| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,489,214 | T→A | intergenic (‑8/+9) | glpR ← / ← glpG | DNA‑binding transcriptional repressor/predicted intramembrane serine protease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,489,214 | 0 | T | A | 99.0% | 797.6 / ‑5.4 | 210 | intergenic (‑8/+9) | glpR/glpG | DNA‑binding transcriptional repressor/predicted intramembrane serine protease |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base A (115/92); total (116/94) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 215 total aligned reads displayed.
GGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCG > REL606/3489141‑3489286 | tACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCt < 1:36698/76‑1 (MQ=255) tACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCt > 2:5364633/1‑76 (MQ=255) aCTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTg > 1:4324657/1‑76 (MQ=255) cTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTgg > 1:5632924/1‑76 (MQ=255) cTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTgg < 2:3724201/76‑1 (MQ=255) tGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGa > 1:1708470/1‑76 (MQ=255) gACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGaa < 1:3617946/76‑1 (MQ=255) cATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAatt < 2:1526808/76‑1 (MQ=255) tAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAattat < 2:6211886/76‑1 (MQ=255) aCCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTAttt > 2:756307/1‑76 (MQ=255) aCCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTAttt > 2:5452947/1‑76 (MQ=255) aCCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTAttt < 2:2915186/76‑1 (MQ=255) ccTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTAttttc < 1:5944332/76‑1 (MQ=255) ccTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTAttttc < 2:1954612/76‑1 (MQ=255) ctgctgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTAttttcg < 2:4736239/76‑1 (MQ=255) ctgctgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTAttttcg > 1:5467550/1‑76 (MQ=255) tgctgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTAttttcgt > 1:2610855/1‑76 (MQ=255) gctgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTAttttcgtt > 2:2850188/1‑76 (MQ=255) ctgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTAttttcgttt < 1:3346648/76‑1 (MQ=255) gTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTAttttcgttttc > 2:2573485/1‑76 (MQ=255) gTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTAttttcgttttc > 1:3107271/1‑76 (MQ=255) gTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTAttttcgttttc > 2:5556798/1‑76 (MQ=255) tttAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTAttttcgttttcg > 2:1919238/1‑76 (MQ=255) tttAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTAttttcgttttcg > 1:4610468/1‑76 (MQ=255) tttAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTAttttcgttttcg > 2:4501161/1‑76 (MQ=255) ttAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTcgc > 1:3017049/1‑76 (MQ=255) tAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTcgcg > 2:4009701/1‑76 (MQ=255) tAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTcgcg < 2:3552508/76‑1 (MQ=255) tAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTcgcg < 2:4788336/76‑1 (MQ=255) aaCCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCgcgc > 1:339217/1‑76 (MQ=255) aaCCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCgcgc > 2:4490568/1‑76 (MQ=255) aCCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCa < 2:1180690/76‑1 (MQ=255) ccAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCAt < 2:1402004/76‑1 (MQ=255) aGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTg < 1:5248938/76‑1 (MQ=255) aGTTCGATAATACCGTTGTGACGTTGTATTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTg > 1:3265108/1‑76 (MQ=255) ttCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGTGCATTGAg > 2:5850651/1‑76 (MQ=255) ttCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAg > 1:1756556/1‑76 (MQ=255) cGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCg > 1:1194744/1‑76 (MQ=255) cGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCg < 1:3178176/76‑1 (MQ=255) gATAATACCGTTGTTACGGTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGa < 1:4717242/76‑1 (MQ=255) ataataCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGaa > 1:1882058/1‑76 (MQ=255) ataataCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGaa < 2:224662/76‑1 (MQ=255) taataCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt < 1:4465228/76‑1 (MQ=255) taataCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt < 2:597331/76‑1 (MQ=255) aataCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATc > 1:3173612/1‑76 (MQ=255) ataCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATca < 1:1662883/76‑1 (MQ=255) taCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaa < 1:4899259/76‑1 (MQ=255) taCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaa > 2:6260336/1‑76 (MQ=255) aCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaac > 2:3172115/1‑76 (MQ=255) ccGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaaca > 1:4244566/1‑76 (MQ=255) cGTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaacaa > 1:1725090/1‑76 (MQ=255) gTTGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaa < 1:115668/76‑1 (MQ=255) ttGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaaa < 2:3353345/76‑1 (MQ=255) ttGTGACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaaa < 1:2149194/76‑1 (MQ=255) tgtgACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAg > 1:6156339/1‑76 (MQ=255) tgtgACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAg < 2:4611730/76‑1 (MQ=255) tgtgACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAg < 2:3569971/76‑1 (MQ=255) tgtgACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAg > 1:3932384/1‑76 (MQ=255) tgACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc > 1:1405005/1‑76 (MQ=255) tgACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc > 1:2838247/1‑76 (MQ=255) gACGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCa > 1:5337968/1‑76 (MQ=255) aCGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCAt < 1:4408047/76‑1 (MQ=255) aCGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCAt > 1:992943/1‑76 (MQ=255) cGTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATc < 2:3861994/76‑1 (MQ=255) gTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCg > 2:2786568/1‑76 (MQ=255) gTTGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCg < 1:4810808/76‑1 (MQ=255) ttGTGTTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGc > 1:4659617/1‑76 (MQ=255) gtgtTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTa > 2:2505093/1‑76 (MQ=255) gtgtTTGTTTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTa < 2:3195032/76‑1 (MQ=255) gtgtTTGTTTCATTCCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTa > 1:83253/1‑76 (MQ=255) tgtttgttTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTaa < 1:6245004/76‑1 (MQ=255) tgtttgttTCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTaa < 1:2405238/76‑1 (MQ=255) gtttgtttCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTaaa > 1:3865270/1‑76 (MQ=255) gtttgtttCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTaaa > 2:1760571/1‑76 (MQ=255) tttgtttCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAc > 1:1290306/1‑76 (MQ=255) tttgtttCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAc < 2:4900711/76‑1 (MQ=255) tttgtttCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAc > 1:3572685/1‑76 (MQ=255) tgtttCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAccc > 1:1739334/1‑76 (MQ=255) gtttCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCa < 2:2103241/76‑1 (MQ=255) gtttCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCa < 2:5632924/76‑1 (MQ=255) tttCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAACCCCAc < 1:80427/76‑1 (MQ=255) ttCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACg < 2:2693830/76‑1 (MQ=255) tCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACgg < 2:819477/76‑1 (MQ=255) tCATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACgg > 1:780637/1‑76 (MQ=255) cATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGc > 1:1446240/1‑76 (MQ=255) cATTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGc > 2:5300492/1‑76 (MQ=255) aTTTCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCt < 1:1049312/76‑1 (MQ=255) tttCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTa > 1:6270927/1‑76 (MQ=255) tttCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTa < 2:4393297/76‑1 (MQ=255) tttCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTa > 2:3265093/1‑76 (MQ=255) tgtCTAAAACCCTTGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTa < 2:5399201/74‑1 (MQ=255) tCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAc < 1:5443038/76‑1 (MQ=255) tCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAc > 1:4192941/1‑76 (MQ=255) tCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAc > 1:2882719/1‑76 (MQ=255) tCTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAc > 2:1356745/1‑76 (MQ=255) cTAAAACCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAcc > 1:1519629/1‑76 (MQ=255) aaaaCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCg > 2:5103902/1‑76 (MQ=255) aaaaCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCg > 2:1110869/1‑76 (MQ=255) aaaaCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCg > 1:2570645/1‑76 (MQ=255) aaaaCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCg < 1:1611919/76‑1 (MQ=255) | GGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCG > REL606/3489141‑3489286 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |