BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,517,709	T→C	Y31H (TAT→CAT)	yhhA →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,517,709	0	T	C	100.0%	732.3 / NA	182	Y31H (TAT→CAT)	yhhA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (121/61); total (121/61)

Only 100 of 190 total aligned reads displayed.

TCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGGATGCTG  >  REL606/3517636‑3517780

tCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCAt                                                                       >  2:4481017/1‑76 (MQ=255)

 cTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATc                                                                      >  1:3310423/1‑76 (MQ=255)

 cTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATc                                                                      >  1:2441276/1‑76 (MQ=255)

  ttACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCa                                                                     >  2:2015261/1‑76 (MQ=255)

  ttACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCa                                                                     >  2:52115/1‑76 (MQ=255)

   tACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAg                                                                    >  1:2519628/1‑76 (MQ=255)

       gcccTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATCCTCTGAACAACCCTAC‑CCAGCCGGGGCATCAGATAc                                                                <  2:1620695/73‑1 (MQ=255)

        cACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATAcc                                                               >  1:938612/1‑76 (MQ=255)

         aCTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATAccc                                                              >  1:4629800/1‑76 (MQ=255)

           tCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACccag                                                            >  1:5955977/1‑76 (MQ=255)

           tCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACccag                                                            >  1:3055795/1‑76 (MQ=255)

              tGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACccagcca                                                         >  2:4831661/1‑76 (MQ=255)

               gctgTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACACCCCTAA‑CCAGCCGGGGCATCAGATACccagccag                                                        <  2:3204975/73‑1 (MQ=255)

               gCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACACCCCTAC‑CCAGCCGGGGCATCAGATACccagccag                                                        <  2:1315626/76‑1 (MQ=255)

               gCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACACCCCTAA‑CCAGCCGGGGCATCAGATACccagccag                                                        <  2:1207047/76‑1 (MQ=255)

               gCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACccagccag                                                        >  1:1113548/1‑76 (MQ=255)

               gCCGTTTGACGTCTTTGCACCCCCTATTAATACTCTGAACACCCCTAA‑CCAGCCGGGGCATCAGATACccagccag                                                        <  1:2568858/76‑1 (MQ=255)

                ccGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCcagccagc                                                       >  2:2981669/1‑76 (MQ=255)

                 cGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCcagca                                                      >  1:6046585/1‑76 (MQ=255)

                  gTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCCGCcagcag                                                     >  1:699900/1‑76 (MQ=255)

                  gTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCcagcag                                                     >  2:1727035/1‑76 (MQ=255)

                  gTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCcagcag                                                     >  1:4152710/1‑76 (MQ=255)

                  gTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCcagcag                                                     >  1:1031523/1‑76 (MQ=255)

                  ctttGTCGGCTTGGAACGCCCCATTAATTCTCAGAACACCCCTAC‑CCAGCCGGGGCATCAGATCCCCAGCcagcag                                                     <  1:5803420/75‑1 (MQ=255)

                   tttGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCATCCagcagc                                                    >  2:4975991/1‑76 (MQ=255)

                   tttGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCagcagc                                                    >  1:568661/1‑76 (MQ=255)

                   tttGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCagcagc                                                    >  1:4316848/1‑76 (MQ=255)

                   ttggTCGGTTTTGCACAGCCCATAAATACTCTGAACACCCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCagcagc                                                    <  2:6220545/73‑1 (MQ=255)

                    ttGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCg                                                   >  1:3687072/1‑76 (MQ=255)

                     tGTCGGCTTGGCACAGCCCATTAATCCTCTGAACACCCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCgg                                                  <  1:4378568/76‑1 (MQ=255)

                      gTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGa                                                 >  1:1481067/1‑76 (MQ=255)

                      gTCGCCTTGCCACAGCCAATTAATCCTCTGAACACCCCTAC‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGa                                                 <  1:3674080/76‑1 (MQ=255)

                       tCGGCTTGTCACACCCCATTAATACTCAGAACACCCCAAC‑CCAGCCGGGGCATCAGATCCCCAGCCAGCAGCGGAt                                                <  1:4753861/76‑1 (MQ=255)

                         ggttttGCCCAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGc                                              <  1:4445407/73‑1 (MQ=255)

                          gttttGCCCAGCCAATTAATCCTCTGAACACCCCAAC‑CCAGCCGGGGCATCAGATCCCCAGCCAGCAGCGGATGCa                                             <  2:2506736/74‑1 (MQ=255)

                          gCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCa                                             >  1:5345749/1‑76 (MQ=255)

                           cTTTGCCCAGCCCATAAATCCTCTGAACACCCCAAC‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCaa                                            <  1:4079764/76‑1 (MQ=255)

                            tttGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCaaa                                           >  1:2322309/1‑76 (MQ=255)

                               gCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAAccc                                        >  1:5502943/1‑76 (MQ=255)

                               gCACACCCAATAAATCCTCTAAACACCCCAAC‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAAccc                                        <  2:5305964/76‑1 (MQ=255)

                                cacgGCCCATTAATCCCCGAAAAACCCCTAC‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCa                                       <  1:2984524/76‑1 (MQ=255)

                                 acaGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAg                                      >  1:4965842/1‑76 (MQ=255)

                                  caccccATAAACCCTCTAAAAACCCCTAC‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGa                                     <  2:4269886/73‑1 (MQ=255)

                                   accccATTAATCCTCTAAACACCCCAAC‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGAt                                    <  1:5764175/74‑1 (MQ=255)

                                   aGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGAt                                    >  2:4017088/1‑76 (MQ=255)

                                    gCCCTTAAATCCTCTGAACACCCCTAC‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATg                                   <  2:4141687/76‑1 (MQ=255)

                                     cccATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGc                                  >  1:1660163/1‑76 (MQ=255)

                                      cctttAATACTCCGAACACCCCAAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCa                                 <  1:6241610/73‑1 (MQ=255)

                                      ccATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCa                                 <  1:6251962/76‑1 (MQ=255)

                                      ccATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCa                                 >  2:1144650/1‑76 (MQ=255)

                                       cATTAATCCTCTAACCACCCCAAC‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAg                                <  1:14543/76‑1 (MQ=255)

                                       cATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAg                                >  2:5912561/1‑76 (MQ=255)

                                       cATAAATCCTCTAAACACCCCAAC‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAg                                <  1:5260911/76‑1 (MQ=255)

                                        aTTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGa                               >  2:4726124/1‑76 (MQ=255)

                                        aTTAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGa                               >  1:270352/1‑76 (MQ=255)

                                         ttAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGAc                              >  1:5131539/1‑76 (MQ=255)

                                          tAATCCTCGGAACACCCCTAC‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACt                             <  2:1638874/76‑1 (MQ=255)

                                          tAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACt                             >  1:2854808/1‑76 (MQ=255)

                                          tAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACt                             >  1:3104478/1‑76 (MQ=255)

                                          tAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACt                             >  2:5607361/1‑76 (MQ=255)

                                          tAATACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACt                             >  2:1661070/1‑76 (MQ=255)

                                          tAATACTCAAAACACCCCAAC‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACt                             <  2:2175210/76‑1 (MQ=255)

                                           aaTCCTCTGACCACCCCAAC‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTc                            <  2:2506277/76‑1 (MQ=255)

                                           aaTACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTc                            >  1:3267619/1‑76 (MQ=255)

                                            aTACTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCa                           >  2:3328463/1‑76 (MQ=255)

                                              aCTCTGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGc                         >  2:1408749/1‑76 (MQ=255)

                                                tctGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCaa                       >  1:3179242/1‑76 (MQ=255)

                                                 ctGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGCTGCAAACCCAGATGCAGACTCAGCaaa                      >  2:698895/1‑76 (MQ=255)

                                                 ctGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCaaa                      >  2:3455944/1‑76 (MQ=255)

                                                 ctGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCaaa                      >  1:6118660/1‑76 (MQ=255)

                                                 ctGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCaaa                      >  2:1378166/1‑76 (MQ=255)

                                                 ctGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGAATCAGCaaa                      >  2:3346544/1‑76 (MQ=255)

                                                  tGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCaaac                     >  1:3582719/1‑75 (MQ=255)

                                                  tGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAAt                     >  1:5630405/1‑76 (MQ=255)

                                                  tGAACAACCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAAt                     >  2:3670057/1‑76 (MQ=255)

                                                   taaccacCCCAACCCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAAt                     <  2:2654616/75‑1 (MQ=255)

                                                   aaacaccCCTAC‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATc                    <  1:4358483/75‑1 (MQ=255)

                                                    aacaacCGTAA‑CACGCCGGGTAAGAAAATAGCCAGCCTGCAGCGGATGCAAACTCAGATGCACACTAATCAAATcc                   <  2:1478012/76‑1 (MQ=255)

                                                    aacaacCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGCTGCAAACCCAGATGCAGACTCAGCAAATcc                   >  2:1452357/1‑76 (MQ=255)

                                                    aacaacCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATcc                   >  2:1885251/1‑76 (MQ=255)

                                                      caacCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAg                 >  2:2429328/1‑76 (MQ=255)

                                                        ccccTAC‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCa               <  1:3424646/75‑1 (MQ=255)

                                                        acCCTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCa               >  1:2059750/1‑76 (MQ=255)

                                                         cccTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCaa              >  2:2039884/1‑76 (MQ=255)

                                                          ccTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCaaa             >  1:4537113/1‑76 (MQ=255)

                                                           cTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCaaaa            >  2:3765302/1‑76 (MQ=255)

                                                           cTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCaaaa            >  2:4614418/1‑76 (MQ=255)

                                                           cTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCaaaa            >  2:4773869/1‑76 (MQ=255)

                                                           cTAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCaaaa            >  2:5806482/1‑76 (MQ=255)

                                                            tAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCaaaaa           >  2:6123234/1‑76 (MQ=255)

                                                            tAA‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCaaaaa           >  1:1883519/1‑76 (MQ=255)

                                                             aa‑CCAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAg          >  2:4989020/1‑76 (MQ=255)

                                                              acccAGCCGGGGCATAAGATCCCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAg          <  1:2462617/74‑1 (MQ=255)

                                                               acaaGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAgg         >  2:357732/4‑76 (MQ=255)

                                                                ccAGCGGGGGCATCAGATCCCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAggg        <  2:2082226/76‑1 (MQ=255)

                                                                 cAGCCGGGGCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGGa       >  1:1191960/1‑76 (MQ=255)

                                                                 cAGCCGGGCCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAACAAATCCAGCAAAAAGGGa       <  2:360598/76‑1 (MQ=255)

                                                                   gCCGGGGCATCAGATCCCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGGATg     <  1:1297978/76‑1 (MQ=255)

                                                                      ggggCATCAGATCCCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGGATGCTg  <  1:86469/76‑1 (MQ=255)

                                                                      ggggCATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGGATGCTg  <  2:2290879/76‑1 (MQ=255)

TCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAA‑CCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGGATGCTG  >  REL606/3517636‑3517780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`