| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,145,830 | A→G | intergenic (+161/‑34) | dnaG → / → rpoD | DNA primase/RNA polymerase sigma factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,145,830 | 0 | A | G | 99.4% | 684.8 / ‑4.1 | 176 | intergenic (+161/‑34) | dnaG/rpoD | DNA primase/RNA polymerase sigma factor |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (77/98); total (77/99) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.68e-01 | |||||||||||
Only 100 of 193 total aligned reads displayed.
AATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTC > REL606/3145757‑3145902 | aaTTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCCACGcc > 2:2155495/1‑76 (MQ=255)aaTTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGcc > 1:4102227/1‑76 (MQ=255)aaTTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGcc > 1:3330448/1‑76 (MQ=255) aTTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCCACGCCa > 2:2061101/1‑76 (MQ=255) aTTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCa > 1:1432641/1‑76 (MQ=255) aTTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAAACGCCCAACGccc > 2:4858928/1‑75 (MQ=255) ttATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCaa < 1:4440897/76‑1 (MQ=255) ttATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCaa > 1:5263777/1‑76 (MQ=255) ttATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCaa < 1:6057170/76‑1 (MQ=255) ttATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCaa > 1:2440728/1‑76 (MQ=255) tATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTGAATCGCCCAAAGCCAAc > 1:3128815/1‑76 (MQ=255) tATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAAc < 1:1273400/76‑1 (MQ=255) tCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCt > 1:3763594/1‑76 (MQ=255) tCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCt > 1:5536758/1‑76 (MQ=255) tCGTTGGCGGTAAACAACCGTGGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCt < 2:4254869/76‑1 (MQ=255) cGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTc < 1:1095237/76‑1 (MQ=255) cGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTc > 1:2197722/1‑76 (MQ=255) gTTGGCGTAAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGCCATCGGGTCAATCGCCCAACGCCAACCTCa < 2:591615/76‑1 (MQ=255) gTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCa < 2:2749286/76‑1 (MQ=255) gTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGGCAATCGCCCAACGCCAACCTCa > 2:4193536/1‑76 (MQ=255) ttGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCCACGCCAACCTCAt > 1:1600447/1‑76 (MQ=255) ttGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCAt < 1:1092926/76‑1 (MQ=255) tGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATg < 1:196032/76‑1 (MQ=255) tGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATg < 1:3482419/76‑1 (MQ=255) ggCGGTAAACAACCGTTGGATTTCAGCGTTAACTGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGa < 1:757340/76‑1 (MQ=255) ggCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGa < 1:3700513/76‑1 (MQ=255) cGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGaaa > 1:122607/1‑76 (MQ=255) cGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGaaa < 1:4254002/76‑1 (MQ=255) ggTAAACAACCGTTGGATTTCAGCTTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAAt < 1:530774/76‑1 (MQ=255) gTAAACAGCCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATa < 2:49072/76‑1 (MQ=255) gTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATa > 1:5084496/1‑76 (MQ=255) tAAACAACCGTTGGATTTCAGCGTTAACGGCTGGAGGAGATCGGGGCCATCGCCCCACACCCAACACATGAAATaa > 2:4945919/1‑76 (MQ=255) tAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATaa < 2:1791645/76‑1 (MQ=255) tAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATaa < 2:3178052/76‑1 (MQ=255) tAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATaa < 2:3829271/76‑1 (MQ=255) aaaCAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAg > 2:539580/1‑76 (MQ=255) aacaacCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAgt < 1:4662545/76‑1 (MQ=255) acaacCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAgtg > 1:5156059/1‑76 (MQ=255) caacCGTTGGATTTCAGCGTTAACGGCTGGAGGACATCGGGTGAATCGCCCAACGCCAACCTCATGAAATAAgtgt > 2:909837/1‑76 (MQ=255) caacCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAgtgt < 2:3128815/76‑1 (MQ=255) caacCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGACAATCGCCCAACGCCAACCTCATGAAATAAgggt > 1:3557684/1‑76 (MQ=255) aacCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGtgtg < 1:2941390/76‑1 (MQ=255) acCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTgg > 2:5344503/1‑76 (MQ=255) cGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGAt > 1:2085013/1‑76 (MQ=255) cGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGGCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGaa > 2:4729770/1‑75 (MQ=255) gTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATa < 2:778496/76‑1 (MQ=255) tGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATAcc > 1:3470072/1‑76 (MQ=255) tGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATAcc < 2:1358119/76‑1 (MQ=255) gATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGt < 2:3618815/76‑1 (MQ=255) gATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGt > 1:3638035/1‑76 (MQ=255) gATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATAACGt > 1:4835032/1‑76 (MQ=255) ttCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACGGTCtt < 2:3557684/76‑1 (MQ=255) ttCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCtt < 2:3579198/76‑1 (MQ=255) ttCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCtt < 1:4354304/76‑1 (MQ=255) tCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTa > 1:3196675/1‑76 (MQ=255) tCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTa < 1:3865693/76‑1 (MQ=255) cAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTAt > 1:4743354/1‑76 (MQ=255) gCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATgg < 1:1565729/76‑1 (MQ=255) gCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATgg < 2:2592794/76‑1 (MQ=255) cGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATAACGTCTTAttgg > 1:1633000/1‑73 (MQ=255) gTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAg > 1:3038274/1‑76 (MQ=255) gTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAg < 1:2142811/76‑1 (MQ=255) ttAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGc < 1:862022/76‑1 (MQ=255) ttAACGGCTGAAGGACATCGGGCCAATCGCCCAACGCCAACCTCATAAAATAAGTGTGGATACCGTCTTTTGGAGc > 1:267311/1‑76 (MQ=255) ttAACGGCTGAAAGACACGGGGTCAATCGGCCAACGCCAACCTCATGAAATAAGTGGGGAGACCCGCTTTTGGTGc > 2:3302701/1‑76 (MQ=255) tAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCa < 1:6261543/76‑1 (MQ=255) aaCGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCaa < 1:2541277/76‑1 (MQ=255) aCGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCaaa < 2:5475659/76‑1 (MQ=255) aCGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCaaa < 2:4361029/76‑1 (MQ=255) ggCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAAc < 1:4482323/76‑1 (MQ=255) ggCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAAc < 2:3588364/76‑1 (MQ=255) ggCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAAc < 1:6179355/76‑1 (MQ=255) gCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAAcc > 2:4619093/1‑76 (MQ=255) gCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCCTCTTATGGAGCAAAAcc > 2:1138978/1‑76 (MQ=255) tGAAGGACATCGGGTCAGTCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCg > 1:132667/1‑76 (MQ=255) tGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCg > 2:398642/1‑76 (MQ=255) aaGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCa > 1:1811629/1‑76 (MQ=255) aaGGACAGTGGGTCAATCGCCCAACGCCAGCCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCa < 2:5614460/76‑1 (MQ=255) aGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAg > 1:2556708/1‑76 (MQ=255) aGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAg < 2:3328014/76‑1 (MQ=255) ggACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGt > 1:57711/1‑76 (MQ=255) gACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTc > 1:4756699/1‑76 (MQ=255) gACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTc < 1:2049022/76‑1 (MQ=255) aTCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTcaca < 1:9703/76‑1 (MQ=255) cGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGGCACAGc > 2:1609549/1‑76 (MQ=255) ggTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCCCAGTCACAGCTg > 2:2140373/1‑76 (MQ=255) tCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGaa < 2:6281350/76‑1 (MQ=255) tCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGaa > 1:381630/1‑76 (MQ=255) tCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGaa < 1:4193536/76‑1 (MQ=255) aTCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCCGTCACAGCTGAAAct > 1:2747108/1‑76 (MQ=255) aTCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAAct < 1:1504932/76‑1 (MQ=255) cGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGGGCAAAAACCGCAGTCACAGCTGAAActtc > 2:2800316/1‑76 (MQ=255) cccAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAActtctt > 1:2038278/1‑76 (MQ=255) cccAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAActtctt < 2:1966356/76‑1 (MQ=255) cccAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAActtctt < 2:2555510/76‑1 (MQ=255) ccAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGGGCAAAACCCGCAGTCACAGCTGAAActtcttt > 2:555271/1‑75 (MQ=255) ccAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTg < 2:4785989/76‑1 (MQ=255) cAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGt < 2:1169815/76‑1 (MQ=255) aaCGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTc > 2:5206409/1‑76 (MQ=255) aaCGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTc > 2:2946370/1‑76 (MQ=255) | AATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTC > REL606/3145757‑3145902 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |