_02811/ECB_02812 | conserved hypothetical protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (70/59); total (72/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
Only 100 of 157 total aligned reads displayed.
CCGAGTCCGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAATAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGTTAACAAAA > REL606/3011147‑3011292 | ccGAGTCCGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATataaaacaa < 2:4496672/76‑1 (MQ=255)ccGAGTCCGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATataaaacaa > 1:3403466/1‑76 (MQ=255) cGAGTCCGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATataaaacaaa > 2:2593702/1‑76 (MQ=255) cGAGTCCGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATataaaacaaa < 2:1503934/76‑1 (MQ=255) cGAGTCCGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATataaaacaaa > 1:3774515/1‑76 (MQ=255) cGAGTCCGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATataaaacaaa > 1:2557532/1‑76 (MQ=255) aGTCCGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACt < 2:3440952/76‑1 (MQ=255) gTCCGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACtt > 2:6108962/1‑76 (MQ=255) gTCCGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACtt < 1:956316/76‑1 (MQ=255) gTCCGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACtt > 2:4212549/1‑76 (MQ=255) cGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTggg > 1:4255114/1‑76 (MQ=255) cGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTggg > 2:1835742/1‑76 (MQ=255) caccacTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGc < 1:3965514/76‑1 (MQ=255) ccacTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCAt < 1:977980/76‑1 (MQ=255) cacTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATa < 1:3776350/76‑1 (MQ=255) acTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATaa > 2:1115214/1‑76 (MQ=255) cTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAAc < 2:1019409/76‑1 (MQ=255) tAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACg < 2:1638303/76‑1 (MQ=255) aaTTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGc > 1:4393092/1‑76 (MQ=255) aaTTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGc > 1:3912056/1‑76 (MQ=255) aTTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGcc > 2:334837/1‑76 (MQ=255) ttCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCa > 1:5962624/1‑76 (MQ=255) ttCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCa < 1:6227428/76‑1 (MQ=255) tCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCAt > 1:4998194/1‑76 (MQ=255) tCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCAt < 2:1495859/76‑1 (MQ=255) tCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCAt < 1:1437891/76‑1 (MQ=255) ttAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCa < 2:454172/76‑1 (MQ=255) ttAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCa < 1:2577069/76‑1 (MQ=255) ttAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCa > 2:2462474/1‑76 (MQ=255) ttAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCa > 2:3342892/1‑76 (MQ=255) tAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAg > 2:1989045/1‑76 (MQ=255) aaGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAgg < 2:5161470/76‑1 (MQ=255) aaGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAgg > 2:4952995/1‑76 (MQ=255) aaGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAgg > 2:2321608/1‑76 (MQ=255) aaGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAgg < 1:5924488/76‑1 (MQ=255) aGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGt > 2:3057584/1‑76 (MQ=255) aGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGt > 2:75356/1‑76 (MQ=255) ccGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTaa > 1:4762291/1‑76 (MQ=255) cGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTaaa > 2:4668405/1‑76 (MQ=255) cGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTaaa < 1:2987293/76‑1 (MQ=255) acaAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTc < 2:184480/76‑1 (MQ=255) aaGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTccc > 2:2259172/1‑76 (MQ=255) aaGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTccc > 2:2275333/1‑76 (MQ=255) aaGGCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTccc > 1:2845575/1‑76 (MQ=255) gCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGaa < 1:1095000/76‑1 (MQ=255) gCGGGTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGaa < 2:5351556/76‑1 (MQ=255) gggTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAatc < 1:5093468/76‑1 (MQ=255) gggTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAatc < 1:2926637/76‑1 (MQ=255) ggTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAatca < 2:5790408/76‑1 (MQ=255) gTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAatcat > 2:156680/1‑76 (MQ=255) gTTTTTGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAatcat > 2:3671026/1‑76 (MQ=255) ttttGCTTTTGGATCTTTATATAAAATAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAAtcatca > 1:4055598/1‑76 (MQ=255) ttttGCTTTTGGATCTTTATATAAAATAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAAtcatca > 2:1261285/1‑76 (MQ=255) ttttGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAAtcatca > 1:413923/1‑76 (MQ=255) ttttGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAAtcatca > 1:185113/1‑76 (MQ=255) tttGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCaa < 1:4405199/76‑1 (MQ=255) tGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATa > 2:2074886/1‑76 (MQ=255) tGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATa > 2:6076903/1‑76 (MQ=255) tGCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATa > 1:3328713/1‑76 (MQ=255) gCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACCCTCCCGAATCATCAATaa < 1:4604735/76‑1 (MQ=255) gCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATaa > 2:5718348/1‑76 (MQ=255) gCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATaa < 2:984791/76‑1 (MQ=255) gCTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATaa < 1:4124400/76‑1 (MQ=255) cTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATaaa < 2:783467/76‑1 (MQ=255) cTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATaaa > 2:6262763/1‑76 (MQ=255) cTTTTGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATaaa < 1:1467805/76‑1 (MQ=255) ttttGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAAc < 1:1115214/76‑1 (MQ=255) ttttGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAAc > 2:6285156/1‑76 (MQ=255) ttttGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAAc > 1:2670569/1‑76 (MQ=255) tttGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACa > 2:4442181/1‑76 (MQ=255) ttGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACAt < 1:2266742/76‑1 (MQ=255) ttGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACAt < 1:36937/76‑1 (MQ=255) ttGGATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACAt < 1:3592520/76‑1 (MQ=255) ggATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATcc > 1:4517301/1‑76 (MQ=255) gATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATccc < 1:5058357/76‑1 (MQ=255) gATCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATccc > 1:3675771/1‑76 (MQ=255) aTCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATcccc > 1:1600457/1‑76 (MQ=255) aTCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATcccc < 2:2909249/76‑1 (MQ=255) tCTTTATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATccccc < 1:5545563/76‑1 (MQ=255) ttATATAAAATAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGtt < 2:1291168/76‑1 (MQ=255) ttATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGtt > 1:1760032/1‑76 (MQ=255) ttATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGtt > 1:2458617/1‑76 (MQ=255) ttATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGtt < 2:430612/76‑1 (MQ=255) ttATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGtt < 2:400870/76‑1 (MQ=255) ttATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGtt > 1:4183911/1‑76 (MQ=255) ttATATAAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGtt > 1:4186645/1‑76 (MQ=255) tatataAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGTTa > 1:5587262/1‑76 (MQ=255) tatataAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGTTa > 1:4776114/1‑76 (MQ=255) atataAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGTTaa < 2:4857814/76‑1 (MQ=255) atataAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGTTaa > 1:1170768/1‑76 (MQ=255) atataAAACAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGTTaa < 1:4601698/76‑1 (MQ=255) ataaaacaaaCTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGTTAACa > 1:2453282/1‑76 (MQ=255) ataaaacaaaCTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGTTAACa < 1:4204888/76‑1 (MQ=255) ataaaacaaaCTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGTTAACa < 1:1210973/76‑1 (MQ=255) taaaacaaaCTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGTTAACaa < 1:902291/76‑1 (MQ=255) taaaacaaaCTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGTTAACaa > 1:3307564/1‑76 (MQ=255) taaaacaaaCTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGTTAACaa > 1:1913541/1‑76 (MQ=255) taaaacaaaCTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGTTAACaa < 2:4053754/76‑1 (MQ=255) aaacaaacTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGTTAACaaaa < 2:5476288/76‑1 (MQ=255) aaacaaacTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGTTAACaaaa < 2:1979126/76‑1 (MQ=255) | CCGAGTCCGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTTTATATAAAATAAACTTGGGAGCATAACGCCATCAGGTCTTAAACACTCCCGAATCATCAATAAACATCCCCCGTTAACAAAA > REL606/3011147‑3011292 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |