| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,340,143 | G→A | P200P (CCG→CCA) | yhdJ → | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,340,143 | 0 | G | A | 98.4% | 731.4 / 0.9 | 185 | P200P (CCG→CCA) | yhdJ | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (98/84); total (100/85) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01 | |||||||||||
Only 100 of 192 total aligned reads displayed.
GGGTAACGTCTGGGATTTTCCGCGCGTGCGTTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCCGGT > REL606/3340071‑3340215 | gggTANNGTCTGGGATTTTCCGCGCGTGCGTTATTTAATGGATGAATATGAGAACCACCCGACGCAAANACCAGaa > 2:1067833/1‑76 (MQ=255) ggTACCGTCTGGGATTTCCCGCGCGTGCGTTATTTAATGGATAAATATGAGAACCACCCGACGCAAAAACCAGAAg < 1:1772848/76‑1 (MQ=255) ggTAACGTCTGGGATTTTCCGCGCGTGCGTTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAg > 2:1871400/1‑76 (MQ=255) tAACGTCTGGGATTTTCCGCGCGTGCGTTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGcc > 2:37350/1‑76 (MQ=255) aaCGTCTGGGATTTTCCGCGCGTGCGTTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCt > 1:1007738/1‑76 (MQ=255) aCGTCTGGGATTTTCCGCGCGTGCGTTATTTAATGGATGAATATGAGAACCCCCCGACGCAAAAACCAGAAGCCtt < 2:4691802/76‑1 (MQ=255) cGTCTGGGATTTTCCGCGCGTGCGTTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTa > 2:5236335/1‑76 (MQ=255) cGTCTGGGATTTTCCGCGCGTGCGTTATTTAATGAAACAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTa < 1:2761842/76‑1 (MQ=255) tcggggATTTTCCGCGCGTGCGTTATTTAATGGATGAATATGAGAACCCCCCGACGCAAAAACCAGAAGCCTTACt < 2:2571208/73‑1 (MQ=255) tCTGGGATTTTCCGCGCGTGCGTTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACt > 1:2540784/1‑76 (MQ=255) cTGGGATTTTCCGCGCGTGCGTTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTg < 1:5598796/76‑1 (MQ=255) cTGGGATTTTCCGCGCGTGCGTTATTTAAGGGATAAATATGAGAACCCCCCGACGCAAAAACCAGAAGCCTTACTg < 2:1217401/76‑1 (MQ=255) ggggTTTTCCGCGCGTGCGTTATTTAAGGGATGAATATGAGAACCCCCCGACGCAAAAACCAGAAGCCTTACTGaa < 1:1679436/76‑1 (MQ=255) gggATTTTCCGCGCGTGCGTTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGaa > 2:1722826/1‑76 (MQ=255) gATTTTCCGCGCGTGCGTTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAAc < 2:2972888/76‑1 (MQ=255) aTTTTCCGCGCGTGCGTTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACg > 1:5890930/1‑76 (MQ=255) aTTTTCCGCGCGTGCGTTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACg < 1:4579906/76‑1 (MQ=255) aTTTTCCGCGCGTGCGTTATTTAATGGATGAATATGAAAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACg < 2:4738694/76‑1 (MQ=255) aTTTTCCGCACGTGCTTTATTTAATGTAGAAATAGGAGAACCCCCCGACGCAAAAACCAGAAGCCTTACTGAAACg < 1:3138799/76‑1 (MQ=255) ttttCCGCGCGTGCGTTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGc > 1:613667/1‑76 (MQ=255) ttttCCGCGCGTGCGTTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGc > 1:297386/1‑76 (MQ=255) ttCCGCGCGTGCGTTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCat > 1:3542189/1‑76 (MQ=255) ccGCGCGTGCGTTATTTAATGGATGAATATGAGAACCCCCCGACGCAAAAACCAGAAGCCTTACTGAAACGCatta < 2:3966330/76‑1 (MQ=255) ccGCGCGTGCGTTATTTAAGGGATGAATATGAAACCCCCCCGACGCAAAAACCAGAAGCCTTACTGAAACGCatta < 2:2790998/76‑1 (MQ=255) cgcgcgTGCGTTATTTAATGGATGAATATGAGAACCCCCCGACGCAAAAACCAGAAGCCTTACTGAAACGCattat < 2:3988662/76‑1 (MQ=255) cgcgcgTGCGTTATTTAATGGATAAATATGAGAACCCCCCGACGCAAAAACCAGAAGCCTTACTGAAACGCattat < 1:5757404/76‑1 (MQ=255) cgtgcgtTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATtctc < 2:5649853/76‑1 (MQ=255) cgtgcgtTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATtctc > 2:2181675/1‑76 (MQ=255) gtgcgtTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCg > 2:1309987/1‑76 (MQ=255) gtgcgtTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCg > 1:5609718/1‑76 (MQ=255) tgcgtTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGc > 1:5237302/1‑76 (MQ=255) cgtTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCct > 1:3035628/1‑76 (MQ=255) cgtTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCct > 1:2903552/1‑76 (MQ=255) cgtTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCct > 1:4485157/1‑76 (MQ=255) cgtTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCct > 2:3801232/1‑76 (MQ=255) gtTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCctc > 2:5863464/1‑76 (MQ=255) ttATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCctct > 1:471888/1‑76 (MQ=255) tATTTAATTGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCtt > 1:1820212/1‑76 (MQ=255) tATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCtt > 1:862414/1‑76 (MQ=255) tATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCtt > 2:1642055/1‑76 (MQ=255) aTTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTc > 2:3068879/1‑76 (MQ=255) aTTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTc > 1:3443791/1‑76 (MQ=255) aTTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTc > 2:4655548/1‑76 (MQ=255) aTTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTc > 2:1713024/1‑76 (MQ=255) aTTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTc > 2:4133560/1‑76 (MQ=255) aTTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTc > 1:428039/1‑76 (MQ=255) aTTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTc > 1:522876/1‑76 (MQ=255) tttAATGGATGAATATGAGAACCCCCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTcc < 2:4453426/76‑1 (MQ=255) tttAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTcc > 1:1375716/1‑76 (MQ=255) tttAAGGGATGAATATGAGACCCCCCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTcc < 2:320050/76‑1 (MQ=255) tttAAGGGATGAATATGAGAACCCCCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTcc < 2:5989434/76‑1 (MQ=255) tttAAGGGAGGAATATGAGAACCCCCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTcc < 2:644113/76‑1 (MQ=255) ttaaaTGGATGAATATGAGAACCCCCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTcc < 2:741356/73‑1 (MQ=255) ttAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCa > 1:5670646/1‑76 (MQ=255) ttAATGGATGAATAGGAGACCCCCCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCa < 1:1389119/76‑1 (MQ=255) ttAAGGGATGAATATGAAAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCa < 2:1367877/76‑1 (MQ=255) tAATGGATGAATATGAGACCCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCaa < 2:2024814/76‑1 (MQ=255) tAATGGATGAATATGAGAACCCCCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCaa < 1:2687573/76‑1 (MQ=255) tAATGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCaa > 1:4274284/1‑76 (MQ=255) aaTGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAAc > 2:4809083/1‑76 (MQ=255) aaTGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAAc > 2:2895712/1‑76 (MQ=255) aTGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGACACGCATTATTCTCGCCTCTTCCAAcc > 2:1413223/1‑76 (MQ=255) aTGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAAcc > 2:5730484/1‑76 (MQ=255) tGGATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAAccc > 1:3774741/1‑76 (MQ=255) tGGATGAATATGAAACCCCCCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAAccc < 2:3928607/76‑1 (MQ=255) ggATGAATATGAGAACCCCCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCa < 2:594485/76‑1 (MQ=255) ggATGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCa > 1:1584278/1‑76 (MQ=255) gaggAATAGGAAAACCCCCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAg < 2:1954416/73‑1 (MQ=255) gATGAATAGGAGACCCCCCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAg < 1:733359/76‑1 (MQ=255) aTGAATATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAgg > 2:4060594/1‑76 (MQ=255) aaTATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGa > 2:618459/1‑76 (MQ=255) aaTATGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGa > 1:962800/1‑76 (MQ=255) atagGAGACCCCCCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGat < 1:2133831/76‑1 (MQ=255) tatGAGACCCCCCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGata < 2:2076305/76‑1 (MQ=255) tatGAGAACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGata > 2:1503452/1‑76 (MQ=255) atGAGACCCCCCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGatat < 2:4341459/76‑1 (MQ=255) aggagaCCCCCCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGatat < 1:3732986/74‑1 (MQ=255) gaagaCCCCCCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATc < 1:537273/74‑1 (MQ=255) agaACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGt > 1:5107727/1‑76 (MQ=255) agaACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGt > 2:2322684/1‑76 (MQ=255) gaACCACCCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGtt > 1:5055866/1‑76 (MQ=255) acccccCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTtct < 1:1151676/76‑1 (MQ=255) acccccCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTtct < 1:1433375/76‑1 (MQ=255) accaccCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTtct > 2:5530038/1‑76 (MQ=255) ccccccGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTtctc < 1:4025952/73‑1 (MQ=255) ccaccCGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTtctc > 1:4740679/1‑76 (MQ=255) cccccGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTTCTCg < 2:5370004/74‑1 (MQ=255) cccGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTTCTCGAc > 1:394074/1‑76 (MQ=255) cGACGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTTCTCGAccc < 1:4923358/76‑1 (MQ=255) cGCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGtt < 2:4938271/76‑1 (MQ=255) gCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGttt < 2:3396841/76‑1 (MQ=255) gCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGttt > 1:905648/1‑76 (MQ=255) gCAAAAACCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGttt < 2:4246023/76‑1 (MQ=255) aaaaaCCAGAAGCCTTACTAAAACGAATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGc < 1:4216483/76‑1 (MQ=255) aaaaCCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGcc > 2:255732/1‑76 (MQ=255) aaaCCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCCg < 2:1268125/76‑1 (MQ=255) aaaCCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCCg > 2:4921533/1‑76 (MQ=255) aaaCCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCCg > 2:4423265/1‑76 (MQ=255) ctgggAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCCGGt < 1:805121/73‑1 (MQ=255) aCCAGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCCGGt < 1:2430464/76‑1 (MQ=255) | GGGTAACGTCTGGGATTTTCCGCGCGTGCGTTATTTAATGGATGAATATGAGAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCCGGT > REL606/3340071‑3340215 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |